Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,520,500 Δ1 bp 8.1% intergenic (‑176/+171) yjhU ← / ← yjhF putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,520,5000A. 8.1% 398.9 / 29.3 135intergenic (‑176/+171)yjhU/yjhFputative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter
Reads supporting (aligned to +/- strand):  ref base A (67/56);  new base . (4/7);  total (71/64)
Fisher's exact test for biased strand distribution p-value = 3.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.20e-01

Only 100 of 137 total aligned reads displayed.

AATGCTCATCTTAACCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAACAACAGG  >  NC_000913/4520352‑4520647
                                                                                                                                                    |                                                                                                                                                    
aaTGCTCATCTTAACCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGAc                                                                                                                                                     <  1:118957/150‑1 (MQ=255)
aaTGCTCATCATAACCCTGTTTAACAGGAGAGTTTATGCAGATTGCAATTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGGTCCCCCATACTGTAAAGCCGGAGAc                                                                                                                                                     <  2:349111/150‑1 (MQ=255)
aaTGCACCTCTTAACCCTGTTTACCCGGAGAGGTGATGCAGATTGCCAGTGGCATGATTGTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGAc                                                                                                                                                     <  2:343855/150‑1 (MQ=255)
  tGCTCATCTTAACCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAt                                                                                                                                                   >  2:688560/1‑150 (MQ=255)
  tGCTCATCTTAACCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAt                                                                                                                                                   >  1:1654432/1‑150 (MQ=255)
  tGCTCATCTTAACCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTAGACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCCTTTGAGAGATCGCCCGCTGTTCCCCCAAACTGTAACGCCGGAGACAt                                                                                                                                                   >  2:1807891/1‑150 (MQ=255)
    cTCATCTTAACCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGc                                                                                                                                                <  1:1695498/150‑1 (MQ=255)
      cATCTTAACCCTGTTTAACAGGAGAGTTGATGAAGATTGCAAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACAAGCATACCATTTGAGCGATCGCAAGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTc                                                                                                                                              >  2:971808/1‑150 (MQ=255)
       aTCTTAACCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCTGAGACATGCTcc                                                                                                                                             >  1:523067/1‑150 (MQ=255)
       aTCTTAACCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTcc                                                                                                                                             <  1:871318/150‑1 (MQ=255)
           tAACCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGGGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCt                                                                                                                                         <  1:1432920/150‑1 (MQ=255)
            aaCCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATATACCAGCATACCATTTGAGCGAGCGCCCGCTGTGCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCtt                                                                                                                                        <  1:1989440/150‑1 (MQ=255)
             aCCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCttt                                                                                                                                       <  2:885792/150‑1 (MQ=255)
              cccTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGGTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTa                                                                                                                                      <  2:441348/150‑1 (MQ=255)
                  gTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCAATACTGTAAAGCCGGAGCATGTCTCCGGCTTTACAGt                                                                                                                                  <  1:689533/150‑1 (MQ=255)
                     tACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTGCATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTAtt                                                                                                                               <  2:1839408/150‑1 (MQ=255)
                        ccGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACt                                                                                                                            >  1:1547056/1‑150 (MQ=255)
                        ccGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCTTTACAGTAtgggg                                                                                                                            <  2:1032360/150‑5 (MQ=39)
                        ccGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCTTTACAGTAtgggg                                                                                                                            <  1:424811/150‑5 (MQ=39)
                         cGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTg                                                                                                                           <  2:1267310/150‑1 (MQ=255)
                         cGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTg                                                                                                                           >  2:1975818/1‑150 (MQ=255)
                          ggAGAGTTGATGCAGATTGCCAGTGGCATGATATTATACAGATATTTTATTCTATTACATGAAATAAACCAGCATACCATTTGAGCGATCGACAGCTGTTCCCCCATACTGTAAAGCCGGTGAAATGCTCCGGCTTTACAGTATTACTga                                                                                                                          >  1:296069/1‑150 (MQ=255)
                              agTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACGTGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAAt                                                                                                                      <  2:1359096/150‑1 (MQ=255)
                               gTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATa                                                                                                                     >  1:860374/1‑150 (MQ=255)
                                  gATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATacac                                                                                                                  >  2:309525/1‑150 (MQ=255)
                                   aTGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACAcc                                                                                                                 <  2:1922958/150‑1 (MQ=255)
                                   aTGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACAcc                                                                                                                 <  2:588509/150‑1 (MQ=255)
                                     gCAGATTGCAAGTGGCATGATTTTATACTGATATTTTTTTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCa                                                                                                               <  2:1098466/150‑1 (MQ=255)
                                          ttGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAg                                                                                                          <  2:1687068/150‑1 (MQ=255)
                                          ttGCCAGAGCCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACAAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATAACTGAGAATACACCCATCaac                                                                                                          >  2:1016112/1‑149 (MQ=255)
                                            gCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCa                                                                                                        >  2:1410875/1‑150 (MQ=255)
                                            gCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCa                                                                                                        >  2:725626/1‑150 (MQ=255)
                                            gCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCa                                                                                                        >  1:1837244/1‑150 (MQ=255)
                                             ccAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCAt                                                                                                       >  1:1792436/1‑150 (MQ=255)
                                                  ggCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCg                                                                                                  >  1:1684249/1‑150 (MQ=255)
                                                  ggCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCg                                                                                                  >  1:255824/1‑150 (MQ=255)
                                                        aTTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGtt                                                                                            >  1:1998332/1‑150 (MQ=255)
                                                         ttttATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCGTCAAGCATAATCGAAGGTTc                                                                                           <  2:1968579/150‑1 (MQ=255)
                                                          tttATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCa                                                                                          <  1:1466840/150‑1 (MQ=255)
                                                                      ttttATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCtt                                                                              <  2:462488/150‑1 (MQ=255)
                                                                        ttATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCtttt                                                                            <  1:1080151/150‑1 (MQ=255)
                                                                        ttATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCtttt                                                                            <  2:768133/150‑1 (MQ=255)
                                                                          aTTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCtttttt                                                                          >  2:1633465/1‑149 (MQ=255)
                                                                           ttCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGa                                                                         >  1:1169278/1‑150 (MQ=255)
                                                                            tCTTTTACATTAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGATTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGTAGGTTCAATATCGCCCCTTTTTGAt                                                                        <  2:1220151/150‑1 (MQ=255)
                                                                             cTTTTACATGAAATAAACCAGCCTACCTTTTGAGCGATCGCCCGCTGTTCCCCCATCCTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATc                                                                       <  1:762958/150‑1 (MQ=255)
                                                                             cTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGACTACCCCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATc                                                                       >  2:1894409/1‑150 (MQ=255)
                                                                             cTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATc                                                                       <  2:1569911/150‑1 (MQ=255)
                                                                             cTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATAGCCCCTTTTTGATc                                                                       >  2:1100079/1‑150 (MQ=255)
                                                                              ttttACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCg                                                                      <  1:62651/150‑1 (MQ=255)
                                                                              ttttACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCg                                                                      <  2:350827/150‑1 (MQ=255)
                                                                              ttttACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCg                                                                      <  2:692690/150‑1 (MQ=255)
                                                                              ttttACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCg                                                                      <  2:1603833/150‑1 (MQ=255)
                                                                              ttttACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCg                                                                      <  2:1612224/150‑1 (MQ=255)
                                                                                   cATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGc                                                                 >  2:275523/1‑150 (MQ=255)
                                                                                   cATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGc                                                                 >  2:1527693/1‑150 (MQ=255)
                                                                                     tGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGa                                                               >  2:1653497/1‑150 (MQ=255)
                                                                                         aTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCg                                                           <  2:673997/150‑1 (MQ=255)
                                                                                         aTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCg                                                           <  2:686878/150‑1 (MQ=255)
                                                                                          tAAACCAGCATACCATTTGCGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCTATATAGCCCCTTTTTGATCGCCAGCGAATCGt                                                          >  2:325110/1‑150 (MQ=255)
                                                                                           aaaCCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGGATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTc                                                         >  1:1550580/1‑150 (MQ=255)
                                                                                            aaCCAGCATACCATTTGAGCGATCGCCCTCTGTTCCCCCATACTGTAAAGACGGAGACATGATCCGGCTTTACAGTATTAATGAGAATACACCGCTCAAGCATAATCGAAGGCTCAATATCGACACTTATTGATCGACAGCGATTCGtaa                                                        >  2:849177/1‑148 (MQ=255)
                                                                                               cAGCATACCATTTGAGCGAGCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCg                                                     <  1:654196/150‑1 (MQ=255)
                                                                                                  aaTACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCAGGAGACATGCTCGGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGc                                                  <  1:271141/149‑1 (MQ=255)
                                                                                                      ccATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGAc                                              <  1:761444/150‑1 (MQ=255)
                                                                                                       cATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATTGCCAGCGATTCGTCACCGTGCAGACg                                             >  2:760539/1‑150 (MQ=255)
                                                                                                              gCGATCGCCCGCTGTTCCGCCATACTGTTAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACAGCCATCAAGCATAATCGAAGGTTGTATATCGCCCCTTTTTGATCGCCTGCGATTCGTCACCGTGCTGACGGTTGCCg                                      <  2:1623494/150‑1 (MQ=255)
                                                                                                              gCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCg                                      >  1:1512621/1‑150 (MQ=255)
                                                                                                              gCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCg                                      >  2:725589/1‑150 (MQ=255)
                                                                                                                gATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATACTCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGtc                                    <  1:345104/150‑1 (MQ=255)
                                                                                                                gATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGAAATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGAATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGACAGCGATTCGTCACCGTGCAGACGGTTGCCGtc                                    >  2:912041/1‑150 (MQ=255)
                                                                                                                 ttCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGTCATGCTCCGGCGTTACATTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGtca                                   <  1:971808/149‑1 (MQ=255)
                                                                                                                 aTCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGtca                                   >  2:1435838/1‑150 (MQ=255)
                                                                                                                   cGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTCCAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCGTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGGTGCCGtcacg                                 >  1:417409/1‑149 (MQ=255)
                                                                                                                    gCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGtcactc                                <  1:342289/150‑1 (MQ=255)
                                                                                                                       cGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTcactcact                             >  2:623719/1‑150 (MQ=255)
                                                                                                                           gTTCCCCCATACTGTAAAGCCGGACACATGCACCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGGCGTCACTCACTAAAt                         <  1:1016112/150‑1 (MQ=255)
                                                                                                                              cccccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTAt                      >  1:656066/1‑150 (MQ=255)
                                                                                                                                cccATACTTTAAAGCCGTAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCATGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGGCACCGGGCAGACGGTTGCCGTCACTCACTAAATTATgg                    <  1:1317823/150‑1 (MQ=255)
                                                                                                                                 ccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCCAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATggg                   <  1:750059/150‑1 (MQ=255)
                                                                                                                                 ccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGGCACTCACTAAATTATggg                   <  2:1726976/150‑1 (MQ=255)
                                                                                                                                   aTACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATggggg                 >  2:806771/1‑150 (MQ=255)
                                                                                                                                    tACTGTAAAGCCAGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAATCATAATCGAAGTTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGa                >  2:1045244/1‑150 (MQ=255)
                                                                                                                                       tGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACCCCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAg             >  2:955900/1‑150 (MQ=255)
                                                                                                                                       tGTAAAGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAg             >  1:440998/1‑150 (MQ=255)
                                                                                                                                        gTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGt            >  1:132200/1‑150 (MQ=255)
                                                                                                                                        gTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGt            >  1:733450/1‑150 (MQ=255)
                                                                                                                                        gTAAAGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATTGGGTACAGt            >  2:1665610/1‑150 (MQ=255)
                                                                                                                                         tAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTg           <  2:1837244/150‑1 (MQ=255)
                                                                                                                                          aaaGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGa          <  1:1887769/150‑1 (MQ=255)
                                                                                                                                          aaaGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGa          <  1:726456/150‑1 (MQ=255)
                                                                                                                                          aaaGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGa          >  1:1638967/1‑150 (MQ=255)
                                                                                                                                          aaaGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGGTGCCGTCACTCACTAAATTATGGGGGACAGTGa          >  1:1641679/1‑150 (MQ=255)
                                                                                                                                            aGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACGCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGaac        <  2:225288/150‑1 (MQ=255)
                                                                                                                                            aGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACCCCCATCAAGCATGATCGAAGGTTCAATATAGCCCCTTTATGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATTGGGGAAAGTGaac        >  1:1303352/1‑150 (MQ=255)
                                                                                                                                             gCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGaaca       <  1:1635866/150‑1 (MQ=255)
                                                                                                                                             gCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGaaca       >  1:490825/1‑150 (MQ=255)
                                                                                                                                             gCCGGAGACATGCTCCGGCTGTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCCCCGTGCAGACGGTTGCCGTCCCTCACTAAATTATGGGGGACAGTGacca       >  1:1085443/1‑150 (MQ=255)
                                                                                                                                                agagaCATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACTGTGCAGACGGTTGCCGTCACTCAATAAATTATGGGGGACAGTGAacaaca    >  1:132363/2‑150 (MQ=255)
                                                                                                                                                  agaCATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAACAACAgg  <  2:1733470/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AATGCTCATCTTAACCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAACAACAGG  >  NC_000913/4520352‑4520647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: