Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,299,267:1 +A 5.2% intergenic (+22/‑1915) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,299,2671.A 5.2% 459.1 / 19.4 115intergenic (+22/‑1915)ychE/oppAUPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base . (52/57);  new base A (3/3);  total (55/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

Only 100 of 116 total aligned reads displayed.

TGGTACGGGTTTTACGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTT  >  NC_000913/1299118‑1299413
                                                                                                                                                      |                                                                                                                                                  
tGGTACGGGTTTTACGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGc                                                                                                                                                     >  2:1013768/1‑150 (MQ=255)
     cGGGTTTTACGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCa                                                                                                                                               >  2:414916/1‑150 (MQ=255)
        gTTTTACGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGc                                                                                                                                            >  1:1239638/1‑150 (MQ=255)
         ttttACGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCt                                                                                                                                           <  1:628653/150‑1 (MQ=255)
          tttACGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTc                                                                                                                                          >  2:301567/1‑150 (MQ=255)
          tttACGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTc                                                                                                                                          >  2:1833900/1‑150 (MQ=255)
           ttACGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGTTGGCATTGGGGTTTGTATTTATCGTTAATTGTATTTAGTGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACTGAGCTGCCATGCTcc                                                                                                                                         <  1:664384/150‑1 (MQ=255)
           ttACGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTcc                                                                                                                                         >  2:1494096/1‑150 (MQ=255)
              cGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCAATGCTCCGtt                                                                                                                                      >  1:1631263/1‑150 (MQ=255)
               gCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGttt                                                                                                                                     <  2:1175397/150‑1 (MQ=255)
                ccAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTTCTGATGGCATTGGGGATTGATTTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTa                                                                                                                                    >  2:1759611/1‑150 (MQ=255)
                 cAGTCCGGCATCAACGTGATTACGCTTATTATGGCGCTATAGCTGATGGCATTGTGGATTGAATTTATCGTTACTGTTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCATGGCAGCTCCGtttca                                                                                                                                   <  2:1223518/150‑3 (MQ=255)
                   gACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCATGGCAGCTCCGtttcatt                                                                                                                                 <  2:775521/150‑5 (MQ=255)
                     ccGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCg                                                                                                                               >  1:1030207/1‑150 (MQ=255)
                      cGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGTGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGt                                                                                                                              <  1:1124450/150‑1 (MQ=255)
                        gCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCa                                                                                                                            <  1:1198403/150‑1 (MQ=255)
                         cATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATACCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCat                                                                                                                           >  2:837506/1‑150 (MQ=255)
                          aTCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCatt                                                                                                                          >  2:290146/1‑150 (MQ=255)
                          aTCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCAACTGACTGCTTAATTAATTCCTTTCAAATGAAAAGGAGCTGCAATGCTCCGTATACTTCGTCatt                                                                                                                          >  1:171826/1‑150 (MQ=255)
                            cAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCattat                                                                                                                        >  2:1653123/1‑150 (MQ=255)
                             aaCGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCattatt                                                                                                                       >  1:1905024/1‑150 (MQ=255)
                                gTGCTTACGCGTTATATGGGGCTATTGCTGAGGGCCTTGGGGAATGAATTTATCGTTACCCTTATAAAGGGTATTTTCCCCGGCCTGCTTAATTAACTCCTTTCAAATTAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATAAttttt                                                                                                                    <  2:367980/150‑1 (MQ=255)
                                 tGATTACGCGTATTATGGGGATATTGCTGATGGAATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTa                                                                                                                   <  2:813871/150‑1 (MQ=255)
                                  gATTACGCGTATTATGGGGCTATTTCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTAc                                                                                                                  <  1:8036/150‑1 (MQ=255)
                                      aCGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTg                                                                                                              <  2:1600483/150‑1 (MQ=255)
                                       cgcgTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGt                                                                                                             <  1:637635/150‑1 (MQ=255)
                                              tatGGGGCTATTGCTGATGGCATTGGGGTTTGAATTTATGGTTACTTGTATTAAGGGGATTTTCCGCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCcgcg                                                                                                      <  2:1098644/150‑1 (MQ=255)
                                               atGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCgcgc                                                                                                     <  2:232999/150‑1 (MQ=255)
                                                      tATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATc                                                                                              <  2:1957130/150‑1 (MQ=255)
                                                      tATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATc                                                                                              <  2:70373/150‑1 (MQ=255)
                                                        ttGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATcaa                                                                                            >  2:1431391/1‑150 (MQ=255)
                                                           cTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATcaaaag                                                                                         <  2:1003972/150‑1 (MQ=255)
                                                             gATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATcaaaagca                                                                                       <  1:626928/150‑1 (MQ=255)
                                                              aTGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATcaaaagcaa                                                                                      <  2:1792101/150‑1 (MQ=255)
                                                              aTGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATcaaaagcaa                                                                                      >  1:1074648/1‑150 (MQ=255)
                                                              aTGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAAGTAATTCCTTTCAAATGAAACTGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATcaaaagcaa                                                                                      >  1:86405/1‑150 (MQ=255)
                                                                  cATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAgg                                                                                  <  1:590833/150‑1 (MQ=255)
                                                                    ttGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGaa                                                                                >  1:1917186/1‑150 (MQ=255)
                                                                      ggggATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATa                                                                              <  2:380098/150‑1 (MQ=255)
                                                                      ggggATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATa                                                                              <  1:161176/150‑1 (MQ=255)
                                                                      ggggATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTACTTTCAAAAGAAACGCAGCAGCCATGCTCCGATTACTTCGTCATTATTTTTACTATGTTCCCGCGCAGTTATCAAAACCAAAAGTAATa                                                                              >  2:779430/1‑150 (MQ=255)
                                                                        ggATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCGTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAgg                                                                            >  1:1068673/1‑150 (MQ=255)
                                                                        ggATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAgg                                                                            >  1:245702/1‑150 (MQ=255)
                                                                          aTTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTTTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTa                                                                          >  2:580649/1‑150 (MQ=255)
                                                                                  tATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTTTCAAAAGCAAAAGGAATAGGTAAAAATAtt                                                                  >  1:71586/1‑150 (MQ=255)
                                                                                  tATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATAtt                                                                  <  2:138010/150‑1 (MQ=255)
                                                                                   aTCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATAttc                                                                 <  1:440779/150‑1 (MQ=255)
                                                                                   aTCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATAttc                                                                 <  1:414916/150‑1 (MQ=255)
                                                                                      gTTACTGGTATTAAGGGGATTTTCCCCGACCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATAttcttc                                                              >  2:1052856/1‑150 (MQ=255)
                                                                                      gTTACTGGTATTAAGGGGATTTTCACCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATAttcttc                                                              <  1:1290294/150‑1 (MQ=255)
                                                                                         aCTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAATGGAATAGTTAAAAATATTCTTCTCa                                                           >  1:1235384/1‑150 (MQ=255)
                                                                                          cTGGTATTAAGGGTATTTTCCCCGGCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCaaa                                                         <  2:873511/150‑1 (MQ=255)
                                                                                          cTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCaa                                                          >  2:1286677/1‑150 (MQ=255)
                                                                                            ggTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCAATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGAAGTTATCAAAAGCAAAAGGAATAGGTAATAATATTCTTCTCAAAt                                                        >  1:1265201/1‑150 (MQ=255)
                                                                                             gTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAAtt                                                       <  1:1494096/150‑1 (MQ=255)
                                                                                                 tAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACag                                                   <  2:1971374/150‑1 (MQ=255)
                                                                                                      ggATTTTCCCCGGCCTGCTTTATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTTCTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACagttagt                                              <  1:1770296/150‑1 (MQ=255)
                                                                                                      ggATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTTAAAATATTCTTCTCAAATTACagttagt                                              >  1:1663034/1‑150 (MQ=255)
                                                                                                        aTTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAgttagtta                                            <  2:541211/150‑1 (MQ=255)
                                                                                                         ttttCCCCGGCCTGCTTAATTTATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTtat                                           >  1:323270/1‑150 (MQ=255)
                                                                                                             ccccGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAgg                                       >  2:290336/1‑150 (MQ=255)
                                                                                                              cccGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGa                                      >  2:469739/1‑150 (MQ=255)
                                                                                                              cccGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCAGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGa                                      >  2:1295355/1‑150 (MQ=255)
                                                                                                                 ggCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGAttt                                   <  1:1973116/150‑1 (MQ=255)
                                                                                                                  gCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTc                                  >  1:67987/1‑150 (MQ=255)
                                                                                                                  gCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTc                                  <  1:574035/150‑1 (MQ=255)
                                                                                                                  gCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTc                                  >  1:468905/1‑150 (MQ=255)
                                                                                                                   ccTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTcc                                 <  1:1689795/150‑1 (MQ=255)
                                                                                                                     tGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCtt                               <  2:602683/150‑1 (MQ=255)
                                                                                                                     tGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCtt                               <  1:180282/150‑1 (MQ=255)
                                                                                                                      tcTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAACAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTa                              <  1:301567/149‑1 (MQ=255)
                                                                                                                      gCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTa                              >  1:1546005/1‑150 (MQ=255)
                                                                                                                      gCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTa                              <  1:1808291/150‑1 (MQ=255)
                                                                                                                         taattaatTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACt                           <  1:1204690/150‑1 (MQ=255)
                                                                                                                         taattaatTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACt                           <  2:1271960/150‑1 (MQ=255)
                                                                                                                          aattaattCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTg                          <  2:1239638/150‑1 (MQ=255)
                                                                                                                            ttcattCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCt                        <  2:680374/147‑1 (MQ=255)
                                                                                                                              aattCCTTTCAAATGAAACGGAGCTGCAATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTtc                      <  1:1013768/150‑1 (MQ=255)
                                                                                                                               attCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTtct                     <  1:1801423/150‑1 (MQ=255)
                                                                                                                               attCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTtct                     <  1:1689136/150‑1 (MQ=255)
                                                                                                                               attCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTtct                     >  2:600741/1‑150 (MQ=255)
                                                                                                                               attCCTATCAAATGAAACGGAGCTGCCAAGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTtct                     <  1:192331/150‑1 (MQ=255)
                                                                                                                                   cTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTctcctc                 <  2:1905024/150‑1 (MQ=255)
                                                                                                                                   cTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTctcctc                 <  1:1653123/150‑1 (MQ=255)
                                                                                                                                   cTTTCAAATGAAACGGAGCTGCAATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAGTTACAGTTTGTTATATGGTTTTCCTTAACTGCTTctcctc                 >  1:269258/1‑150 (MQ=255)
                                                                                                                                    tttCAAATGACACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCa                <  1:1085829/150‑1 (MQ=255)
                                                                                                                                    tttCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCa                <  2:1817101/150‑1 (MQ=255)
                                                                                                                                         aaTGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACcatc           >  2:483784/1‑150 (MQ=255)
                                                                                                                                          agtaaaCGGAGCATGGCAGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCATCACcaaca          >  1:128177/4‑150 (MQ=255)
                                                                                                                                          aTGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACcatca          <  1:332277/150‑1 (MQ=255)
                                                                                                                                          aTGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACcatca          >  2:590492/1‑150 (MQ=255)
                                                                                                                                          aTGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACcatca          <  1:578246/150‑1 (MQ=255)
                                                                                                                                            taaaCGGAGCATGGCAGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATg        >  1:1111561/2‑150 (MQ=255)
                                                                                                                                            gAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATg        >  2:1963697/1‑150 (MQ=255)
                                                                                                                                             aaaCGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGt       >  1:1381672/1‑150 (MQ=255)
                                                                                                                                               aCGGAGCTGCCCTGCTCCGTTTACTTCGGCATTATTTTTACTTTGTTCCCGCGCAGGTATCAAAAGCAAAAGGAAGTGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTa     >  2:1515107/1‑150 (MQ=255)
                                                                                                                                               aCGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTa     <  2:1631263/150‑1 (MQ=255)
                                                                                                                                               aCGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAAAATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTa     <  2:1622882/150‑1 (MQ=255)
                                                                                                                                                 ggAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATTTTAtt   >  2:771026/1‑150 (MQ=255)
                                                                                                                                                 ggAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTAATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTCCAGTTAGTTTTAAGGATTTACTTAACTGATTCTCCTCACCATCATGTTAtt   >  1:1233676/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
TGGTACGGGTTTTACGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTT  >  NC_000913/1299118‑1299413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: