Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 175,149:1 +G 7.0% coding (43/1422 nt) clcA → H(+)/Cl(‑) exchange transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913175,1491.G 7.0% 507.3 / 36.9 128coding (43/1422 nt)clcAH(+)/Cl(‑) exchange transporter
Reads supporting (aligned to +/- strand):  ref base . (57/62);  new base G (5/4);  total (62/66)
Fisher's exact test for biased strand distribution p-value = 7.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.81e-01

Only 100 of 135 total aligned reads displayed.

TATAAAAGTTTTGTGCATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACG  >  NC_000913/175000‑175297
                                                                                                                                                      |                                                                                                                                                    
tataAAAGTTTTGTGCATTTGAAACATTACGCTTTGCAACGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCcgcgc                                                                                                                                                       <  2:657060/150‑1 (MQ=255)
  taAAAGTTTTGTGCATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCgcgcgc                                                                                                                                                    >  2:412768/1‑150 (MQ=255)
   aaaaGTTTTGTGCATTTAAAACATTACGCTTTGCAAAGGATTTTCATGGAAAGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACGAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGcc                                                                                                                                                   <  2:545177/150‑1 (MQ=255)
      aGTTTTGTGCATTTGAAACATTACGCGTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGc                                                                                                                                                <  2:1533943/150‑1 (MQ=255)
       gTTTTGTGCATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCg                                                                                                                                               >  1:1282399/1‑150 (MQ=255)
        ttttGTGCATTTGAAACAATACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCGCGCGGCCTGCgg                                                                                                                                              <  2:1510468/150‑2 (MQ=255)
         tttGTGCATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGAc                                                                                                                                             <  1:703264/150‑1 (MQ=255)
         tttGTGCATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCGCGCGGCCTGCggt                                                                                                                                             <  2:956576/150‑3 (MQ=255)
         tttGTGCATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCGCGCGGCCTGCggt                                                                                                                                             <  1:1258359/150‑3 (MQ=255)
          ttGTGCATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCGCGCGGCCTGCggtg                                                                                                                                            <  2:982275/150‑4 (MQ=255)
          ttGTGCATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTAATTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACg                                                                                                                                            <  2:1454535/150‑1 (MQ=255)
           tgtgCATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCggtgt                                                                                                                                           <  1:1138620/150‑5 (MQ=255)
            gtgCATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCATGCCGCGCGCCTGCGACGCa                                                                                                                                          >  1:349259/1‑150 (MQ=255)
            gtgCATTTGAAACATTACGCTTGGCAAAGGATTATCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCa                                                                                                                                          <  2:470506/150‑1 (MQ=255)
             tgaaTTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAg                                                                                                                                         <  2:741913/147‑1 (MQ=255)
             tgCATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGTAg                                                                                                                                         >  1:1711512/1‑150 (MQ=255)
              gCATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGCACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATCCTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGa                                                                                                                                        >  1:309892/1‑150 (MQ=255)
               cATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGAc                                                                                                                                       >  1:1301329/1‑150 (MQ=255)
               cATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGAc                                                                                                                                       <  1:855647/150‑1 (MQ=255)
               cATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGAc                                                                                                                                       >  1:1847754/1‑150 (MQ=255)
                aTTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGAGAGAAAAATGCCGTCATCTTATTTGTATGACCAAAAAGTGATCATTGGATTAAAACAGATACTCCCTCATTAGAAACAACACAGACCTCGCGCCTGAGACGCAGACa                                                                                                                                      >  2:1536680/1‑150 (MQ=255)
                   tGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACt                                                                                                                                   <  2:1847754/150‑1 (MQ=255)
                    gAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCTCGCCTGCGACGCAGACAACTg                                                                                                                                  >  1:1917767/1‑150 (MQ=255)
                       aCATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGGCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGAtt                                                                                                                               <  2:1464444/150‑1 (MQ=255)
                        cATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCTACGCAGACAACTGATTc                                                                                                                              >  1:1233704/1‑150 (MQ=255)
                           tACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGcc                                                                                                                           <  2:2013251/150‑1 (MQ=255)
                                 ttGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAActtc                                                                                                                     >  1:1631140/1‑150 (MQ=255)
                                  tGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGACACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAActtct                                                                                                                    >  2:907059/1‑150 (MQ=255)
                                      aaGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAg                                                                                                                <  1:514594/150‑1 (MQ=255)
                                       aGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAgc                                                                                                               <  2:498182/150‑1 (MQ=255)
                                        ggATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCGTTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAgcg                                                                                                              >  2:764880/1‑150 (MQ=255)
                                        ggATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAgcg                                                                                                              <  2:1271202/150‑1 (MQ=255)
                                            tttCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGAt                                                                                                          <  1:897681/150‑1 (MQ=255)
                                             ttCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATa                                                                                                         <  2:1538505/150‑1 (MQ=255)
                                               cATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACACCCTCTTTAGAAACACCGACGGCCGCGCGCCTGCGCCGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATaaa                                                                                                       >  1:1347316/1‑150 (MQ=255)
                                                aTGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACACCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTAGCCAACTTCTTGAGCGCGATaaaa                                                                                                      >  1:1769787/1‑150 (MQ=255)
                                                 tGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAAc                                                                                                     <  1:1881256/150‑1 (MQ=255)
                                                    aaCGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAAcccc                                                                                                  >  1:501569/1‑150 (MQ=255)
                                                       gTGCGAGTAAAATGCCGTCATCTTAGTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGtt                                                                                               <  1:1014853/150‑1 (MQ=255)
                                                        tGCGAGTAAAATGCCGTCAGCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGACTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGCTAAAACACCGTTa                                                                                              >  1:1679957/1‑150 (MQ=255)
                                                         gCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAg                                                                                             <  2:1112361/150‑1 (MQ=255)
                                                           gAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCGCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGcc                                                                                           <  2:1193247/150‑1 (MQ=255)
                                                            aGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCa                                                                                          >  2:1875887/1‑150 (MQ=255)
                                                                aaaTGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCAtttt                                                                                      <  2:1213569/150‑1 (MQ=255)
                                                                 aaTGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTg                                                                                     <  1:1623729/150‑1 (MQ=255)
                                                                   tGCCGTCTTCTTATTTTTATGAGAAATAAGTTATCATTGGTTTATATCTGTTACTCCCTCTTTAGATACACCGCAGGACGCTCGCCTGGGACGCAGACAACTGATTCGCGAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGtt                                                                                   <  1:1536680/150‑1 (MQ=255)
                                                                   tGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGtt                                                                                   <  1:195661/150‑1 (MQ=255)
                                                                   tGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGtt                                                                                   <  1:102894/150‑1 (MQ=255)
                                                                    gCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACACCGTTAGACATTTTGttt                                                                                  >  2:1363424/1‑150 (MQ=255)
                                                                       gTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTCAGAAACACCGCAGGCCGCGCGCCTGAGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATg                                                                               <  2:1193485/150‑1 (MQ=255)
                                                                        tCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATgg                                                                              <  1:1162959/150‑1 (MQ=255)
                                                                         cATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATggc                                                                             >  2:1042562/1‑150 (MQ=255)
                                                                          gtCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTTGCCATTTTGTTTATggcg                                                                            >  1:488989/2‑150 (MQ=255)
                                                                            cTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATggcggc                                                                          <  1:1100/150‑1 (MQ=255)
                                                                            cTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATggcggc                                                                          >  2:1685255/1‑150 (MQ=255)
                                                                                 ttGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTCGCCAGTTTGTTTATGGCGGCAgtcg                                                                     >  1:29219/1‑150 (MQ=255)
                                                                                  tGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAgtcgt                                                                    <  1:1935013/150‑1 (MQ=255)
                                                                                   gTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACAGCAGGCCGCGCGCCTGCGACGCGGACAACTGATTCGCCAACTGCTTGAGCGCGATACTGCCGCGTTAGCGATTTTGTTTAAGGCGGCAgtcgtc                                                                   >  2:1287536/1‑150 (MQ=255)
                                                                                     aTGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCgg                                                                 <  1:1480055/150‑1 (MQ=255)
                                                                                       gACCAATAAGTGATCATTGGATGAAAGCTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCa                                                               >  2:1590317/1‑150 (MQ=255)
                                                                                       gACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAACCACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCa                                                               >  2:910563/1‑150 (MQ=255)
                                                                                        aCCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGCGCGCGATAAAACCCCGTTAGCCATTTTGTGTATGGCGGCAGTCGTCGGCAc                                                              >  2:849349/1‑150 (MQ=255)
                                                                                         ccacttaGTGATATTTGGATGAAAAGTGATACTCCCTCATTAGAAATACCGAAGGCCGCGGGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACg                                                             <  2:1471043/144‑1 (MQ=255)
                                                                                          cAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGc                                                            >  2:904117/1‑150 (MQ=255)
                                                                                          cAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGc                                                            >  2:1195696/1‑150 (MQ=255)
                                                                                              aaGTGATCAGTGGATGAAAACTGATTCTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGCTTCGCCATCTTCTTGAGCGCGATAAACCCGCGTTAGCCTTTTTGTTTATGGCGGCTGTCGTCGGCACGGttgg                                                        >  2:411320/1‑149 (MQ=255)
                                                                                                gTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCttgttg                                                      >  2:675753/1‑150 (MQ=255)
                                                                                                     cATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTg                                                 >  1:968504/1‑150 (MQ=255)
                                                                                                      aTTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTgg                                                >  1:1737029/1‑150 (MQ=255)
                                                                                                      aTTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTgg                                                >  2:1495351/1‑150 (MQ=255)
                                                                                                      aTTGGATGAAAAATGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTgg                                                <  2:133937/150‑1 (MQ=255)
                                                                                                          gATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATATAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGc                                            >  1:1732797/1‑150 (MQ=255)
                                                                                                            tGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCgg                                          <  1:1529848/150‑1 (MQ=255)
                                                                                                                aaCTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGc                                      <  1:898776/150‑1 (MQ=255)
                                                                                                                aaCTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGc                                      <  2:691038/150‑1 (MQ=255)
                                                                                                                   tGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCttt                                   <  2:1872684/150‑1 (MQ=255)
                                                                                                                    gATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCtttt                                  <  1:1673096/150‑1 (MQ=255)
                                                                                                                       aCTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGAc                               <  2:843469/150‑1 (MQ=255)
                                                                                                                            ctctTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCGATTTTGTTTATGGCGGCAGTCGTCGGCGCGCTTGTTCGGCAGGCAGCGGTTGCTTTTGACTAAgg                          >  2:798551/1‑150 (MQ=255)
                                                                                                                             tctTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCTTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGgt                         <  1:42006/150‑1 (MQ=255)
                                                                                                                               tttAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGgtgt                       >  1:1610929/1‑150 (MQ=255)
                                                                                                                                 tAGACACACCGCAGGCCGCGCGCCTGCGCCGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAACACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGGTGGGCTGGCAGCGGTTGCTGTTGACAAAGGTGTCg                     >  2:796660/1‑150 (MQ=255)
                                                                                                                                 tAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCCCGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCg                     >  1:30470/1‑150 (MQ=255)
                                                                                                                                   tctgcgtcGCAGGCGCGCGGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCAGGCAGCGGTTGCTTTTGACAAAGGTGTCGcc                   >  2:1739444/8‑150 (MQ=255)
                                                                                                                                   tctgcgtcGCAGGCGCGCGGCCTGCGACGCAGACAACTGATTCGCCAACATCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCATTTGACAAACGTGTCGcc                   >  2:1739447/8‑150 (MQ=255)
                                                                                                                                   gAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGcc                   >  1:1585979/1‑150 (MQ=255)
                                                                                                                                     tgcgtcGCAGGCGCGCGGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTg                 >  1:1365817/6‑150 (MQ=255)
                                                                                                                                      acacCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTgg                <  2:501569/150‑1 (MQ=255)
                                                                                                                                       cacCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGt               <  2:1631140/150‑1 (MQ=255)
                                                                                                                                        gtcGCAGGCGCGCGGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGtt              >  2:578819/3‑150 (MQ=255)
                                                                                                                                         ccGCAGGCCGCGCGCCTGCGACGCAGACGACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTg             >  1:373976/1‑150 (MQ=255)
                                                                                                                                         ccGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTg             >  2:385623/1‑150 (MQ=255)
                                                                                                                                          cGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGc            <  2:540924/150‑1 (MQ=255)
                                                                                                                                           gCAGGCCGCGCGCCTGCGACGCAGACAACTTATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCa           <  2:1913083/150‑1 (MQ=255)
                                                                                                                                           gCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTATTGAGCGCGATAAAACCCCGTTAGCCATTATGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCa           <  2:1206880/150‑1 (MQ=255)
                                                                                                                                              ggCCGCGAGCCTGCGACGCCGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTCTGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGaa        <  2:1997679/150‑1 (MQ=255)
                                                                                                                                                ccGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACTAAGGTGTCGCCTGGTTGCAGCAcc      >  2:362085/1‑150 (MQ=255)
                                                                                                                                                ccGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAAcc      >  2:330973/1‑150 (MQ=255)
                                                                                                                                                 cgcgcgCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCa     <  2:1585979/150‑1 (MQ=255)
                                                                                                                                                    gcgcCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACg  <  1:910563/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
TATAAAAGTTTTGTGCATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACG  >  NC_000913/175000‑175297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: