Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,868,865:1 +G 5.5% intergenic (+23/‑90) yeaG → / → yeaH protein kinase, endogenous substrate unidentified; autokinase/UPF0229 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,868,8651.G 5.5% 520.5 / 27.1 127intergenic (+23/‑90)yeaG/yeaHprotein kinase, endogenous substrate unidentified; autokinase/UPF0229 family protein
Reads supporting (aligned to +/- strand):  ref base . (59/61);  new base G (5/2);  total (64/63)
Fisher's exact test for biased strand distribution p-value = 4.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

Only 100 of 127 total aligned reads displayed.

CGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCA  >  NC_000913/1868721‑1869013
                                                                                                                                                 |                                                                                                                                                    
cGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCg                                                                                                                                                 >  2:747899/1‑150 (MQ=255)
  tCAACCGACGCGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCGACACCCGTAAACAGGTGCGTTTACTGGGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCggg                                                                                                                                               <  1:842211/150‑1 (MQ=255)
  tCAACAGACGAGCAGACGAAACCCGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACCCCCGTACCCAGGTGCGTTTACTGTGCGAATGGTCTTTGCGCGTACGTAACTCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCggg                                                                                                                                               <  2:1744897/150‑1 (MQ=255)
   cAACCGACGAGCAGAAGAAACACGCCGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGc                                                                                                                                              <  2:368095/150‑1 (MQ=255)
   cAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGc                                                                                                                                              >  1:1775220/1‑150 (MQ=255)
    aaCCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGcc                                                                                                                                             <  1:1818259/150‑1 (MQ=255)
    aaCCGACGAGCAGAAGAAACACGACGACGTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTGTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGcc                                                                                                                                             <  1:615961/150‑1 (MQ=255)
           gaGCAGAAGAAACACGCCGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGGGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACg                                                                                                                                      <  1:1750712/150‑1 (MQ=255)
           gaGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACAGGGCCTACAACg                                                                                                                                      >  2:1878660/1‑150 (MQ=255)
           gaGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTAACATGTGTACCGGGccagggttg                                                                                                                                      <  1:787856/150‑8 (MQ=255)
            aGCAGAATAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGa                                                                                                                                     <  1:536549/150‑1 (MQ=255)
             gCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGAc                                                                                                                                    <  2:186573/150‑1 (MQ=255)
                gaagaaACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGc                                                                                                                                 >  2:1443575/1‑150 (MQ=255)
                gaagaaACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGc                                                                                                                                 >  2:1735289/1‑150 (MQ=255)
                  agaaACACGACGCCTTTGTCGACCGAATGATGGATAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCAGCCCCGGTACACATGTTAACGGGCCTACAACGACAGCGa                                                                                                                               >  2:62191/1‑150 (MQ=255)
                  agaaACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAgaga                                                                                                                               >  2:1487216/1‑150 (MQ=255)
                  agaaACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCGACTACGACAGCgc                                                                                                                               >  1:106513/1‑149 (MQ=255)
                     aaCACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCACGGTACACATGTTACCGGGCCTACAACGACAGCGAAcc                                                                                                                            >  2:1249335/1‑150 (MQ=255)
                       cacGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACACGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAAACgg                                                                                                                          >  1:1466979/1‑149 (MQ=255)
                         cgacgaCTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTgg                                                                                                                        <  2:323185/150‑1 (MQ=255)
                          gacgacTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACACCGACAGCGACCCGTggg                                                                                                                       >  2:834511/1‑150 (MQ=255)
                           acgacTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGc                                                                                                                      <  1:1016230/150‑1 (MQ=255)
                                  tGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGATTGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTCCCGGGCCTACAACGCCAGCGCTCCGTGGGCCTgagag                                                                                                               >  2:109589/1‑149 (MQ=255)
                                   gTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAg                                                                                                              >  2:961136/1‑150 (MQ=255)
                                   gTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAg                                                                                                              >  2:97633/1‑150 (MQ=255)
                                    tCGACCGTATGATGGAAAAGGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACACCGACAGCGAACCGTGGGCCTGAGAAGc                                                                                                             >  2:381990/1‑150 (MQ=255)
                                     cGACCGTATGATGGAAAATGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAAGCGTCTTAACAACCCTGGCCCGTTACTCATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCg                                                                                                            <  1:1980220/150‑1 (MQ=255)
                                      gACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGGGCGATTGGTATTTGCGCGTACGTAAATCGTCTTAACAAGCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTTAGAAGCgg                                                                                                           <  1:92492/150‑1 (MQ=255)
                                       aCCGTATGATGGAAAACGGCTACACCCGTAAACAGGTGCATTTACTGTGCGAATGGTATTTGCGCGTACTTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGc                                                                                                          <  2:1684858/150‑1 (MQ=255)
                                       aCCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGc                                                                                                          <  1:613566/150‑1 (MQ=255)
                                       aCCGGATGATGGAAAAAGGCTACACCCGTAAACTGGGGCGTTTACTGTGCGAATGGTATTTGCGCGTAATTAAATCGTCTTAACAACCCTGGCCCGTTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGc                                                                                                          <  2:114702/150‑1 (MQ=255)
                                        ccGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACCACCCTGGCCCGGGACACATGGTACCGGGCCTCCAACGACAGCGAAACGGGGGCCGGTGACGCGGCa                                                                                                         >  2:312584/1‑150 (MQ=255)
                                        ccGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCa                                                                                                         <  1:530094/150‑1 (MQ=255)
                                        ccGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCATTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCa                                                                                                         <  2:45356/150‑1 (MQ=255)
                                             tgatgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAAcaca                                                                                                    >  1:657435/1‑150 (MQ=255)
                                              gatcGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCTGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGGAGCGGAGACACAg                                                                                                   >  2:981508/1‑150 (MQ=255)
                                               ttgGAATAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAgg                                                                                                  <  2:1099601/149‑1 (MQ=255)
                                               atgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACACCCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAGCCGTGGGCCTGAGAAGCGGCACCACAgg                                                                                                  >  1:1275511/1‑150 (MQ=255)
                                               atgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAgg                                                                                                  >  1:513135/1‑150 (MQ=255)
                                               atgGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAgg                                                                                                  <  2:531633/150‑1 (MQ=255)
                                                       aGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCAt                                                                                          <  1:1723515/150‑1 (MQ=255)
                                                          cTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACa                                                                                       >  2:1872904/1‑150 (MQ=255)
                                                              acCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTg                                                                                   <  2:776544/150‑1 (MQ=255)
                                                                ccGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGc                                                                                 <  1:1206526/150‑1 (MQ=255)
                                                                ccGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGc                                                                                 <  1:1554929/150‑1 (MQ=255)
                                                                ccGTAAACAGGTGCGTTTACTGTGCGAATGGTATGTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGc                                                                                 <  2:1298985/150‑1 (MQ=255)
                                                                      aCAGGTGCGGTTACTGTGCGAAGGGTATTTGCGCGTACGTAACTCGGCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCGAATgt                                                                           <  2:1299853/150‑1 (MQ=255)
                                                                        aGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgtag                                                                         <  2:484613/150‑1 (MQ=255)
                                                                        aGGTGCGGTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGACCGGTACACATGTTACCGGTCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATgtag                                                                         <  1:1065770/150‑1 (MQ=255)
                                                                                ttACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggggg                                                                 <  2:182342/150‑1 (MQ=255)
                                                                                ttACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggggg                                                                 >  1:1179115/1‑150 (MQ=255)
                                                                                   cTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCa                                                              >  1:1451326/1‑150 (MQ=255)
                                                                                   cTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCa                                                              >  1:718525/1‑150 (MQ=255)
                                                                                     gtgCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCata                                                            >  1:211864/1‑150 (MQ=255)
                                                                                       gCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATg                                                          >  1:1347689/1‑150 (MQ=255)
                                                                                         gAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGCCAGCGCGCCGTGGGCCGGTGAAGCGGGACCGCAGGGGGCGCAGACAGGGGGCGAAGGTAGTGCGGGGGGcgtgtggg                                                        >  2:1350480/1‑143 (MQ=255)
                                                                                         gAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGAc                                                        <  2:672867/150‑1 (MQ=255)
                                                                                           aTGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACCCCTGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCt                                                      >  2:1319526/1‑150 (MQ=255)
                                                                                           aTGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCt                                                      >  2:1264451/1‑150 (MQ=255)
                                                                                            tGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTg                                                     <  1:1810721/150‑1 (MQ=255)
                                                                                                aTTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGTGCATACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACAAAGGCGTAGCATACAGTTGGCATATGTAGTACGGGGTGCATATGACCTGGttt                                                 >  2:1965126/1‑150 (MQ=255)
                                                                                                 tttGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTa                                                <  2:606238/150‑1 (MQ=255)
                                                                                                 tttGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTa                                                >  1:1446333/1‑150 (MQ=255)
                                                                                                 tttGCCCGTACGTAAATCGGCTTAACAACCCTGGCCCTGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTa                                                <  2:93312/150‑1 (MQ=255)
                                                                                                  ttGCGCGTACGTAAATCGTCTTACCCACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTAt                                               <  1:87781/150‑1 (MQ=255)
                                                                                                    gcgcGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTg                                             <  1:696976/150‑1 (MQ=255)
                                                                                                     cgcgTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGa                                            <  2:1111343/150‑1 (MQ=255)
                                                                                                       cgcacgtaCAGCGTCTTACCTCCCCTGGCCCGGTACACAGGATCCCGGGCCTACCACGACCTCGAACCGTGGGCCTGAGAAGCGGCAACACTGGCGTATCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGAcc                                          <  1:312584/147‑1 (MQ=255)
                                                                                                           cgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTTGTACGGGGGGCCTATGACCTGGTTTATTGACCGGCg                                      >  1:112201/1‑150 (MQ=255)
                                                                                                           cgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCg                                      >  2:1695463/1‑150 (MQ=255)
                                                                                                            gtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGAGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGt                                     >  2:894900/1‑150 (MQ=255)
                                                                                                             taAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTc                                    <  2:659805/150‑1 (MQ=255)
                                                                                                               aaTCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTg                                  <  2:888399/150‑1 (MQ=255)
                                                                                                                aTCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCTTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGa                                 <  1:834511/150‑1 (MQ=255)
                                                                                                                  cGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGGTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAAc                               >  2:1017298/1‑150 (MQ=255)
                                                                                                                   gTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTTCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACg                              >  2:541284/1‑150 (MQ=255)
                                                                                                                   gTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACg                              <  1:1735289/150‑1 (MQ=255)
                                                                                                                    tCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACCGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGCACgg                             >  1:1798203/1‑150 (MQ=255)
                                                                                                                    tCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACgg                             <  1:1574348/150‑1 (MQ=255)
                                                                                                                       tAACACCCCTGGCCCGGTACACATGTTTCCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGGAAATGTAGTACGGGGGGCATATGACCGGGTTTATTGACCGGCGTCTGAACGGCaa                          >  2:631684/1‑150 (MQ=255)
                                                                                                                       tAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCTACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTTGTTTATTGACCGGCGTCTGAACGGCaa                          >  1:1082404/1‑150 (MQ=255)
                                                                                                                       tAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGGCTGAACGGCaa                          >  2:771486/1‑150 (MQ=255)
                                                                                                                         acaacCCTGCCCCGGTACACATTTAACCGGGCCACCCACGTCAGCGAACCGTGGGCCAGAGAAGCGGCAACACAGGCGTAGCAGACAGTTCGCAAATGTTGAACGGGGGGCATTTGCCCTGGTGTATTGACCGGCGTCTGAAGGGCaaaa                        >  2:1210879/1‑150 (MQ=255)
                                                                                                                           aacCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAAc                      <  1:961136/150‑1 (MQ=255)
                                                                                                                           aacCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCATGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCTGCGTCTGAACGGCAAAAAc                      <  1:97633/150‑1 (MQ=255)
                                                                                                                              ccTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACaaa                   >  1:1397543/1‑150 (MQ=255)
                                                                                                                               cTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACaaaa                  >  1:175629/1‑150 (MQ=255)
                                                                                                                                 ggCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGc                >  2:1506122/1‑150 (MQ=255)
                                                                                                                                 ggCCCGGTAACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAAAGTAGTAAGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGc                >  1:1361140/1‑150 (MQ=255)
                                                                                                                                  gCCCGGTAACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCa               >  2:709087/1‑150 (MQ=255)
                                                                                                                                   cccGGTAACATGTGTACCGGGCCTACCACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTGGGCAAATGTAGTACGGGGGGAAGATGACCTGGTTTATTGACCGGCCTCTGAACGGCAAAACCAAAAGCAt              >  2:570464/1‑150 (MQ=255)
                                                                                                                                   cccGGTAACATGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGTGCATATGACCTGGTTTATTGACCGGCGTCTGCACGGCATAAACAAAAGCAt              >  2:562602/1‑150 (MQ=255)
                                                                                                                                    ccGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACCGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGCCCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATg             >  1:255135/1‑150 (MQ=255)
                                                                                                                                    ccGGGACACATGTTACCGGGCCGACAACGACAGCGAACCGTGGGGCTGAGAAGCGGCAACACAGGCGTAGGCTACAGTTTGCAAATGGAGGAAGGGGGGCATAAGACCTGGTTTATTTACCGGCGGCAGAAAGGCAAAAAAAAAAGCATg             >  2:733495/1‑150 (MQ=255)
                                                                                                                                     cGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATgg            <  1:1358798/150‑1 (MQ=255)
                                                                                                                                     cGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATgg            <  1:577100/150‑1 (MQ=255)
                                                                                                                                      ggTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGt           <  1:502686/150‑1 (MQ=255)
                                                                                                                                       ggtaacaTGTGTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGt           >  1:1531676/5‑150 (MQ=255)
                                                                                                                                              tGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGcc   >  2:640520/1‑150 (MQ=255)
                                                                                                                                               gTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAATGCATGGTGAATCGCCa  >  1:1949581/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CGTCAACCGACGAGCAGAAGAAACACGACGACTTTGTCGACCGTATGATGGAAAAAGGCTACACCCGTAAACAGGTGCGTTTACTGTGCGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCA  >  NC_000913/1868721‑1869013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: