Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,991,805:2 +G 5.0% intergenic (+46/‑289) ygeG → / → ygeH SycD‑like chaperone family TPR‑repeat‑containing protein/putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,991,8052.G 5.0% 671.6 / 30.4 160intergenic (+46/‑289)ygeG/ygeHSycD‑like chaperone family TPR‑repeat‑containing protein/putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base . (89/63);  new base G (6/2);  total (95/65)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.09e-01

Only 100 of 167 total aligned reads displayed.

AATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTATTTCAGATTACATCACTGAATTCC  >  NC_000913/2991656‑2991954
                                                                                                                                                       |                                                                                                                                                     
aatgTTTCTATCACATTATTAAAAATTGTGAGTATGATAGTGTTAAGAGTAAAGCGCACGCATATATTGAACTCTTAAACGATATTTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTGTAGTGCAGTTAAc                                                                                                                                                         <  2:240804/150‑1 (MQ=255)
 atgTTTCTATCACATTATTAACAATTTTGAGGATGATAGTGTTAATAGTAAAGCTAAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAc‑‑c                                                                                                                                                      <  1:1221230/150‑1 (MQ=255)
 atgTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAc‑‑c                                                                                                                                                      <  1:139996/150‑1 (MQ=255)
   gTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CAc                                                                                                                                                    <  1:1057651/150‑1 (MQ=255)
          tCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCATGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAAcc                                                                                                                                             <  2:990928/150‑1 (MQ=255)
          tCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAAcc                                                                                                                                             >  1:443291/1‑150 (MQ=255)
           cacaTTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑AACTATAACCa                                                                                                                                            >  1:566991/1‑150 (MQ=255)
           aacaTTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCTCATGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTG‑‑GTTAACTGCACTATAACCa                                                                                                                                            <  2:975216/149‑1 (MQ=255)
                  ttaACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACctc                                                                                                                                     >  2:733163/1‑150 (MQ=255)
                   taACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACctct                                                                                                                                    >  1:1657706/1‑150 (MQ=255)
                    aaCAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCtt                                                                                                                                   <  1:818447/150‑1 (MQ=255)
                      cAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTAt                                                                                                                                 >  1:354809/1‑150 (MQ=255)
                       aaTTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATg                                                                                                                                >  2:1453371/1‑150 (MQ=255)
                          tgtgAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGttt                                                                                                                             >  2:922599/1‑150 (MQ=255)
                           gtgAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGtttt                                                                                                                            >  2:945531/1‑150 (MQ=255)
                            tgAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTc                                                                                                                           <  2:1805376/150‑1 (MQ=255)
                            tgAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTc                                                                                                                           >  1:1783485/1‑150 (MQ=255)
                             taGGATGATAGTGTTAAGAGTAAATCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGtttgcc                                                                                                                          <  1:294151/149‑4 (MQ=255)
                             gAGGATGATAGTGGTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCt                                                                                                                          <  2:576461/150‑1 (MQ=255)
                                 atgatAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTaaa                                                                                                                      <  1:1029374/150‑1 (MQ=255)
                                 atgatAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTaaa                                                                                                                      <  1:1376628/150‑1 (MQ=255)
                                   gatAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATc                                                                                                                    <  2:1116943/150‑1 (MQ=255)
                                    atAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCa                                                                                                                   <  1:752490/150‑1 (MQ=255)
                                             agagTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTaa                                                                                                          >  2:1027912/1‑150 (MQ=255)
                                                gTAAAGCGCAGGCATATATTGAACTCTTAATCGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGATAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAAc                                                                                                       >  1:722133/1‑150 (MQ=255)
                                                gTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAAc                                                                                                       <  2:400749/150‑1 (MQ=255)
                                                gTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAAc                                                                                                       >  2:1531338/1‑150 (MQ=255)
                                                gTAAAGCCCCGGCATATATTGAACTCTTAAACGATAATTCAGAAGATATTGGCTAAGCAATACAATAATTTGGTTAAAATTGCTGGTTATTGTGCAGTTAAC‑‑CACTATAACCCACCCCTCTTATGTTTTCTTAAATAAGCATCTTaaaaa                                                                                                       >  2:1443262/1‑149 (MQ=255)
                                                   aaGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGt                                                                                                    <  1:1544061/150‑1 (MQ=255)
                                                      cgcAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTaa                                                                                                 >  1:1338287/1‑150 (MQ=255)
                                                      cgcAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCAGTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTaa                                                                                                 >  1:292642/1‑150 (MQ=255)
                                                       gcAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAag                                                                                                >  1:176917/1‑150 (MQ=255)
                                                          ggCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGc                                                                                             >  2:419933/1‑150 (MQ=255)
                                                          ggCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGc                                                                                             >  1:1694614/1‑150 (MQ=255)
                                                                tatTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAATAGCATACta                                                                                       >  2:765157/1‑150 (MQ=255)
                                                                 atTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACtat                                                                                      <  2:1473140/150‑1 (MQ=255)
                                                                  tacaaCACTTAAACGAAAATTCAGAAGATAAAGGCTAAGCATTACAATCATTTGGTTAAATTTGATGGATATAGAGCAGTTAAC‑‑CACTATAACCAGCACCTCAAATGATTACTTAAATCAGCATCTTAAAACAGTTAAGAGCATACtata                                                                                     >  1:1616415/4‑150 (MQ=255)
                                                                       ctctTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCTCCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCa                                                                                >  2:122648/1‑150 (MQ=255)
                                                                         ctTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCatt                                                                              >  2:843360/1‑150 (MQ=255)
                                                                           tAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCattat                                                                            >  2:834563/1‑150 (MQ=255)
                                                                           tAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCattat                                                                            >  2:90139/1‑150 (MQ=255)
                                                                                  tAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCaa                                                                     <  2:1310626/150‑1 (MQ=255)
                                                                                     ttCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTTCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGc                                                                  <  2:1935489/150‑1 (MQ=255)
                                                                                     ttCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGc                                                                  >  2:546177/1‑150 (MQ=255)
                                                                                      tCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGAATCTTAAAACAGTTAAGCGCATACTATAAATCATTATTTTTCAATGct                                                                 >  1:285195/1‑149 (MQ=255)
                                                                                       cAGAAGATAATGGCTAAGAATTACAATCATTTGGTTAAATTTTCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGAACCTCTTATGTTTTCTTAAATCAGCATATTAAAACAGTTAAGATCATACTATAAATCATTATTTTTCAATGCGt                                                                >  1:659801/1‑150 (MQ=255)
                                                                                        agaagaTAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGtt                                                               <  2:1694614/150‑1 (MQ=255)
                                                                                          aagaTAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATATCCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTag                                                             <  1:657828/150‑1 (MQ=255)
                                                                                           agaTAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTaga                                                            >  1:692309/1‑150 (MQ=255)
                                                                                            taTAATTGATAAGCATTACAATCATTTGTTTAAATTTGATGGTTATAGGGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTagag                                                           <  2:1443081/149‑1 (MQ=255)
                                                                                            taTAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTagag                                                           <  2:245946/149‑1 (MQ=255)
                                                                                             aTAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAgaga                                                          <  1:1027912/150‑1 (MQ=255)
                                                                                              tAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGaa                                                         <  2:1494438/150‑1 (MQ=255)
                                                                                               aaTGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAAc                                                        <  2:1743486/150‑1 (MQ=255)
                                                                                                 tGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACaa                                                      >  2:1456174/1‑150 (MQ=255)
                                                                                                  tgCTAAGCATTTCAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑AACTATAACCAGCAACTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAAt                                                     <  2:722133/149‑1 (MQ=255)
                                                                                                  ggCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAAt                                                     >  1:1743668/1‑150 (MQ=255)
                                                                                                  ggCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAAt                                                     >  1:1087213/1‑150 (MQ=255)
                                                                                                    cTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGAGTTAGAGAACAATCt                                                   >  1:1107415/1‑150 (MQ=255)
                                                                                                     tAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTa                                                  <  1:1576026/150‑1 (MQ=255)
                                                                                                       aGCATTACAATCATTTGTTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACt                                                <  2:1614602/150‑1 (MQ=255)
                                                                                                         cATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACttt                                              <  2:1338287/150‑1 (MQ=255)
                                                                                                          aTTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTa                                             >  1:1121582/1‑150 (MQ=255)
                                                                                                          aTTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTAATTTa                                             >  2:114956/1‑150 (MQ=255)
                                                                                                           ttACAATCATTTGGTTAAATTTGCTGGTTATAGTGCATTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTAt                                            <  1:542932/150‑1 (MQ=255)
                                                                                                             aCAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCa                                          >  1:900377/1‑150 (MQ=255)
                                                                                                              cAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCaa                                         >  2:1671259/1‑150 (MQ=255)
                                                                                                              cAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCaa                                         >  2:151126/1‑150 (MQ=255)
                                                                                                              cAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCaa                                         >  1:1304824/1‑150 (MQ=255)
                                                                                                                aTCATTTGGTTAAATTTGCTTGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCaaaa                                       <  1:508771/150‑1 (MQ=255)
                                                                                                                   aTTTGGTTAAATTTGCTTGTTATAGTTCAGTTAAC‑‑AACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTg                                    <  1:22459/150‑1 (MQ=255)
                                                                                                                   aTTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTg                                    >  2:1790845/1‑150 (MQ=255)
                                                                                                                   aTTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTg                                    <  1:733163/150‑1 (MQ=255)
                                                                                                                    tttGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGa                                   <  1:823001/150‑1 (MQ=255)
                                                                                                                        gTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTtaataa                               >  2:1738683/1‑150 (MQ=255)
                                                                                                                        gTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATaa                               >  1:1669663/1‑150 (MQ=255)
                                                                                                                           ataTTTGCTGGTTATAGTGCAGTTATA‑‑CACTATAAGCAGCTCCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAATAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGATCAATCTACTTTATCAAAATTGAATaatga                            <  1:1092747/148‑4 (MQ=255)
                                                                                                                           aaaTTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGc                            <  1:596004/150‑1 (MQ=255)
                                                                                                                           aaaTTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGc                            <  2:36203/150‑1 (MQ=255)
                                                                                                                            aaTTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTAAATGCGTTAGAGAACAAAATACTTTATCAAAAATGAATAAggat                           >  2:950912/1‑148 (MQ=255)
                                                                                                                              tttGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTAt                         >  1:1592322/1‑150 (MQ=255)
                                                                                                                               gtGCTGGTTATAGTG‑‑GTTAACTGCACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTAtt                        >  1:1480721/2‑150 (MQ=39)
                                                                                                                                 gCTGGTTATAGTG‑‑GTTAACTGCACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTATTTc                      >  1:1240316/1‑150 (MQ=255)
                                                                                                                                 gCTGGTTATAGTG‑‑GTTAACTGCACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTATTTc                      >  1:955258/1‑150 (MQ=255)
                                                                                                                                  cTGGTTATAGTG‑‑GTTAACTGCACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTATTTCa                     >  1:933082/1‑150 (MQ=255)
                                                                                                                                      ttATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTATTTCAGAtt                 >  1:1174263/1‑150 (MQ=255)
                                                                                                                                        ataGTGCATGTAAC‑‑CACTATAACAAGCACCTCTTATGTTTTCTTAAATCAGCAGCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCTTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTATTTCAGATTAc               <  2:659801/150‑1 (MQ=255)
                                                                                                                                          aGTGCAGTTAAC‑‑CACTATAACAAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTATTTCAGATTACAt             <  2:808211/150‑1 (MQ=255)
                                                                                                                                           gTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTATTTCAGATTACATc            >  2:46154/1‑150 (MQ=255)
                                                                                                                                           gTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTATTTCAGATTACATc            >  1:44504/1‑150 (MQ=255)
                                                                                                                                           gTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTATTTCAGATTACATc            >  1:50669/1‑150 (MQ=255)
                                                                                                                                           gTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTATTTCAGATTACATc            >  1:589340/1‑150 (MQ=255)
                                                                                                                                              cAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTATTTCAGATTACATCACt         <  1:244504/150‑1 (MQ=255)
                                                                                                                                              cAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTATTTCAGATTACATCACt         >  2:79729/1‑150 (MQ=255)
                                                                                                                                               aGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTATTTCAGATTACATCACTg        <  1:1531338/150‑1 (MQ=255)
                                                                                                                                               aGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTATTTCAGATTACATCACTg        >  2:123955/1‑150 (MQ=255)
                                                                                                                                                gTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTATTTCAGATTACATCACTGa       >  1:457371/1‑150 (MQ=255)
                                                                                                                                                gTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGATAAGAGCATACTATAAATCAAAATTTTTCAAAGCGTTAGAGAACAATCTACTATAACAAATTTGAATTAGGAAAATACACTTTACATCAGTGa       >  2:1878292/1‑150 (MQ=255)
                                                                                                                                                     c‑‑cACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTATTTCAGATTACATCACTGAATTcc  <  2:1566388/150‑1 (MQ=255)
                                                                                                                                                     c‑‑cACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATAATATTTCAAAGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGATATTAAAGATTACATAACTCAATTca  >  1:1123851/1‑149 (MQ=255)
                                                                                                                                                       |                                                                                                                                                     
AATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTATTTCAGATTACATCACTGAATTCC  >  NC_000913/2991656‑2991954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: