Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,701,847:1 +C 6.3% intergenic (+13/+17) yhjV → / ← dppF putative transporter/dipeptide/heme ABC transporter ATPas

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,701,8471.C 6.3% 510.9 / 31.5 126intergenic (+13/+17)yhjV/dppFputative transporter/dipeptide/heme ABC transporter ATPas
Reads supporting (aligned to +/- strand):  ref base . (62/56);  new base C (2/6);  total (64/62)
Fisher's exact test for biased strand distribution p-value = 1.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.56e-01

Only 100 of 126 total aligned reads displayed.

CGTGCCTCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCGGGCGTTGAAGGCGCA  >  NC_000913/3701704‑3701992
                                                                                                                                                |                                                                                                                                                 
  tGCCTCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGt                                                                                                                                           <  1:166864/150‑1 (MQ=255)
   gCCTCATTCCGTTTTTCCTGATCTATAACGGCGCACAACCGGAAAACCTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCGGATTGAGTAAACCGGAGCGCATGGCCCCGGtt                                                                                                                                          <  2:1237179/150‑1 (MQ=255)
     cTCATTCCGTTTTTCCTGAGCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGtttt                                                                                                                                        <  1:1821350/150‑1 (MQ=255)
      tCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGGGCCATGCGCTCCGGtttac                                                                                                                                       <  2:1854040/150‑3 (MQ=255)
      tCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTtg                                                                                                                                       <  2:623862/150‑1 (MQ=255)
      tCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTGCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTtg                                                                                                                                       <  1:895398/150‑1 (MQ=255)
       cATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGCTTGAGTAAACCGGGGCCATGCGCTCCGGtttact                                                                                                                                      <  1:217656/150‑4 (MQ=255)
       cATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATGTAAAGCCTGGCTGACTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGGGCCATGCGCTCCGGtttact                                                                                                                                      <  1:1370905/150‑4 (MQ=255)
        aTTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAACCTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGGGCCATGCGCTCCGGtttactc                                                                                                                                     <  1:563675/150‑5 (MQ=255)
        aTTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTtgtg                                                                                                                                     <  2:1896336/150‑1 (MQ=255)
        aTTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCAAGTTGCTTTGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGGGCCATGCGCTCCGGtttactc                                                                                                                                     <  1:1162513/150‑5 (MQ=255)
        aTTCCGTTTTACCTGATCTATAAAGTCGCACAACTGCAAAAACTTCGCGGATTTAAAGCCTGGCGGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCAGGGCCCCCGTTTtgtg                                                                                                                                     <  1:1405602/150‑1 (MQ=255)
         ttCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGa                                                                                                                                    <  1:1488678/150‑1 (MQ=255)
            cGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGtt                                                                                                                                 >  1:1069398/1‑150 (MQ=255)
                tttCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACg                                                                                                                             >  2:593979/1‑150 (MQ=255)
                  tCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCt                                                                                                                           >  1:27570/1‑150 (MQ=255)
                   ccTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCACCGGTTTTGTGAGTTAACGCTg                                                                                                                          >  2:205022/1‑150 (MQ=255)
                    cTGATCTATAAAGTCGCTCAACTGGAAAAACTTGGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACAGGAGCGCATCGCCCCGGTTTTGTGACTTAAAGCTGc                                                                                                                         >  2:1533452/1‑150 (MQ=255)
                    cTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGc                                                                                                                         >  2:782589/1‑150 (MQ=255)
                    cTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTAAGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGc                                                                                                                         >  2:1955385/1‑150 (MQ=255)
                     tGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCGGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCg                                                                                                                        <  1:1205988/150‑1 (MQ=255)
                          tataAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGAttt                                                                                                                   <  1:1795475/150‑1 (MQ=255)
                          tataAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGAttt                                                                                                                   <  1:1803579/150‑1 (MQ=255)
                           ataAAGTCGCACAACTTGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGAtttt                                                                                                                  <  2:1540277/150‑1 (MQ=255)
                             aaaGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCa                                                                                                                >  2:191651/1‑150 (MQ=255)
                             aaaGTCGCACAACTCGAAAAACTTCGCGGATTTAAAGCCTGGCTGAATCAGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGAATGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCa                                                                                                                <  1:978097/150‑1 (MQ=255)
                                   gCACAACTGGAAAACCTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGa                                                                                                          >  1:765166/1‑150 (MQ=255)
                                   gCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGa                                                                                                          >  1:1200749/1‑150 (MQ=255)
                                   gCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGa                                                                                                          >  2:1051912/1‑150 (MQ=255)
                                   gCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGACCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGa                                                                                                          >  1:1568759/1‑150 (MQ=255)
                                     acaACTTGAAAAACTTCGCGGATGTAAAGCCTGGCGGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATc                                                                                                        <  2:1277253/150‑1 (MQ=255)
                                     acaACTGGAAAAACTTCGCGGAGTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGACGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATc                                                                                                        <  2:403699/150‑1 (MQ=255)
                                      caACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGCGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCa                                                                                                       >  2:1235393/1‑150 (MQ=255)
                                      caACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCa                                                                                                       <  2:836795/150‑1 (MQ=255)
                                           ggAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGc                                                                                                  >  1:404021/1‑150 (MQ=255)
                                              aaaaCTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCaaa                                                                                               >  1:1584979/1‑150 (MQ=255)
                                                aaCTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTATGCTATGCATGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGCGCGCATGGCCCCGGTTTTTTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAAc                                                                                             >  1:836121/1‑150 (MQ=255)
                                                     ggatgATTTAAAGCCTGTCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCt                                                                                        <  2:1701792/146‑1 (MQ=255)
                                                            ttAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTT                                                                                 >  2:1006039‑M1/1‑150 (MQ=255)
                                                              aaaGCCTGTCTGATTCTGCTGTACGGCATTTTGCTATGCTGGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCACGGTTTTGTGATTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGa                                                                               <  1:576830/150‑1 (MQ=255)
                                                              aaaGCCTGGCTGCTTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGa                                                                               <  1:568865/150‑1 (MQ=255)
                                                                aGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGAcc                                                                             >  1:1844187/1‑150 (MQ=255)
                                                                 gCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGAccg                                                                            >  2:1505081/1‑150 (MQ=255)
                                                                  ccTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGAccgc                                                                           <  1:191221/150‑1 (MQ=255)
                                                                   cTGGCTGATTATTCTGTACGGCATTTTGATATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAAACGGAGCGCATTGCCCCGTTTTTGTGAGTTAACGCTTCGGATTTTCATCCTGATCAACAGCAAAACTAGCTACCAGTTGAccgcc                                                                          <  1:304038/150‑1 (MQ=255)
                                                                     ggCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGt                                                                        >  1:1427369/1‑150 (MQ=255)
                                                                     ggCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCAGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGt                                                                        <  2:1007794/150‑1 (MQ=255)
                                                                           ttCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCtt                                                                  <  1:1322343/150‑1 (MQ=255)
                                                                             ctgctgTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCtttt                                                                <  1:1663517/150‑1 (MQ=255)
                                                                              tgctgTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGTCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTa                                                               <  1:1020328/150‑1 (MQ=255)
                                                                               gctgTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAg                                                              >  2:684392/1‑150 (MQ=255)
                                                                                ctgTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGc                                                             <  2:1329746/150‑1 (MQ=255)
                                                                                  gTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTg                                                           >  2:27890/1‑150 (MQ=255)
                                                                                    aCGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCg                                                         >  2:220711/1‑150 (MQ=255)
                                                                                    aCGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCg                                                         >  1:1191463/1‑150 (MQ=255)
                                                                                    aCGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCg                                                         >  2:1321237/1‑150 (MQ=255)
                                                                                     cGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGCCCGCCGTAGTCTTTTAGCTGCgg                                                        >  1:383812/1‑150 (MQ=255)
                                                                                        cATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGaccgccgcagctaaaagactacggcgg                                                     <  1:761967‑M1/150‑28 (MQ=255)
                                                                                        cATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGaccgccgcagctaaaagactacggcgg                                                     <  2:1163836‑M1/150‑28 (MQ=255)
                                                                                        cATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTg                                                     <  1:858799/150‑1 (MQ=255)
                                                                                         aTTTTTCAATGCTTTTCGCCACTGTTGAAGCTGATTGTGTAACCCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACTCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGc                                                    >  2:374478‑M2/1‑149 (MQ=255)
                                                                                         aTTTTGCTATGCTTGTCGCCACTGTTGACGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGc                                                    >  1:167201‑M2/1‑149 (MQ=255)
                                                                                         aTTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGc                                                    <  2:18826‑M2/150‑2 (MQ=255)
                                                                                         aTTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGc                                                    <  2:791139‑M2/150‑2 (MQ=255)
                                                                                            ttGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGcaac                                                 >  1:1515624‑M2/1‑146 (MQ=255)
                                                                                              gCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGcaactg                                               <  2:385757‑M2/150‑7 (MQ=255)
                                                                                              gCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGcaactg                                               <  1:35731‑M2/150‑7 (MQ=255)
                                                                                               cTATGCTTGTCGCCACTGTTGAAGCTGATTTAGTAAACCGGAGCGCATTGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCATGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGcaactcg                                              <  1:266974‑M2/150‑8 (MQ=255)
                                                                                               cTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGcaactgg                                              <  2:464084‑M2/150‑8 (MQ=255)
                                                                                                tATGCTTGGCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTggg                                             <  2:85290/150‑1 (MQ=255)
                                                                                                 aTGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGt                                            >  2:1045718/1‑150 (MQ=255)
                                                                                                  tGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTg                                           >  2:1157822/1‑150 (MQ=255)
                                                                                                  tGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTg                                           >  1:896548/1‑150 (MQ=255)
                                                                                                  tGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTg                                           >  1:895684/1‑150 (MQ=255)
                                                                                                  tGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTg                                           <  2:1974720/150‑1 (MQ=255)
                                                                                                  tGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTg                                           >  1:759110/1‑150 (MQ=255)
                                                                                                   gCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGc                                          >  1:1642769/1‑150 (MQ=255)
                                                                                                         cGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCGGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGc                                    <  1:956700/150‑1 (MQ=255)
                                                                                                           ccACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCg                                  >  1:1194457/1‑150 (MQ=255)
                                                                                                              cTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAg                               <  2:1369406/150‑1 (MQ=255)
                                                                                                                gTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAgcg                             >  2:414407/1‑150 (MQ=255)
                                                                                                                     aGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCcgac                        >  2:948003/1‑150 (MQ=255)
                                                                                                                     aGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGAAGGGGCCGAAGCGCcgac                        >  1:848351/1‑150 (MQ=255)
                                                                                                                      gCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCcgacg                       >  1:1598483/1‑150 (MQ=255)
                                                                                                                        tGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCgacgac                     >  1:413494/1‑150 (MQ=255)
                                                                                                                         gATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCCACTGGGTGCAGGGGCCGAAGCGCCgacgacc                    >  1:778581/1‑149 (MQ=255)
                                                                                                                         gATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACa                    >  2:909872/1‑150 (MQ=255)
                                                                                                                            tGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCg                 >  1:1949407/1‑150 (MQ=255)
                                                                                                                             gAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCgg                >  1:62740/1‑150 (MQ=255)
                                                                                                                             cacaaaaCCGGGGCCATGCGCTCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCgg                >  2:1269897/5‑150 (MQ=255)
                                                                                                                              aGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCggg               >  2:1490128/1‑150 (MQ=255)
                                                                                                                               caaaaCCGGGGCCATGCGCTCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTTGGTGCAGGGGCCGAAGCGCCGACGACAGCGGGc              >  1:1798814/3‑150 (MQ=255)
                                                                                                                                tAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGCAGCGCCGCCGACAGCGGGCg             >  2:1489296/1‑150 (MQ=255)
                                                                                                                                tAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCGGGCg             >  1:816063/1‑150 (MQ=255)
                                                                                                                                tAAACCGGAGCGCACGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCGGGCg             <  2:1355734/150‑1 (MQ=255)
                                                                                                                                  aaCCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGCCCGCCGTATTCTTTTAGCTGCGGCAGCAACTGGGTGAAGGGGGCGAATCGCCGACGACAGCGGACgat           >  2:152221/1‑148 (MQ=255)
                                                                                                                                  aaCCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCGGGCGtt           >  2:1442164/1‑150 (MQ=255)
                                                                                                                                   aCCGGCGCGCATGGCCCCGGTTTTGTGAGATAACGCTGCGGATATTCATCCTGATCAACAGCAACACACGCTACCAGTTGACCGCCGTAGTCTATTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCGGGCGTTg          <  2:957736/150‑1 (MQ=255)
                                                                                                                                    ccGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCGGGCGTTGa         >  2:1841104/1‑150 (MQ=255)
                                                                                                                                           gcATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCGGGCGTTGAAGGCGCa  <  2:1844187/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
CGTGCCTCATTCCGTTTTTCCTGATCTATAAAGTCGCACAACTGGAAAAACTTCGCGGATTTAAAGCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCGCCGACGACAGCGGGCGTTGAAGGCGCA  >  NC_000913/3701704‑3701992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: