Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 975,701 A→C 6.5% D27A (GAC→GCC) ‡ mukE → chromosome condensin MukBEF, MukE localization factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913975,7010AC 6.5% 341.5 / 10.1 118D27A (GAC→GCC) ‡mukEchromosome condensin MukBEF, MukE localization factor
Reads supporting (aligned to +/- strand):  ref base A (50/60);  new base C (4/4);  total (54/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

Only 100 of 120 total aligned reads displayed.

CAAGCAGATTTCACCGGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAGGGTTCTTCTATTTACGCC  >  NC_000913/975552‑975850
                                                                                                                                                     |                                                                                                                                                     
cAAGCAGATTTCACCGGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGa                                                                                                                                                       <  2:1798542/150‑1 (MQ=255)
 aaGCAGAATTCACCCGACTGCCCGCGAAATGGCAGCCGATTAATGATTACGGAGCCACGGTACAGGCGCATGACATCGACAAATATTGAACAAGTGATGCCGGTTAAGCGGGCGCAGGCGCTGGCGAATCCGTTATTACCGGCGCTGGAc                                                                                                                                                      <  2:78731/150‑1 (MQ=255)
  aGCAGATTTCACCGGACTGCCAGCGAAATAGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTACGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGAcc                                                                                                                                                     >  1:1598390/1‑149 (MQ=255)
    cAGATTTCACCGGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAgc                                                                                                                                                   >  2:1401159/1‑150 (MQ=255)
    cAGATTTCACCGGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAgc                                                                                                                                                   <  1:732549/150‑1 (MQ=255)
        tttCACCGGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCt                                                                                                                                               >  1:1924556/1‑150 (MQ=255)
         ttCACCGGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGTCCAGCGccgg                                                                                                                                              <  2:442271/150‑3 (MQ=255)
         ttCACCGGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGTCCAGCGccgg                                                                                                                                              <  2:747495/150‑3 (MQ=255)
         ttCACCGGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGTCCAACGccgg                                                                                                                                              <  1:1560988/150‑3 (MQ=255)
          tCACCGGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGGCTAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAAGCCATTATTTCCGGCGCTGGACAGCGCCtgg                                                                                                                                             >  1:342774/1‑148 (MQ=255)
           cACCGGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTAc                                                                                                                                            >  1:143492/1‑150 (MQ=255)
             ccGGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGt                                                                                                                                          <  1:534357/150‑1 (MQ=255)
             ccGGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCAGTTATTTCCGGCGCTGGACAGCGCCTTACGt                                                                                                                                          >  1:39963/1‑150 (MQ=255)
                gACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCa                                                                                                                                       <  1:1389598/150‑1 (MQ=255)
                    gCCAGCGAAATGGCAGCCGATTAATGATTAGGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGAc                                                                                                                                   <  1:65147/150‑1 (MQ=255)
                    gCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGAc                                                                                                                                   <  1:615492/150‑1 (MQ=255)
                     ccAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACg                                                                                                                                  >  2:1480364/1‑150 (MQ=255)
                     ccAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACg                                                                                                                                  >  2:347200/1‑150 (MQ=255)
                      cAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGc                                                                                                                                 >  1:642760/1‑150 (MQ=255)
                       aGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCTTTACGTTCAGGACGcc                                                                                                                                <  2:100686/150‑1 (MQ=255)
                        gCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACACGTGATGCCGGTTAAGCGGGCGCCGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCGTACGTTCAGGACGCCa                                                                                                                               <  2:854946/150‑1 (MQ=255)
                        gCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCa                                                                                                                               <  2:854943/150‑1 (MQ=255)
                        gCGAAATGGCAGCAGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCAGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCa                                                                                                                               >  2:1804127/1‑150 (MQ=255)
                                  aGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAATTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGCCGCCATATTTGCCTc                                                                                                                     >  1:355515/1‑150 (MQ=255)
                                   gCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTcg                                                                                                                    >  2:1476424/1‑150 (MQ=255)
                                      gATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGACCAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTcgacg                                                                                                                 <  2:1230143/150‑1 (MQ=255)
                                      gATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTcgacg                                                                                                                 <  2:30773/150‑1 (MQ=255)
                                       aTTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCGGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTcgacga                                                                                                                <  2:1248995/150‑1 (MQ=255)
                                          aaTGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACt                                                                                                             >  2:897071/1‑150 (MQ=255)
                                          aaTGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACt                                                                                                             <  1:525315/150‑1 (MQ=255)
                                           aTGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGTACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTg                                                                                                            <  1:94497/150‑1 (MQ=255)
                                           aTGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTg                                                                                                            >  1:1878556/1‑150 (MQ=255)
                                            tGATTACGGAGCCAATGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTgg                                                                                                           <  2:1987614/150‑1 (MQ=255)
                                            tGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCTCAGGCGCTGGCGAATCCGTTATTTCCGTCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGACTCGACGAACTgg                                                                                                           >  1:1237855/1‑150 (MQ=255)
                                            tGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGAGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTgg                                                                                                           >  1:1971154/1‑150 (MQ=255)
                                              aTTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGAt                                                                                                         >  2:45554/1‑150 (MQ=255)
                                               ttACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATa                                                                                                        >  1:927601/1‑150 (MQ=255)
                                                 aCGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAAt                                                                                                      <  2:1678442/150‑1 (MQ=255)
                                                 aCGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAAt                                                                                                      >  1:1525124/1‑150 (MQ=255)
                                                  cGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATc                                                                                                     <  2:753509/150‑1 (MQ=255)
                                                  cGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATc                                                                                                     <  2:1742765/150‑1 (MQ=255)
                                                    gAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCAt                                                                                                   <  2:143492/150‑1 (MQ=255)
                                                     aGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATg                                                                                                  <  1:1601222/150‑1 (MQ=255)
                                                        cAAGGTACAGGCGCTGGGCATCGACAAATATTGAACTAGTGAGGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCAt                                                                                               <  1:1380966/150‑1 (MQ=255)
                                                           ggTACAGGCGCATGTCATCGACAAATATTGAACAAGTGACGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTcc                                                                                            >  2:629979/1‑150 (MQ=255)
                                                            gTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCt                                                                                           <  2:279806/150‑1 (MQ=255)
                                                              aCAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGa                                                                                         >  1:1844658/1‑150 (MQ=255)
                                                               cAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGAt                                                                                        >  1:1352480/1‑150 (MQ=255)
                                                               cAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGAt                                                                                        <  1:385860/150‑1 (MQ=255)
                                                                aGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATg                                                                                       <  1:1136607/150‑1 (MQ=255)
                                                                aGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATg                                                                                       <  2:1932768/150‑1 (MQ=255)
                                                                aGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATg                                                                                       <  2:1782431/150‑1 (MQ=255)
                                                                  gcgcATGTCATCGACAAATATTGAACACGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGCCAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGa                                                                                     <  2:1410746/150‑1 (MQ=255)
                                                                         tCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAg                                                                              >  2:776249/1‑150 (MQ=255)
                                                                           aTCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGa                                                                            >  2:895644/1‑150 (MQ=255)
                                                                             cGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAat                                                                          <  1:896967/150‑1 (MQ=255)
                                                                             cGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAat                                                                          >  1:466343/1‑150 (MQ=255)
                                                                              gACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAata                                                                         <  1:59317/150‑1 (MQ=255)
                                                                                cAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATc                                                                       >  2:481829/1‑150 (MQ=255)
                                                                                cAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATc                                                                       <  1:1861135/150‑1 (MQ=255)
                                                                                cAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATc                                                                       >  2:901483/1‑150 (MQ=255)
                                                                                  aaTATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTg                                                                     <  1:1990207/150‑1 (MQ=255)
                                                                                      ttGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATATTCATGCATTCCTGATGGATTTTCAGGAATATCTGGAag                                                                 >  2:195791/1‑150 (MQ=255)
                                                                                        gAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAagag                                                               >  2:726409/1‑150 (MQ=255)
                                                                                        gAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCAGATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAagag                                                               >  1:478188/1‑150 (MQ=255)
                                                                                        gAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACACCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAagag                                                               >  1:1947445/1‑150 (MQ=255)
                                                                                         aaCAAGTGCTGCCGGTTAAGCTGGCGCAGGCGCGGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGt                                                              <  1:1259721/150‑1 (MQ=255)
                                                                                          aCAAGTGATGACGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGtt                                                             <  2:1191530/150‑1 (MQ=255)
                                                                                              gTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTAc                                                         <  2:1271017/150‑1 (MQ=255)
                                                                                                 aTGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTAcgcg                                                      <  2:909572/150‑1 (MQ=255)
                                                                                                  tGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTAcgcgc                                                     >  2:1166662/1‑150 (MQ=255)
                                                                                                   gCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTAcgcgcg                                                    <  1:1112191/150‑1 (MQ=255)
                                                                                                     cGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGTATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGATGAGTTTTACGCGCGtt                                                  <  1:233869/150‑1 (MQ=255)
                                                                                                          aaGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAAc                                             <  1:1716429/150‑1 (MQ=255)
                                                                                                           aGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACg                                            >  1:284200/1‑150 (MQ=255)
                                                                                                           aGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACg                                            >  1:676498/1‑150 (MQ=255)
                                                                                                            tcTGGCGCAGGCGCAGGCGAAACCGTTATTTCAGGCGCTGGACAGCGCCTTACTTTCACGACGCCATATTGGCCTCCACGAACTGGATAATCATGCATTCCTGATGGATATTCAGGAATATCTGGAAGAGATTTACGCGAGTAATAACGt                                           >  2:739406/2‑150 (MQ=255)
                                                                                                            gCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGt                                           <  1:347200/150‑1 (MQ=255)
                                                                                                                  gcAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCt                                     <  1:1267627/150‑1 (MQ=255)
                                                                                                                       cgcTGGCGAATCCGTTATTTCCGGCGCTTGACAGCGCCTTACGTTCAGGACGCCAAAATGGCCTCGACGAACGGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTc                                >  2:845707/1‑150 (MQ=255)
                                                                                                                       cgcTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTc                                <  1:995098/150‑1 (MQ=255)
                                                                                                                        gcggtcGAATCCGCTATTTCCCGCGCTGGACAGCGGCTTACGTTCCGTACCCACTCTAGGCCTCGACGAACTGTATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGATGAGTTTTACGCGCGTTATAACGTTGAGCTTATTcg                               <  2:384444/145‑1 (MQ=255)
                                                                                                                           ggCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTAgcgc                            >  2:434753/1‑150 (MQ=255)
                                                                                                                            gCGAATCCGTTATTTCGGGCGCTGGACAGCGCCTTAGGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTACTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCa                           <  2:1237855/150‑1 (MQ=255)
                                                                                                                               aaTCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCa                        >  1:822647/1‑150 (MQ=255)
                                                                                                                                   cGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAg                    <  2:953362/150‑1 (MQ=255)
                                                                                                                                   cGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAg                    <  2:1143276/150‑1 (MQ=255)
                                                                                                                                     ttATTTCCTGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAggg                  <  1:1401159/150‑1 (MQ=255)
                                                                                                                                       acgtaaggCGCTGTCCAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAGGGtt                >  2:1194683/7‑150 (MQ=255)
                                                                                                                                         ttCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAGGGttct              <  1:726409/150‑1 (MQ=255)
                                                                                                                                         gtaaggCGCTGTCCAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAGGGttct              >  2:1643471/5‑150 (MQ=255)
                                                                                                                                          taaggCGCTGTCCAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAGGGttctt             >  1:236620/4‑150 (MQ=255)
                                                                                                                                          tCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAGGGttctt             >  2:1718789/1‑150 (MQ=255)
                                                                                                                                           ccGGCGCTGGACAGCGCCTTACGTTCAGGTCGCCATATTGGCCTCGACGAACTGGATAATCATTCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAGGGttcttc            <  1:45554/150‑1 (MQ=255)
                                                                                                                                           ccGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAGGGtgcttg            >  2:1246162/1‑149 (MQ=255)
                                                                                                                                             ggCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGTTTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAGGGTTCTTCTa          <  2:1860535/150‑1 (MQ=255)
                                                                                                                                                gcTGGACAGCGCCTTACGTGCAGGACGCCATATTGGCCTCGCCGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAGGGTTCTTCTAttt       <  1:740487/150‑1 (MQ=255)
                                                                                                                                                gcTGGACAGCGCCTTACGATCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAGGGTTCTTCTAttt       <  1:730185/150‑1 (MQ=255)
                                                                                                                                                   ggACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAGGGTTCTTCTATTTACg    >  2:1147103/1‑150 (MQ=255)
                                                                                                                                                     aCAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAGGGTTCTTCTATTTACGcc  <  2:1616625/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAAGCAGATTTCACCGGACTGCCAGCGAAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGCGTTATAACGTTGAGCTTATTCGCGCACCAGAAGGGTTCTTCTATTTACGCC  >  NC_000913/975552‑975850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: