Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 980,800 G→T 5.7% intergenic (+14/‑247) mukB → / → ldtD chromosome condensin MukBEF, ATPase and DNA‑binding subunit/murein L,D‑transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913980,8000GT 5.7% 381.1 / 6.9 121intergenic (+14/‑247)mukB/ldtDchromosome condensin MukBEF, ATPase and DNA‑binding subunit/murein L,D‑transpeptidase
Reads supporting (aligned to +/- strand):  ref base G (57/56);  new base T (3/4);  total (61/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

AGGCACCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAACGTATCGGCGTTTATATACTGAAGATAAGCCTGATGAGTAACAGG  >  NC_000913/980651‑980949
                                                                                                                                                     |                                                                                                                                                     
aGGCACCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGgcag                                                                                                                                                       <  2:1129435/150‑1 (MQ=255)
  gCACCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGgcagca                                                                                                                                                     >  1:1112358/1‑150 (MQ=255)
  gCACCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGgcagca                                                                                                                                                     >  1:1238370/1‑150 (MQ=255)
  gCACCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGgcagca                                                                                                                                                     <  2:551539/150‑1 (MQ=255)
   caccacCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCaa                                                                                                                                                    >  2:1620092/1‑150 (MQ=255)
    accaccAATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAAt                                                                                                                                                   <  2:2000225/150‑1 (MQ=255)
      caccTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATgc                                                                                                                                                 <  1:1969637/150‑1 (MQ=255)
          tataAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATgccgcc                                                                                                                                             <  2:1469719/150‑1 (MQ=255)
          tataAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATgccgcc                                                                                                                                             >  2:629991/1‑150 (MQ=255)
           ataAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCct                                                                                                                                            <  2:889312/150‑1 (MQ=255)
             aaaCTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCcttt                                                                                                                                          <  1:985691/150‑1 (MQ=255)
              aaCTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGACGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCctttt                                                                                                                                         >  1:334911/1‑150 (MQ=255)
               aCTGGTGCGTAAAGGCTTCCAGAATACCGAACACGATCATGTCGTCGGCCTGCGATGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCATTGCTGCCGCcttaat                                                                                                                                        <  2:1635282/150‑4 (MQ=255)
                      cGTAAAGTCTTCCAGAATACCGAACACGTTCTTGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTcc                                                                                                                                 <  2:236659/150‑1 (MQ=255)
                      cGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGAGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTcc                                                                                                                                 >  1:1773836/1‑150 (MQ=255)
                      cGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCACGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTcc                                                                                                                                 <  1:866580/150‑1 (MQ=255)
                       gTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCg                                                                                                                                <  2:730655/150‑1 (MQ=255)
                       gTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCg                                                                                                                                >  2:1062530/1‑150 (MQ=255)
                       gTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTCGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCg                                                                                                                                <  1:927589/150‑1 (MQ=255)
                            gTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAAc                                                                                                                           >  1:1394811/1‑150 (MQ=255)
                            gTCTTCCAGAATACCGAACACGTGCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCACGAACTGACGAAGGGCCTTCTGAGGCGAGTTAAAATTAAGCCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAAc                                                                                                                           <  1:1737191/150‑1 (MQ=255)
                                  cAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGTATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAACAATGCCGCCTTTTCTTTATCCGAAAACTCCGtt                                                                                                                     >  2:786111/1‑150 (MQ=255)
                                  cAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGtt                                                                                                                     <  2:1808360/150‑1 (MQ=255)
                                  cAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGtt                                                                                                                     >  1:1162554/1‑150 (MQ=255)
                                     aaTACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCt                                                                                                                  <  2:1777075/150‑1 (MQ=255)
                                      aTACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGGCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTg                                                                                                                 <  2:97994/150‑1 (MQ=255)
                                          cGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACt                                                                                                             >  2:1938099/1‑150 (MQ=255)
                                          cGAACACGTTCATGTCGGCGGCCTGCGATGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAATCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACt                                                                                                             <  2:1353299/150‑1 (MQ=255)
                                             acacGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTaaa                                                                                                          >  1:1882352/1‑150 (MQ=255)
                                                  ttCATGTCGTCGGCCTGCGAGGATTTGCGCCGCACCTCCCTGAAGCGCGTCCAGGAACTGCCGAAGCGCCTTCTCAGGCGAGTTAAAATTACGGCGGCAGCCATGCCGGCTTGTCTTTTTCCGAAAACTCCGTTGCTGCACTAAAAAAGt                                                                                                     >  2:62220/1‑150 (MQ=255)
                                                     aTGTCGTCGGCCTGCGAGGTTTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGc                                                                                                  <  1:379768/150‑1 (MQ=255)
                                                     aTGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGc                                                                                                  <  2:1797266/150‑1 (MQ=255)
                                                     aTGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGc                                                                                                  <  2:433871/150‑1 (MQ=255)
                                                     aTGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGc                                                                                                  <  2:1805281/150‑1 (MQ=255)
                                                       gtcgtcGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGcac                                                                                                >  2:1462624/1‑150 (MQ=255)
                                                       gtcgtcGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGcac                                                                                                >  2:347137/1‑150 (MQ=255)
                                                           tcgGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTa                                                                                            >  1:1486846/1‑150 (MQ=255)
                                                            gggCCTGCGAGGATTTGCGCCGCAACTCCCTAACACGCTTCCAGGAACTGTCGAAGCGCGTTCTCAGGCGAGTTAATATTAAGGCGGCAGCTATTCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTAc                                                                                           <  2:262635/149‑1 (MQ=255)
                                                              gCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACgg                                                                                         >  2:410038/1‑150 (MQ=255)
                                                               ccTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGgc                                                                                        >  1:1320106/1‑150 (MQ=255)
                                                                   cGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAATCTCCGTTTCTGCACTAAAAAAGTGGCACATTACGgcgcgg                                                                                    >  2:934052/1‑149 (MQ=255)
                                                                    gAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCt                                                                                   <  1:1063981/150‑1 (MQ=255)
                                                                        ttttGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTc                                                                               <  2:889378/149‑1 (MQ=255)
                                                                        aTTTGCGCCGCAACTCCCTGAAACGCATCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTACGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTc                                                                               <  1:1414047/150‑1 (MQ=255)
                                                                          ttGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCtt                                                                             <  1:1968585/150‑1 (MQ=255)
                                                                           tgggccgcAACTACCTGAAACGCTTCCATGAACTGACGAAGCGCGTTCGCATGCGATTTAAAATTAAGGCGGGAGCAATGGCGCCTTTTCTTTTTACTAAAACTCCGTTTCTGCACTAAAATAGTGGCACATTACGGCGCtcttttcttt                                                                            <  2:1295654/147‑1 (MQ=255)
                                                                             cgctgcAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTaa                                                                          >  2:581689/1‑150 (MQ=255)
                                                                             cgccgcAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTaa                                                                          >  2:447050/1‑150 (MQ=255)
                                                                              gccgcAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTaaa                                                                         <  2:471279/150‑1 (MQ=255)
                                                                              gccgcAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTaaa                                                                         <  1:1465324/150‑1 (MQ=255)
                                                                              gccgcAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTaaa                                                                         <  1:520775/150‑1 (MQ=255)
                                                                              gccgcAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTaaa                                                                         <  1:751036/150‑1 (MQ=255)
                                                                               ccgcAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAAc                                                                        >  1:1375533/1‑150 (MQ=255)
                                                                                     cTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGGTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCttt                                                                  <  1:737445/150‑1 (MQ=255)
                                                                                      tCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTa                                                                 <  1:507249/150‑1 (MQ=255)
                                                                                      tCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTa                                                                 >  2:336479/1‑150 (MQ=255)
                                                                                            aaaCGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAg                                                           <  2:751352/150‑1 (MQ=255)
                                                                                              aCGCTTCCAGGAACTGACGAAGCGCCTTCTCATGCGAGTTAAAATTAAGGCGGAAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCAAATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGt                                                         <  1:1910041/150‑1 (MQ=255)
                                                                                              aCGCCTCCCGGAACTGACGAAGCGCCTTCTCAGGCGTGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGt                                                         <  2:98009/150‑1 (MQ=255)
                                                                                                gCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCATCAATGCCGCATTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTGTTCTTTAAACTTCTTTACATTAGGTTa                                                       <  1:786111/150‑1 (MQ=255)
                                                                                                gCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTa                                                       >  1:691969/1‑150 (MQ=255)
                                                                                                 cTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTAt                                                      >  2:1136/1‑150 (MQ=255)
                                                                                                    ccAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTa                                                   >  1:1072071/1‑150 (MQ=255)
                                                                                                     cAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCTCCTTTTCTTTTTACGAAAAATCCGTTTCTTCACTAAAAAAGTGGCTCATTACGGCGCGCTTTTCTTTAAACTTCTTTAAATTAGGTTATGTaa                                                  >  2:1201003/1‑150 (MQ=255)
                                                                                                     cAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTaa                                                  >  1:653271/1‑150 (MQ=255)
                                                                                                     cAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTaa                                                  >  2:828961/1‑150 (MQ=255)
                                                                                                     cAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTaa                                                  >  1:767855/1‑150 (MQ=255)
                                                                                                     cAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTaa                                                  >  1:849839/1‑150 (MQ=255)
                                                                                                         aaCTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAAc                                              <  1:255087/150‑1 (MQ=255)
                                                                                                         aaCTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAAc                                              >  2:1520873/1‑150 (MQ=255)
                                                                                                          aCTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAACg                                             >  1:1026797/1‑150 (MQ=255)
                                                                                                           cTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAACGt                                            >  1:1240275/1‑150 (MQ=255)
                                                                                                            tGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAACGTa                                           >  1:683249/1‑150 (MQ=255)
                                                                                                                gAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAACGTATCgg                                       >  1:898260/1‑150 (MQ=255)
                                                                                                                gAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAACGTATCgg                                       >  1:452683/1‑150 (MQ=255)
                                                                                                                    cgcCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAACGTATCGGCGtt                                   <  2:1240275/150‑1 (MQ=255)
                                                                                                                      ccTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAACGTATCGGCGTTta                                 >  1:294299/1‑150 (MQ=255)
                                                                                                                      ccTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCGTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAATAAAGTGGCACATTACGGCGCGCTTTTCTTTATACTTCTTTACATTAGGGTATGTAAAAAGGTATCGGCGTTta                                 >  1:1016041/1‑150 (MQ=255)
                                                                                                                        ttCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCCTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAACGTATCGGCGTTtatt                               >  1:393756/1‑149 (MQ=255)
                                                                                                                        tgagaaGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAACGTATCGGCGTTtata                               >  2:735903/6‑150 (MQ=255)
                                                                                                                           tcAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCACTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAACGTATCGGCGTTTATATAc                            <  2:560986/150‑1 (MQ=255)
                                                                                                                            cAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAACGTATCGGCGTTTATATACt                           >  1:55409/1‑150 (MQ=255)
                                                                                                                            cAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATCAGGTTATGTAAAAACGTATCGGCGTTTATATACt                           >  1:583873/1‑150 (MQ=255)
                                                                                                                              ggCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAACGTATCGGCGTTTATATACTGa                         <  2:524693/150‑1 (MQ=255)
                                                                                                                                cGAGTTAAAATTACGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACACCGTTTCTGCACTAAAAAAGGGGCACATTACGGCGCGCTTTTCTTTAAACTTCTATACATTAGGTTATGTAAAACCGTATCGGCGTTTATATACTGAAg                       <  2:1084542/150‑1 (MQ=255)
                                                                                                                                cGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAACGTATCGGCGTTTATATACTGAAg                       >  1:673605/1‑150 (MQ=255)
                                                                                                                                cGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCCCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAACGTATCGGCGTTTATATACTGAAg                       >  2:1414550/1‑150 (MQ=255)
                                                                                                                                   gTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAACGTATCGGCGTTTATATACTGAAGATa                    >  2:352088/1‑150 (MQ=255)
                                                                                                                                     tAAAATTAAGGCGGCAGCAATGCCGCCTTTTCGTTTTCCGAAAACTCCGTTTCTGCCCTAAAAAAGTGGAACATTACGGCGCGCTTATCTTTAAACTTCTTAACATTAGGTTATGAAAAAACGTATCGGCGATTATATACTTAAGATAAg                  >  1:144648/1‑150 (MQ=255)
                                                                                                                                       aagaaaaGGCGGCATTGCTGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGCTTATGTAAAAACGTATCGGCCTTTATATACTGAAGATAATcc                >  2:1350248/6‑150 (MQ=255)
                                                                                                                                       aaaTTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAACGTATCGGCGTTTATATACTGAAGATAAGcc                >  2:1175280/1‑150 (MQ=255)
                                                                                                                                       aaaTTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGAACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAACGTATCGGCGTTTATATACTTAAGATAAGcc                >  2:55426/1‑150 (MQ=255)
                                                                                                                                        aaTTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAACGTATCGGCGTTTATATACTGAAGATAAGCCt               <  2:529080/150‑1 (MQ=255)
                                                                                                                                        aaTTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAACGTATCGGCGTTTATATACTGAAGATAAGCCt               >  1:562287/1‑150 (MQ=255)
                                                                                                                                        aaTTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAACGTATCGGCGTTTATATACTGAAGATAAGCCt               >  2:1596239/1‑150 (MQ=255)
                                                                                                                                           tAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAACGTATCGGCGTTTATATACTGAAGATAAGCCtgat            <  1:1620092/150‑1 (MQ=255)
                                                                                                                                           aaaGGCGGCATTGCTGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAACGTATCGGCGTTTATATACTGAAGATAAGCCtgat            >  2:1629156/2‑150 (MQ=255)
                                                                                                                                            aaGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTCTACATTAGGTTATGTAAAAACGTATCGGCGTTTATATACTGAAGATAAGCCtgatg           <  1:1508275/150‑1 (MQ=255)
                                                                                                                                                cggcAGCAATGCCGCCTTTTCTTTTTACGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAACGTATCGGCGTTTATATACTGAATATAAGCATTATGATTa       >  1:1651041/1‑150 (MQ=255)
                                                                                                                                                     gcaATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGAGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTATAAACGGATCGGCGGTTATATACTGAAGATAAGCCTGATGAGTAGCAgg  >  2:734719/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGGCACCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATTAGGTTATGTAAAAACGTATCGGCGTTTATATACTGAAGATAAGCCTGATGAGTAACAGG  >  NC_000913/980651‑980949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: