Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 998,450 T→C 5.9% intergenic (+43/‑40) elfA → / → elfD laminin‑binding fimbrin subunit/putative periplasmic pilin chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913998,4500TC 5.9% 452.1 / 12.1 154intergenic (+43/‑40)elfA/elfDlaminin‑binding fimbrin subunit/putative periplasmic pilin chaperone
Reads supporting (aligned to +/- strand):  ref base T (67/77);  new base C (3/6);  total (70/84)
Fisher's exact test for biased strand distribution p-value = 5.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.47e-01

Only 100 of 154 total aligned reads displayed.

ATGGAGATCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGACTTGCAG  >  NC_000913/998304‑998595
                                                                                                                                                  |                                                                                                                                                 
aTGGAGATCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCt                                                                                                                                                <  1:212312/150‑1 (MQ=255)
 tGGAGATCATACGTTGAGTTTTAAATTTGATTATATCGCTACGGTGAAAGGGACATCAGGTAATGTGAATGCGGAAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTATTATTAATTAGTACCCCTCCAGTGTTCTg                                                                                                                                               <  2:359141/150‑1 (MQ=255)
 tGGAGATCAGACGTTTACTTTTAAAGTTTATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCAAATGTTCTg                                                                                                                                               >  2:1165447/1‑150 (MQ=255)
 tGGAGAGCAGACGTTGAATTATAAAGTTCCTTATATCGCTACGGGGAAAGCGACTTCAGCTAATGTGAATGCGGTAACAAATTTCCATATAAACTATTAATATTTGTACTCATTAATTGTTTTATTTATTAGTACCCCTCCAGTGTTCTg                                                                                                                                               <  2:1222593/150‑1 (MQ=255)
  ggAGATCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACACCTCCAGTGTTCTgg                                                                                                                                              >  2:1093054/1‑150 (MQ=255)
     gaTCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCTGTAACAAATTTCCATATTAACTTTTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAgg                                                                                                                                           <  1:1093925/150‑1 (MQ=255)
     gaTCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAgg                                                                                                                                           <  1:1936678/150‑1 (MQ=255)
     gaTCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAgg                                                                                                                                           <  2:1613019/150‑1 (MQ=255)
      aTCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGAACACTGGAggg                                                                                                                                          <  2:477055/150‑1 (MQ=255)
      aTCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGAACACTGGAggg                                                                                                                                          <  1:1701829/150‑1 (MQ=255)
       tCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAgggg                                                                                                                                         <  1:1419834/150‑1 (MQ=255)
       tCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGAACACTGGAgggg                                                                                                                                         <  1:667441/150‑1 (MQ=255)
       tCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGAACACTGGAgggg                                                                                                                                         <  1:1788623/150‑1 (MQ=255)
       tCAGACGTTGACTTTTAAAGTTGATTATATAGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGCAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGAACACTGGAgggg                                                                                                                                         <  2:1767992/150‑1 (MQ=255)
        cAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTTACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGa                                                                                                                                        >  2:659806/1‑150 (MQ=255)
          gACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTATTGTGAATGCGGTAACAAATTTCCATATTAACTATTAATAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGata                                                                                                                                      <  2:245149/150‑1 (MQ=255)
              ttGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCa                                                                                                                                  <  2:1819060/150‑1 (MQ=255)
               tGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCat                                                                                                                                 >  1:1888795/1‑150 (MQ=255)
               tGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTACAGTGTTATGGAGGGGATATTCat                                                                                                                                 >  1:67808/1‑150 (MQ=255)
                  cTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGTGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACACCTCCAGTGTTCTGGAGGGGATATTCATAtt                                                                                                                              >  1:438816/1‑150 (MQ=255)
                   ttttAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCTGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATAttt                                                                                                                             <  2:765401/150‑1 (MQ=255)
                    tttAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATAtttt                                                                                                                            <  2:705586/150‑1 (MQ=255)
                      tAAAGTTGATTATATCGCTACTGGGAAAGCGACTTCAGGTAATGTTAATGCTGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCACTCCAGTTTTCTGGAGTGGATATTCATAtttttt                                                                                                                          <  2:1467899/150‑1 (MQ=255)
                        aaGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTaa                                                                                                                        <  2:1164424/150‑1 (MQ=255)
                         aGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATCGGAATGCGGTAACAAAATTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAag                                                                                                                       <  2:395490/150‑1 (MQ=255)
                         aGTTGATTATATCGCTACGGGGAAAGCGACATCAGGTAATGTGAATGCGGTAACAAAATTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAag                                                                                                                       <  1:365799/150‑1 (MQ=255)
                          gTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAaga                                                                                                                      >  2:435841/1‑150 (MQ=255)
                           ttGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAagag                                                                                                                     <  1:703952/150‑1 (MQ=255)
                                  tatCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTAt                                                                                                              >  2:939320/1‑150 (MQ=255)
                                  tatCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGCGGATATTCATATTTTTTAAGAGTGACTAt                                                                                                              >  2:1248556/1‑150 (MQ=255)
                                      gCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTCACTATTTAt                                                                                                          >  2:1499391/1‑150 (MQ=255)
                                              gAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTTTTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACtt                                                                                                  >  2:929403/1‑150 (MQ=255)
                                               aaaGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTg                                                                                                 >  2:656722/1‑150 (MQ=255)
                                                aaGCGACTTCAGGTAATGTGAAAGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGATTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGc                                                                                                <  2:922970/150‑1 (MQ=255)
                                                 aGCGACTTCAGGTAATGTTAATTCTGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCTAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCa                                                                                               <  1:167136/150‑1 (MQ=255)
                                                 aGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCa                                                                                               >  2:1306804/1‑150 (MQ=255)
                                                 aGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCAAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGAATATTTATGAAAACTTgaa                                                                                               >  1:1426927/1‑148 (MQ=255)
                                                     aCTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTTTTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAAc                                                                                           <  2:1952766/150‑1 (MQ=255)
                                                      cTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACa                                                                                          <  1:1789488/150‑1 (MQ=255)
                                                        tCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACaaa                                                                                        >  1:904783/1‑150 (MQ=255)
                                                         cAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTAATATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAg                                                                                       <  2:13949/150‑1 (MQ=255)
                                                          aGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTGGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAgg                                                                                      <  1:941420/150‑1 (MQ=255)
                                                          aGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAgg                                                                                      <  1:348381/150‑1 (MQ=255)
                                                          aGGTAATGTGAATGAGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTATGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAgg                                                                                      <  1:106316/150‑1 (MQ=255)
                                                           ggTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAggg                                                                                     >  1:69262/1‑150 (MQ=255)
                                                           ggTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAggg                                                                                     >  1:790089/1‑150 (MQ=255)
                                                           ggTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAggg                                                                                     >  2:1551068/1‑150 (MQ=255)
                                                           ggTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAggg                                                                                     >  1:98681/1‑150 (MQ=255)
                                                                   gAATTCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGa                                                                             <  2:459017/150‑1 (MQ=255)
                                                                     attctgTAACAAATTTCAATAATAACAATTATAAATAGAACTCATTAATTGTTTTATAAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGAcc                                                                           <  1:1863306/145‑1 (MQ=255)
                                                                      tGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCg                                                                          >  1:1994217/1‑150 (MQ=255)
                                                                        cGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTa                                                                        >  1:1473444/1‑150 (MQ=255)
                                                                              cAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCAt                                                                                              >  1:1228777/1‑122 (MQ=255)
                                                                              cAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCAt                                                                                              <  2:1228777/122‑1 (MQ=255)
                                                                              cAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTa                                                                  >  1:480375/1‑150 (MQ=255)
                                                                              cAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTa                                                                  >  2:1029801/1‑150 (MQ=255)
                                                                                aaTTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGGTTAAc                                                                <  1:1383257/150‑1 (MQ=255)
                                                                                  tttCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACgg                                                              <  2:1000565/150‑1 (MQ=255)
                                                                                    tacATATTAACTATTATTAATATAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCa                                                            <  1:1752472/148‑1 (MQ=255)
                                                                                        tatTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTATGCAGGGGATATTCATTTTTTTTAAGATTTACTATTTATGAAAACTTGAATAACAAAGGGAATTGTTACCGTAAGTTTAAAGGCAatat                                                        >  1:1078916/1‑150 (MQ=255)
                                                                                         atTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACACCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGATTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATAtt                                                       >  1:375384/1‑150 (MQ=255)
                                                                                          ttAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTa                                                      >  1:874596/1‑150 (MQ=255)
                                                                                           tAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTACGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTAc                                                     <  2:194698/150‑1 (MQ=255)
                                                                                            aaCTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTAca                                                    >  1:195726/1‑149 (MQ=255)
                                                                                             aCTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTc                                                   <  1:1742410/150‑1 (MQ=255)
                                                                                              cTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCa                                                  <  2:1408934/150‑1 (MQ=255)
                                                                                               tattatTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTTACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAAAGGCAATATTAATCAg                                                 >  1:1089997/1‑150 (MQ=255)
                                                                                                  tattaATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAgctg                                              >  2:22092/1‑150 (MQ=255)
                                                                                                   attaATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAgctgc                                             >  1:74253/1‑150 (MQ=255)
                                                                                                          gAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCg                                      >  2:483416/1‑150 (MQ=255)
                                                                                                          gAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCg                                      >  1:1982502/1‑150 (MQ=255)
                                                                                                             cTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCAt                                   >  2:1316988/1‑150 (MQ=255)
                                                                                                               cATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAAATTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGATCAACCGCATgg                                 >  2:341461/1‑150 (MQ=255)
                                                                                                                  tAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGggcg                              <  2:1994217/150‑1 (MQ=255)
                                                                                                                  tAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGggcg                              >  1:398519/1‑150 (MQ=255)
                                                                                                                    aTTGTTTTATTAATTAGTACCCGTCGAGTGTGCTGGAGGGGATATTCATATTTTATAAGAGTGCCTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTACGTTTCACGGCAATATTACTCAGCTGCTCATCCGCATGggcggc                            <  2:302746/150‑1 (MQ=255)
                                                                                                                     ttGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCa                           <  1:795177/150‑1 (MQ=255)
                                                                                                                       gTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAgg                         >  1:658773/1‑150 (MQ=255)
                                                                                                                       gTTTAATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACATCCATAACAAACGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAgg                         <  2:106792/150‑1 (MQ=255)
                                                                                                                        ttttATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGt                        >  1:31447/1‑150 (MQ=255)
                                                                                                                         tttATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACCAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTa                       <  1:300308/150‑1 (MQ=255)
                                                                                                                          ttattaATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTaa                      >  2:1094893/1‑150 (MQ=255)
                                                                                                                          ttattaATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTaa                      >  1:481083/1‑150 (MQ=255)
                                                                                                                            attaatttGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAg                    >  1:569139/1‑150 (MQ=255)
                                                                                                                              taattaGTACCCCTCCAGTGTTCTGGAGGGGATATTCATTTTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGc                  >  2:84456/1‑150 (MQ=255)
                                                                                                                                   aGTACCCCTCCAGGGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGAt             <  1:1491178/150‑1 (MQ=255)
                                                                                                                                    ttACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGAtt            <  1:251358/149‑1 (MQ=255)
                                                                                                                                    tatccccTCCAGAACACTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGAtt            >  2:1559071/4‑150 (MQ=255)
                                                                                                                                    gTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGATTTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGAtt            >  1:1257731/1‑150 (MQ=255)
                                                                                                                                    gTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGAtt            >  2:340877/1‑150 (MQ=255)
                                                                                                                                      aCCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTgg          <  1:1729108/150‑1 (MQ=255)
                                                                                                                                      aCCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGAGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTgg          <  2:978307/150‑1 (MQ=255)
                                                                                                                                       ccccTCCAGAACACTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGa         >  2:36828/1‑150 (MQ=255)
                                                                                                                                       ccccTCCAGAACACTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGa         >  2:824486/1‑150 (MQ=255)
                                                                                                                                        cccTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCAGAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGAc        >  1:441280/1‑150 (MQ=255)
                                                                                                                                         ccTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGACt       >  2:23313/1‑150 (MQ=255)
                                                                                                                                         ccTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGAACGTAAGTTTAACGGAAATATTACTCAGCTGCTCATCCGCATGGGCGGAAGTTAAAGGCGGGATTCGACt       >  1:1245378/1‑150 (MQ=255)
                                                                                                                                          cTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGACtt      >  1:436120/1‑150 (MQ=255)
                                                                                                                                            ccAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGACTTgc    >  2:1797837/1‑150 (MQ=255)
                                                                                                                                              aGTGTTCTGGAGGGGATATTCATATTTATTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTTTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGACTTgcag  <  2:91595/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
ATGGAGATCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGACTTGCAG  >  NC_000913/998304‑998595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: