Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 998,451 T→A 5.5% intergenic (+44/‑39) elfA → / → elfD laminin‑binding fimbrin subunit/putative periplasmic pilin chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913998,4510TA 5.5% 449.4 / 9.5 154intergenic (+44/‑39)elfA/elfDlaminin‑binding fimbrin subunit/putative periplasmic pilin chaperone
Reads supporting (aligned to +/- strand):  ref base T (67/77);  new base A (3/6);  total (70/84)
Fisher's exact test for biased strand distribution p-value = 5.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 156 total aligned reads displayed.

ATGGAGATCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGACTTGCAGCAACA  >  NC_000913/998304‑998600
                                                                                                                                                   |                                                                                                                                                     
aTGGAGATCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCt                                                                                                                                                     <  1:212312/150‑1 (MQ=255)
  ggAGATCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACACCTCCAGTGTTCTgg                                                                                                                                                   >  2:1093054/1‑150 (MQ=255)
  ggAGATCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACCATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTgg                                                                                                                                                   >  2:1865903/1‑150 (MQ=255)
     gaTCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCTGTAACAAATTTCCATATTAACTTTTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAgg                                                                                                                                                <  1:1093925/150‑1 (MQ=255)
      aTCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGAACACTGGAggg                                                                                                                                               <  2:477055/150‑1 (MQ=255)
      aTCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGAACACTGGAggg                                                                                                                                               <  1:1701829/150‑1 (MQ=255)
      aTCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGGGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGAACACTGGAggg                                                                                                                                               <  1:449028/150‑1 (MQ=255)
       tCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAgggg                                                                                                                                              <  1:1419834/150‑1 (MQ=255)
       tCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGAACACTGGAgggg                                                                                                                                              <  1:667441/150‑1 (MQ=255)
       tCAGACGTTGACTTTTAAAGTTGATTATATAGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGCAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGAACACTGGAgggg                                                                                                                                              <  2:1767992/150‑1 (MQ=255)
        cAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTTACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGa                                                                                                                                             >  2:659806/1‑150 (MQ=255)
            cGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATAtt                                                                                                                                         <  2:1464390/150‑1 (MQ=255)
              ttGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCa                                                                                                                                       <  2:1819060/150‑1 (MQ=255)
              ttGACTTTTAAAGTTGATTATATCGATACGGTGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCa                                                                                                                                       <  2:326416/150‑1 (MQ=255)
               tGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCat                                                                                                                                      >  1:1888795/1‑150 (MQ=255)
               tGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTACAGTGTTATGGAGGGGATATTCat                                                                                                                                      >  1:67808/1‑150 (MQ=255)
                  cTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGTGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACACCTCCAGTGTTCTGGAGGGGATATTCATAtt                                                                                                                                   >  1:438816/1‑150 (MQ=255)
                  cTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATAtt                                                                                                                                   >  1:1810763/1‑150 (MQ=255)
                   ttttAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCTGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATAttt                                                                                                                                  <  2:765401/150‑1 (MQ=255)
                      tAAAGTTGATTATATCGCTACTGGGAAAGCGACTTCAGGTAATGTTAATGCTGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCACTCCAGTTTTCTGGAGTGGATATTCATAtttttt                                                                                                                               <  2:1467899/150‑1 (MQ=255)
                      tAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATAtttttt                                                                                                                               >  2:1015808/1‑150 (MQ=255)
                      tAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATAAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATAtttttt                                                                                                                               <  1:3064/150‑1 (MQ=255)
                       aaaGTTGATTATATCGCGACGGGGAAAGCGACTTCAGGTAATGTGAATGCTGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCAAGTGTTCTGGAGGGGATATTCATATTTTTTa                                                                                                                              >  1:536763/1‑150 (MQ=255)
                        aaGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTaa                                                                                                                             <  2:1164424/150‑1 (MQ=255)
                         aGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATCGGAATGCGGTAACAAAATTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAag                                                                                                                            <  2:395490/150‑1 (MQ=255)
                          gTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAaga                                                                                                                           >  2:435841/1‑150 (MQ=255)
                           ttGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAagag                                                                                                                          <  1:703952/150‑1 (MQ=255)
                                tataTCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACt                                                                                                                     >  1:95639/1‑150 (MQ=255)
                                  tatCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTAt                                                                                                                   >  2:939320/1‑150 (MQ=255)
                                     cGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCTGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTa                                                                                                                <  1:1756603/150‑1 (MQ=255)
                                     cGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTa                                                                                                                <  1:914123/150‑1 (MQ=255)
                                         aCGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGaa                                                                                                            <  2:1140044/150‑1 (MQ=255)
                                           ggggAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTAATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGaaaa                                                                                                          <  2:2018735/150‑1 (MQ=255)
                                              gAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTTTTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACtt                                                                                                       >  2:929403/1‑150 (MQ=255)
                                                aaGCGACTTCATGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGc                                                                                                     <  2:549246/150‑1 (MQ=255)
                                                aaGCGACTTCAGGTAATGTGAAAGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGATTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGc                                                                                                     <  2:922970/150‑1 (MQ=255)
                                                 aGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCa                                                                                                    >  2:1306804/1‑150 (MQ=255)
                                                     aCTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTTTTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAAc                                                                                                <  2:1952766/150‑1 (MQ=255)
                                                     aCTACACGTAATGTGAATGCAGAAACAAAAATCCAAAATAACTATAATTAATATAACTCAATAATTTTATTATTAATAAGTACCCCACCATTGATCACGAGGGGATAAACAAATTATTTAAGAGTGACTATTTATGAAAACATGCATAAc                                                                                                <  1:246487/150‑1 (MQ=255)
                                                      cTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACa                                                                                               <  1:1789488/150‑1 (MQ=255)
                                                      cTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACa                                                                                               >  2:1311479/1‑150 (MQ=255)
                                                       ttCAGGTAATGAGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACaa                                                                                              >  2:1797112/1‑150 (MQ=255)
                                                         cAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTAATATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAg                                                                                            <  2:13949/150‑1 (MQ=255)
                                                          aGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAgg                                                                                           <  1:348381/150‑1 (MQ=255)
                                                          aGGTAATGTGAATGAGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTATGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAgg                                                                                           <  1:106316/150‑1 (MQ=255)
                                                           ggTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAggg                                                                                          >  1:790089/1‑150 (MQ=255)
                                                           ggTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAggg                                                                                          >  1:98681/1‑150 (MQ=255)
                                                                   taaTGAGGTAAAAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTATTAAGAGTGACTATTTATGAAAACTTGCATAACAAATGGAATTGTGa                                                                                  <  1:698586/149‑1 (MQ=255)
                                                                   gAATTCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGa                                                                                  <  2:459017/150‑1 (MQ=255)
                                                                      tGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCg                                                                               >  1:1994217/1‑150 (MQ=255)
                                                                        cGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTa                                                                             >  1:1473444/1‑150 (MQ=255)
                                                                            aaCTAATTTCCATATTAACTATTTTTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGtt                                                                         <  1:1453464/150‑1 (MQ=255)
                                                                             aCAAATTTCCATATTAACTATTATTAATAGTACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGttt                                                                        <  2:343064/150‑1 (MQ=255)
                                                                              cAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCAt                                                                                                   <  2:1228777/122‑1 (MQ=255)
                                                                              cAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCAt                                                                                                   >  1:1228777/1‑122 (MQ=255)
                                                                              cAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTa                                                                       >  1:480375/1‑150 (MQ=255)
                                                                              cAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTa                                                                       >  2:1029801/1‑150 (MQ=255)
                                                                                aaTTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGGTTAAc                                                                     <  1:1383257/150‑1 (MQ=255)
                                                                                  tttCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATTAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACgg                                                                   <  1:1499391/150‑1 (MQ=255)
                                                                                  tttCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACgg                                                                   <  2:1229410/150‑1 (MQ=255)
                                                                                   ttCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGc                                                                  <  1:362827/150‑1 (MQ=255)
                                                                                      cATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTTTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGTCCGTAAGTTTAACGGCAat                                                               <  1:843942/150‑1 (MQ=255)
                                                                                      cATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAat                                                               <  1:388608/150‑1 (MQ=255)
                                                                                      cATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAat                                                               <  1:1733266/150‑1 (MQ=255)
                                                                                        tatTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTATGCAGGGGATATTCATTTTTTTTAAGATTTACTATTTATGAAAACTTGAATAACAAAGGGAATTGTTACCGTAAGTTTAAAGGCAatat                                                             >  1:1078916/1‑150 (MQ=255)
                                                                                         atTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACACCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGATTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATAtt                                                            >  1:375384/1‑150 (MQ=255)
                                                                                          ttAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTa                                                           >  1:874596/1‑150 (MQ=255)
                                                                                           tAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTAc                                                          <  1:1909376/150‑1 (MQ=255)
                                                                                            aaCTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTAca                                                         >  1:195726/1‑149 (MQ=255)
                                                                                            aaCTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACt                                                         >  2:1175733/1‑150 (MQ=255)
                                                                                             aCTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTc                                                        <  1:1742410/150‑1 (MQ=255)
                                                                                               tattatTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTTACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAAAGGCAATATTAATCAg                                                      >  1:1089997/1‑150 (MQ=255)
                                                                                                attattAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAgc                                                     >  2:1341033/1‑150 (MQ=255)
                                                                                                      aaTAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTTCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCa                                               >  1:1671773/1‑150 (MQ=255)
                                                                                                          gAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCg                                           <  2:674447/150‑1 (MQ=255)
                                                                                                          gAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCg                                           >  2:483416/1‑150 (MQ=255)
                                                                                                               cATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAAATTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGATCAACCGCATgg                                      >  2:341461/1‑150 (MQ=255)
                                                                                                                  tAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGggcg                                   >  1:398519/1‑150 (MQ=255)
                                                                                                                  tAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGggcg                                   <  2:1994217/150‑1 (MQ=255)
                                                                                                                    aTTGTTTTATTAATTAGTACCCGTCGAGTGTGCTGGAGGGGATATTCATATTTTATAAGAGTGCCTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTACGTTTCACGGCAATATTACTCAGCTGCTCATCCGCATGggcggc                                 <  2:302746/150‑1 (MQ=255)
                                                                                                                     ttGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCa                                <  1:437185/150‑1 (MQ=255)
                                                                                                                       gTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAgg                              >  1:658773/1‑150 (MQ=255)
                                                                                                                         tttATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACCAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTa                            <  1:300308/150‑1 (MQ=255)
                                                                                                                         tttATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTa                            <  2:1533524/150‑1 (MQ=255)
                                                                                                                            attaatttGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAg                         >  1:569139/1‑150 (MQ=255)
                                                                                                                              taattaGTACCCCTCCAGTGTTCTGGAGGGGATATTCATTTTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGc                       >  2:84456/1‑150 (MQ=255)
                                                                                                                               aattaGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCg                      >  1:1977314/1‑150 (MQ=255)
                                                                                                                                 ataGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCggg                    <  1:757935/149‑1 (MQ=255)
                                                                                                                                   aGTACCCCTCCAGGGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGAt                  <  1:1491178/150‑1 (MQ=255)
                                                                                                                                    tatccccTCCAGAACACTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGAtt                 >  2:1559071/4‑150 (MQ=255)
                                                                                                                                    gTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGATTTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGAtt                 >  1:1257731/1‑150 (MQ=255)
                                                                                                                                    gTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGAtt                 >  2:1578996/1‑150 (MQ=255)
                                                                                                                                      aCCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGAGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTgg               <  2:978307/150‑1 (MQ=255)
                                                                                                                                       ccccTCCAGTGTTCGGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGa              <  2:108386/150‑1 (MQ=255)
                                                                                                                                       ccccTCCAGAACACTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGa              >  2:36828/1‑150 (MQ=255)
                                                                                                                                       ccccTCCAGAACACTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGa              >  2:824486/1‑150 (MQ=255)
                                                                                                                                         ccTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGACt            >  2:23313/1‑150 (MQ=255)
                                                                                                                                         ccTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGAACGTAAGTTTAACGGAAATATTACTCAGCTGCTCATCCGCATGGGCGGAAGTTAAAGGCGGGATTCGACt            >  1:1245378/1‑150 (MQ=255)
                                                                                                                                            ccAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGACTTgc         <  2:243188/150‑1 (MQ=255)
                                                                                                                                              aGTGTTCTGGAGGGGATATTCATATTTATTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTTTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGACTTgcag       <  2:91595/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
ATGGAGATCAGACGTTGACTTTTAAAGTTGATTATATCGCTACGGGGAAAGCGACTTCAGGTAATGTGAATGCGGTAACAAATTTCCATATTAACTATTATTAATAGAACTCATTAATTGTTTTATTAATTAGTACCCCTCCAGTGTTCTGGAGGGGATATTCATATTTTTTAAGAGTGACTATTTATGAAAACTTGCATAACAAAGGGAATTGTGACCGTAAGTTTAACGGCAATATTACTCAGCTGCTCATCCGCATGGGCGGCAGGTAAAGGCGGGATTGGACTTGCAGCAACA  >  NC_000913/998304‑998600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: