Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,129,394 G→C 5.2% intergenic (+20/+20) murJ → / ← flgN putative lipid II flippase/export chaperone for FlgK and FlgL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,129,3940GC 5.2% 362.6 / 7.4 110intergenic (+20/+20)murJ/flgNputative lipid II flippase/export chaperone for FlgK and FlgL
Reads supporting (aligned to +/- strand):  ref base G (66/38);  new base C (4/2);  total (70/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

GTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATCTGTCCTTCCAG  >  NC_000913/1129245‑1129542
                                                                                                                                                     |                                                                                                                                                    
gTCATTGGGTACCATGCCCTGGCGTTTACTGCGTGTAATGGCGGGCGGGCGGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGGGTAACAATGCATTCCGGCCtgcag                                                                                                                                                      <  2:1763653/150‑1 (MQ=255)
 tCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtgcagt                                                                                                                                                     >  1:1183614/1‑150 (MQ=255)
  ctttttttACCTTGCGCTGGAGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCTCTGCACTGGCGTTACTGGGCTTCAAAGTTAAAGAATTTGGCCGCCGGACGGTGTAACAATGCATTCGTGCAtgcagtg                                                                                                                                                    <  2:1370554/143‑1 (MQ=255)
       ggTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGc                                                                                                                                               >  2:1910043/1‑150 (MQ=255)
          aCCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCTGCCTGCACTGCAGGCCgg                                                                                                                                            <  1:268039/150‑1 (MQ=255)
            cATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCgggg                                                                                                                                          <  1:1667993/150‑3 (MQ=255)
            cATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCAGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGgag                                                                                                                                          >  1:1475665/1‑150 (MQ=255)
                  cTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATc                                                                                                                                    <  2:1659029/150‑1 (MQ=255)
                  cTGGCGGTTACTGCGTTTAATGGCGGGCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATc                                                                                                                                    <  1:1444920/150‑1 (MQ=255)
                      cGTTTACTGCGTTTAATGGCGGTCGTGCGGGCGGGGCTTGCCGCGTACTGCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAATAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCa                                                                                                                                <  2:17186/150‑1 (MQ=255)
                            cTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCAATCCGGCCTGCAGTGCAGGACGGAGATAATCTTCAGATCGa                                                                                                                          >  2:555438/1‑150 (MQ=255)
                               cGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAAt                                                                                                                       <  1:1271359/150‑1 (MQ=255)
                               cGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAAt                                                                                                                       <  1:1645275/150‑1 (MQ=255)
                               cGTTTAATGGCGGTCGTGCTGGCGGGGATTGCAGCGTACTTCGCTGAACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGCTGTAACAATGCATTCCGGACTGCAGTGCAGGCCGGAGATAATCTTCTGCTCGAAAt                                                                                                                       >  1:448057/1‑150 (MQ=255)
                                  ttAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGACTTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCtt                                                                                                                    <  1:201309/150‑1 (MQ=255)
                                  ttAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGGCGGACGGTGTAACAATGCATTCCGGCCTGCTGTGCCGGCCGGAGATAATCTTCTGATCGAAATCtt                                                                                                                    >  1:719602/1‑150 (MQ=255)
                                   tAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCttt                                                                                                                   >  1:1466236/1‑150 (MQ=255)
                                    aaTGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTCACGATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCtttt                                                                                                                  >  1:303681/1‑150 (MQ=255)
                                      tGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTa                                                                                                                <  2:407875/150‑1 (MQ=255)
                                      tGGCGCTCGTGCTGGCGGTGTTTTCCTCGTACTTTGCTGCACTGGCGGTACAGGGCTTCAAAGTTAAAGAATTTGCCCGACGGACGGTGTAAGAATGGATTCCGGCCTGCTGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTa                                                                                                                <  1:151414/150‑1 (MQ=255)
                                           gTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTAccgcc                                                                                                           <  1:589075/150‑1 (MQ=255)
                                               tGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGa                                                                                                       >  1:1559572/1‑150 (MQ=255)
                                                 cTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTAGTGGGGTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTTACACTGCATTCCGGCCTGCAGTGCGGGCCGGGGAGAATCTTCAGATCGAAATCTTTTTTCAGCCGCGATg                                                                                                     >  1:279029/1‑150 (MQ=255)
                                                     cgcggAATGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAATGAATTTGCCCGCCGGACGTTGTTACAAAGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCATCAGATCGAAATCTTTATACCGACGCGATGGctt                                                                                                 >  1:1611859/4‑150 (MQ=255)
                                                     cGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATTATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGgtt                                                                                                 >  1:1829555/1‑150 (MQ=255)
                                                        ggATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGgttgtt                                                                                              >  2:1290710/1‑150 (MQ=255)
                                                          tttGCCGCGTAGTTCGCTGGAGTGGCGGTCCTGGGCTTCAAAGTTAACGAATTTGGCCGACGGACGGTGTAACTATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGgttgttta                                                                                            <  1:223192/149‑3 (MQ=255)
                                                            tGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAgg                                                                                          <  1:1174540/150‑1 (MQ=255)
                                                             gCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGt                                                                                         >  2:219075/1‑150 (MQ=255)
                                                             gCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGt                                                                                         >  2:866057/1‑150 (MQ=255)
                                                              ccGCGTTCTTCGCGGCACTGGCGGTACTGGGCTTCAAAGCTAAAGACTTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATATTCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTc                                                                                        <  1:1039434/150‑1 (MQ=255)
                                                              ccGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTc                                                                                        <  2:1690581/150‑1 (MQ=255)
                                                               cgcgTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCt                                                                                       <  1:2007510/150‑1 (MQ=255)
                                                               cgcgTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCt                                                                                       >  2:278977/1‑150 (MQ=255)
                                                                gcgTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTg                                                                                      <  2:1680608/150‑1 (MQ=255)
                                                                gcgTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGGTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTg                                                                                      <  1:1677027/150‑1 (MQ=255)
                                                                  gTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGAc                                                                                    >  2:47875/1‑150 (MQ=255)
                                                                   tACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGAcc                                                                                   <  2:597189/150‑1 (MQ=255)
                                                                   tACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGGTGAGGTCTGAcc                                                                                   >  1:1009721/1‑150 (MQ=255)
                                                                    aCTTCGCTGCACTGGCGGTTCTGGTCTTCAAAGTTTAAGAATTTGCCCGCCGGACGTTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATTATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCg                                                                                  <  1:1801914/150‑1 (MQ=255)
                                                                     cTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGCTCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGt                                                                                 >  1:969460/1‑150 (MQ=255)
                                                                     cTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGt                                                                                 <  2:1876149/150‑1 (MQ=255)
                                                                       tCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTc                                                                               <  2:702698/150‑1 (MQ=255)
                                                                        cGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCg                                                                              <  1:222564/150‑1 (MQ=255)
                                                                         gCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTTCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGACGGAGATAATCTTCAGATAGAAATCTTTTTACCGCCGCGAAGGGTTGTTGAGTTCTGCCCGTTCGc                                                                             >  1:341797/1‑150 (MQ=255)
                                                                          cTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCTGGCCGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCAGGAGATAATCTTCTGATCGAAATCTTTTTACCGCAGCGATGTGGTGTTGAGGTCTGACCGTTCGcc                                                                            >  1:1269015/1‑150 (MQ=255)
                                                                           tGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGccc                                                                           >  2:954814/1‑150 (MQ=255)
                                                                           tGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGCAGATAATCTTCATATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGccc                                                                           >  2:1613338/1‑150 (MQ=255)
                                                                            gCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGcccc                                                                          >  2:1858514/1‑150 (MQ=255)
                                                                              aCTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGcacaat                                                                        >  2:282397/1‑145 (MQ=255)
                                                                               cTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCata                                                                       >  2:434926/1‑150 (MQ=255)
                                                                                   cGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGc                                                                   >  2:1675468/1‑150 (MQ=255)
                                                                                       aCTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCg                                                               <  2:1390469/150‑1 (MQ=255)
                                                                                            gCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCTGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTcc                                                          <  1:1235837/150‑1 (MQ=255)
                                                                                            gCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTcc                                                          >  1:1743043/1‑150 (MQ=255)
                                                                                            gCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCGTCAGATCGAAATCTTTTTAACGCCGCGATGGGTTGTTGAGGTCTGCCCGTTCGCCCCATATAGCGTCGGttgc                                                          >  1:1905089/1‑148 (MQ=255)
                                                                                             cTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCt                                                         >  1:745464/1‑150 (MQ=255)
                                                                                               tCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGa                                                       >  1:631280/1‑150 (MQ=255)
                                                                                                cAAAGTTTAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATATCGTCGGTTACTGAt                                                      >  2:1651661/1‑150 (MQ=255)
                                                                                                cAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGAt                                                      >  2:1036533/1‑150 (MQ=255)
                                                                                                   aGTTAAAGAATTTGCCCGCCGGACGGTGTAAAAATGCATTCCGGCCTGCAGTGCATGCCGGAGATAATCTTCAGATAGAAATATTTTTACCGCCGCGATGGGTTGTTGAGGTATGACCGTTCGCCCCATATAGATTCGGTTCCTGATGCg                                                   >  1:1630966/1‑150 (MQ=255)
                                                                                                     ttAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCCNNAGTGCANGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGt                                                 >  1:1625225/1‑150 (MQ=255)
                                                                                                     ttAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCAGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGt                                                 >  1:1633620/1‑150 (MQ=255)
                                                                                                      tAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGAAGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGtt                                                >  2:1572390/1‑150 (MQ=255)
                                                                                                       aaaGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGttt                                               >  1:678842/1‑150 (MQ=255)
                                                                                                       aaaGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGttt                                               >  2:631772/1‑150 (MQ=255)
                                                                                                        aaGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCATCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTAGAGGTCTGACCGTTCGCCCCATATAGAGTCGGTTACTGATGCGGTTTc                                              >  2:1599217/1‑150 (MQ=255)
                                                                                                         aGAATTTGCCCGCCTGACGGTGTAACAATGCTTTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCa                                             <  1:20911/150‑1 (MQ=255)
                                                                                                         aGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGATGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCa                                             <  2:1747491/150‑1 (MQ=255)
                                                                                                         aGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCa                                             <  2:636775/150‑1 (MQ=255)
                                                                                                           aaTTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGGGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAAc                                           <  1:233557/150‑1 (MQ=255)
                                                                                                            aTTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACa                                          <  1:1105720/150‑1 (MQ=255)
                                                                                                            aTTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACa                                          <  1:1863201/150‑1 (MQ=255)
                                                                                                               tGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATTATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCAGATAGCGTCGGTTCCGGATGCGGTTTCAACAatt                                       >  2:764062/1‑150 (MQ=255)
                                                                                                                gCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTc                                      >  2:1902312/1‑150 (MQ=255)
                                                                                                                gCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGATCCTGATGCGGTTTCAACATTTc                                      >  1:959056/1‑150 (MQ=255)
                                                                                                                     ccgGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGGTGTTGAGGGCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAgcg                                 <  2:574067/150‑1 (MQ=255)
                                                                                                                       ggACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCAGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATTGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGcc                               <  2:1475665/150‑1 (MQ=255)
                                                                                                                           ggTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTGCAGATCGAAATCTATTTACAGCCGCGATGGGTTGTTGAGGTCTGACCGTTAGCCCCATATAGCGTCGGGTGCTGATGCGGTTTCCACATTTCCCGCGCCTgat                           >  1:301304/1‑148 (MQ=255)
                                                                                                                              gtAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATTTAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGAt                        >  1:1898267/1‑150 (MQ=255)
                                                                                                                              gtAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGAGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCATCGCCTGTTGAt                        >  2:1897402/1‑150 (MQ=255)
                                                                                                                               tAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGAtt                       >  2:479112/1‑150 (MQ=255)
                                                                                                                                  cAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGGTGCGGTTTCAACATTTCCAGCGCCTGTTGATTgcg                    >  1:1026427/1‑150 (MQ=255)
                                                                                                                                  cAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTgcg                    >  1:1196738/1‑150 (MQ=255)
                                                                                                                                   aaTGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTgcgc                   >  1:1611342/1‑150 (MQ=255)
                                                                                                                                   aaTGCATTCAGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGTAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTgcgc                   <  2:1525574/150‑1 (MQ=255)
                                                                                                                                    aTGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCt                  <  2:341479/150‑1 (MQ=255)
                                                                                                                                        aTTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAAt              >  1:1019369/1‑150 (MQ=255)
                                                                                                                                        aTTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAAt              >  2:332392/1‑150 (MQ=255)
                                                                                                                                         ttCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATc             <  1:1167547/150‑1 (MQ=255)
                                                                                                                                         ctCCGGCCTGCACTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCGGTTGATTGCGCTCAAtg             >  1:1646793/2‑149 (MQ=255)
                                                                                                                                           ccGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTTCGCTCAATCTg           >  2:934542/1‑150 (MQ=255)
                                                                                                                                           ccGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATCTg           >  2:583827/1‑150 (MQ=255)
                                                                                                                                           ccGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATCTg           >  2:1132961/1‑150 (MQ=255)
                                                                                                                                           ccGGCCTGCACTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATCTg           >  1:694338/1‑150 (MQ=255)
                                                                                                                                           ccGGCCTGCACTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTAGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGGTCAAACTg           >  2:400596/1‑150 (MQ=255)
                                                                                                                                               ccTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATCTGTCCt       >  2:1705636/1‑150 (MQ=255)
                                                                                                                                                 tgcagtgcagGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCACTCTGTCCTTc     >  1:14832/1‑150 (MQ=255)
                                                                                                                                                   cagtgcagGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGATCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATCTGTCCTTCCa   <  2:1522038/150‑1 (MQ=255)
                                                                                                                                                    agtgcagGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATCTGTCCTTCCAg  <  1:1897402/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATCTGTCCTTCCAG  >  NC_000913/1129245‑1129542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: