Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,146,741 A→T 5.4% noncoding (153/169 nt) psrD → novel Novel sRNA, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,146,7410AT 5.4% 349.8 / 5.1 117noncoding (153/169 nt)psrDnovel Novel sRNA, function unknown
Reads supporting (aligned to +/- strand):  ref base A (62/48);  new base T (4/3);  total (66/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

CATATTTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATACCCCTGATCAGGTTGAGC  >  NC_000913/1146593‑1146890
                                                                                                                                                    |                                                                                                                                                     
cATATTTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAg                                                                                                                                                      >  1:676596/1‑150 (MQ=255)
 atatTTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAgg                                                                                                                                                     >  1:86314/1‑150 (MQ=255)
 atatTTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAgg                                                                                                                                                     >  1:1170705/1‑150 (MQ=255)
      tttttCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGcc                                                                                                                                                <  1:1946602/150‑1 (MQ=255)
             aTCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCt                                                                                                                                         >  2:995376/1‑150 (MQ=255)
             aTCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGGTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCt                                                                                                                                         <  2:1384217/150‑1 (MQ=255)
              tCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCtt                                                                                                                                        >  1:1704636/1‑150 (MQ=255)
              tCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGAAGTTTGTTATCTGCACTGAACGAAAATAAAGCAGTACCCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCtt                                                                                                                                        >  2:318981/1‑150 (MQ=255)
               cAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCttt                                                                                                                                       <  2:1815626/150‑1 (MQ=255)
               cAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTACAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAGCCTGTGGTTGCCttttgccaa                                                                                                                                       <  1:1307034/150‑6 (MQ=255)
                aGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTg                                                                                                                                      <  2:1968071/150‑1 (MQ=255)
                aGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAAAGAAAATAATGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTg                                                                                                                                      <  2:29338/150‑1 (MQ=255)
                 gATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGa                                                                                                                                     <  1:689086/150‑1 (MQ=255)
                    ataGCGTTTTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGActc                                                                                                                                  <  2:1589585/150‑1 (MQ=255)
                       gCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGATATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTAt                                                                                                                               >  1:943172/1‑150 (MQ=255)
                             tgatgaTAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGtt                                                                                                                         >  1:1168221/1‑150 (MQ=255)
                             tgatgaTAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTATTTCTTTGACTCTATGACGtt                                                                                                                         >  1:430832/1‑150 (MQ=255)
                              gatgatAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTa                                                                                                                        >  2:917793/1‑150 (MQ=255)
                               atgatAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTAc                                                                                                                       <  2:619669/150‑1 (MQ=255)
                                   tAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAg                                                                                                                   >  1:1766684/1‑150 (MQ=255)
                                    aGCGATTTTAAACTATGCGCTTCGTTTTGGGGGTTTATGTTTGTTATCAGCACTGAACTAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAATGCATCCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGt                                                                                                                  <  1:1920022/150‑1 (MQ=255)
                                       caatttAAACTATGCGGAGCGTTTTGCAGGTGGAGGTTTGCTCTCAGCACTGCACGAACATAAAGCAGTAACCGTCAATGTGTGCGAATTATTGGCAAAAGTAAAACACAGGCTGCCTTTTTCTTTTACTCTATGACTTTACAAAGTTaa                                                                                                               <  2:944915/147‑1 (MQ=255)
                                        aTTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAat                                                                                                              >  1:1676643/1‑150 (MQ=255)
                                        aTTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAat                                                                                                              >  2:1643633/1‑150 (MQ=255)
                                           ttAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATg                                                                                                           <  1:1126694/150‑1 (MQ=255)
                                             aaaCTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATgcg                                                                                                         <  1:1626947/150‑1 (MQ=255)
                                               aCTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATgcgcg                                                                                                       <  1:495339/150‑1 (MQ=255)
                                                cTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATgcgcgc                                                                                                      <  2:385500/150‑1 (MQ=255)
                                                 tATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGcc                                                                                                     <  1:1728576/150‑1 (MQ=255)
                                                   tGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGCACGAAATTAAAGCAGTTACCCGCAATGTGTGCGAATTTTTGGCAAAAGGGAACCACAGGCTGCCTGTTTCTTTGTCTCTATGAGGGTACAAAGTGAGTATGGGGGcccg                                                                                                   >  2:1330273/1‑149 (MQ=255)
                                                     cgcTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGATAATAAAGCAGTACCCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACTGGCTGCGTTTTTCTTTGACTCGATGACGTTAGAAAGTTAATATGCGCGGCGTAt                                                                                                 >  2:1879259/1‑150 (MQ=255)
                                                      gcTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATg                                                                                                >  2:852527/1‑150 (MQ=255)
                                                       cTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGc                                                                                               <  2:839542/150‑1 (MQ=255)
                                                         tCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCaa                                                                                             <  2:2013542/150‑1 (MQ=255)
                                                         tCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTAGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCaa                                                                                             <  2:1404064/150‑1 (MQ=255)
                                                           gTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATTACGTTACAAAGTTAATATGCGCGCCCTATGCaaaa                                                                                           >  1:770166/1‑150 (MQ=255)
                                                           gTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCaaaa                                                                                           <  2:1278856/150‑1 (MQ=255)
                                                             tttGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGTCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAgg                                                                                         >  1:1126638/1‑150 (MQ=255)
                                                             tttGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGACCTATGCAAAAgg                                                                                         >  1:614153/1‑150 (MQ=255)
                                                                   gggtGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAAt                                                                                   <  2:1972168/147‑1 (MQ=255)
                                                                   ggTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAAt                                                                                   <  1:446029/150‑1 (MQ=255)
                                                                   ggTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAAt                                                                                   >  2:416610/1‑150 (MQ=255)
                                                                   ggTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAAt                                                                                   >  2:1846808/1‑150 (MQ=255)
                                                                           tttgttATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTg                                                                           <  2:349127/150‑1 (MQ=255)
                                                                               ttATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGActc                                                                       <  1:1427661/150‑1 (MQ=255)
                                                                                tATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGActct                                                                      >  1:1595418/1‑150 (MQ=255)
                                                                                tATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGActct                                                                      >  1:521544/1‑150 (MQ=255)
                                                                                tATCAGCACTGAACGAAAATAAAGCAGTACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATTCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACtctc                                                                     >  2:956680/1‑150 (MQ=255)
                                                                                     gCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATc                                                                 <  1:1453421/150‑1 (MQ=255)
                                                                                      cACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATcc                                                                >  1:1603669/1‑150 (MQ=255)
                                                                                       aCTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCg                                                               >  1:1126755/1‑150 (MQ=255)
                                                                                       aCTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCg                                                               >  1:1334254/1‑150 (MQ=255)
                                                                                         tGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGACATTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTAAGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGt                                                             >  1:573603/1‑150 (MQ=255)
                                                                                          gAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGtt                                                            <  1:929905/150‑1 (MQ=255)
                                                                                           aaCGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTc                                                           <  2:970112/150‑1 (MQ=255)
                                                                                            acaaaaaTAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCg                                                          <  2:1949846/147‑1 (MQ=255)
                                                                                             cGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGt                                                         >  1:1491510/1‑150 (MQ=255)
                                                                                             cGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGt                                                         >  2:1166823/1‑150 (MQ=255)
                                                                                              taaaaTAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTa                                                        <  1:1910016/149‑1 (MQ=255)
                                                                                              gAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTa                                                        <  1:1704197/150‑1 (MQ=255)
                                                                                               aaaaTAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTAc                                                       >  2:1261173/1‑150 (MQ=255)
                                                                                               aaaaTAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTAc                                                       >  2:1312254/1‑150 (MQ=255)
                                                                                               aaaaTAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTAc                                                       >  1:473109/1‑150 (MQ=255)
                                                                                                aaaTAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACg                                                      <  2:86314/150‑1 (MQ=255)
                                                                                                 aaTAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACgg                                                     >  2:769866/1‑150 (MQ=255)
                                                                                                  aTAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGTCTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGc                                                    <  1:995376/150‑1 (MQ=255)
                                                                                                     aaGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCa                                                 >  1:866759/1‑150 (MQ=255)
                                                                                                      aGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCaa                                                >  1:1949455/1‑150 (MQ=255)
                                                                                                       gCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCaaa                                               >  2:167758/1‑150 (MQ=255)
                                                                                                          gTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAAc                                            >  1:1763440/1‑150 (MQ=255)
                                                                                                             aCCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGCCTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGcc                                         >  2:193696/1‑150 (MQ=255)
                                                                                                              cccGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCt                                        <  1:1137513/150‑1 (MQ=255)
                                                                                                               ccGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCtt                                       >  2:149729/1‑150 (MQ=255)
                                                                                                                cGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTg                                      <  1:1129320/150‑1 (MQ=255)
                                                                                                                    aTGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTa                                  <  2:1766684/150‑1 (MQ=255)
                                                                                                                       tgtgCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCa                               >  2:577749/1‑150 (MQ=255)
                                                                                                                        gtgCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCATATGCAAAAGGTAAAATTACCCCTGAATCTCGATCAGGTTCGTACGGCTCAAAAACGCCTTGATTACCAg                              >  1:933182/1‑150 (MQ=255)
                                                                                                                         tgCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAgg                             <  2:445900/150‑1 (MQ=255)
                                                                                                                          gCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAggg                            <  1:917481/150‑1 (MQ=255)
                                                                                                                          gCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAggg                            >  1:1433753/1‑150 (MQ=255)
                                                                                                                          gCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCAGGTTCGTACTGCTCAAAAACGCCTTGATTACAAggg                            >  1:833552/1‑150 (MQ=255)
                                                                                                                           ggAATTATTGGCAAAAGGTAACCACAGGCTGCAGTTTTCTTTGACTCTATGACGTTACAAATTTATTATTCGCGCACTATGCAAAAGGTAATATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGAGTACCAGGGt                           <  2:745820/149‑1 (MQ=255)
                                                                                                                            gAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTa                          >  1:1976711/1‑150 (MQ=255)
                                                                                                                               ttattGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCt                       <  1:1745701/150‑1 (MQ=255)
                                                                                                                                tattGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCta                      <  2:424682/150‑1 (MQ=255)
                                                                                                                                 caaagaaaaaGGCAGCCTGTGGTTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCtat                     >  2:2435/7‑150 (MQ=255)
                                                                                                                                 attGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGCCTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAATGGTAAAATTACCCCTGCCTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGGAGCtat                     >  2:1011715/1‑150 (MQ=255)
                                                                                                                                  aaagaaaaaGGCAGCCGGTGGTTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGGAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAACGCCTTGATTACCAGGGTATCTATAc                   >  1:223133/6‑150 (MQ=255)
                                                                                                                                   tGGCAAAAGGCAACCACAGGCTGCCTGTTTCGTTGACTCTATGACGTTACAAAGTTAATAGGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCGCAAAAACGCCTTGATTACCAGGGTATCTATAc                   >  1:409866/1‑150 (MQ=255)
                                                                                                                                    ggCAAAAGGCACCCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGAGCGCCCTATGCAAAAGGTAAAATTAACCCGTACTCTCGATCCGGTTAGTACGGCTCAAAAACGCCGTGATTACCAGGGTATCTGTAcc                  >  2:434269/1‑150 (MQ=255)
                                                                                                                                      cAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATAcccc                <  2:1170705/150‑1 (MQ=255)
                                                                                                                                      cAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAAAATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTAGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATAcccc                >  1:1088827/1‑150 (MQ=255)
                                                                                                                                       aaaaGGCAGCCTGTGGTTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATACCCCt               >  1:1747997/1‑150 (MQ=255)
                                                                                                                                       aaaaGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATACCCCt               >  2:1581292/1‑150 (MQ=255)
                                                                                                                                        taacgCAACCACACGCTGCATTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGGCCTATGCAAAATGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATACCCCTg              <  1:24011/146‑1 (MQ=255)
                                                                                                                                          aGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATACCCCTGAt            <  1:2015293/150‑1 (MQ=255)
                                                                                                                                           ggCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATACCCCTGATc           >  1:1195903/1‑150 (MQ=255)
                                                                                                                                           ggCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATACCCCTGATc           >  2:1144172/1‑150 (MQ=255)
                                                                                                                                             cAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATACCCCTGATCAg         <  1:1521919/150‑1 (MQ=255)
                                                                                                                                                 cacaGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATACCCCTGATCAGGTTg     <  2:1788410/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CATATTTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATACCCCTGATCAGGTTGAGC  >  NC_000913/1146593‑1146890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: