Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,260,904 A→G 5.6% intergenic (‑101/+24) dauA ← / ← prs C4‑dicarboxylic acid transporter/phosphoribosylpyrophosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,260,9040AG 5.6% 366.6 / 8.8 126intergenic (‑101/+24)dauA/prsC4‑dicarboxylic acid transporter/phosphoribosylpyrophosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (74/44);  new base G (4/3);  total (78/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.75e-01

Only 100 of 129 total aligned reads displayed.

CGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCTCAGCGGAAT  >  NC_000913/1260755‑1261053
                                                                                                                                                      |                                                                                                                                                     
cGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCa                                                                                                                                                       >  2:746704/1‑150 (MQ=255)
 gATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAg                                                                                                                                                      >  1:1047736/1‑150 (MQ=255)
 gATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAg                                                                                                                                                      >  1:1417272/1‑150 (MQ=255)
    cAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCgg                                                                                                                                                   >  1:107489/1‑150 (MQ=255)
     agagCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCggg                                                                                                                                                  >  1:393901/1‑150 (MQ=255)
     agagCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCggg                                                                                                                                                  <  2:1887330/150‑1 (MQ=255)
       agCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTc                                                                                                                                                >  2:1744114/1‑150 (MQ=255)
         cgcgGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCtt                                                                                                                                              <  1:1857085/150‑1 (MQ=255)
         cgcgGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCtt                                                                                                                                              >  1:1059320/1‑150 (MQ=255)
           cgGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCTGCGGCGGGTttttt                                                                                                                                            <  1:2017486/150‑2 (MQ=255)
           cgGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTg                                                                                                                                            >  2:1247986/1‑150 (MQ=255)
           cgGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGGGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTg                                                                                                                                            <  1:1400221/150‑1 (MQ=255)
           cgGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAAAAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCAAACAAATGGATAATACAGACAAAAAAACCCGCAGCAGCGGCTCTTTg                                                                                                                                            >  2:201326/1‑150 (MQ=255)
             gAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAg                                                                                                                                          <  1:1522391/150‑1 (MQ=255)
              aaaGGCATCAGATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAATTGGCAGGTGAAGGAGGCATAGGTCATACTAATGGATATTACAGACAAAAAAACCCGCTGCGGCGGGTttttttgt                                                                                                                                         <  1:1604795/150‑5 (MQ=255)
              aaaGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGc                                                                                                                                         <  1:1313324/150‑1 (MQ=255)
                  gCATCACATTTGAGGAAAATATTTTGCTCACAAAAATGTTTCATCCGTGAGCGCATCATCTGTCAATTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCggg                                                                                                                                     <  2:372103/150‑1 (MQ=255)
                   cATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGt                                                                                                                                    >  2:945413/1‑150 (MQ=255)
                   cATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACACGCCGCAGCGGGTCTTTGAGCCGGGt                                                                                                                                    >  2:899299/1‑150 (MQ=255)
                    aTCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGtt                                                                                                                                   <  2:1723527/150‑1 (MQ=255)
                       acaTGTTAGGAAAATATTTTGATCACAATAATCTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGa                                                                                                                                <  1:335514/150‑1 (MQ=255)
                         aTGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGAtt                                                                                                                              <  2:1866141/150‑1 (MQ=255)
                          tgtgAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCCACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTAAAGACAAAAAAACCCGCCGCAGCGGGTCTTTTCTCCGGGTGCGTTTa                                                                                                                             >  2:1941555/1‑150 (MQ=255)
                           gtgAGGAAAATATTTTGTTCACAATAATTTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAg                                                                                                                            <  2:242666/150‑1 (MQ=255)
                           gtgAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAg                                                                                                                            >  2:2012159/1‑150 (MQ=255)
                            tgAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAgt                                                                                                                           >  2:1082019/1‑150 (MQ=255)
                                 aaaaTATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCg                                                                                                                      >  1:608248/1‑150 (MQ=255)
                                 aaaaTATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGACGCAGCGGGTCTTTCAGCAGGGTTCGAATAGTGTTCg                                                                                                                      >  2:495517/1‑150 (MQ=255)
                                 aaaaTATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAAAGGCAGGAGAAGGAGGCATAGGTCAAACAAATCGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCATTGAGCAGGCTTCGATAACTGTTCg                                                                                                                      >  2:502898/1‑150 (MQ=255)
                                    atatTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAAc                                                                                                                   >  2:1484377/1‑150 (MQ=255)
                                        tttGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATgg                                                                                                               <  2:1894154/150‑1 (MQ=255)
                                        tttGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATgg                                                                                                               <  2:1106656/150‑1 (MQ=255)
                                             tCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCagag                                                                                                          >  2:1784439/1‑150 (MQ=255)
                                             tCACAAAAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAAAGCCAGGTGAAGCAGCCATAGGTCATACAAATGGATATTACAGACAAAAAAACCAGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCagac                                                                                                          >  2:1940323/1‑149 (MQ=255)
                                              cacaATATTGTTTCATCCGTGAGCGCATCATCTGTCAACTATATGGCAGGTGAAGGAGGCATAGGTCATACAATTGGATATAACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTGGAACAAGCCagaca                                                                                                         >  2:241636/1‑148 (MQ=255)
                                              cacaATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAgaga                                                                                                         >  2:964580/1‑150 (MQ=255)
                                                caATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATc                                                                                                       >  2:1590416/1‑150 (MQ=255)
                                                caATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAAGGGCAGGTGAAGGAGGCATAGGTCATACAAAAGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATc                                                                                                       <  1:1240914/150‑1 (MQ=255)
                                                   taatGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGAt                                                                                                    <  2:592544/150‑1 (MQ=255)
                                                    aatGTTTGAGCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGAtt                                                                                                   <  1:1106307/150‑1 (MQ=255)
                                                    aatGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGAtt                                                                                                   <  2:1677551/150‑1 (MQ=255)
                                                          tCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACACGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGt                                                                                             <  2:78935/150‑1 (MQ=255)
                                                           cATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGtt                                                                                            >  2:1494074/1‑150 (MQ=255)
                                                            aTCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTg                                                                                           <  1:927108/150‑1 (MQ=255)
                                                             tCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGc                                                                                          <  2:1968361/150‑1 (MQ=255)
                                                               cGTGATCGCATCATCTGTCAACTAAACGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTg                                                                                        <  2:619660/150‑1 (MQ=255)
                                                               cGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTg                                                                                        <  2:39960/150‑1 (MQ=255)
                                                               cGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCCCCCCAGCGGGGCAATGAGCCGGGTTCGATTAGTTTTCCAAAATGGCAGAGAACGATTCATCGTTGCTg                                                                                        >  2:607872/1‑150 (MQ=255)
                                                                  gAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATa                                                                                     >  2:214236/1‑150 (MQ=255)
                                                                   aGCGCATCATCTGTCAACTAACTGCCAGGTGCAGGAGGCATAGGTCATACAAAAGGATATAACAGACAACCACACCCGCCGCAGCGGGTCTTTGTGCCGGGAGCGATAAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATac                                                                                    <  1:287175/150‑1 (MQ=255)
                                                                   aGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATac                                                                                    >  2:17885/1‑150 (MQ=255)
                                                                      gcATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATAATACAGACAAAAAAACCCGCCGAAGCGGGTCTTTGAGCCGGGTTCGATAATTGTTCGATCATGCCAGAGATCGAATCTTCGTTGCTGATacgac                                                                                 >  1:882100/1‑150 (MQ=255)
                                                                       catcatCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATacgacg                                                                                <  2:395836/150‑1 (MQ=255)
                                                                                tCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTc                                                                       <  1:1487508/150‑1 (MQ=255)
                                                                                 cAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGAACGATTCTACGTTGCTGATACGACGAATCGCTACg                                                                      >  2:294937/1‑150 (MQ=255)
                                                                                      aaaTGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGcaac                                                                 >  1:1500141/1‑147 (MQ=255)
                                                                                         tGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCAt                                                              <  1:1711678/150‑1 (MQ=255)
                                                                                          ggCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCCGCATa                                                             >  1:1101217/1‑150 (MQ=255)
                                                                                             aGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCt                                                          >  1:1853077/1‑150 (MQ=255)
                                                                                             aGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCt                                                          >  1:625573/1‑150 (MQ=255)
                                                                                               gTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGa                                                        >  1:1412181/1‑150 (MQ=255)
                                                                                               gTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGa                                                        <  1:645188/150‑1 (MQ=255)
                                                                                                tGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGAc                                                       >  1:1720769/1‑150 (MQ=255)
                                                                                                  aaGGAGGCATAGGTCATACAAATGGATATTACAGGCAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGATCAAGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAg                                                     >  1:868614/1‑150 (MQ=255)
                                                                                                      aggCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTc                                                 <  2:407270/150‑1 (MQ=255)
                                                                                                      aggCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGATTCGGCCAGCATACCTGACAGGGTc                                                 >  1:1224834/1‑150 (MQ=255)
                                                                                                       ggCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCa                                                >  2:50782/1‑150 (MQ=255)
                                                                                                           tAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGt                                            <  2:1047736/150‑1 (MQ=255)
                                                                                                            aGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTa                                           <  1:1494074/150‑1 (MQ=255)
                                                                                                              gTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCTCTTCGGCCAGCATACCTGACAGGGTCAGAGTACg                                         >  1:1827834/1‑150 (MQ=255)
                                                                                                              gTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACg                                         <  2:298230/150‑1 (MQ=255)
                                                                                                               tCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGc                                        >  2:175392/1‑150 (MQ=255)
                                                                                                                  tACATATGGATATTACTGACAAAAAAACCCGCCGCCGCGGGTCTTTGAGCCGGGCTCGATTCGTGTGCGAACATGGCAGAGATCGATTCTGCGATGCTGATACGACGACTCGCTTCGGCCAGCATACCTGACAGCGTCAGAGTACGCAcc                                     <  1:286921/150‑2 (MQ=255)
                                                                                                                  tACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGCGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACg                                     >  1:1480017/1‑150 (MQ=255)
                                                                                                                  tACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACg                                     <  2:558141/150‑1 (MQ=255)
                                                                                                                   aCAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGt                                    >  1:705150/1‑150 (MQ=255)
                                                                                                                    cAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGtt                                   >  2:1401147/1‑150 (MQ=255)
                                                                                                                      aaTGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCg                                 >  1:1929681/1‑150 (MQ=255)
                                                                                                                         ggATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCa                              <  2:494726/150‑1 (MQ=255)
                                                                                                                            ttttACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGCGCCGGGTGCGAGTAGTGTTCGAACATGGCAGCGATCGAGTGTTCGTTGCTGATACGCCGATTAGCTACGGCCAGCATACCTGACAGGGTCAGACTACGCACGTAAGGCAggg                           >  2:320343/3‑148 (MQ=255)
                                                                                                                            tatTACAGACAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGt                            <  1:1674872/150‑1 (MQ=255)
                                                                                                                            tatTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGtg                           >  1:2009731/1‑150 (MQ=255)
                                                                                                                                aCAGACAAAAAAACCCGCCGCAGCGGGGATTTGAGCCGGGTTCGATTAGTGTTCGCACATGGCAGAGATCGATTCTTCGTTGCTGAGACGACGAATCGCATCGGCCATCATACATGACAGGGTAAGAGTACGCAGGTTCGGCCGtgattt                       >  2:1493345/1‑150 (MQ=255)
                                                                                                                                   gACAAAAAAACACGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGtgatttgat                    >  2:637351/1‑150 (MQ=255)
                                                                                                                                    aCAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGtgatttgatt                   <  1:1936956/150‑1 (MQ=255)
                                                                                                                                     gctcaaagaCCCGCTGCGGCGGGTTTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGtgatttgatt                   >  1:372170/5‑150 (MQ=255)
                                                                                                                                     cAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTgatttgattt                  >  2:1988271/1‑150 (MQ=255)
                                                                                                                                     cAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTgatttgattt                  >  2:1490337/1‑150 (MQ=255)
                                                                                                                                     cAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTgatttgattt                  >  1:1114918/1‑150 (MQ=255)
                                                                                                                                      ctcaaagaCCCGCTGCGGCGGGTTTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTgatttgattt                  >  1:1729331/4‑150 (MQ=255)
                                                                                                                                       aaaaaaaCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATAGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGATCGGCAGTGATTTGATTTc                 >  2:1378662/1‑150 (MQ=255)
                                                                                                                                        caaagaCCCGCTGCGGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCa                >  2:719373/2‑150 (MQ=255)
                                                                                                                                          aaaaCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACAGGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATc              <  1:1655200/150‑1 (MQ=255)
                                                                                                                                              cccGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCTc          >  1:562331/1‑150 (MQ=255)
                                                                                                                                              cccGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGAAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCTc          >  2:83067/1‑150 (MQ=255)
                                                                                                                                               ccgccgCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCTCa         >  2:240868/1‑150 (MQ=255)
                                                                                                                                                    gcAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCTCAGCGGa    >  2:733628/1‑150 (MQ=255)
                                                                                                                                                    gcAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCTCAGCGGa    >  1:1009230/1‑150 (MQ=255)
                                                                                                                                                      aGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCTCAGCGGAAt  <  1:1757132/150‑1 (MQ=255)
                                                                                                                                                      aGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCTCAGCGGAAt  >  1:969441/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCTCAGCGGAAT  >  NC_000913/1260755‑1261053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: