Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,311,285 A→C 9.2% E66A (GAG→GCG)  tonB → membrane spanning protein in TonB‑ExbB‑ExbD transport complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,311,2850AC 9.2% 277.7 / 15.8 106E66A (GAG→GCG) tonBmembrane spanning protein in TonB‑ExbB‑ExbD transport complex
Reads supporting (aligned to +/- strand):  ref base A (45/50);  new base C (3/7);  total (49/57)
Fisher's exact test for biased strand distribution p-value = 3.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.37e-01

Only 100 of 107 total aligned reads displayed.

GGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAA  >  NC_000913/1311136‑1311433
                                                                                                                                                     |                                                                                                                                                    
ggTCTGCATTCATGGTGCTGTTGTGGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGa                                                                                                                                                      >  2:48660/1‑150 (MQ=255)
  tCTGCATTCATGGTGCTGTTGTGGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGGGc                                                                                                                                                    >  2:1955389/1‑150 (MQ=255)
    tGCATTCATGGTGCTGTTGTGGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCg                                                                                                                                                  <  2:603641/150‑1 (MQ=255)
    tGCATTCATGGTGCTGTTGTGGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCg                                                                                                                                                  >  2:1652319/1‑150 (MQ=255)
      cATTCATGGTGCTGTTGTGGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCCGCCGCCACCGGAGCCggg                                                                                                                                                >  1:303183/1‑149 (MQ=255)
      cATTCATGGTGCTGTTGTGGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGACGATTTCTGTCACGATGGTTTCGACTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCggg                                                                                                                                                >  1:267193/1‑149 (MQ=255)
       aTTCATGGTGCTGTTGTGGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCggtg                                                                                                                                               >  2:1984118/1‑150 (MQ=255)
        ttCATGGTGCTGTTGTGGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCCAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCggtgg                                                                                                                                              <  2:1825443/150‑1 (MQ=255)
         tCATGGTGCTGTTGTGGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGATCTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCggtggt                                                                                                                                             <  1:1025709/150‑1 (MQ=255)
          cATGGTGCTGTTGTGGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCGGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGCTCCggtggcg                                                                                                                                            <  1:954363/150‑3 (MQ=255)
            tGGTGCTGTTGTGGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGCTCCggtggcggc                                                                                                                                          <  1:526170/150‑5 (MQ=255)
            tGGTGCTGTTGTGGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGCTCCggtggcggc                                                                                                                                          <  1:845072/150‑5 (MQ=255)
            tGGTGCTGTTGTGGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGCTCCggtggcggc                                                                                                                                          <  2:1862388/150‑5 (MQ=255)
            tGGTGCTGTTGGGGCGGGGCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGCTCCggtggcggc                                                                                                                                          <  1:1074294/150‑5 (MQ=255)
             ggTGCTGTTGTGGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTagag                                                                                                                                         >  2:497709/1‑150 (MQ=255)
              gTGCTGTTGTGGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGCTCCggtggcggctg                                                                                                                                        <  2:1483110/150‑7 (MQ=255)
               tGCTGTTGTGGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGcc                                                                                                                                       >  1:742230/1‑150 (MQ=255)
                  tgttgtGGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGa                                                                                                                                    >  2:1668551/1‑150 (MQ=255)
                     tgtgGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAAcc                                                                                                                                 >  2:2001613/1‑150 (MQ=255)
                         gCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGaa                                                                                                                             >  2:1487594/1‑150 (MQ=255)
                          cGGGTCTGCTCTCTCCCGCGGGACATCAGGTTATTCAACTACCTGCGCCGGCGCAGCCGCGTTCTGTCACGATGGTTACGCCTCCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGacc                                                                                                                            <  1:151854/150‑3 (MQ=255)
                           gcgTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGAATTCTGTCACGATGGTTACGCCTGCTGATCACGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGTAAcc                                                                                                                           <  1:1268442/148‑1 (MQ=255)
                                tGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGc                                                                                                                      <  1:897036/150‑1 (MQ=255)
                                 gCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGcc                                                                                                                     <  1:1467874/150‑1 (MQ=255)
                                 gCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGcc                                                                                                                     <  2:544135/150‑1 (MQ=255)
                                 gCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGACTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCTGAACCTGCGcc                                                                                                                     >  1:394334/1‑150 (MQ=255)
                                   tctATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGa                                                                                                                   >  1:1606940/1‑150 (MQ=255)
                                    ctATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGAt                                                                                                                  >  2:1925051/1‑150 (MQ=255)
                                      ataCCTCGGTACATCAGCCTATTCACCGCCCTGCGCCTGCGCAGCCGCTTTCTGCCCCGATCGTTCCGCCTCCTGTCATCGAACAGCCACAAGCCGTTCAGCGTCCACCGGAGCCTGTGGTAGAGCCAGAACCGGAACCTGAGCCGATcc                                                                                                                <  2:83453/150‑1 (MQ=255)
                                        aCCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATcccc                                                                                                              <  1:1607632/150‑1 (MQ=255)
                                         ccTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATccccc                                                                                                             >  2:1801790/1‑149 (MQ=255)
                                          cTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGCGCCGATCCCCgc                                                                                                            >  1:1897811/1‑149 (MQ=255)
                                            cGGTACTTCAGGTTAATGAACTTCCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAAc                                                                                                          <  1:1057937/150‑1 (MQ=255)
                                                aCATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGCACCGcc                                                                                                      >  1:472802/1‑150 (MQ=255)
                                                    cAGGTTATTGAACTCCCTGCGCCTGCGCAGCCGAGTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGACGTTCAGCCGCCACCGGCGCGGGTGGTAGAGCCAGAACAGGACCCTGAGCCGATCCCCGCACCGCCaaac                                                                                                  >  2:1906191/1‑149 (MQ=255)
                                                    cAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCaaaa                                                                                                  >  1:918688/1‑150 (MQ=255)
                                                     aGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAGCCTGAGCCGATCCCCGAACCGCCAAaag                                                                                                 >  1:753982/1‑150 (MQ=255)
                                                     aGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTCTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAAACTGAGCCGATCCCCGAACCGCCAAaag                                                                                                 <  2:1295709/150‑1 (MQ=255)
                                                      ggTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAaaga                                                                                                <  2:1465838/150‑1 (MQ=255)
                                                       gTTATTGAACTACCTGCTCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAaagaa                                                                                               <  2:1561959/150‑1 (MQ=255)
                                                        ttATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAaagaag                                                                                              <  1:605560/150‑1 (MQ=255)
                                                             gAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCg                                                                                         >  2:1690434/1‑150 (MQ=255)
                                                               aCTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCggt                                                                                       >  1:700043/1‑150 (MQ=255)
                                                                cTACCTGCGCCTGAGCAGCCGATTTCTGTCCCGCTGGTTCCGCCTGCTCATCTCGACCCGCCACAAGCCGTTCAGCCGCCACCGGCGCCGGTGGTAGAGCCAGGCCCGGACCCTGAGCCGATCCCCGAGCCGCCAAAAGACGCACCggtg                                                                                      >  1:1155446/1‑150 (MQ=255)
                                                                 tccctgcgcctgcgCAGCCGATTTCTGTCCCGCTGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGACACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCggtgg                                                                                     <  2:363010/148‑1 (MQ=255)
                                                                   cctgcgcctgcgCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCCGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGCTCCCCGAACCGCCAAAAGAAGCACCGGGGGTc                                                                                   >  1:1482001/1‑150 (MQ=255)
                                                                   cctgcgcctgcgCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATATCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTc                                                                                   <  2:84985/150‑1 (MQ=255)
                                                                    ctgcgcctgcgcAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCCCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCa                                                                                  <  1:1321947/150‑1 (MQ=255)
                                                                       cgcctgcgcAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTg                                                                               >  1:631247/1‑150 (MQ=255)
                                                                         cctgcgcAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGaa                                                                             <  1:708441/150‑1 (MQ=255)
                                                                          ctgcgcAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGaaa                                                                            <  2:1378802/150‑1 (MQ=255)
                                                                            gcgcAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGCTCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAaag                                                                          >  2:1666735/1‑150 (MQ=255)
                                                                            gcgcAGCCGATTTCTGTCACGATGGGTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAaag                                                                          <  1:607737/150‑1 (MQ=255)
                                                                              gcAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAaagcc                                                                        >  1:913563/1‑150 (MQ=255)
                                                                                  ccGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAaagccgaag                                                                    >  1:1098286/1‑150 (MQ=255)
                                                                                   cGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGCGCCGATCCCCGAACCGCCAAAAGAAGCCCCGGTGGTCATTGAAaagccgaagc                                                                   >  2:1666016/1‑150 (MQ=255)
                                                                                   cGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAaagccgaagc                                                                   <  2:971892/150‑1 (MQ=255)
                                                                                    gATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAaagccgaagcc                                                                  <  1:1796318/150‑1 (MQ=255)
                                                                                         cTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGTAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAAc                                                             <  1:497709/150‑1 (MQ=255)
                                                                                          tGTCCCGCTGGATCCGCCTGATGAGCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAAcc                                                            <  2:295619/150‑1 (MQ=255)
                                                                                           gTCACGATGGTTACGCCTGCTGCTCTCGAACCGCCACAAGCCGTTCAGCCGCCCCCGGAGCCGGTGGTAGCGGCAGACCCCTCACCTGAGCCGATCCCCGATCCGGCATAAGAAGCCCCGGTGGGCCGTGAAGAGCAGAAGCGGAAAccg                                                           >  2:934400/1‑149 (MQ=255)
                                                                                           gTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGACCCGGAACCTGAGCCGATCCCCGACCCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAACCCt                                                           >  1:503900/1‑150 (MQ=255)
                                                                                             cACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTaa                                                         >  1:888533/1‑150 (MQ=255)
                                                                                              aCGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTGCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAg                                                        <  2:843028/150‑1 (MQ=255)
                                                                                               cGAGGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGc                                                       <  2:1332546/150‑1 (MQ=255)
                                                                                                gATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGcc                                                      >  2:1617791/1‑150 (MQ=255)
                                                                                                gATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGcc                                                      >  2:177637/1‑150 (MQ=255)
                                                                                                   ggTTACGCCTGCTGAGCGCGAACCGCCACAAGCCGTGCCGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGCACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCaaa                                                   <  2:1264638/150‑1 (MQ=255)
                                                                                                    gTTTCGCCTGCTGATCTCGAACCGCCACAAGCCGGTCAGCCGACACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCaaac                                                  <  1:1006129/150‑2 (MQ=255)
                                                                                                     ttACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAAc                                                 <  1:1643704/150‑1 (MQ=255)
                                                                                                       aCGCGTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCACCAAAAGAAGCACCGGTGGTCATTGAAAAGCCTAAGCCGAAACCTAAGCCAAAACCg                                               <  1:1038452/150‑1 (MQ=255)
                                                                                                        cGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGATCCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGa                                              >  1:1086946/1‑150 (MQ=255)
                                                                                                        cGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGACCCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGACGCGTCCTAAGACAAAACCGa                                              >  1:1518624/1‑150 (MQ=255)
                                                                                                            tgctgATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGcc                                          <  1:1567715/150‑1 (MQ=255)
                                                                                                             gctgATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGACGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCg                                         <  1:769948/150‑1 (MQ=255)
                                                                                                             gctgATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGACGCCg                                         >  1:1926311/1‑150 (MQ=255)
                                                                                                                gATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGATGCCgggg                                      >  2:1664478/1‑148 (MQ=255)
                                                                                                                  tctcGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGAAGAGCCAGAACCGGAAGCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAGGCCGCAGCCGCAACCTAAGCCCAATCCGCAGCCGGGGaa                                    >  1:88268/1‑150 (MQ=255)
                                                                                                                     cGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGAGGTAGAGCCAGAACCGGAACCTGAGCCGCTCCCCCAACCGCCACACGAAGCACCGGTGCTCATTGAAACGCCGACGCCGACCCCTAAGCCAAAACCGAAGCCGGTGaaaaa                                 >  1:381166/1‑150 (MQ=255)
                                                                                                                      gAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAg                                <  1:1288372/150‑1 (MQ=255)
                                                                                                                       aaCCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAgg                               <  1:995178/150‑1 (MQ=255)
                                                                                                                        aCCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGACCCTGCGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGt                              >  2:1514808/1‑150 (MQ=255)
                                                                                                                        aCCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGt                              <  1:1668551/150‑1 (MQ=255)
                                                                                                                         ccGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTa                             <  1:1801790/150‑1 (MQ=255)
                                                                                                                            ccACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAg                          <  1:1984118/150‑1 (MQ=255)
                                                                                                                                 aGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGagca                     >  2:593599/1‑150 (MQ=255)
                                                                                                                                    cGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGGAAAAGGTACAGGAGCAGcc                  >  2:862171/1‑150 (MQ=255)
                                                                                                                                     gTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACCGGAGCAGCCa                 >  1:236872/1‑150 (MQ=255)
                                                                                                                                     gTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCa                 <  2:742230/150‑1 (MQ=255)
                                                                                                                                         ctctaccACCGGCTCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGCGCAGCCAAAAc             >  2:1945061/6‑150 (MQ=255)
                                                                                                                                          gccgtcACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGACCCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAAcg            >  2:1697789/1‑150 (MQ=255)
                                                                                                                                           ccgccACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAAcgc           >  2:1678423/1‑150 (MQ=255)
                                                                                                                                            cgccACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAATAAGCACCGGTGGTAATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCTAAGCCGGTGAAAAAGGTACGGGAGCAGCCAAAAcgcg          <  2:394334/150‑1 (MQ=255)
                                                                                                                                            cgccACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAAcgcg          <  2:753982/150‑1 (MQ=255)
                                                                                                                                            cgccACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAAcgcg          <  2:1219762/150‑1 (MQ=255)
                                                                                                                                             gctatcGGAGCCGGTGTTAGAGACAGAACAGGAACCTGAGCCGATCCCCGAACCGCCAAAAGATGCACCGGTGGTCATTGAAAAGACGAAGCCGAAACATAAGCCAAAACCGCAGACGGTGAAAAAGTTTCAGGAGCAGCCAAAACGCGa         <  2:401106/145‑1 (MQ=255)
                                                                                                                                              ccACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCTCCAAAAGAAGCACCGTTGGTCATTGAAAAGCCGAAGCCGAAACCTAATCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGAt        <  1:587733/150‑1 (MQ=255)
                                                                                                                                                   ggAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCaa   <  2:588444/150‑1 (MQ=255)
                                                                                                                                                    gAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCaaa  <  1:48660/150‑1 (MQ=255)
                                                                                                                                                    gAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCaaa  <  1:2001613/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTGCTCTATACCTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTTACGCCTGCTGATCTCGAACCGCCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAGCCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAGCCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAA  >  NC_000913/1311136‑1311433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: