Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 114,506 G→T 5.3% intergenic (+19/+16) guaC → / ← hofC GMP reductase/assembly protein in type IV pilin biogenesis, transmembrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913114,5060GT 5.3% 419.2 / 9.9 141intergenic (+19/+16)guaC/hofCGMP reductase/assembly protein in type IV pilin biogenesis, transmembrane protein
Reads supporting (aligned to +/- strand):  ref base G (68/65);  new base T (4/4);  total (72/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.09e-01

Only 100 of 145 total aligned reads displayed.

CGCGAGATATTTTGGGCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGCCAGCG  >  NC_000913/114357‑114655
                                                                                                                                                     |                                                                                                                                                     
cgcgAGATATTTTGGGCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTgg                                                                                                                                                       >  1:1567182/1‑150 (MQ=255)
cgcgAGATATTTTGGGCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTgg                                                                                                                                                       >  1:524563/1‑150 (MQ=255)
  cgAGATATTTTGGGCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTCCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAg                                                                                                                                                     >  2:1885053/1‑150 (MQ=255)
   gagaTATTTTGGGCTGCCGGCGTTCAGCTTGTACATACGTTGGGGCTTCATGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAATTAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGc                                                                                                                                                    <  2:95816/150‑1 (MQ=255)
   gagaTATTTTGGGCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGc                                                                                                                                                    <  2:1181478/150‑1 (MQ=255)
      atatTTTGGGCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGAAATCTCCCAACGCTGGCGTGGAGCAac                                                                                                                                                 <  2:1459963/150‑1 (MQ=255)
       tatTTTGGGAGGCCTTCGTTAAGCTTGTACATACGTTGGGGCTTCACGCATGAAATAGCTGAACAAGCGCACCACGTTTATTGGTGTGCAGGAACAAGAAAACCGCATATTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGAAaca                                                                                                                                                <  2:134096/150‑1 (MQ=255)
        atTTTGGGCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATATCCCAACGCTGGCGAGGAGcaccac                                                                                                                                               >  2:167586/1‑150 (MQ=255)
           ttGGGCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGcc                                                                                                                                            >  1:942032/1‑150 (MQ=255)
             gggCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAg                                                                                                                                          <  1:255786/150‑1 (MQ=255)
              ggcggcCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACACGAAAACTGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGTTGCTCCACGCCAGc                                                                                                                                         <  2:1026030/150‑1 (MQ=255)
               gcggcCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCg                                                                                                                                        <  2:1849473/150‑1 (MQ=255)
               gcggcCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCg                                                                                                                                        <  2:1574663/150‑1 (MQ=255)
               ccggcCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCGGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCg                                                                                                                                        >  1:1334938/2‑150 (MQ=255)
               ccggcCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCACCGCTGGCGTGGAGCAACACGCCAGCg                                                                                                                                        <  2:491973/149‑1 (MQ=255)
                  gcCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGTTGCTCCACGCCAGCGttg                                                                                                                                     <  1:752757/150‑2 (MQ=255)
                    cTGCGTTCATCTTGTACATACGTTGGGGCTTCACGCCTGAAAGACCTGACCAAGCGCACCACGTTTATTCGTGTGCAGTAACAAGAAAACCGCATCTTCAACAACCTGTAAGCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTatc                                                                                                                                   <  1:1015799/150‑1 (MQ=255)
                     tGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTatcc                                                                                                                                  <  2:1308050/150‑1 (MQ=255)
                     tGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTatcc                                                                                                                                  >  1:32131/1‑150 (MQ=255)
                           cAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGTGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTatcccatccc                                                                                                                            <  1:902887/150‑1 (MQ=255)
                           cAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTatcccatccc                                                                                                                            <  2:785880/150‑1 (MQ=255)
                              cTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCAACCCCCt                                                                                                                         >  1:175462/1‑150 (MQ=255)
                                tGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACATGAAATCTCCCAACGCTGGCGTGGATCAACACTCCAGCGTTATCCCAACCCACTca                                                                                                                       >  2:1525217/1‑150 (MQ=255)
                                   aCATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTcatcg                                                                                                                    <  1:1170269/150‑1 (MQ=255)
                                    cATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTcatcgc                                                                                                                   >  2:1519422/1‑150 (MQ=255)
                                     aTACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTcatcgca                                                                                                                  <  1:1047964/150‑1 (MQ=255)
                                       aCGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTcatcgcatc                                                                                                                <  1:1722704/150‑1 (MQ=255)
                                        cGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTcatcgcatcg                                                                                                               <  1:1022043/150‑1 (MQ=255)
                                        cGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCGTCACCAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGGTATCCCATCACACTcatcgcatcg                                                                                                               >  1:1056697/1‑150 (MQ=255)
                                          ttGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGcc                                                                                                             <  1:507461/150‑1 (MQ=255)
                                               gCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAAt                                                                                                        >  1:764650/1‑150 (MQ=255)
                                                cTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATg                                                                                                       <  2:654760/150‑1 (MQ=255)
                                                cTTCACGCCTGAAAGAGCTGACCAAGCGCACAACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATAGTCAACAACCAGTAATCTCCCAACGCTGGCGTGGAGCAACAAGCCAGCGTTAACACATCCCACTCATAGCATCGCCAAAATg                                                                                                       >  2:524923/1‑150 (MQ=255)
                                                 ttCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTTTCCCATCCCCCTCATCGCATCGCCTAAATgg                                                                                                      >  1:1003817/1‑150 (MQ=255)
                                                  tCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAACACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGAGGAGCAACACGCCAGCGTTATCCCCTCCCACTCCTCGCATCGCCTAAATGGa                                                                                                     >  1:79516/1‑150 (MQ=255)
                                                   cACGCCTGAAAGAGCGGACCAAGCGCACCCCGTTTATTCGGGTGCAGGAACAAGAAAACCGCCTCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGaa                                                                                                    <  2:1312078/150‑1 (MQ=255)
                                                      gCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACCACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAAt                                                                                                 >  1:1166336/1‑150 (MQ=255)
                                                      gCCTGAAAGAGCTGACCAAGCGCACCACGTTTAATCGTGTGAAGGAACAAGAAACCCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGCGCAACACGCCAGCGTTAACCCCACCCACTCCTCGCCTCGCCTAAATGGAAAAt                                                                                                 >  1:1889682/1‑150 (MQ=255)
                                                       ccTGTAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAAtt                                                                                                >  2:907276/1‑150 (MQ=255)
                                                         tGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTgg                                                                                              >  2:2010851/1‑150 (MQ=255)
                                                          gAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTggg                                                                                             >  2:1060801/1‑149 (MQ=255)
                                                           aaaGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCa                                                                                            <  2:416632/150‑1 (MQ=255)
                                                            aaGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATTGAAAATTGGCAg                                                                                           <  2:1198456/150‑1 (MQ=255)
                                                            aaGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAg                                                                                           >  2:1796715/1‑150 (MQ=255)
                                                              gagCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGAt                                                                                         <  2:1598821/150‑1 (MQ=255)
                                                              gagCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGAt                                                                                         <  1:56173/150‑1 (MQ=255)
                                                                 cTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACCACCTGTAATCTCCCACCGCTCGCGTGGAGCAACACGCCAGCGTTAACCCCTCCCACTCATCGCATCGCCTAAATGGAAACTTGGCAGATACa                                                                                      >  2:262039/1‑150 (MQ=255)
                                                                     ccAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGc                                                                                  >  1:1182500/1‑150 (MQ=255)
                                                                      cAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGcc                                                                                 >  2:1215598/1‑150 (MQ=255)
                                                                       aaGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGccc                                                                                >  2:1971136/1‑149 (MQ=255)
                                                                       aaGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGcca                                                                                <  2:603296/150‑1 (MQ=255)
                                                                           gcACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAGTCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGccaccac                                                                            <  1:1744731/150‑1 (MQ=255)
                                                                            caccacGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGccaccacc                                                                           <  2:1445037/150‑1 (MQ=255)
                                                                             accacGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGccaccaccc                                                                          >  2:1212687/1‑149 (MQ=255)
                                                                              ccacGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAg                                                                         >  1:513132/1‑150 (MQ=255)
                                                                               cacGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGc                                                                        <  2:1339140/150‑1 (MQ=255)
                                                                                         cGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCaataa                                                              <  2:1485566/150‑1 (MQ=255)
                                                                                         cGTGTGCAGGAACAAGAAAACCGCATCTTAAACAACCTGTAATCTCCCAACGCAGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCAACGCATCCCCTAAATGGAAAATTGGCAGATACATTGCCACAACCAGCGTACCaataa                                                              >  2:440633/1‑150 (MQ=255)
                                                                                            gtgCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATtc                                                           >  1:11873/1‑150 (MQ=255)
                                                                                            gtgCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACAAATAATtc                                                           >  1:798656/1‑150 (MQ=255)
                                                                                             tgCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATtcc                                                          <  1:1795732/150‑1 (MQ=255)
                                                                                               cAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATtcctc                                                        <  1:562109/150‑1 (MQ=255)
                                                                                               cAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATtcctc                                                        <  1:987316/150‑1 (MQ=255)
                                                                                               cAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATtcctc                                                        >  1:897744/1‑150 (MQ=255)
                                                                                                 ggAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAAGAATTCCTccc                                                      >  1:742902/1‑150 (MQ=255)
                                                                                                  gAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCg                                                     >  1:991473/1‑150 (MQ=255)
                                                                                                  gAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGACCAACACGCAAGCGTTATACCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATAAATTTCCACCACCAGCGTACCAATAATTCCTCCCg                                                     >  1:1817585/1‑150 (MQ=255)
                                                                                                     cAAGAAAACCGCTTCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCAGCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGGTa                                                  >  2:1483503/1‑150 (MQ=255)
                                                                                                     cAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCCTCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAAGAATTCCTCCCGtga                                                  >  1:93582/1‑148 (MQ=255)
                                                                                                      aaGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCGAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTAt                                                 >  2:1080751/1‑150 (MQ=255)
                                                                                                      aaGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCCACGCATCCCCTAAATGGAAACTTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTAAt                                                 >  1:136190/1‑150 (MQ=255)
                                                                                                       aGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCTATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTAtt                                                >  1:483470/1‑149 (MQ=255)
                                                                                                        gAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCGAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGa                                               <  1:628204/150‑1 (MQ=255)
                                                                                                        gAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGa                                               >  1:730473/1‑150 (MQ=255)
                                                                                                           aaCCGCATCTGCCACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCa                                            <  2:1056091/150‑1 (MQ=255)
                                                                                                            aCCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTCGCAGATACAATGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAg                                           >  1:1300587/1‑150 (MQ=255)
                                                                                                             ccGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCGACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGc                                          <  2:1288500/150‑1 (MQ=255)
                                                                                                             ccGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGc                                          <  1:919707/150‑1 (MQ=255)
                                                                                                                    ttCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCg                                   >  1:1570041/1‑150 (MQ=255)
                                                                                                                    ttCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCACCCGTTATGATCAGCAACGCCg                                   <  2:834894/150‑1 (MQ=255)
                                                                                                                     tCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATTCATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCgg                                  >  1:670966/1‑150 (MQ=255)
                                                                                                                        acaacCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCAGTTATGATCAGCAACGCCGGTTc                               >  2:720049/1‑150 (MQ=255)
                                                                                                                         caacCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTcc                              >  1:1826636/1‑150 (MQ=255)
                                                                                                                          aacCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCa                             >  2:1478648/1‑150 (MQ=255)
                                                                                                                           acCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGTAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAg                            >  1:147004/1‑150 (MQ=255)
                                                                                                                           acCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAAATGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAg                            <  1:1455830/150‑1 (MQ=255)
                                                                                                                            ccTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGt                           >  2:862923/1‑150 (MQ=255)
                                                                                                                                tAATCTCCCAACGCTGGCGTTGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAgg                       <  1:1885053/150‑1 (MQ=255)
                                                                                                                                 aaTCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTCCCAATAATTCCTCCCGTTATGATCAGGAACGCCGGTTCCAGTACGGc                      >  1:152156/1‑150 (MQ=255)
                                                                                                                                   tctcCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTg                    <  1:1685068/150‑1 (MQ=255)
                                                                                                                                     ggataaCGCTGGCGTGTTGCTCCACGCCAGCGTTATCCCTTCCAACTCATCGCATCGGCTACATGGTAAATTGGCTGAGACATTGCCACCACCCGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCg                  >  1:374470/5‑150 (MQ=255)
                                                                                                                                        catctcTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTACATGGAAAATTGGCAGATACCTTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCCACGCCGGTTCCAGTAAGGCTGCGAgg               <  1:71916/145‑1 (MQ=255)
                                                                                                                                         ttcGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCGCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGt              <  1:167586/148‑1 (MQ=255)
                                                                                                                                         aaCGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCCTCGCCTAAATGGAAAATTGGCAGATACATTGCCACCCCCAGCGTCCCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGt              >  1:1769908/1‑150 (MQ=255)
                                                                                                                                           cGCTGGCGTGTTGCTCCACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGttt            >  2:1642651/1‑149 (MQ=255)
                                                                                                                                            gCTGGCGTGTTGCTCCACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTCCCAATAATTCCTCCCGTTATGATCAGCACCGCCGGTTCCAGTAAGGCTGCGAGGTTAt           >  1:1311668/1‑150 (MQ=255)
                                                                                                                                             cTGGCGTGTTGCTCCACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTTTGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATc          >  2:1755572/1‑150 (MQ=255)
                                                                                                                                              tGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATcc         <  2:510153/150‑1 (MQ=255)
                                                                                                                                              tGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATcc         <  2:1830872/150‑1 (MQ=255)
                                                                                                                                                   tGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATAAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGCCAg    >  2:227946/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGCGAGATATTTTGGGCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGCCAGCG  >  NC_000913/114357‑114655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: