Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,410,990 T→A 5.8% intergenic (+106/+23) dbpA → / ← ttcA ATP‑dependent RNA helicase, specific for 23S rRNA/tRNA s(2)C32 thioltransferase, iron sulfur cluster protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,410,9900TA 5.8% 415.7 / 11.3 148intergenic (+106/+23)dbpA/ttcAATP‑dependent RNA helicase, specific for 23S rRNA/tRNA s(2)C32 thioltransferase, iron sulfur cluster protein
Reads supporting (aligned to +/- strand):  ref base T (73/65);  new base A (5/4);  total (78/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.70e-01

Only 100 of 149 total aligned reads displayed.

GGAAGATTAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCAC  >  NC_000913/1410841‑1411138
                                                                                                                                                     |                                                                                                                                                    
ggAAGATTAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCt                                                                                                                                                      >  2:1388610/1‑150 (MQ=255)
   aGATTAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTgc                                                                                                                                                   <  2:1493142/150‑1 (MQ=255)
    taTTAAATGAATATCGTGCCTGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGTAAGATTAAAAATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTgcg                                                                                                                                                  <  1:964138/149‑1 (MQ=255)
    gATTAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTgcg                                                                                                                                                  >  2:932827/1‑150 (MQ=255)
    gATTAAAGGAAAAACGTGCCGGGTGCGGTTATAAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGATGGCCGCGTCTTATCCGGCCTCCATAAGATTAGCCCGATACGCTTgcg                                                                                                                                                  >  1:1005131/1‑150 (MQ=255)
      ttAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCa                                                                                                                                                >  1:1153110/1‑150 (MQ=255)
      ttAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCa                                                                                                                                                >  1:1310101/1‑150 (MQ=255)
            gAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAATATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGgc                                                                                                                                          >  2:211125/1‑150 (MQ=255)
            gAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGGGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCTGgc                                                                                                                                          <  2:1330390/150‑1 (MQ=255)
            gAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATGCGCAAGCGTATCGGgc                                                                                                                                          <  2:184128/150‑1 (MQ=255)
              aaaaCGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATGCGCAAGCGTATCGGgcta                                                                                                                                        <  1:611971/150‑3 (MQ=255)
               aaaCGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATGCGCAAGCGTATCGGgctac                                                                                                                                       <  1:405946/150‑4 (MQ=255)
                  cgggacGGGGGAGGTTAGTAAATTAATGAAATGTTGAATTGCCGGGTTCAAGAGTACACATCTTATTCGGGCTTGCCGTATGCGACGCTGGCCGCGTCGAATCCGGCCTCAATACGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATc                                                                                                                                    <  2:1276406/145‑1 (MQ=255)
                   gTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATcc                                                                                                                                   <  1:1577803/150‑1 (MQ=255)
                     gCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTg                                                                                                                                 >  2:1400962/1‑150 (MQ=255)
                      ccGGGTGCGGTTATTAAAATAATAAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGGCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTgg                                                                                                                                <  1:1200952/150‑1 (MQ=255)
                       cGGGTGCGGTTATTAAAATAATGAAATGGTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGCGATGGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTggc                                                                                                                               <  1:1866404/150‑2 (MQ=255)
                         ggTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTAATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTa                                                                                                                             <  1:280396/150‑1 (MQ=255)
                                 tattaAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACtt                                                                                                                     <  1:879572/150‑1 (MQ=255)
                                 tattaAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACtt                                                                                                                     >  1:720111/1‑150 (MQ=255)
                                 tattaAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACtt                                                                                                                     <  1:643128/150‑1 (MQ=255)
                                   ttaAAATAATGAAATGTTGAATTGCAGCGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCa                                                                                                                   <  2:1215924/150‑1 (MQ=255)
                                    taAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCaa                                                                                                                  >  1:1587147/1‑150 (MQ=255)
                                     aaaaTAATGAAATGTTGAATTGCCGGGTGCAAGAGTTAACATCTTATTCGGGATTGCCGGATGCTACGCTGGCATCGTCTTATCAGGCCTCCATAAGAGTAGCCCGAAACGCTTGCGCATCGGGCGCTATCCAGGTTATTTCACTTCAAc                                                                                                                 >  1:118467/1‑150 (MQ=255)
                                     aaaaTAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAAc                                                                                                                 >  2:212538/1‑150 (MQ=255)
                                     aaaaTAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAAc                                                                                                                 >  1:938359/1‑150 (MQ=255)
                                     aaaaTAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAAc                                                                                                                 <  1:761033/150‑1 (MQ=255)
                                     aaaaTAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGAAACGCTAGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAAc                                                                                                                 >  2:1874599/1‑150 (MQ=255)
                                       aataatGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACca                                                                                                               <  2:1946894/150‑1 (MQ=255)
                                           atGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACAtt                                                                                                           >  1:266963/1‑150 (MQ=255)
                                              aaaTGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAg                                                                                                        <  1:346749/150‑1 (MQ=255)
                                                  gtggAATTGCCGGGGGCAAGAGTAAACATCTTATTCGGGACTGCCGCATCCGACGCTGGCCGCGTCTTCTCCGGCCTCCATAAGAGTCGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGGAATTTCACTTCAACCACATTCAGCCGt                                                                                                    <  2:307492/147‑1 (MQ=255)
                                                  gTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGt                                                                                                    >  1:458629/1‑150 (MQ=255)
                                                    tGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTAACTTAAAACACATTCAGCCGTaa                                                                                                  >  2:1682097/1‑150 (MQ=255)
                                                     gAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAAc                                                                                                 <  1:1769711/150‑1 (MQ=255)
                                                        ttGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCa                                                                                              <  1:515055/150‑1 (MQ=255)
                                                         tGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGTCGCGGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCAt                                                                                             <  2:519476/150‑1 (MQ=255)
                                                          gCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTACACTTCAACCACATTCCGCCGTAACTCATc                                                                                            >  2:1488123/1‑150 (MQ=255)
                                                                  cAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGAt                                                                                    >  2:833587/1‑150 (MQ=255)
                                                                   aaGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGACGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCTGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCAACCAACAGAtt                                                                                   >  1:248533/1‑150 (MQ=255)
                                                                    agagTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGAttt                                                                                  >  2:361396/1‑150 (MQ=255)
                                                                       gTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCa                                                                               >  2:1241876/1‑150 (MQ=255)
                                                                            cagcgtATTCGGGATTGCCGGAAGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCAtcttc                                                                          <  2:375796/145‑1 (MQ=255)
                                                                             atgatATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGACTAGCCCGATCCGCGTGCGCATCGGGCGCTATCCTGGTTATATCACTTCAACCACATTCAGCCGGAACTCATCCAACAGATTTTCAtcttct                                                                         <  2:1144638/146‑1 (MQ=255)
                                                                             aTCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCAtcttct                                                                         >  2:1895072/1‑150 (MQ=255)
                                                                              tCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCAtcttctt                                                                        >  2:585949/1‑150 (MQ=255)
                                                                               cTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCAtcttcttc                                                                       <  2:1897821/150‑1 (MQ=255)
                                                                               cTTATTCGGGATTGCCGGATGCGACGCTGGCAGCGTCTTATCCGTCCTCCATAAGAGTAGCCCGATACTCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCTTAACTCATCCAACTGATTTTCAtcttcttc                                                                       <  1:1466947/150‑1 (MQ=255)
                                                                                 tATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTg                                                                     <  2:545461/150‑1 (MQ=255)
                                                                                  aTTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTgg                                                                    >  1:1594762/1‑150 (MQ=255)
                                                                                  aTTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATACGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTAGCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCACCTGATTTTCAACTTCTTCTgg                                                                    >  2:1942298/1‑150 (MQ=255)
                                                                                       ggATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGcc                                                               <  1:1378139/150‑1 (MQ=255)
                                                                                        gATTGCCGGATGCGACACTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCa                                                              >  2:1128282/1‑150 (MQ=255)
                                                                                           ttcaggATGCGACGCTGGCCGCGTCTTATCCGGCCTCCAAAAGAGTAGCCCGATACGCTTGCTCATCTGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCa                                                           <  2:1234468/146‑1 (MQ=255)
                                                                                               ggATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGcc                                                       <  1:764686/150‑1 (MQ=255)
                                                                                               ggATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGcc                                                       <  1:1241876/150‑1 (MQ=255)
                                                                                               ggATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGGTATTTGACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGAACGcc                                                       >  2:746231/1‑150 (MQ=255)
                                                                                                    ggACGCTGGCCGCGTCTTATCCGGCCTCAATAAGAGTAGCCCTATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTg                                                  <  1:1406679/149‑1 (MQ=255)
                                                                                                    cGACGCTGGACGCGTCTTATCCGGCCTCAATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGGAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTg                                                  <  1:1399696/150‑1 (MQ=255)
                                                                                                     gACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTAAACCACATTCAGCCGTAACTCATCCAACAGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGgtgt                                                 >  1:1689433/1‑150 (MQ=255)
                                                                                                       cGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCCACTGATCTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAg                                               >  1:1670230/1‑150 (MQ=255)
                                                                                                         cTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGGTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTg                                             <  2:243120/150‑1 (MQ=255)
                                                                                                         cTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTg                                             <  2:1153110/150‑1 (MQ=255)
                                                                                                         cTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTg                                             <  1:754389/150‑1 (MQ=255)
                                                                                                         cTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTg                                             >  1:1606995/1‑150 (MQ=255)
                                                                                                          tGGCCGCGTCTTATCCGTCCTCAATAAGAGTAGCCGTATACGTTTGGGCATGGGGGGCTATCCTGGTTATTTCACTTCAACCACTTTCAGCCTTAACTCATCCAACTTATTTTCTTCTTCTTCTGGCTGCCAGCACGCCGGTTGTTGTgg                                            <  2:1005131/150‑1 (MQ=255)
                                                                                                            gCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGa                                          <  1:360542/150‑1 (MQ=255)
                                                                                                            gCCGCGTCGTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGa                                          <  1:1453523/150‑1 (MQ=255)
                                                                                                             ccGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGAt                                         >  1:1880308/1‑150 (MQ=255)
                                                                                                                 gTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTCGCTGCCAGCACGCCGGTTGTAGAGGGATctct                                     >  1:663163/1‑150 (MQ=255)
                                                                                                                 gTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTGGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTAGGATctct                                     <  2:255823/150‑1 (MQ=255)
                                                                                                                   cTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCACATGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTc                                   >  2:1351450/1‑150 (MQ=255)
                                                                                                                   cTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTc                                   >  2:282097/1‑150 (MQ=255)
                                                                                                                   cTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCAGCTTCTACTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTc                                   >  1:523273/1‑150 (MQ=255)
                                                                                                                       tCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCCACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGa                               >  1:1080147/1‑150 (MQ=255)
                                                                                                                        ccGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGAt                              <  1:398472/150‑1 (MQ=255)
                                                                                                                        ccGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGAt                              >  1:202604/1‑150 (MQ=255)
                                                                                                                         cGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATc                             <  1:1147517/150‑1 (MQ=255)
                                                                                                                         cGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATc                             >  2:1078532/1‑150 (MQ=255)
                                                                                                                          ggCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCa                            <  1:206705/150‑1 (MQ=255)
                                                                                                                              tCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTATTCGCGATCAAAcc                        >  2:694167/1‑149 (MQ=255)
                                                                                                                              tCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTAATTCACTTCAACCACATTCAGCCGTAACCCATCCAACTGATTTTCATCTTCTTCAGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACg                        <  1:1639134/150‑1 (MQ=255)
                                                                                                                               ccATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGAACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGc                       >  1:1533819/1‑150 (MQ=255)
                                                                                                                                  tAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAg                    <  2:938359/150‑1 (MQ=255)
                                                                                                                                    agagTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGAt                  >  2:1636184/1‑150 (MQ=255)
                                                                                                                                     cagTCGCCCGCTACGCTTGCGAATCGGGCGCTATCCTGGATATATCACTTCAACCACAAGCAGCCGTAACTCCTCCAACTGATATTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGAAGTGGGATCTCTTCTCGATCAAACTCCAGATc                 <  1:284377/149‑1 (MQ=255)
                                                                                                                                     atagcgCCCGATGCGCAAGCGTATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATc                 >  2:587704/6‑150 (MQ=255)
                                                                                                                                      agTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCa                >  2:1026785/1‑150 (MQ=255)
                                                                                                                                         aGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCAccc             >  1:734910/1‑150 (MQ=255)
                                                                                                                                         aGCCCGATACGCTTGCGCATCGGGCGATATCCTGGTTATTACACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTTCCACCACGCCGGTTGTAGTGGGATCTATTCGCGATCAACCGCCAGATCAccc             >  2:67902/1‑150 (MQ=255)
                                                                                                                                          gCCCGATGCGCAAGCGTATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCAcccc            >  1:568620/1‑150 (MQ=255)
                                                                                                                                          gCCCGATGCGCAAGCGTATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCAcccc            >  1:1820511/1‑150 (MQ=255)
                                                                                                                                          gCCCGATGCGCAAGCGTATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCAcccc            >  1:1055744/1‑150 (MQ=255)
                                                                                                                                            ccGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCg          >  1:667295/1‑150 (MQ=255)
                                                                                                                                              gATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGtt        <  1:578665/150‑1 (MQ=255)
                                                                                                                                              gATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGtt        <  1:1182580/150‑1 (MQ=255)
                                                                                                                                                tACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTaa      <  1:542213/150‑1 (MQ=255)
                                                                                                                                                  cGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAAcc    <  1:977054/150‑1 (MQ=255)
                                                                                                                                                    cTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTACCTCATCCAACTGATTTTCAGCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATATCTTCGCGATCAAACGCCAGATCACCCCCGGTAACCAc  >  2:1269558/1‑150 (MQ=255)
                                                                                                                                                    cTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCAc  >  2:1197056/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GGAAGATTAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCAC  >  NC_000913/1410841‑1411138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: