Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,410,991 T→A 5.8% intergenic (+107/+22) dbpA → / ← ttcA ATP‑dependent RNA helicase, specific for 23S rRNA/tRNA s(2)C32 thioltransferase, iron sulfur cluster protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,410,9910TA 5.8% 422.6 / 11.3 148intergenic (+107/+22)dbpA/ttcAATP‑dependent RNA helicase, specific for 23S rRNA/tRNA s(2)C32 thioltransferase, iron sulfur cluster protein
Reads supporting (aligned to +/- strand):  ref base T (73/65);  new base A (5/4);  total (78/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.88e-01

Only 100 of 149 total aligned reads displayed.

AGATTAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTT  >  NC_000913/1410844‑1411140
                                                                                                                                                   |                                                                                                                                                     
aGATTAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTgc                                                                                                                                                     <  2:1493142/150‑1 (MQ=255)
 taTTAAATGAATATCGTGCCTGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGTAAGATTAAAAATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTgcg                                                                                                                                                    <  1:964138/149‑1 (MQ=255)
 gATTAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTgcg                                                                                                                                                    >  2:932827/1‑150 (MQ=255)
 gATTAAAGGAAAAACGTGCCGGGTGCGGTTATAAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGATGGCCGCGTCTTATCCGGCCTCCATAAGATTAGCCCGATACGCTTgcg                                                                                                                                                    >  1:1005131/1‑150 (MQ=255)
   ttAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCa                                                                                                                                                  >  1:1153110/1‑150 (MQ=255)
   ttAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCa                                                                                                                                                  >  1:1310101/1‑150 (MQ=255)
        ggAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCggg                                                                                                                                             >  2:1378139/1‑150 (MQ=255)
         gAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAATATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGgc                                                                                                                                            >  2:211125/1‑150 (MQ=255)
         gAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGGGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCTGgc                                                                                                                                            <  2:1330390/150‑1 (MQ=255)
         gAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATGCGCAAGCGTATCGGgc                                                                                                                                            <  2:184128/150‑1 (MQ=255)
           aaaaCGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATGCGCAAGCGTATCGGgcta                                                                                                                                          <  1:611971/150‑3 (MQ=255)
            aaaCGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATGCGCAAGCGTATCGGgctac                                                                                                                                         <  1:405946/150‑4 (MQ=255)
                gTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATcc                                                                                                                                     <  1:1577803/150‑1 (MQ=255)
                 tGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCt                                                                                                                                    >  1:716099/1‑150 (MQ=255)
                  gCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTg                                                                                                                                   >  2:1400962/1‑150 (MQ=255)
                   ccGGGTGCGGTTATTAAAATAATAAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGGCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTgg                                                                                                                                  <  1:1200952/150‑1 (MQ=255)
                    cGGGTGCGGTTATTAAAATAATGAAATGGTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGCGATGGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTggc                                                                                                                                 <  1:1866404/150‑2 (MQ=255)
                      ggTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTAATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTa                                                                                                                               <  1:280396/150‑1 (MQ=255)
                              tattaAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACtt                                                                                                                       <  1:879572/150‑1 (MQ=255)
                              tattaAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACtt                                                                                                                       >  1:720111/1‑150 (MQ=255)
                              tattaAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACtt                                                                                                                       <  1:643128/150‑1 (MQ=255)
                                 taAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCaa                                                                                                                    >  1:1587147/1‑150 (MQ=255)
                                  aaaaTAATGAAATGTTGAATTGCCGGGTGCAAGAGTTAACATCTTATTCGGGATTGCCGGATGCTACGCTGGCATCGTCTTATCAGGCCTCCATAAGAGTAGCCCGAAACGCTTGCGCATCGGGCGCTATCCAGGTTATTTCACTTCAAc                                                                                                                   >  1:118467/1‑150 (MQ=255)
                                  aaaaTAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAAc                                                                                                                   >  2:212538/1‑150 (MQ=255)
                                  aaaaTAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAAc                                                                                                                   <  1:761033/150‑1 (MQ=255)
                                  aaaaTAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGAAACGCTAGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAAc                                                                                                                   >  2:1874599/1‑150 (MQ=255)
                                  aaaaTAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCGTATCCGGCCTCCAGAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGTTTATTTCACGTCacc                                                                                                                   >  2:1267223/1‑148 (MQ=255)
                                    aataatGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACca                                                                                                                 <  2:1946894/150‑1 (MQ=255)
                                        atGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACAtt                                                                                                             >  1:266963/1‑150 (MQ=255)
                                          gAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCa                                                                                                           <  2:1030958/150‑1 (MQ=255)
                                          gAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATAAGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCa                                                                                                           >  1:978600/1‑150 (MQ=255)
                                           aaaTGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAg                                                                                                          <  1:346749/150‑1 (MQ=255)
                                               gtggAATTGCCGGGGGCAAGAGTAAACATCTTATTCGGGACTGCCGCATCCGACGCTGGCCGCGTCTTCTCCGGCCTCCATAAGAGTCGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGGAATTTCACTTCAACCACATTCAGCCGt                                                                                                      <  2:307492/147‑1 (MQ=255)
                                               gTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGt                                                                                                      >  1:458629/1‑150 (MQ=255)
                                                ttGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTa                                                                                                     <  1:747544/150‑1 (MQ=255)
                                                 tGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTAACTTAAAACACATTCAGCCGTaa                                                                                                    >  2:1682097/1‑150 (MQ=255)
                                                  gAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAAc                                                                                                   <  1:1769711/150‑1 (MQ=255)
                                                     ttGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCa                                                                                                <  1:515055/150‑1 (MQ=255)
                                                      tGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGTCGCGGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCAt                                                                                               <  2:519476/150‑1 (MQ=255)
                                                       gCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATc                                                                                              >  2:1279558/1‑150 (MQ=255)
                                                       gCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTACACTTCAACCACATTCCGCCGTAACTCATc                                                                                              >  2:1488123/1‑150 (MQ=255)
                                                               cAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGAt                                                                                      >  2:833587/1‑150 (MQ=255)
                                                                aaGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGACGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCTGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCAACCAACAGAtt                                                                                     >  1:248533/1‑150 (MQ=255)
                                                                 agagTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGAttt                                                                                    >  2:361396/1‑150 (MQ=255)
                                                                         cagcgtATTCGGGATTGCCGGAAGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCAtcttc                                                                            <  2:375796/145‑1 (MQ=255)
                                                                          atgatATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGACTAGCCCGATCCGCGTGCGCATCGGGCGCTATCCTGGTTATATCACTTCAACCACATTCAGCCGGAACTCATCCAACAGATTTTCAtcttct                                                                           <  2:1144638/146‑1 (MQ=255)
                                                                          aTCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCAtcttct                                                                           >  2:1895072/1‑150 (MQ=255)
                                                                           tCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCAtcttctt                                                                          >  2:585949/1‑150 (MQ=255)
                                                                            cTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCAtcttcttc                                                                         <  2:1897821/150‑1 (MQ=255)
                                                                            cTTATTCGGGATTGCCGGATGCGACGCTGGCAGCGTCTTATCCGTCCTCCATAAGAGTAGCCCGATACTCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCTTAACTCATCCAACTGATTTTCAtcttcttc                                                                         <  1:1466947/150‑1 (MQ=255)
                                                                              tATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTg                                                                       <  2:545461/150‑1 (MQ=255)
                                                                               aTTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTgg                                                                      >  1:1594762/1‑150 (MQ=255)
                                                                               aTTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATACGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTAGCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCACCTGATTTTCAACTTCTTCTgg                                                                      >  2:1942298/1‑150 (MQ=255)
                                                                                 tCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGAGACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCt                                                                    <  2:1229980/150‑1 (MQ=255)
                                                                                    ggATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGcc                                                                 <  1:1378139/150‑1 (MQ=255)
                                                                                        ttcaggATGCGACGCTGGCCGCGTCTTATCCGGCCTCCAAAAGAGTAGCCCGATACGCTTGCTCATCTGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCa                                                             <  2:1234468/146‑1 (MQ=255)
                                                                                            ggATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGcc                                                         <  1:1241876/150‑1 (MQ=255)
                                                                                            ggATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGGTATTTGACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGAACGcc                                                         >  2:746231/1‑150 (MQ=255)
                                                                                                 ggACGCTGGCCGCGTCTTATCCGGCCTCAATAAGAGTAGCCCTATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTg                                                    <  1:1406679/149‑1 (MQ=255)
                                                                                                 cGACGCTGGACGCGTCTTATCCGGCCTCAATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGGAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTg                                                    <  1:1399696/150‑1 (MQ=255)
                                                                                                  gACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTAAACCACATTCAGCCGTAACTCATCCAACAGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGgtgt                                                   >  1:1689433/1‑150 (MQ=255)
                                                                                                    cGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCCACTGATCTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAg                                                 >  1:1670230/1‑150 (MQ=255)
                                                                                                    cGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAg                                                 >  2:1128426/1‑150 (MQ=255)
                                                                                                      cTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGGTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTg                                               <  2:243120/150‑1 (MQ=255)
                                                                                                      cTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTg                                               <  1:754389/150‑1 (MQ=255)
                                                                                                      cTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTg                                               >  1:1606995/1‑150 (MQ=255)
                                                                                                       tGGCCGCGTCTTATCCGTCCTCAATAAGAGTAGCCGTATACGTTTGGGCATGGGGGGCTATCCTGGTTATTTCACTTCAACCACTTTCAGCCTTAACTCATCCAACTTATTTTCTTCTTCTTCTGGCTGCCAGCACGCCGGTTGTTGTgg                                              <  2:1005131/150‑1 (MQ=255)
                                                                                                         gCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGa                                            <  1:360542/150‑1 (MQ=255)
                                                                                                         gCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATATTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGa                                            >  1:952182/1‑150 (MQ=255)
                                                                                                         gCCGCGTCGTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGa                                            <  1:1453523/150‑1 (MQ=255)
                                                                                                          ccGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGAt                                           >  1:1880308/1‑150 (MQ=255)
                                                                                                              gTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTCGCTGCCAGCACGCCGGTTGTAGAGGGATctct                                       >  1:663163/1‑150 (MQ=255)
                                                                                                              gTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTGGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTAGGATctct                                       <  2:255823/150‑1 (MQ=255)
                                                                                                                cTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTc                                     >  2:282097/1‑150 (MQ=255)
                                                                                                                cTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCAGCTTCTACTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTc                                     >  1:523273/1‑150 (MQ=255)
                                                                                                                    tCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCCACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGa                                 >  1:1080147/1‑150 (MQ=255)
                                                                                                                     ccGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGAt                                >  1:202604/1‑150 (MQ=255)
                                                                                                                     ccGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGAt                                <  1:398472/150‑1 (MQ=255)
                                                                                                                      cGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATc                               <  1:1147517/150‑1 (MQ=255)
                                                                                                                      cGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATc                               >  2:1078532/1‑150 (MQ=255)
                                                                                                                       ggCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCa                              <  1:206705/150‑1 (MQ=255)
                                                                                                                           tCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTATTCGCGATCAAAcc                          >  2:694167/1‑149 (MQ=255)
                                                                                                                           tCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGGAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGGAGTGGGATCTCTTCGGGATCAAACg                          >  1:829261/1‑150 (MQ=255)
                                                                                                                           tCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTAATTCACTTCAACCACATTCAGCCGTAACCCATCCAACTGATTTTCATCTTCTTCAGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACg                          <  1:1639134/150‑1 (MQ=255)
                                                                                                                            ccATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGAACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGc                         >  1:1533819/1‑150 (MQ=255)
                                                                                                                               tAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAg                      <  2:938359/150‑1 (MQ=255)
                                                                                                                                 agagTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGAt                    >  2:1636184/1‑150 (MQ=255)
                                                                                                                                  cagTCGCCCGCTACGCTTGCGAATCGGGCGCTATCCTGGATATATCACTTCAACCACAAGCAGCCGTAACTCCTCCAACTGATATTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGAAGTGGGATCTCTTCTCGATCAAACTCCAGATc                   <  1:284377/149‑1 (MQ=255)
                                                                                                                                  atagcgCCCGATGCGCAAGCGTATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATc                   >  2:587704/6‑150 (MQ=255)
                                                                                                                                      aGCCCGATACGCTTGCGCATCGGGCGATATCCTGGTTATTACACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTTCCACCACGCCGGTTGTAGTGGGATCTATTCGCGATCAACCGCCAGATCAccc               >  2:67902/1‑150 (MQ=255)
                                                                                                                                       gCCCGATGCGCAAGCGTATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCAcccc              >  1:568620/1‑150 (MQ=255)
                                                                                                                                       gCCCGATGCGCAAGCGTATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCAcccc              >  1:1820511/1‑150 (MQ=255)
                                                                                                                                       gCCCGATGCGCAAGCGTATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCAcccc              >  1:1055744/1‑150 (MQ=255)
                                                                                                                                       gCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCAcccc              >  2:1157539/1‑150 (MQ=255)
                                                                                                                                         ccGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCg            >  1:667295/1‑150 (MQ=255)
                                                                                                                                           gATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGtt          <  1:578665/150‑1 (MQ=255)
                                                                                                                                           gATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGtt          <  1:1182580/150‑1 (MQ=255)
                                                                                                                                             tACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTaa        <  1:542213/150‑1 (MQ=255)
                                                                                                                                                 cTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTACCTCATCCAACTGATTTTCAGCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATATCTTCGCGATCAAACGCCAGATCACCCCCGGTAACCAc    >  2:1269558/1‑150 (MQ=255)
                                                                                                                                                 cTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCAc    >  2:1197056/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
AGATTAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTT  >  NC_000913/1410844‑1411140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: