Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,410,995 C→T 5.6% intergenic (+111/+18) dbpA → / ← ttcA ATP‑dependent RNA helicase, specific for 23S rRNA/tRNA s(2)C32 thioltransferase, iron sulfur cluster protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,410,9950CT 5.6% 447.9 / 9.8 144intergenic (+111/+18)dbpA/ttcAATP‑dependent RNA helicase, specific for 23S rRNA/tRNA s(2)C32 thioltransferase, iron sulfur cluster protein
Reads supporting (aligned to +/- strand):  ref base C (73/62);  new base T (4/4);  total (77/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.03e-01

Only 100 of 148 total aligned reads displayed.

ATTAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAG  >  NC_000913/1410846‑1411143
                                                                                                                                                     |                                                                                                                                                    
cgtcaaGGACACACGTGCCGGGGGCGGTTATTAAAATAATGAAATGGTTAATTGCCGTGTGCAAGATTAAACATCTTTTTAGGGATTGCCGGCTGCGACGCTGGCCGCGTCTTATCCGGCCTCCATTAGCGTAGCCCGATACGCTTgcgc                                                                                                                                                      <  2:1730964/146‑1 (MQ=255)
 ttAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCa                                                                                                                                                     >  1:1153110/1‑150 (MQ=255)
 ttAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCa                                                                                                                                                     >  1:1310101/1‑150 (MQ=255)
      ggAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCggg                                                                                                                                                >  2:9659/1‑150 (MQ=255)
       gAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAATATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGgc                                                                                                                                               >  2:211125/1‑150 (MQ=255)
       gAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGGGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCTGgc                                                                                                                                               <  2:1330390/150‑1 (MQ=255)
       gAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATGCGCAAGCGTATCGGgc                                                                                                                                               <  2:184128/150‑1 (MQ=255)
        aaataCGTTCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCATGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCGTATCCGGCCTCCTTAAGAGGAGCCCGATACGCTTGCGCATCGGgcg                                                                                                                                              <  1:1160814/150‑1 (MQ=255)
         aaaaCGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATGCGCAAGCGTATCGGgcta                                                                                                                                             <  1:611971/150‑3 (MQ=255)
         aaaaCGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGgcgc                                                                                                                                             <  1:1844596/150‑1 (MQ=255)
          aaaCGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATGCGCAAGCGTATCGGgctac                                                                                                                                            <  1:405946/150‑4 (MQ=255)
             cgggacGGGGGAGGTTAGTAAATTAATGAAATGTTGAATTGCCGGGTTCAAGAGTACACATCTTATTCGGGCTTGCCGTATGCGACGCTGGCCGCGTCGAATCCGGCCTCAATACGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATc                                                                                                                                         <  2:1276406/145‑1 (MQ=255)
              gTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATcc                                                                                                                                        <  1:1577803/150‑1 (MQ=255)
               tGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCt                                                                                                                                       >  1:716099/1‑150 (MQ=255)
                gCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTg                                                                                                                                      >  2:1400962/1‑150 (MQ=255)
                 ccGGGTGCGGTTATTAAAATAATAAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGGCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTgg                                                                                                                                     <  1:1200952/150‑1 (MQ=255)
                  cGGGTGCGGTTATTAAAATAATGAAATGGTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGCGATGGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTggc                                                                                                                                    <  1:1866404/150‑2 (MQ=255)
                    ggTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTAATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTa                                                                                                                                  <  1:280396/150‑1 (MQ=255)
                            tattaAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACtt                                                                                                                          <  1:643128/150‑1 (MQ=255)
                            tattaAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACtt                                                                                                                          >  1:720111/1‑150 (MQ=255)
                            tattaAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACtt                                                                                                                          <  1:879572/150‑1 (MQ=255)
                              ttaAAATAATGAAATGTTGAATTGCAGCGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCa                                                                                                                        <  2:1215924/150‑1 (MQ=255)
                               taAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCaa                                                                                                                       >  1:1587147/1‑150 (MQ=255)
                                aaaaTAATGAAATGTTGAATTGCCGGGTGCAAGAGTTAACATCTTATTCGGGATTGCCGGATGCTACGCTGGCATCGTCTTATCAGGCCTCCATAAGAGTAGCCCGAAACGCTTGCGCATCGGGCGCTATCCAGGTTATTTCACTTCAAc                                                                                                                      >  1:118467/1‑150 (MQ=255)
                                aaaaTAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAAc                                                                                                                      <  1:761033/150‑1 (MQ=255)
                                aaaaTAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAAc                                                                                                                      >  2:212538/1‑150 (MQ=255)
                                aaaaTAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGAAACGCTAGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAAc                                                                                                                      >  2:1874599/1‑150 (MQ=255)
                                  aataatGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACca                                                                                                                    <  2:1946894/150‑1 (MQ=255)
                                      atGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACAtt                                                                                                                >  1:266963/1‑150 (MQ=255)
                                         aaaTGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAg                                                                                                             <  1:346749/150‑1 (MQ=255)
                                             gtggAATTGCCGGGGGCAAGAGTAAACATCTTATTCGGGACTGCCGCATCCGACGCTGGCCGCGTCTTCTCCGGCCTCCATAAGAGTCGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGGAATTTCACTTCAACCACATTCAGCCGt                                                                                                         <  2:307492/147‑1 (MQ=255)
                                             gTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGt                                                                                                         >  1:458629/1‑150 (MQ=255)
                                              ttGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTa                                                                                                        <  1:747544/150‑1 (MQ=255)
                                                gAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAAc                                                                                                      <  1:1769711/150‑1 (MQ=255)
                                                   ttGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCa                                                                                                   <  1:515055/150‑1 (MQ=255)
                                                    tGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGTCGCGGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCAt                                                                                                  <  2:519476/150‑1 (MQ=255)
                                                             cAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGAt                                                                                         >  2:833587/1‑150 (MQ=255)
                                                              aaGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGACGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCTGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCAACCAACAGAtt                                                                                        >  1:248533/1‑150 (MQ=255)
                                                               agagTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGAttt                                                                                       >  2:361396/1‑150 (MQ=255)
                                                                  gTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCa                                                                                    >  2:1241876/1‑150 (MQ=255)
                                                                       cagcgtATTCGGGATTGCCGGAAGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCAtcttc                                                                               <  2:375796/145‑1 (MQ=255)
                                                                        atgatATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGACTAGCCCGATCCGCGTGCGCATCGGGCGCTATCCTGGTTATATCACTTCAACCACATTCAGCCGGAACTCATCCAACAGATTTTCAtcttct                                                                              <  2:1144638/146‑1 (MQ=255)
                                                                        aTCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCAtcttct                                                                              >  2:1895072/1‑150 (MQ=255)
                                                                         tCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCAtcttctt                                                                             >  2:585949/1‑150 (MQ=255)
                                                                          cTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCAtcttcttc                                                                            <  2:1897821/150‑1 (MQ=255)
                                                                          cTTATTCGGGATTGCCGGATGCGACGCTGGCAGCGTCTTATCCGTCCTCCATAAGAGTAGCCCGATACTCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCTTAACTCATCCAACTGATTTTCAtcttcttc                                                                            <  1:1466947/150‑1 (MQ=255)
                                                                            tATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTg                                                                          <  2:545461/150‑1 (MQ=255)
                                                                             aTTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTgg                                                                         >  1:1594762/1‑150 (MQ=255)
                                                                             aTTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATACGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTAGCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCACCTGATTTTCAACTTCTTCTgg                                                                         >  2:1942298/1‑150 (MQ=255)
                                                                                  ggATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGcc                                                                    <  1:1378139/150‑1 (MQ=255)
                                                                                   gATTGCCGGATGCGACACTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCa                                                                   >  2:1128282/1‑150 (MQ=255)
                                                                                      ttcaggATGCGACGCTGGCCGCGTCTTATCCGGCCTCCAAAAGAGTAGCCCGATACGCTTGCTCATCTGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCa                                                                <  2:1234468/146‑1 (MQ=255)
                                                                                          ggATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGcc                                                            <  1:1241876/150‑1 (MQ=255)
                                                                                          ggATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGGTATTTGACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGAACGcc                                                            >  2:746231/1‑150 (MQ=255)
                                                                                           gATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCg                                                           >  2:1630660/1‑150 (MQ=255)
                                                                                              gCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGtt                                                        >  2:1461987/1‑150 (MQ=255)
                                                                                               ggACGCTGGCCGCGTCTTATCCGGCCTCAATAAGAGTAGCCCTATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTg                                                       <  1:1406679/149‑1 (MQ=255)
                                                                                               cGACGCTGGACGCGTCTTATCCGGCCTCAATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGGAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTg                                                       <  1:1399696/150‑1 (MQ=255)
                                                                                                gACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTAAACCACATTCAGCCGTAACTCATCCAACAGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGgtgt                                                      >  1:1689433/1‑150 (MQ=255)
                                                                                                  cGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCCACTGATCTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAg                                                    >  1:1670230/1‑150 (MQ=255)
                                                                                                    cTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGGTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTg                                                  <  2:243120/150‑1 (MQ=255)
                                                                                                    cTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTg                                                  <  2:1153110/150‑1 (MQ=255)
                                                                                                    cTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTg                                                  <  1:754389/150‑1 (MQ=255)
                                                                                                    cTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTg                                                  >  1:1606995/1‑150 (MQ=255)
                                                                                                     tGGCCGCGTCTTATCCGTCCTCAATAAGAGTAGCCGTATACGTTTGGGCATGGGGGGCTATCCTGGTTATTTCACTTCAACCACTTTCAGCCTTAACTCATCCAACTTATTTTCTTCTTCTTCTGGCTGCCAGCACGCCGGTTGTTGTgg                                                 <  2:1005131/150‑1 (MQ=255)
                                                                                                       gCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGa                                               <  1:360542/150‑1 (MQ=255)
                                                                                                       gCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATATTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGa                                               >  1:952182/1‑150 (MQ=255)
                                                                                                       gCCGCGTCGTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGa                                               <  1:1453523/150‑1 (MQ=255)
                                                                                                        ccGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGAt                                              >  1:1880308/1‑150 (MQ=255)
                                                                                                            gTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTCGCTGCCAGCACGCCGGTTGTAGAGGGATctct                                          >  1:663163/1‑150 (MQ=255)
                                                                                                            gTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTGGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTAGGATctct                                          <  2:255823/150‑1 (MQ=255)
                                                                                                              cTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCACATGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTc                                        >  2:1351450/1‑150 (MQ=255)
                                                                                                              cTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTc                                        >  2:282097/1‑150 (MQ=255)
                                                                                                              cTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCAGCTTCTACTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTc                                        >  1:523273/1‑150 (MQ=255)
                                                                                                                  tCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCCACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGa                                    >  1:1080147/1‑150 (MQ=255)
                                                                                                                   ccGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGAt                                   <  1:398472/150‑1 (MQ=255)
                                                                                                                   ccGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGAt                                   >  1:202604/1‑150 (MQ=255)
                                                                                                                   ccGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGAt                                   <  1:1133067/150‑1 (MQ=255)
                                                                                                                    cGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATc                                  >  2:1078532/1‑150 (MQ=255)
                                                                                                                     ggCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCa                                 <  1:206705/150‑1 (MQ=255)
                                                                                                                         tCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTATTCGCGATCAAAcc                             >  2:694167/1‑149 (MQ=255)
                                                                                                                         tCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGGAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGGAGTGGGATCTCTTCGGGATCAAACg                             >  1:829261/1‑150 (MQ=255)
                                                                                                                         tCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTAATTCACTTCAACCACATTCAGCCGTAACCCATCCAACTGATTTTCATCTTCTTCAGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACg                             <  1:1639134/150‑1 (MQ=255)
                                                                                                                          ccATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGAACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGc                            >  1:1533819/1‑150 (MQ=255)
                                                                                                                             tAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAg                         <  2:938359/150‑1 (MQ=255)
                                                                                                                               agagTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGAt                       >  2:1636184/1‑150 (MQ=255)
                                                                                                                                cagTCGCCCGCTACGCTTGCGAATCGGGCGCTATCCTGGATATATCACTTCAACCACAAGCAGCCGTAACTCCTCCAACTGATATTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGAAGTGGGATCTCTTCTCGATCAAACTCCAGATc                      <  1:284377/149‑1 (MQ=255)
                                                                                                                                atagcgCCCGATGCGCAAGCGTATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATc                      >  2:587704/6‑150 (MQ=255)
                                                                                                                                 agTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCa                     >  2:1026785/1‑150 (MQ=255)
                                                                                                                                    aGCCCGATACGCTTGCGCATCGGGCGATATCCTGGTTATTACACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTTCCACCACGCCGGTTGTAGTGGGATCTATTCGCGATCAACCGCCAGATCAccc                  >  2:67902/1‑150 (MQ=255)
                                                                                                                                     gCCCGATGCGCAAGCGTATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCAcccc                 >  1:1055744/1‑150 (MQ=255)
                                                                                                                                     gCCCGATGCGCAAGCGTATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCAcccc                 >  1:1820511/1‑150 (MQ=255)
                                                                                                                                     gCCCGATGCGCAAGCGTATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCAcccc                 >  1:568620/1‑150 (MQ=255)
                                                                                                                                       ccGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCg               >  1:667295/1‑150 (MQ=255)
                                                                                                                                         gATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGtt             <  1:578665/150‑1 (MQ=255)
                                                                                                                                           tACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTaa           <  1:542213/150‑1 (MQ=255)
                                                                                                                                             cGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAAcc         <  1:977054/150‑1 (MQ=255)
                                                                                                                                               cTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTACCTCATCCAACTGATTTTCAGCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATATCTTCGCGATCAAACGCCAGATCACCCCCGGTAACCAc       >  2:1269558/1‑150 (MQ=255)
                                                                                                                                               cTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCAc       >  2:1197056/1‑150 (MQ=255)
                                                                                                                                                  gcgcATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGCCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTc    <  1:118389/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ATTAAAGGAAAAACGTGCCGGGTGCGGTTATTAAAATAATGAAATGTTGAATTGCCGGGTGCAAGAGTAAACATCTTATTCGGGATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTCCATAAGAGTAGCCCGATACGCTTGCGCATCGGGCGCTATCCTGGTTATTTCACTTCAACCACATTCAGCCGTAACTCATCCAACTGATTTTCATCTTCTTCTGGCTGCCAGCACGCCGGTTGTAGTGGGATCTCTTCGCGATCAAACGCCAGATCACCCCCGTTAACCACTTCAG  >  NC_000913/1410846‑1411143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: