Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,449,802 A→G 5.9% A516A (GCT→GCC) ‡ tynA ← tyramine oxidase, copper‑requiring

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,449,8020AG 5.9% 351.6 / 9.3 119A516A (GCT→GCC) ‡tynAtyramine oxidase, copper‑requiring
Reads supporting (aligned to +/- strand):  ref base A (55/56);  new base G (3/4);  total (59/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.24e-01

Only 100 of 121 total aligned reads displayed.

ACATCCAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTTATACTC  >  NC_000913/1449653‑1449951
                                                                                                                                                     |                                                                                                                                                     
aCATCCAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTa                                                                                                                                                       >  2:1714368/1‑150 (MQ=255)
  aTCCAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACCCCTTTCACCGCTTTGATGCCCGTAGc                                                                                                                                                     >  2:1804449/1‑150 (MQ=255)
  aTCCAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGc                                                                                                                                                     >  2:1000937/1‑150 (MQ=255)
  aTCCAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGc                                                                                                                                                     <  1:31248/150‑1 (MQ=255)
   tCCAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGATTTAACACCTTTCACCGCTTCGATGCCCGTAGCa                                                                                                                                                    >  1:1254197/1‑150 (MQ=255)
    ccAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCAc                                                                                                                                                   >  1:1092444/1‑150 (MQ=255)
     cAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCAcc                                                                                                                                                  >  2:1676704/1‑150 (MQ=255)
      aGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCg                                                                                                                                                 <  2:1119243/150‑1 (MQ=255)
         tagtggCGGAAATTATATATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGGCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGTCa                                                                                                                                              <  2:1250295/146‑1 (MQ=255)
         tCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCa                                                                                                                                              <  1:1398846/150‑1 (MQ=255)
           gAGGCTGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCGGTGCTACGGGCATc                                                                                                                                            <  2:1515795/150‑1 (MQ=255)
           gAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATc                                                                                                                                            <  1:1725879/150‑1 (MQ=255)
            aGGCGGAAATTATAAATCTGTTGGTGTGTAGTACCCACGATATTGTGATCGATAATCTTGACGTAGCTCGTGTCATCTTTCGCCGTCTCATAGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCg                                                                                                                                           <  2:314254/150‑1 (MQ=255)
            aGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCGGTGCTACGGGCATCg                                                                                                                                           <  1:867911/150‑1 (MQ=255)
              gCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGAt                                                                                                                                         >  2:258420/1‑150 (MQ=255)
                ggAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGc                                                                                                                                       >  1:256472/1‑150 (MQ=255)
                ggAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGAGCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCATCGATGCCGGTGCTACGGGCATCGAAGc                                                                                                                                       <  1:1313436/150‑1 (MQ=255)
                    aTTATAAATATGTTGGGGTGTAGTACCCACGATATTGTGATCGATAAGCGGGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAAt                                                                                                                                   <  1:618180/150‑1 (MQ=255)
                     ttATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATa                                                                                                                                  >  2:642442/1‑150 (MQ=255)
                      tataAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCTGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAg                                                                                                                                 >  2:1825915/1‑150 (MQ=255)
                      tataAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAg                                                                                                                                 <  1:1194893/150‑1 (MQ=255)
                      tataAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTGTCGCTTTAACACCTTTCACCGATTCGATGCCCGTAGCACCGGCATCGATGCCAATAg                                                                                                                                 >  1:347734/1‑150 (MQ=255)
                        taAATTTGGTGGTGTGTAGTACCCACGATATTGTGTTCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTGATCGTGCATGGTTTTCGGTTTAACACCTTGCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTg                                                                                                                               <  2:669405/150‑1 (MQ=255)
                         aaaTATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCCCCGCTTCGATGCCCGTCGCACCTGCGGCGATGCCAATAGGGc                                                                                                                              >  2:949835/1‑150 (MQ=255)
                         aaaTATGTTGGTGGGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGAACCGGCATCGATGCCAATAGTGc                                                                                                                              <  1:139673/150‑1 (MQ=255)
                          aaTATGTTGGTGTGTAGTCCCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCATTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGcc                                                                                                                             <  1:1089533/150‑1 (MQ=255)
                          aaTATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGcc                                                                                                                             <  2:1761964/150‑1 (MQ=255)
                          aaTATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTGTCGCTTTAACACCTTTCCCCGCTTCGATGCCAGTAGCACCGGCATCGATGCCAATAGTGcc                                                                                                                             >  1:340091/1‑150 (MQ=255)
                                   gtgtgtAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATg                                                                                                                    <  1:419704/150‑1 (MQ=255)
                                   gtgtgtAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATg                                                                                                                    <  1:1900569/150‑1 (MQ=255)
                                   gggtgtAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCCTCTCAGCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATg                                                                                                                    <  2:342960/148‑1 (MQ=255)
                                    tgtgtAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATgg                                                                                                                   <  2:1903657/150‑1 (MQ=255)
                                    ggtgtAGTACCCACGATATTGTGATCGATAAGCGGGCCGTAGCGCGGGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATgg                                                                                                                   <  2:1387843/149‑1 (MQ=255)
                                    agtgtAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATgg                                                                                                                   >  2:13553/2‑150 (MQ=255)
                                          gtaCCCACGATATTGTGATCGATAAGCGGGCCGTAGCGCGTGGCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATc                                                                                                             <  2:1245346/150‑1 (MQ=255)
                                           taCACACGATATTGTGATCGATAAGCTTGCCGAAGCGCGGGTAATCTATCGCCGTCTCATCGTGCATGGGTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGTCATCGATGCCAAAAGTGCCGTTTTCATGGAAGATcc                                                                                                            <  1:1573966/150‑1 (MQ=255)
                                            aCCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCa                                                                                                           <  1:1197072/150‑1 (MQ=255)
                                             cccACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAg                                                                                                          >  2:1558186/1‑150 (MQ=255)
                                              ccACGATATTGTGATCGATAAGCGTGCCGCAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTCTCACCGCTTCGATGCCCGTAGCCCCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGt                                                                                                         >  2:1911826/1‑150 (MQ=255)
                                                       tgtgATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGt                                                                                                <  2:256472/150‑1 (MQ=255)
                                                       tgtgATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGt                                                                                                >  2:191259/1‑150 (MQ=255)
                                                        gtgATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTa                                                                                               >  2:91258/1‑150 (MQ=255)
                                                           aTCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGACCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTc                                                                                            <  2:1771022/150‑1 (MQ=255)
                                                              gATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATa                                                                                         <  1:258420/150‑1 (MQ=255)
                                                                  aGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTa                                                                                     >  1:289609/1‑150 (MQ=255)
                                                                  aGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATACAGTCAAAAATGTAGTCATAGTTa                                                                                     >  1:341533/1‑150 (MQ=255)
                                                                  aGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGACGTTTTCATGGAAGATCAAGTCAAAAATGTAGTCATAGTTa                                                                                     >  2:1225200/1‑150 (MQ=255)
                                                                   gCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGATGATCCAGTCAAAAATGTAGTCATAGTTAc                                                                                    >  1:1019887/1‑150 (MQ=255)
                                                                   gCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGtttc                                                                                    >  1:1853535/1‑148 (MQ=255)
                                                                     gTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTAccc                                                                                  <  2:1092444/150‑1 (MQ=255)
                                                                       gCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCAc                                                                                <  1:1295652/150‑1 (MQ=255)
                                                                         cGTAGCGCGTGTCATCTTTAGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTg                                                                              <  1:1770284/150‑1 (MQ=255)
                                                                            aGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTAc                                                                           <  2:966186/150‑1 (MQ=255)
                                                                            aGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTAc                                                                           <  1:3482/150‑1 (MQ=255)
                                                                              cgcgTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTCCCCACAGTACTg                                                                         >  2:145545/1‑150 (MQ=255)
                                                                              cgcgTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTg                                                                         >  1:1968175/1‑150 (MQ=255)
                                                                                  tgtCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATcc                                                                     <  2:241622/150‑1 (MQ=255)
                                                                                  tgtCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATcc                                                                     >  1:887520/1‑150 (MQ=255)
                                                                                    tcttCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAg                                                                   <  2:1993173/147‑1 (MQ=255)
                                                                                       tCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAgcgc                                                                <  1:1410471/150‑1 (MQ=255)
                                                                                       acTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGATTCGATGCCCGTAGCACCGGCATAGATGCCAATAGTGCCGTTTTCATGGAAGATACAGTCAAAAATGTAGTCATAGTTACCGACTGTACTGATCCAgcgc                                                                >  1:71158/2‑150 (MQ=255)
                                                                                          ttCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTTCTGATCCAGCGcacc                                                             >  1:1279116/1‑150 (MQ=255)
                                                                                          ttCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGcacc                                                             >  1:797947/1‑150 (MQ=255)
                                                                                          ttCGCCGTCTCATCGAGCATGGTATTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTATCACCGGGATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGcacc                                                             <  2:315632/150‑1 (MQ=255)
                                                                                              ccGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGGTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTa                                                         <  2:334089/150‑1 (MQ=255)
                                                                                                gTCTCAGCGTGCATTGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCAACGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAAc                                                       <  1:1015768/150‑1 (MQ=255)
                                                                                                    cATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGCTGCCCGTAGCACCGGCATCGCTGCCAATAGTGCCGTTTTCATGGAAGCTCCAGGCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTccc                                                   >  1:1228614/1‑150 (MQ=255)
                                                                                                     aTCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCg                                                  <  1:1585168/150‑1 (MQ=255)
                                                                                                        gTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCggag                                               >  1:585048/1‑148 (MQ=255)
                                                                                                          gCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGtt                                             >  1:605358/1‑150 (MQ=255)
                                                                                                            aTGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCg                                           <  1:1027872/150‑1 (MQ=255)
                                                                                                             tGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCgg                                          <  1:1714368/150‑1 (MQ=255)
                                                                                                             tGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCgg                                          <  1:608697/150‑1 (MQ=255)
                                                                                                                ttttCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTAc                                       <  1:91258/150‑1 (MQ=255)
                                                                                                                ttttCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTAc                                       >  1:869846/1‑150 (MQ=255)
                                                                                                                    cGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATACAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGTTACTGAc                                   >  1:569428/1‑150 (MQ=255)
                                                                                                                     gCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTACCTCCCGGCGTTCGTTACTGAcc                                  >  2:220753/1‑149 (MQ=255)
                                                                                                                        ttAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTg                               >  1:1035144/1‑150 (MQ=255)
                                                                                                                           acacCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGGTCGGTACTGACGTTGGGc                            >  1:874282/1‑150 (MQ=255)
                                                                                                                              ccTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTgg                         >  2:69508/1‑150 (MQ=255)
                                                                                                                                tttCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGcc                       <  2:797947/150‑1 (MQ=255)
                                                                                                                                tttCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGcc                       <  2:805162/150‑1 (MQ=255)
                                                                                                                                 ttCACCACTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTAGTGACGTTGGGCTGGccc                      >  2:1170259/1‑150 (MQ=255)
                                                                                                                                    aCCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCCCCCCTAACTCCCGGCGTGCGGTCCTGACGTTGGGCTGGCCCAtt                   >  2:1704366/1‑150 (MQ=255)
                                                                                                                                      cGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAAATCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTc                 >  2:1525769/1‑150 (MQ=255)
                                                                                                                                       gCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTcc                <  1:1474531/150‑1 (MQ=255)
                                                                                                                                        catCGATGCCGGTGCTACGGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCt               >  2:1624597/3‑150 (MQ=255)
                                                                                                                                        cTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCt               <  2:964447/150‑1 (MQ=255)
                                                                                                                                        cTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCt               <  2:1943048/150‑1 (MQ=255)
                                                                                                                                        cTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCt               <  2:1742364/150‑1 (MQ=255)
                                                                                                                                         atCGATGCCGGTGCTACGGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTGCTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTTCTGACGTTGGGCTGGCCCATTTCCTg              >  1:1300324/2‑150 (MQ=255)
                                                                                                                                           cGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGAt            <  2:289609/150‑1 (MQ=255)
                                                                                                                                           cGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTATTCATGGAAGATCCAGTCAAAAATGTACTCATAGTTAACCACAGTACTGATCAAGCGCACCACTAACTCCCTGCGTTCGGAACTAAAGATGGACTGTACCATTTACTGAt            >  2:1593830/1‑150 (MQ=255)
                                                                                                                                           cGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGGTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGCCGTTGGGCTGGCCCATGTCCTGAt            >  1:598983/1‑150 (MQ=255)
                                                                                                                                              tGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCt         <  1:1558186/150‑1 (MQ=255)
                                                                                                                                              tGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCt         <  2:1039852/150‑1 (MQ=255)
                                                                                                                                                cccGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTta       >  1:1634272/1‑150 (MQ=255)
                                                                                                                                                cccGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTta       >  1:1653636/1‑150 (MQ=255)
                                                                                                                                                    tAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTTATACt   <  1:1000937/150‑1 (MQ=255)
                                                                                                                                                     aGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTTATACTc  >  1:224094/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACATCCAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTTATACTC  >  NC_000913/1449653‑1449951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: