Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,449,803 G→C 5.8% A516G (GCT→GGT) ‡ tynA ← tyramine oxidase, copper‑requiring

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,449,8030GC 5.8% 379.8 / 12.9 120A516G (GCT→GGT) ‡tynAtyramine oxidase, copper‑requiring
Reads supporting (aligned to +/- strand):  ref base G (57/55);  new base C (3/4);  total (60/60)
Fisher's exact test for biased strand distribution p-value = 7.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-01

Only 100 of 120 total aligned reads displayed.

ATCCAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTTATACTC  >  NC_000913/1449655‑1449951
                                                                                                                                                    |                                                                                                                                                    
aTCCAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGc                                                                                                                                                     >  2:1000937/1‑150 (MQ=255)
 tCCAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGATTTAACACCTTTCACCGCTTCGATGCCCGTAGCa                                                                                                                                                    >  1:1254197/1‑150 (MQ=255)
  ccAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCAc                                                                                                                                                   >  1:1092444/1‑150 (MQ=255)
    aGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCg                                                                                                                                                 <  2:1119243/150‑1 (MQ=255)
       tagtggCGGAAATTATATATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGGCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGTCa                                                                                                                                              <  2:1250295/146‑1 (MQ=255)
       tCGTGGCTGATAGTATTAATTTGTTGGTGTGTAGTACAGAAGATATTGTGTTCGTTTAGAGTTCGGTAGCGCGTGTCATATTTCGCCGTCTCATCGTGCATGGTTTTCGCTGTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCa                                                                                                                                              <  1:349262/150‑1 (MQ=255)
       tCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCa                                                                                                                                              <  1:1398846/150‑1 (MQ=255)
         gAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATc                                                                                                                                            <  1:1725879/150‑1 (MQ=255)
          aGGCGGAAATTATAAATCTGTTGGTGTGTAGTACCCACGATATTGTGATCGATAATCTTGACGTAGCTCGTGTCATCTTTCGCCGTCTCATAGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCg                                                                                                                                           <  2:314254/150‑1 (MQ=255)
          aGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCGGTGCTACGGGCATCg                                                                                                                                           <  1:867911/150‑1 (MQ=255)
             cGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCATCGCTTCGATGCCCGTAGCACCGGCATCGATg                                                                                                                                        >  2:1381956/1‑150 (MQ=255)
             cGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCGGTGCTACGGGCATCGaag                                                                                                                                        <  2:1560011/150‑3 (MQ=255)
              ggAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGc                                                                                                                                       >  1:256472/1‑150 (MQ=255)
              ggAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGAGCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCATCGATGCCGGTGCTACGGGCATCGAAGc                                                                                                                                       <  1:1313436/150‑1 (MQ=255)
                  aTTATAAATATGTTGGGGTGTAGTACCCACGATATTGTGATCGATAAGCGGGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAAt                                                                                                                                   <  1:618180/150‑1 (MQ=255)
                   ttATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATa                                                                                                                                  >  2:642442/1‑150 (MQ=255)
                    tataAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCTGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAg                                                                                                                                 >  2:1825915/1‑150 (MQ=255)
                    tataAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAg                                                                                                                                 <  1:1194893/150‑1 (MQ=255)
                    tataAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTGTCGCTTTAACACCTTTCACCGATTCGATGCCCGTAGCACCGGCATCGATGCCAATAg                                                                                                                                 >  1:347734/1‑150 (MQ=255)
                      taAATTTGGTGGTGTGTAGTACCCACGATATTGTGTTCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTGATCGTGCATGGTTTTCGGTTTAACACCTTGCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTg                                                                                                                               <  2:669405/150‑1 (MQ=255)
                       aaaTATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCCCCGCTTCGATGCCCGTCGCACCTGCGGCGATGCCAATAGGGc                                                                                                                              >  2:949835/1‑150 (MQ=255)
                       aaaTATGTTGGTGGGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGAACCGGCATCGATGCCAATAGTGc                                                                                                                              <  1:139673/150‑1 (MQ=255)
                        aaTATGTTGGTGTGTAGTCCCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCATTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGcc                                                                                                                             <  1:1089533/150‑1 (MQ=255)
                        aaTATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGcc                                                                                                                             <  2:1761964/150‑1 (MQ=255)
                        aaTATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTGTCGCTTTAACACCTTTCCCCGCTTCGATGCCAGTAGCACCGGCATCGATGCCAATAGTGcc                                                                                                                             >  1:340091/1‑150 (MQ=255)
                                 gtgtgtAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAAGACCTTTCACCGCTTCGTTGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATg                                                                                                                    <  2:28696/150‑1 (MQ=255)
                                 gtgtgtAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATg                                                                                                                    <  1:1900569/150‑1 (MQ=255)
                                  tgtgtAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATgg                                                                                                                   <  2:1903657/150‑1 (MQ=255)
                                  ggtgtAGTACCCACGATATTGTGATCGATAAGCGGGCCGTAGCGCGGGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATgg                                                                                                                   <  2:1387843/149‑1 (MQ=255)
                                  agtgtAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATgg                                                                                                                   >  2:13553/2‑150 (MQ=255)
                                         taCACACGATATTGTGATCGATAAGCTTGCCGAAGCGCGGGTAATCTATCGCCGTCTCATCGTGCATGGGTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGTCATCGATGCCAAAAGTGCCGTTTTCATGGAAGATcc                                                                                                            <  1:1573966/150‑1 (MQ=255)
                                          aCCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCa                                                                                                           <  1:1197072/150‑1 (MQ=255)
                                           cccACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAg                                                                                                          >  2:1558186/1‑150 (MQ=255)
                                            ccACGATATTGTGATCGATAAGCGTGCCGCAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTCTCACCGCTTCGATGCCCGTAGCCCCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGt                                                                                                         >  2:1911826/1‑150 (MQ=255)
                                                     tgtgATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGt                                                                                                <  2:256472/150‑1 (MQ=255)
                                                     tgtgATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGt                                                                                                >  2:191259/1‑150 (MQ=255)
                                                      gtgATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTa                                                                                               >  2:91258/1‑150 (MQ=255)
                                                         aTCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGACCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTc                                                                                            <  2:1771022/150‑1 (MQ=255)
                                                            gATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATa                                                                                         <  1:258420/150‑1 (MQ=255)
                                                                aGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTa                                                                                     >  1:289609/1‑150 (MQ=255)
                                                                aGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATACAGTCAAAAATGTAGTCATAGTTa                                                                                     >  1:341533/1‑150 (MQ=255)
                                                                aGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGACGTTTTCATGGAAGATCAAGTCAAAAATGTAGTCATAGTTa                                                                                     >  2:1225200/1‑150 (MQ=255)
                                                                 gCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGATGATCCAGTCAAAAATGTAGTCATAGTTAc                                                                                    >  1:1019887/1‑150 (MQ=255)
                                                                 gCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGtttc                                                                                    >  1:1853535/1‑148 (MQ=255)
                                                                   gTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTAccc                                                                                  <  2:1092444/150‑1 (MQ=255)
                                                                     gCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCAc                                                                                <  1:1295652/150‑1 (MQ=255)
                                                                       cGTAGCGCGTGTCATCTTTAGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTg                                                                              <  1:1770284/150‑1 (MQ=255)
                                                                          aGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTAc                                                                           <  2:966186/150‑1 (MQ=255)
                                                                          aGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTAc                                                                           <  1:3482/150‑1 (MQ=255)
                                                                            cgcgTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTCCCCACAGTACTg                                                                         >  2:145545/1‑150 (MQ=255)
                                                                                tgtCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATcc                                                                     <  2:241622/150‑1 (MQ=255)
                                                                                tgtCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATcc                                                                     >  1:887520/1‑150 (MQ=255)
                                                                                tgtCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATcc                                                                     <  1:599406/150‑1 (MQ=255)
                                                                                  tcttCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAg                                                                   <  2:1993173/147‑1 (MQ=255)
                                                                                  tCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAg                                                                   >  2:501440/1‑150 (MQ=255)
                                                                                   cATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAgc                                                                  >  2:1830633/1‑150 (MQ=255)
                                                                                     tCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAgcgc                                                                <  1:1410471/150‑1 (MQ=255)
                                                                                     acTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGATTCGATGCCCGTAGCACCGGCATAGATGCCAATAGTGCCGTTTTCATGGAAGATACAGTCAAAAATGTAGTCATAGTTACCGACTGTACTGATCCAgcgc                                                                >  1:71158/2‑150 (MQ=255)
                                                                                        ttCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTTCTGATCCAGCGcacc                                                             >  1:1279116/1‑150 (MQ=255)
                                                                                        ttCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGcacc                                                             >  1:805162/1‑150 (MQ=255)
                                                                                        ttCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGcacc                                                             >  1:797947/1‑150 (MQ=255)
                                                                                        ttCGCCGTCTCATCGAGCATGGTATTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTATCACCGGGATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGcacc                                                             <  2:315632/150‑1 (MQ=255)
                                                                                            ccGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGGTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTa                                                         <  2:334089/150‑1 (MQ=255)
                                                                                              gTCTCAGCGTGCATTGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCAACGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAAc                                                       <  1:1015768/150‑1 (MQ=255)
                                                                                                  cATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGCTGCCCGTAGCACCGGCATCGCTGCCAATAGTGCCGTTTTCATGGAAGCTCCAGGCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTccc                                                   >  1:1228614/1‑150 (MQ=255)
                                                                                                  cATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTccc                                                   <  2:1197928/150‑1 (MQ=255)
                                                                                                   aTCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCg                                                  <  1:1585168/150‑1 (MQ=255)
                                                                                                      gTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCggag                                               >  1:585048/1‑148 (MQ=255)
                                                                                                        gCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGtt                                             >  1:605358/1‑150 (MQ=255)
                                                                                                          aTGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCg                                           <  1:1027872/150‑1 (MQ=255)
                                                                                                              ttttCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTAc                                       <  1:91258/150‑1 (MQ=255)
                                                                                                                 tCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGa                                    <  1:1676704/150‑1 (MQ=255)
                                                                                                                  cGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTGCGGTACTGAc                                   >  2:1981489/1‑150 (MQ=255)
                                                                                                                  cGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATACAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGTTACTGAc                                   >  1:569428/1‑150 (MQ=255)
                                                                                                                   gCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTACCTCCCGGCGTTCGTTACTGAcc                                  >  2:220753/1‑149 (MQ=255)
                                                                                                                   gCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACg                                  >  2:867993/1‑150 (MQ=255)
                                                                                                                      ttAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTg                               >  1:1035144/1‑150 (MQ=255)
                                                                                                                      ttAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTg                               >  1:1911348/1‑150 (MQ=255)
                                                                                                                         acacCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGGTCGGTACTGACGTTGGGc                            >  1:874282/1‑150 (MQ=255)
                                                                                                                            ccTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTgg                         >  2:69508/1‑150 (MQ=255)
                                                                                                                              tttCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGcc                       <  2:797947/150‑1 (MQ=255)
                                                                                                                               ttCACCACTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTAGTGACGTTGGGCTGGccc                      >  2:1170259/1‑150 (MQ=255)
                                                                                                                                 cACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCAt                    >  1:915745/1‑150 (MQ=255)
                                                                                                                                  aCCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCCCCCCTAACTCCCGGCGTGCGGTCCTGACGTTGGGCTGGCCCAtt                   >  2:1704366/1‑150 (MQ=255)
                                                                                                                                    cGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAAATCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTc                 >  2:1525769/1‑150 (MQ=255)
                                                                                                                                     gCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTcc                <  1:1474531/150‑1 (MQ=255)
                                                                                                                                      catCGATGCCGGTGCTACGGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCt               >  2:1624597/3‑150 (MQ=255)
                                                                                                                                      cTTCGATGCCCGTAGCACCGTCATCGATGCCAATAGTGCCGTTTTAATGGAAGATCCAGTCAAATATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCAGCACTAACTCCCGGCGTTCGGTACTGACGTTTGGCTGGCCCATTTTCt               <  1:301297/150‑1 (MQ=255)
                                                                                                                                      cTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCt               <  2:1742364/150‑1 (MQ=255)
                                                                                                                                      cTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCt               <  2:964447/150‑1 (MQ=255)
                                                                                                                                       atCGATGCCGGTGCTACGGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTGCTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTTCTGACGTTGGGCTGGCCCATTTCCTg              >  1:1300324/2‑150 (MQ=255)
                                                                                                                                         cGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGAt            <  2:289609/150‑1 (MQ=255)
                                                                                                                                         cGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTATTCATGGAAGATCCAGTCAAAAATGTACTCATAGTTAACCACAGTACTGATCAAGCGCACCACTAACTCCCTGCGTTCGGAACTAAAGATGGACTGTACCATTTACTGAt            >  2:1593830/1‑150 (MQ=255)
                                                                                                                                         cGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGGTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGCCGTTGGGCTGGCCCATGTCCTGAt            >  1:598983/1‑150 (MQ=255)
                                                                                                                                          gATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATg           >  2:1727932/1‑150 (MQ=255)
                                                                                                                                            tGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCt         <  1:1558186/150‑1 (MQ=255)
                                                                                                                                              cccGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTta       >  1:1653636/1‑150 (MQ=255)
                                                                                                                                              cccGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTta       >  1:1634272/1‑150 (MQ=255)
                                                                                                                                                  tAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTTATACt   <  1:1000937/150‑1 (MQ=255)
                                                                                                                                                   aGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTTATACTc  >  1:224094/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATCCAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTTATACTC  >  NC_000913/1449655‑1449951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: