Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,457,983 A→T 6.3% I163F (ATC→TTC)  paaF → 2,3‑dehydroadipyl‑CoA hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,457,9830AT 6.3% 322.5 / 9.2 111I163F (ATC→TTC) paaF2,3‑dehydroadipyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  ref base A (46/58);  new base T (3/4);  total (49/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.36e-01

Only 100 of 116 total aligned reads displayed.

TGTGCGATGTGGTGGTTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCG  >  NC_000913/1457834‑1458132
                                                                                                                                                     |                                                                                                                                                     
tgtgCGATGTGGTGGTTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTa                                                                                                                                                       >  1:1740124/1‑150 (MQ=255)
tgtgCGATGTGGTGGTTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTa                                                                                                                                                       >  1:173671/1‑150 (MQ=255)
         tggtggTTGCCGGATAGAAAGAGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTc                                                                                                                                              >  2:648688/1‑150 (MQ=255)
         tggtggTTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTc                                                                                                                                              >  1:1866953/1‑150 (MQ=255)
         tggtggTTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTc                                                                                                                                              <  1:654890/150‑1 (MQ=255)
          ggtggtTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGTACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAATGTATCACCGCTCa                                                                                                                                             >  1:851378/1‑150 (MQ=255)
          ggtggtTGCCGGAGAGAACGCGCGTTTTGGGTGGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGTGATACTTTCTCCGCTCa                                                                                                                                             <  2:716902/150‑1 (MQ=255)
           gtggtTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCag                                                                                                                                            <  1:1990837/150‑1 (MQ=255)
            tggtTGCCGGAGAGAACGCGCTTTTTGGGGTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCTTTAGCCATCAAAATGGTGCTGATAGGAGAAAGTATCACCGCTCagc                                                                                                                                           <  2:1107819/150‑1 (MQ=255)
            tggtTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGTGATACTTTCTCCGCTCagc                                                                                                                                           <  1:1961062/150‑1 (MQ=255)
            tggtTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCagc                                                                                                                                           <  1:1631677/150‑1 (MQ=255)
            gggtTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGGGCTGAGCGGAGAAAGTATCACCGCTCagc                                                                                                                                           <  2:1116104/149‑1 (MQ=255)
              gtTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGTGATACTTTCTCCGCTCagcac                                                                                                                                         <  1:699028/150‑2 (MQ=255)
                  ccGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGcac                                                                                                                                     <  1:1905249/150‑1 (MQ=255)
                    ggAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCAcag                                                                                                                                   >  2:162051/1‑150 (MQ=255)
                    ggAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCAcag                                                                                                                                   >  1:1836208/1‑150 (MQ=255)
                     gagagaACGCGCGTTTTGGGTGGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTAAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCAcagc                                                                                                                                  <  1:465069/150‑1 (MQ=255)
                      agagaACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGAGCAGGCGGAACGCAACGGTTAATCCGTAGGGTCGGGAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGCGAAAGTATCACAGCTCAGCACGCAcagca                                                                                                                                 >  2:164157/1‑150 (MQ=255)
                        agaACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAgg                                                                                                                               <  2:1080676/150‑1 (MQ=255)
                           aCGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCg                                                                                                                            <  2:1772842/150‑1 (MQ=255)
                           aCGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCg                                                                                                                            >  1:1641784/1‑150 (MQ=255)
                            cgcgcgTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCgg                                                                                                                           >  1:677168/1‑150 (MQ=255)
                            cgcgcgTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCgg                                                                                                                           >  1:32176/1‑150 (MQ=255)
                                 gtgttGGGTTGCCGGAAATCACTCTCGGCATGATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGGCGGTAAAGCGGTAGCCAGCAAAATGGTGCGGAGCGGAGAAAGTATCTCCGCTCAGCAAGCACAGCAGGCcgggcggg                                                                                                                      <  1:1999307/147‑1 (MQ=255)
                                 gTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTgg                                                                                                                      <  1:1553538/150‑1 (MQ=255)
                                  ttttGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGt                                                                                                                     <  1:1031392/150‑1 (MQ=255)
                                     tGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTCGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAg                                                                                                                  <  2:1115865/150‑1 (MQ=255)
                                       ggTTGCCGGAACTCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCg                                                                                                                <  2:1647776/150‑1 (MQ=255)
                                        gTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGa                                                                                                               >  1:71635/1‑150 (MQ=255)
                                        gTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGa                                                                                                               <  2:1093332/150‑1 (MQ=255)
                                          tGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACg                                                                                                             <  1:1995259/150‑1 (MQ=255)
                                          ggCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACg                                                                                                             <  1:1671851/149‑1 (MQ=255)
                                                  aTCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGGAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTcccc                                                                                                     <  2:1063998/150‑1 (MQ=255)
                                                    cACTCTCGGGATCAGGCCTGCCGCCGGCGGAACGCAACGATTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCATTTTGTTGATTAGCGGAGAATTTATCACCGCTCTTCAAGTAAATCATGTCTTTCTGGTTAGGTAGGTCTTCCCCAg                                                                                                   <  2:335618/150‑1 (MQ=255)
                                                    cACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGCCGTCTTCCCCAg                                                                                                   >  2:1697667/1‑150 (MQ=255)
                                                    cACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAACTATCACCGCTCAGCAAGCACAGCAGTCCGGCCTGGTTAGCGACGTCTTCCCAAg                                                                                                   >  2:529482/1‑150 (MQ=255)
                                                     aCTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGc                                                                                                  >  1:1126597/1‑150 (MQ=255)
                                                      ctctCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCg                                                                                                 >  2:155817/1‑150 (MQ=255)
                                                      ctctCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCg                                                                                                 >  2:1356171/1‑150 (MQ=255)
                                                      ctctCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCg                                                                                                 >  2:1923365/1‑150 (MQ=255)
                                                          cGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGAttt                                                                                             >  1:1607654/1‑150 (MQ=255)
                                                          cGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGGGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCACGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCCGCGAttt                                                                                             >  1:1599278/1‑150 (MQ=255)
                                                           ggCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTa                                                                                            <  2:435919/150‑1 (MQ=255)
                                                             catcatGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAAc                                                                                          >  2:1014975/1‑150 (MQ=255)
                                                                catGCCTGGCGCAGGCGGAACGCAACGTTTAGTCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGATAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCt                                                                                       >  1:1993415/1‑150 (MQ=255)
                                                                  tGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCg                                                                                     >  1:512357/1‑150 (MQ=255)
                                                                  tGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCg                                                                                     <  1:1928839/150‑1 (MQ=255)
                                                                       ggCGCAGGCGGAACGCAACGTTTAATCCGTAGTGGCGGTAAATCGATAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGGACAGCAGGCCGGGCTGGTTAGAGACGTCTTCCCCTGCGATTTAACCCTCGAATAc                                                                                <  2:571937/150‑1 (MQ=255)
                                                                        gcgcAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACg                                                                               <  2:1058867/150‑1 (MQ=255)
                                                                        gcgcAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACg                                                                               <  2:808028/150‑1 (MQ=255)
                                                                          gcAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGcc                                                                             <  2:20173/150‑1 (MQ=255)
                                                                          gcAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGATAGCCACCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGcc                                                                             <  2:1163815/150‑1 (MQ=255)
                                                                           cAGGCGGAACGCAACGGTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCCGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACgagt                                                                            >  1:65882/1‑147 (MQ=255)
                                                                             ggCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTa                                                                          >  1:573238/1‑150 (MQ=255)
                                                                                 gAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGc                                                                      <  2:1364504/150‑1 (MQ=255)
                                                                                   aCGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTg                                                                    <  1:1981916/150‑1 (MQ=255)
                                                                                   aCGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCACAGCGATTTAACACTCGAATACGCCTTACAGCTg                                                                    >  2:583959/1‑150 (MQ=255)
                                                                                    cGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTgg                                                                   <  2:1866953/150‑1 (MQ=255)
                                                                                    cGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTgg                                                                   >  2:24086/1‑150 (MQ=255)
                                                                                     gCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCGAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGc                                                                  <  1:1091441/150‑1 (MQ=255)
                                                                                       aaCGTTTAATCCGTTGTGTCTGTAAATCGTTTGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCGCAGCAAGCACAGCAGGCCGGGCGGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCAt                                                                <  1:1910341/150‑1 (MQ=255)
                                                                                        aCGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGTAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATTCGCCTTTCAGCTGGCATc                                                               <  1:1016365/150‑1 (MQ=255)
                                                                                        aCGTGTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAACATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACTCCTTACAGCTGGCATc                                                               <  2:1904773/150‑1 (MQ=255)
                                                                                         cGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCg                                                              <  1:564833/150‑1 (MQ=255)
                                                                                                tCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATgg                                                       <  2:1331285/150‑1 (MQ=255)
                                                                                                  ggTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCa                                                     <  1:757831/149‑1 (MQ=255)
                                                                                                        gtCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCAc                                               >  2:1618629/1‑150 (MQ=255)
                                                                                                             tAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTcgcc                                          <  2:1800482/150‑1 (MQ=255)
                                                                                                                aTCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCt                                       <  2:1836208/150‑1 (MQ=255)
                                                                                                                  cGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGACTACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTgg                                     <  1:162051/150‑1 (MQ=255)
                                                                                                                   gTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGCCGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGc                                    >  1:1197406/1‑150 (MQ=255)
                                                                                                                   gTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGc                                    <  2:934087/150‑1 (MQ=255)
                                                                                                                    ttAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGcc                                   <  1:1909531/150‑1 (MQ=255)
                                                                                                                    ttAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGCTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGcc                                   <  2:744804/150‑1 (MQ=255)
                                                                                                                     tAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCt                                  >  1:1620383/1‑150 (MQ=255)
                                                                                                                      aGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCtt                                 <  2:2018104/150‑1 (MQ=255)
                                                                                                                      aGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCtt                                 <  2:590642/150‑1 (MQ=255)
                                                                                                                      aGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCtt                                 >  2:1928652/1‑150 (MQ=255)
                                                                                                                       gCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTa                                >  2:364455/1‑150 (MQ=255)
                                                                                                                        ccAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTAc                               <  2:1740124/150‑1 (MQ=255)
                                                                                                                         cAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACa                              <  2:1502386/150‑1 (MQ=255)
                                                                                                                         cAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACa                              <  1:1014975/150‑1 (MQ=255)
                                                                                                                           gCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAg                            >  1:877748/1‑150 (MQ=255)
                                                                                                                           gCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAg                            >  1:509395/1‑150 (MQ=255)
                                                                                                                           gCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAg                            >  1:33584/1‑150 (MQ=255)
                                                                                                                              aaaTGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCgg                         <  1:66991/150‑1 (MQ=255)
                                                                                                                              aaaTGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCgg                         <  1:529482/150‑1 (MQ=255)
                                                                                                                                   ttGCTGAGCGGTGATACTTTCTCCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAaag                    >  2:71318/2‑150 (MQ=255)
                                                                                                                                    tGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAaagc                   <  2:751778/150‑1 (MQ=255)
                                                                                                                                     gCTGAGCGGTGATACTTTCTCCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAaagca                  >  2:1719948/1‑150 (MQ=255)
                                                                                                                                     gCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTAAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAaagca                  >  1:890618/1‑150 (MQ=255)
                                                                                                                                      cTGAGCGGTGATACTTTCTCCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAaagcaa                 >  1:806466/1‑150 (MQ=255)
                                                                                                                                      cTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAaagcaa                 <  1:1126032/150‑1 (MQ=255)
                                                                                                                                       tGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGAAGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTAAAGCTGGAATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAaagcaag                >  2:273080/1‑150 (MQ=255)
                                                                                                                                        gAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAaagcaagc               >  2:1951993/1‑150 (MQ=255)
                                                                                                                                                 aaGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCa      >  1:1435871/1‑150 (MQ=255)
                                                                                                                                                  aGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAg     >  2:1460096/1‑150 (MQ=255)
                                                                                                                                                    tATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTc   >  2:580959/1‑150 (MQ=255)
                                                                                                                                                     aTCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCg  >  2:1620377/1‑150 (MQ=255)
                                                                                                                                                     aTCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCAAGTCg  >  2:410310/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGTGCGATGTGGTGGTTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCG  >  NC_000913/1457834‑1458132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: