Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,461,492 T→A 7.0% I200I (ATT→ATA paaJ → 3‑oxoadipyl‑CoA/3‑oxo‑5,6‑dehydrosuberyl‑CoA thiolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,461,4920TA 7.0% 399.4 / 10.3 136I200I (ATT→ATApaaJ3‑oxoadipyl‑CoA/3‑oxo‑5,6‑dehydrosuberyl‑CoA thiolase
Reads supporting (aligned to +/- strand):  ref base T (58/68);  new base A (3/7);  total (61/75)
Fisher's exact test for biased strand distribution p-value = 5.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

AACCCGCTCATGGCTCAGCAATTTGGAACTGACAGCATGCCGGAAACGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAACGACGCTGGAACAGTTACGTGGGTTAAAAGCACCATTTCGTGCCAATGGGGTGATTACCGC  >  NC_000913/1461343‑1461641
                                                                                                                                                     |                                                                                                                                                     
 aCCCGCTCATGGCTCAGCAATTTGGAACTGACAGCATGCCGGAAACGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCATTCATCAACGAACGGCAAAAGAGCAATACTCAGGAAtta                                                                                                                                                      >  1:283146/1‑149 (MQ=255)
 aCCCGCTCATGGCTCAGCAATTTGGAACTGACAGCATGCCGGAAACGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTAGCGCTACGCAGTCAGCACCGTACGGCAAAAGCGCAATCCTCAGGCATTc                                                                                                                                                      >  1:138617/1‑150 (MQ=255)
  cccGCTCATGGCTCAGCAATTTGGAACTGACAGCATGCCGGAAACGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCt                                                                                                                                                     >  1:842829/1‑150 (MQ=255)
  cccGCTCATGGCTCAGCAATTTGGAACTGACAGCATGCCGGAAACGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCt                                                                                                                                                     <  1:2004160/150‑1 (MQ=255)
   ccGCTCATGGCTCAGCAATTTGGAACTGACAGCATGCCGGAAACGGCAGAGAATGTAGCTGAACTGTTAAAAAACTCACGACAAGATCAAGATAGTTATGCGCTACGCAGTGAGCAACGTCCGGCAAACGCGCAATCCTCAGGCATACTg                                                                                                                                                    >  2:903133/1‑150 (MQ=255)
    cGCTCATGGCTCAGCAATTTGGAACTGACAGCATGCCGGAAACGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTgg                                                                                                                                                   <  2:1424325/150‑1 (MQ=255)
       tCATGGCTCAGCAATTTGGAACTGACAGCATGCCGGAAACGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTg                                                                                                                                                <  2:582093/150‑1 (MQ=255)
         aTGGCTCAGCAATTTGGAACTGACAGCATGCCGGAAACGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTgag                                                                                                                                              <  2:973110/150‑1 (MQ=255)
         aTGGCTCAGCAATTTGGAACTGACAGCATGCCGGAAACGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTgag                                                                                                                                              <  1:1518108/150‑1 (MQ=255)
           ggCTCAGCAATTTGGAACTGACAGCATGCCGGAAACGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTgagga                                                                                                                                            >  2:1099777/1‑150 (MQ=255)
            gCTCAGCAATTTGGAACTGACAGCATGCCGGAAACGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGAAAGTTTTGCGCTACGCAGTCAGCAACGTACGTCAAAAGCGCAATCCTCAGGCATTCAGGCTgaggag                                                                                                                                           >  2:1850800/1‑150 (MQ=255)
              tCAGCAATTTGGAACTGACAGCATGCCGGAAACGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCGTCAGGCATTCTGGCTGAGGAGAt                                                                                                                                         >  2:187239/1‑150 (MQ=255)
              tCAGCAATTTGGAACTGACAGCATGCCGGAAACGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGCCAGAATGCCTgaggattg                                                                                                                                         <  1:912815/150‑4 (MQ=255)
              tCAGCAATTTGGAACTGACAGCATGCCGGAAACGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGCCAGAATGCCTgaggattg                                                                                                                                         <  2:904738/150‑4 (MQ=255)
              tCAGCAATTTGGAACTGACAGCATGCCGGAAACGGCAGAGAATGTAGCTGAACTGTTAAAAAGCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGCCAGAATGCCTgaggattg                                                                                                                                         <  1:1434589/150‑4 (MQ=255)
              tCAGCAATTTGGAACTGACAGCATGCCGGAAACGGCAGAGAATGTAGCTGAAATGTTAAAAATCTCACGAGCAGATCAAGATAGTTTTGCGCTACGCTGTCAGCAACGTAAGGAAAAAGCGCAATCCTCAGCCAGAATGCCTgaggattg                                                                                                                                         <  2:1287044/150‑4 (MQ=255)
               cAGCAATTTGGAACTGACAGCATGCCGGAAACGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGAtt                                                                                                                                        >  1:1110845/1‑150 (MQ=255)
                aGCAATTTGGAACTGACAGCATGCCGGAAACGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGCCAGAATGCCTgaggattgcg                                                                                                                                       <  1:717447/150‑6 (MQ=255)
                  cAATTTGGAACTGACAGCATGCCGGAAACGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGtt                                                                                                                                     >  2:1938923/1‑150 (MQ=255)
                      ttGGAACTGACAGCATGCCGGAAACGTCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCgg                                                                                                                                 <  1:1313364/150‑1 (MQ=255)
                         gAACTGACAGCATGCCGGAAACGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTtg                                                                                                                              <  1:758872/150‑1 (MQ=255)
                           aCTGACAGCATGCCGGAAACGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTtgtg                                                                                                                            <  2:56467/150‑1 (MQ=255)
                            cTGACAGCATGCCGGAAACGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCCCGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGCTTGTTGCGGTTgtgt                                                                                                                           >  2:404198/1‑150 (MQ=255)
                             tGACAGCATGCCGGAAACGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGtt                                                                                                                          >  1:720830/1‑150 (MQ=255)
                             tGACAGCATGCCGGAAACGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGtt                                                                                                                          <  2:811662/150‑1 (MQ=255)
                              gACAGCATGCCGGAAACGGCAGAGAAAGTAGCTGAACTGTTAAAAATCTCACGAGAAGAGCATGATAGTTTTGCGCTACGCAGTCAGCAACGTACGCCAAAAGCGCAATCCTCAGGCTTTCTGGCTGAGGAGATTGTTCCGGTTGTGTTg                                                                                                                         >  2:1264813/1‑150 (MQ=255)
                              gACAGCAGGCCGGAAACGGCTGAGAATGTAGCTGAACTGTTAAAACTCTCACGAGAAGATCAAGTAAGTGTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTg                                                                                                                         <  1:1059916/150‑1 (MQ=255)
                               aCAGCATGCCGGAAACGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGa                                                                                                                        >  2:1170771/1‑150 (MQ=255)
                                  gCATGCCGGAAACGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGaaaa                                                                                                                     >  1:1340805/1‑150 (MQ=255)
                                     tGCCGGAAACGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCACCGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACa                                                                                                                  >  1:1216676/1‑150 (MQ=255)
                                     tGCCGGAAACGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACa                                                                                                                  >  2:646696/1‑150 (MQ=255)
                                     tGCCGGAAACGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACa                                                                                                                  >  1:1404889/1‑150 (MQ=255)
                                     tGCCGGAAACGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTAAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACa                                                                                                                  <  2:258136/150‑1 (MQ=255)
                                      gCCGGAAACGTCAGAGAATGTTGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACaa                                                                                                                 <  1:1202130/150‑1 (MQ=255)
                                      gCCGGAAACGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACaa                                                                                                                 <  2:1977033/150‑1 (MQ=255)
                                          gAAACGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGaaa                                                                                                             <  1:1402030/150‑1 (MQ=255)
                                           aaaCGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACTAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAg                                                                                                            <  1:1749081/150‑1 (MQ=255)
                                           aaaCGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAg                                                                                                            <  1:1810722/150‑1 (MQ=255)
                                              cGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGgtg                                                                                                         >  1:1580053/1‑150 (MQ=255)
                                              cGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGgtg                                                                                                         <  2:1649621/150‑1 (MQ=255)
                                                 cAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGtgttg                                                                                                      >  2:1721641/1‑150 (MQ=255)
                                                 cAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGtgttg                                                                                                      <  1:723010/150‑1 (MQ=255)
                                                   gagaATGTAGCTGAACTGTTAAAAATCTCACGAGATGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTa                                                                                                    >  2:710109/1‑150 (MQ=255)
                                                   gagaATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTa                                                                                                    <  1:1051528/150‑1 (MQ=255)
                                                      aaTGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACa                                                                                                 >  1:503621/1‑150 (MQ=255)
                                                       aTGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAg                                                                                                <  1:687518/150‑1 (MQ=255)
                                                           aGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAAt                                                                                            <  2:1527950/150‑1 (MQ=255)
                                                           aGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACAGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAAt                                                                                            >  2:748190/1‑150 (MQ=255)
                                                            gCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATa                                                                                           >  1:514674/1‑150 (MQ=255)
                                                             cTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATac                                                                                          >  1:1308377/1‑150 (MQ=255)
                                                                aaCTGTTAAAAATCTCAAGACTAGATCAAGATAGTTTTGCGCTACGCTGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATacaac                                                                                       <  1:334668/150‑1 (MQ=255)
                                                                    gTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACatga                                                                                   <  1:163166/150‑1 (MQ=255)
                                                                      tAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACatgatg                                                                                 >  2:123886/1‑150 (MQ=255)
                                                                      aaaaaaTCTCACGAGAAGATCAAGATAGTTTTGCTCTACGCAGTCAGCAACGTACGGCTAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACatgatg                                                                                 <  2:455535/149‑1 (MQ=255)
                                                                       aaaaaTCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACAtgatga                                                                                >  2:1150140/1‑150 (MQ=255)
                                                                       aaaaaTCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGACTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACAtgatga                                                                                >  2:1292282/1‑150 (MQ=255)
                                                                            tctcACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATc                                                                           <  1:1726740/150‑1 (MQ=255)
                                                                               cACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGACATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTgc                                                                        >  2:484276/1‑150 (MQ=255)
                                                                                    taagaTCAAGATAGTTTTGCTCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCTTTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCg                                                                   <  1:929596/149‑1 (MQ=255)
                                                                                       gaTCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGaa                                                                >  1:1462330/1‑150 (MQ=255)
                                                                                         tCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAACGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAac                                                              >  1:572752/1‑150 (MQ=255)
                                                                                         tCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAac                                                              <  2:1110845/150‑1 (MQ=255)
                                                                                            aGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAacgac                                                           <  1:68187/150‑1 (MQ=255)
                                                                                               tAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAACGACGCt                                                        <  1:620996/150‑1 (MQ=255)
                                                                                               tAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAACGACGCt                                                        <  1:1363418/150‑1 (MQ=255)
                                                                                                 gTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAACGACGCTgg                                                      <  2:1142470/150‑1 (MQ=255)
                                                                                                        gcTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAACGACGCTGGAACAGtt                                               <  2:1684292/150‑1 (MQ=255)
                                                                                                         cTACGCAGTCAGGAACGTACGGCAAAAGCGCAATCCTAAGGCATTCTGGCTGAGGAGATTGTTCCGTTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATAAAACATGATGAGCATCTGCGCCCTGAAACGACGCTGGAACAGTTa                                              <  1:1845585/150‑1 (MQ=255)
                                                                                                         cTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAACGACGCTGGAACAGTTa                                              <  1:157997/150‑1 (MQ=255)
                                                                                                         cTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAACGACGCTGGAACAGTTa                                              <  1:1118868/150‑1 (MQ=255)
                                                                                                              cAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTAGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAACGACGCTGGAACAGTTACGTgg                                         >  1:785984/1‑150 (MQ=255)
                                                                                                               aGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAACGACGCTGGAACAGTTACGTggg                                        <  2:714705/150‑1 (MQ=255)
                                                                                                                gTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAACGACGCTGGAACAGTTACGTGGGt                                       >  1:1164347/1‑150 (MQ=255)
                                                                                                                  cAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCTCCCGGAAACGACGCTGGAACAGTTACGTGGGTTa                                     <  1:222023/150‑1 (MQ=255)
                                                                                                                  cAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAACGACGCTGGAACAGTTACGTGGGTTa                                     >  1:1152198/1‑150 (MQ=255)
                                                                                                                    gCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAACGACGCTGGAACAGTTACGTGGGTTaaa                                   >  2:1965086/1‑150 (MQ=255)
                                                                                                                    gCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAACGACGCTCGAACAGTTACGTGGGTTaaa                                   >  1:258186/1‑150 (MQ=255)
                                                                                                                        cGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAACGACGCTGGAACAGTTACGTGGGTTAAAAGCa                               <  1:1107924/150‑1 (MQ=255)
                                                                                                                         gTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAACGACGCTGGAACAGTTACGTGGGTTAAAAGCAc                              <  1:463282/150‑1 (MQ=255)
                                                                                                                         gTACGGCAAAAGCGCAATCCTCAGGCATACTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAACGACGCTGGAACAGTTACGTGGGTTAAAAGCAc                              <  2:146069/150‑1 (MQ=255)
                                                                                                                          tACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAACGACGCTGGAACAGTTACGTGGGTTAAAAGCAcc                             >  1:1260167/1‑150 (MQ=255)
                                                                                                                             ggCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACACCATGATGCGCATCTGCGCCCGGAAACGCCGCTGGAACAGTTACGTGGGTTACAAGCACCAtt                          >  2:1482378/1‑150 (MQ=255)
                                                                                                                             ggCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAACGACGCTGGAACAGTTACGTGGGTTAAAAGCACCAtt                          >  2:111988/1‑150 (MQ=255)
                                                                                                                              gCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAACGACGCTGGAACAGTTACGTGGGTTAAAAGCACCAttt                         >  1:1532987/1‑150 (MQ=255)
                                                                                                                              gCAAAAGCGCAATCCTCACGCATTCTTGCTGAGGTTATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATTCAACATGATGAGCATCTGCGCCAGGAAACGACGCTGGAACAGTTACGTGGGTTAAAAGCACCAttt                         <  1:1330025/150‑1 (MQ=255)
                                                                                                                                  aaGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAACGACGCTGGAACAGTTACGTGGGTTAAAAGCACCATTTCGTg                     <  2:1758619/150‑1 (MQ=255)
                                                                                                                                   aGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAACGACGCTGGAACAGTTACGTGGGTTAAAAGCACCATTTCGTGc                    <  2:1404889/150‑1 (MQ=255)
                                                                                                                                     caatctCCTCAGCCAGAATGCCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAACGACGCTGGAACAGTTACGTGGGTTAAAAGCACCATTTCGTGCCa                  >  1:575558/6‑150 (MQ=255)
                                                                                                                                       cAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAACGACGCTGGAACAGTTACGTGGGTTAAAAGCACCATTTCGTGCCAAt                >  2:929566/1‑150 (MQ=255)
                                                                                                                                       cAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGAAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAACTACGCTGGAACAGTTACGTGGGTTAAAAGCACCATTTCGTGCCAAt                >  1:1057022/1‑150 (MQ=255)
                                                                                                                                         ctCCTCAGCCAGAATGCCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAACGACGCTGGAACAGTTACGTGGGTTAAACGCACCATTTCGTGCCAATgg              >  2:158152/2‑150 (MQ=255)
                                                                                                                                         aTCCTCCGGCATACTGGCTGAGGAGATTGTCCCGGTGGTGTTGAAAACCAAGCAAGCTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGCAACGACGCTGGAACAGGTACGTGGGTTAAAAGCACCATTTCGTGCCAATgg              <  2:279992/150‑1 (MQ=255)
                                                                                                                                           ccTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAACGACGCTGGAACAGTTACGTGGGTTTAAAGCACCATTTCGTGCCAATgggg            >  2:900944/1‑150 (MQ=255)
                                                                                                                                           ccTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAACGACGCTGGAACAGTTACGTGGGTTAAAAGCACCATTTCGTGCCAATgggg            >  2:1916227/1‑150 (MQ=255)
                                                                                                                                           ccTCAGGCAATCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACAAGATGAGCATCTGCGCCCGGAAACGACGCTGGAACAGTTACGTGGGTTAAAAGCACCATTTCGTGCCAATgggg            <  2:215999/150‑1 (MQ=255)
                                                                                                                                            cTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAACGACTCTGGAACAGTTACGTGGGTTAAAAGCAcc                             <  2:1178580/132‑1 (MQ=255)
                                                                                                                                            cTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAACGACGCTGGAACAGTTACGTGGGTTAAAAGCACCATTTCGTGCCAATGGGGt           >  1:1539009/1‑150 (MQ=255)
                                                                                                                                            cTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAACGACGCTGGAACAGTTACGTGGGTTAAAAGCACCATTTCGTGCCAATGGGGt           <  1:992656/150‑1 (MQ=255)
                                                                                                                                             tCAGGCTTTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAACGACGCTGGAACAGTTACGTGGGTTAAAAGCACCTTTTCGTGCCAATGGGGTg          <  2:138617/150‑1 (MQ=255)
                                                                                                                                               aGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAACGACGCTGGAACAGTTACGTGGGTTAAAAGCACCATTTCGTGCCAATGGGGTGAt        >  2:1038653/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AACCCGCTCATGGCTCAGCAATTTGGAACTGACAGCATGCCGGAAACGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGATCAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATTCTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATACAACATGATGAGCATCTGCGCCCGGAAACGACGCTGGAACAGTTACGTGGGTTAAAAGCACCATTTCGTGCCAATGGGGTGATTACCGC  >  NC_000913/1461343‑1461641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: