BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,517,484	C→G	5.5%	I32M (ATC→ATG)	ydcX →	DUF2566 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,517,484	0	C	G	5.5%	359.8 / 10.2	122	I32M (ATC→ATG)	ydcX	DUF2566 family protein
Reads supporting (aligned to +/- strand): ref base C (59/53); new base G (4/3); total (65/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.39e-01

Only 100 of 123 total aligned reads displayed.

AAAAAATGACGCATATTTGCGCGCGTTTTATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAG  >  NC_000913/1517336‑1517631

   aaaTGACGCATATTTGCGCGCGTTTTATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGtt                                                                                                                                                 >  2:284443/1‑150 (MQ=255)

     aTGACGCATATTTGCGCGAGTTTTATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTc                                                                                                                                               <  2:1444207/150‑1 (MQ=255)

      tGACGCATATTTGCGCGCGTTTTATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTGCTTTCTCACGATAAGAAACGCATCCGTTTCt                                                                                                                                              <  1:1236344/150‑1 (MQ=255)

         cGCATATTTGCGCGCGTTTTATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTaa                                                                                                                                           >  2:888305/1‑150 (MQ=255)

         cGCATATTTGCGCGCGTTTTATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTaa                                                                                                                                           >  1:2643/1‑150 (MQ=255)

         cGCATATTTGCGCGCGTTTTATTCATCTGGCTGGACGCCCGTACATGTCTCTATATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTaa                                                                                                                                           >  1:1887700/1‑150 (MQ=255)

          gCATATTTGCGCGCGTTTTATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAGACGCATCCGTTTCTTAAg                                                                                                                                          >  1:1160076/1‑150 (MQ=255)

          gCATATTTGCGCGCGTTTTATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAg                                                                                                                                          <  1:1568476/150‑1 (MQ=255)

           cATATTTGCGCGCGTTTTATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGGATGCGTTTCTTatcg                                                                                                                                         <  1:1856470/150‑4 (MQ=255)

                     cgcgTTTTATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCtgg                                                                                                                               >  1:1897828/1‑150 (MQ=255)

                     cgcgTCTTATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCtgg                                                                                                                               <  2:1945775/150‑1 (MQ=255)

                        gTTTTATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCtggtgg                                                                                                                            <  1:609503/150‑1 (MQ=255)

                         ttttATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTggg                                                                                                                           <  1:1363130/150‑1 (MQ=255)

                          tttATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTgggg                                                                                                                          <  2:749776/150‑1 (MQ=255)

                           ttATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTggggg                                                                                                                         >  1:1111918/1‑150 (MQ=255)

                            tATTCATCTGGCTGGACGCCCGTACATGTCTCTCTCTGAACACAGGCTTGCTTTTTATGCGGTTATGCCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGc                                                                                                                        <  2:348717/150‑1 (MQ=255)

                             aTTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTATTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTACTTAAGCGCCTTTCTGGTGGGGGcc                                                                                                                       >  2:1779072/1‑149 (MQ=255)

                               tCATCTGGCTGGACGCCAGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTAATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTATCTGGTGGGGGCAAc                                                                                                                     <  2:1057174/150‑1 (MQ=255)

                                cATCTGGCTGGACGCCCGTACATGTCTCTCTATCCACACATGCGTGTTGTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAATAAACGCATCCGTTTCTTAATCGCCTTTATGGTGGGGGCAACa                                                                                                                    >  2:208840/1‑150 (MQ=255)

                                cATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGATTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCATTTCTGGTGGGGGCAACa                                                                                                                    >  2:82933/1‑150 (MQ=255)

                                    tggctggACGCCCGTACATGTCTCTCTATCCACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTGCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGc                                                                                                                <  2:1750245/150‑1 (MQ=255)

                                    tggctggACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGc                                                                                                                <  1:1372415/150‑1 (MQ=255)

                                     ggctggACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGcc                                                                                                               >  1:552564/1‑150 (MQ=255)

                                      gctggCCGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCCATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCg                                                                                                              >  1:382694/1‑150 (MQ=255)

                                      gctggACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCg                                                                                                              <  1:807475/150‑1 (MQ=255)

                                        tggACGCACGGACATGTATCTCTATCAACTCATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGAt                                                                                                            <  2:1329794/150‑1 (MQ=255)

                                         ggACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATg                                                                                                           >  2:913742/1‑150 (MQ=255)

                                           aCGCCGGTACTTGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTGATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCAGCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAg                                                                                                         <  2:350684/150‑1 (MQ=255)

                                           aCGCCCGTTCATTTCTCTCTTTCAACACATGCTTGTTTTTTATGCGGGTATGGCAGCAATCTCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAg                                                                                                         <  2:1013821/150‑1 (MQ=255)

                                            cGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGt                                                                                                        >  2:728174/1‑150 (MQ=255)

                                             gCCCGTACATGGCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGtt                                                                                                       <  1:192141/150‑1 (MQ=255)

                                              cccGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGttt                                                                                                      >  2:1858789/1‑150 (MQ=255)

                                              cccGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGttt                                                                                                      <  1:747641/150‑1 (MQ=255)

                                              cccGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATAGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGttt                                                                                                      >  2:1851109/1‑150 (MQ=255)

                                                     aTGTCTCTCTCTCCACTCAGGCTGGGTATTTATGCGGGTATGGTAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCCCGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTg                                                                                               <  2:132576/150‑1 (MQ=255)

                                                     aTGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTg                                                                                               >  2:1972371/1‑150 (MQ=255)

                                                      tGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGGTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTgg                                                                                              <  1:985203/150‑1 (MQ=255)

                                                       gTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCAGCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGgc                                                                                             <  1:100143/150‑1 (MQ=255)

                                                         ctctctATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGgcgc                                                                                           >  1:406881/1‑150 (MQ=255)

                                                           ctctATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCtg                                                                                         >  2:727244/1‑150 (MQ=255)

                                                               aTCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCtgttgt                                                                                     >  2:262900/1‑150 (MQ=255)

                                                                tCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGAATTTCTTATCACCTGGTTACTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACAAGGCCGATGAGTTTCCCGGTGTCGCTgttgtt                                                                                    >  1:701052/1‑150 (MQ=255)

                                                                  aaCACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTATGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGtttt                                                                                  >  2:736624/1‑150 (MQ=255)

                                                                  aaCACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGtttt                                                                                  >  2:812746/1‑150 (MQ=255)

                                                                   acacATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCAGCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTc                                                                                 <  1:198073/150‑1 (MQ=255)

                                                                    cacaTGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATACGTTTCTTAATCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCa                                                                                >  1:1482711/1‑150 (MQ=255)

                                                                      caTGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACt                                                                              <  2:1216021/150‑1 (MQ=255)

                                                                         gCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGtt                                                                           >  1:1533686/1‑150 (MQ=255)

                                                                          cTTTTTTTTAATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGGTTCCCGGTGGCGCTGTTGTTTTCACTGttt                                                                          <  2:1518854/150‑1 (MQ=255)

                                                                          cTTGTTATTTATGAGCTTATGGCAGCAATCGCATTTCTTATCACCTGCTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCATTCTGGTGGGGGCAACATGGCAGAAGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGatt                                                                          >  2:1593975/1‑150 (MQ=255)

                                                                            tGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTAATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTACTTAACCGCCTTTCTGTTGGGGGCAACATGGCCTATTAGTTACCCGGTGGCGCTGTTTTTTTACATGTTTTa                                                                        >  2:1079433/1‑150 (MQ=255)

                                                                             gTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTaa                                                                       >  1:1256555/1‑150 (MQ=255)

                                                                                ttttATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTTGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTAACTGTTTTAAAta                                                                    >  2:1085700/1‑149 (MQ=255)

                                                                                ttttATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATc                                                                    >  2:1092731/1‑150 (MQ=255)

                                                                                ctttATGGGGTTATGGCCCCATACGCATTAGATCACCCCTGTAGTCATTCGCCCGATACTAACCGCATCCTTTTCTTATGCGCCTTTCTGGGGGGGTCAACATGGCCGATTAG‑TTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCt                                                                   <  2:1336679/149‑1 (MQ=255)

                                                                                  ttATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCtt                                                                  <  1:850838/150‑1 (MQ=255)

                                                                                    aTGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAACAAACGCATCCGGTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTAc                                                                <  1:1383686/150‑1 (MQ=255)

                                                                                        ggTTATGGCAGCAATCGCATTTCTTATAACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAATCTCCTTTCTGGTGGGGGAAACATGGAAGATGAGTTTCCCGGTGGCGCTGTTTTTTTAACTTTTTTAAATATTACTCAc                                                            >  1:1310041/1‑150 (MQ=255)

                                                                                          ttATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTg                                                          >  1:1693962/1‑150 (MQ=255)

                                                                                            aTGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGc                                                        >  2:1802474/1‑150 (MQ=255)

                                                                                               gcagcaATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTg                                                     <  2:1020383/150‑1 (MQ=255)

                                                                                                cagcaATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGa                                                    >  2:922615/1‑150 (MQ=255)

                                                                                                cagcaATCGCATTTCTTATCACCTGGTTTCTTTCTAACGATAAGAAACGCATCCTTTTCTTAAGATCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTATAAATCTTACTCACTGGCTTgc                                                    >  2:1185316/1‑149 (MQ=255)

                                                                                                  gcaATCGCATTTCTTATCATCTTGTTTATTTCTCACTATAATAAACGCATGCGTTTCTTAAGCGCCTTTATGGTTGGGGCAACATGGCAGATGAGTTTCCCGGTGTCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACg                                                  <  2:82698/150‑1 (MQ=255)

                                                                                                  gcaATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACg                                                  <  2:887212/150‑1 (MQ=255)

                                                                                                      tCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGcac                                              >  1:1454193/1‑150 (MQ=255)

                                                                                                       cGCATTTCTTATCCCCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCGTTATGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTTGCTTGACGGcacc                                             >  2:1753465/1‑150 (MQ=255)

                                                                                                       cGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGcacc                                             <  2:1849894/150‑1 (MQ=255)

                                                                                                             tCTTATCACCTGGTTTCTTTCTCACGCTAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAg                                       <  1:1790208/150‑1 (MQ=255)

                                                                                                             tCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTTAATCTTACTCACTGGCTTGACGGCACCACGCAg                                       >  1:1565519/1‑150 (MQ=255)

                                                                                                             tCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAg                                       <  1:1693184/150‑1 (MQ=255)

                                                                                                             tCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAg                                       <  1:391236/150‑1 (MQ=255)

                                                                                                               ttATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGAc                                     <  2:1053020/150‑1 (MQ=255)

                                                                                                               ttATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGAACCACGCAGAc                                     >  2:269769/1‑150 (MQ=255)

                                                                                                                   cACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTtat                                 >  2:1981691/1‑150 (MQ=255)

                                                                                                                   cACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTtaa                                 >  1:700342/1‑149 (MQ=255)

                                                                                                                   cACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCATTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTtat                                 <  1:284443/150‑1 (MQ=255)

                                                                                                                    gccTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTACTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGGTTTAAATCTAACTCACTGGCTTGACGGCACCACGCAGACGtatt                                >  1:1241651/2‑149 (MQ=255)

                                                                                                                     ccTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGCCGGCACCACGCAGACTTatat                               >  2:58761/1‑150 (MQ=255)

                                                                                                                      cTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATc                              >  1:1035594/1‑150 (MQ=255)

                                                                                                                             ctttctCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGa                       >  2:1478963/1‑150 (MQ=255)

                                                                                                                              tttctCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGAt                      >  2:586119/1‑150 (MQ=255)

                                                                                                                              tttctCACGATAAGAAACGCATCCGGTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGAt                      <  1:1116506/150‑1 (MQ=255)

                                                                                                                               ttctCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATg                     <  1:12300/150‑1 (MQ=255)

                                                                                                                               ttctCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTGTCCCGGTGGCGCTGTTGTTTTCACTGGGTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATg                     <  2:801791/150‑1 (MQ=255)

                                                                                                                                 ctcACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGaa                   >  2:1814603/1‑150 (MQ=255)

                                                                                                                                   cACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATc                 <  2:406881/150‑1 (MQ=255)

                                                                                                                                   cACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATc                 <  2:221497/150‑1 (MQ=255)

                                                                                                                                      cttAAGAAACGGATGCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGAt              >  2:682535/3‑150 (MQ=255)

                                                                                                                                        tAAGAAACGGATGCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATaa            >  1:654032/1‑150 (MQ=255)

                                                                                                                                        tAAGAAACGGATGCGTATCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGGTGTTGTTTTGACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACATATATCATATGGATGAATCGATat            >  2:780020/1‑149 (MQ=255)

                                                                                                                                         aaGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATaaa           <  2:2643/150‑1 (MQ=255)

                                                                                                                                           gAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCCCCACGCAGACTTATATCATTTGGATGAATCGATAAAtt         >  2:1535317/1‑150 (MQ=255)

                                                                                                                                             aaCGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAAttta       >  2:1868323/1‑149 (MQ=255)

                                                                                                                                             aaCGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTc       <  2:646418/150‑1 (MQ=255)

                                                                                                                                             aaCGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTc       >  1:1853295/1‑150 (MQ=255)

                                                                                                                                              aCGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTca      <  1:1858789/150‑1 (MQ=255)

                                                                                                                                              aCGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTca      <  1:932155/150‑1 (MQ=255)

                                                                                                                                               cGCATCCGTTTCTTAATCACCTTTCTGGTGGGTGCAACATCGGCGATGAGTTTCTCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGTCACCTCGCAGACTTATATCATTTGGATGAATCGATAAATTTcac     <  1:166977/150‑1 (MQ=255)

                                                                                                                                                  aTCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAg  >  2:448190/1‑150 (MQ=255)

AAAAAATGACGCATATTTGCGCGCGTTTTATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAG  >  NC_000913/1517336‑1517631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`