Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,613,276 G→T 6.3% intergenic (‑25/+39) glsB ← / ← sad glutaminase 2/succinate semialdehyde dehydrogenase, NAD(P)+‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,613,2760GT 6.3% 331.1 / 9.7 119intergenic (‑25/+39)glsB/sadglutaminase 2/succinate semialdehyde dehydrogenase, NAD(P)+‑dependent
Reads supporting (aligned to +/- strand):  ref base G (47/64);  new base T (5/3);  total (52/67)
Fisher's exact test for biased strand distribution p-value = 2.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.09e-01

Only 100 of 124 total aligned reads displayed.

CCCAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCACGCCACCAAAGGCCACTCGCGC  >  NC_000913/1613127‑1613425
                                                                                                                                                     |                                                                                                                                                     
cccAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTg                                                                                                                                                       <  2:1555871/150‑1 (MQ=255)
cccAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTg                                                                                                                                                       >  2:902777/1‑150 (MQ=255)
cccAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTg                                                                                                                                                       <  2:1761011/150‑1 (MQ=255)
   actCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGaaa                                                                                                                                                    <  1:159730/148‑1 (MQ=255)
    aTCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGaaaa                                                                                                                                                   >  1:480558/1‑150 (MQ=255)
       gggAACCGTCTGCTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGAc                                                                                                                                                <  1:1823240/150‑1 (MQ=255)
        ggAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGAcc                                                                                                                                               <  1:1204059/150‑1 (MQ=255)
            ccGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCg                                                                                                                                           <  2:1599411/150‑1 (MQ=255)
            ccGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCg                                                                                                                                           <  1:1804298/150‑1 (MQ=255)
            ccGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCAGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGAACTGCg                                                                                                                                           >  1:961470/1‑150 (MQ=255)
             cGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTTTTCAGACCTGCgg                                                                                                                                          <  2:1502259/150‑2 (MQ=255)
              gTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATAGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAg                                                                                                                                         >  1:1545159/1‑150 (MQ=255)
               tCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCATTGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTTTTCAGACCTGCggtc                                                                                                                                        <  1:174916/150‑4 (MQ=255)
                 tACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGtat                                                                                                                                      >  1:454472/1‑150 (MQ=255)
                  aCTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCGATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGtata                                                                                                                                     <  2:57074/150‑1 (MQ=255)
                  aCTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGtata                                                                                                                                     <  2:759613/150‑1 (MQ=255)
                  aCTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGtata                                                                                                                                     <  1:1108232/150‑1 (MQ=255)
                  aCTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCCGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGtata                                                                                                                                     >  2:2014840/1‑150 (MQ=255)
                    tGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATc                                                                                                                                   <  1:1521601/150‑1 (MQ=255)
                        gCCAGCGCCGGATTATAATCCGCGACTTTACCCTGACGAATGAGCGGCGGCACTTGCCGCATGATGTTTTCTAAAATTGCATTATCCATGGCGCCTGCCCCGTTCTACTCCGGGCCCGCAGGGCTGAAATGACCTGCGAGTTTATCagag                                                                                                                               >  2:1309535/1‑150 (MQ=255)
                         ccAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTATGAAAAGACCTGCGAGTATATCAGAGc                                                                                                                              <  2:1533476/150‑1 (MQ=255)
                          cAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCAGCAAGATGTTTTCTAAAATTGCATTATACATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCt                                                                                                                             >  2:114015/1‑150 (MQ=255)
                           aGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTg                                                                                                                            >  1:378918/1‑150 (MQ=255)
                            gcgcCGGATTATAATCCGCGACTTTACCCTGACCATTGAGCGGCCGCACTTGCGGCAAGATGTTTTCTTATATTGCATTATCCATGGCGACGGCCACTTTCTACTCCGGGACCGCAGGTCTGAATAGACCTGCGAGTATATCAGAGCTGa                                                                                                                           >  1:46743/1‑150 (MQ=255)
                              gcCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAat                                                                                                                         <  1:1401447/150‑1 (MQ=255)
                               ccGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAata                                                                                                                        >  1:856183/1‑150 (MQ=255)
                                        aaTCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCATGATGTTTTCTAAAATTGCATTCTCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTc                                                                                                               <  2:1916341/150‑1 (MQ=255)
                                          tCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATTTCAGAGCTGAATATGTCGCGTCAg                                                                                                             >  1:382919/1‑150 (MQ=255)
                                           ccGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTCCTCCTGGACCGCCGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGa                                                                                                            >  1:1085297/1‑150 (MQ=255)
                                           ccGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGACACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGa                                                                                                            >  1:1145541/1‑150 (MQ=255)
                                           ccGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGAAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGACACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTAGCGTCAGa                                                                                                            >  1:1153092/1‑150 (MQ=255)
                                              cgACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATcc                                                                                                         <  2:754337/150‑1 (MQ=255)
                                                 cTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGt                                                                                                      <  1:1439173/150‑1 (MQ=255)
                                                    tACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCtt                                                                                                   <  2:784477/150‑1 (MQ=255)
                                                      cccTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTc                                                                                                 <  1:1079247/150‑1 (MQ=255)
                                                      cccTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTc                                                                                                 <  1:1298235/150‑1 (MQ=255)
                                                      cccTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTc                                                                                                 <  1:592523/150‑1 (MQ=255)
                                                        cTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCca                                                                                               <  2:1226891/150‑1 (MQ=255)
                                                         tGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCcac                                                                                              <  2:497825/150‑1 (MQ=255)
                                                         tGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCcac                                                                                              <  2:589806/150‑1 (MQ=255)
                                                           acgaaTGAGCGGACGCACTTGCCGCAAGATGTTTTCTAAATTTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCacac                                                                                            <  1:171653/147‑1 (MQ=255)
                                                            ccAATGAGCGGCCGCACTTGCCGCAAGATGATTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGGATATGAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCGACAcc                                                                                           >  1:1013989/1‑150 (MQ=255)
                                                             cAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCg                                                                                          >  1:1501355/1‑150 (MQ=255)
                                                                tGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCt                                                                                       <  1:1048617/150‑1 (MQ=255)
                                                                  aGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGATTATGTCGCGTCAGAGCCGGTGTTTCCACACCGTCTgg                                                                                     >  2:365788/1‑150 (MQ=255)
                                                                    cGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGCCGCGTCAGATCCGGTCTTTCCACACCGTCTGGat                                                                                   <  2:828834/150‑1 (MQ=255)
                                                                     ggCCGCTCTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGata                                                                                  <  2:280474/150‑1 (MQ=255)
                                                                     ggCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGata                                                                                  <  1:1735043/150‑1 (MQ=255)
                                                                      gCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGatat                                                                                 >  2:1682628/1‑150 (MQ=255)
                                                                        cGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTa                                                                               >  1:1589274/1‑150 (MQ=255)
                                                                        cGCACTTGCCGCAAGATGTTTTCTAAAATTGCAATATCCATGGCGACTGCCACTTTCTACTCCTGGACCTCAGGTCTTAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTa                                                                               <  1:1319533/150‑1 (MQ=255)
                                                                         gctcTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTAc                                                                              <  1:1353457/147‑1 (MQ=255)
                                                                         gCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTAc                                                                              <  1:445124/150‑1 (MQ=255)
                                                                         gCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTAc                                                                              <  1:1678702/150‑1 (MQ=255)
                                                                           aCTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGGCTTTCCACACCGTCTGGATATTACAg                                                                            <  2:1631580/150‑1 (MQ=255)
                                                                               gCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAAtt                                                                        <  1:1918922/150‑1 (MQ=255)
                                                                               gCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAAtt                                                                        >  1:249429/1‑150 (MQ=255)
                                                                                ccGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTc                                                                       <  2:1826632/150‑1 (MQ=255)
                                                                                ccGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTc                                                                       >  1:811731/1‑150 (MQ=255)
                                                                                 cgaaaGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCg                                                                      <  1:906649/147‑1 (MQ=255)
                                                                                 cGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCg                                                                      >  1:1869215/1‑150 (MQ=255)
                                                                                  gCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCgt                                                                     <  2:1945198/150‑1 (MQ=255)
                                                                                  gCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCGACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCgt                                                                     >  1:828634/1‑150 (MQ=255)
                                                                                   cAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCgtg                                                                    >  2:1829735/1‑150 (MQ=255)
                                                                                        tGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGc                                                               <  1:233567/150‑1 (MQ=255)
                                                                                            ttCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGaa                                                           >  2:1969819/1‑150 (MQ=255)
                                                                                               tAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATg                                                        <  1:660835/150‑1 (MQ=255)
                                                                                                aaaaTTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATgg                                                       >  2:1254626/1‑150 (MQ=255)
                                                                                                  aaTTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGa                                                     <  1:746958/150‑1 (MQ=255)
                                                                                                   aTTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGaa                                                    <  2:1297326/150‑1 (MQ=255)
                                                                                                      gCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGc                                                 <  2:1776935/150‑1 (MQ=255)
                                                                                                          tATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAATGCTCAc                                             <  2:825659/150‑1 (MQ=255)
                                                                                                           aTCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACg                                            <  1:1495216/150‑1 (MQ=255)
                                                                                                             ccATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGAc                                          <  1:836469/150‑1 (MQ=255)
                                                                                                                  gCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAg                                     <  1:71992/150‑1 (MQ=255)
                                                                                                                     aCTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCa                                  >  2:1470476/1‑150 (MQ=255)
                                                                                                                      cTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCAc                                 <  2:1144438/150‑1 (MQ=255)
                                                                                                                      cTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCAc                                 <  2:1178389/150‑1 (MQ=255)
                                                                                                                              ttCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGTGAAAGCTCACGACCAAAGCCACTCTTTTTc                         <  2:1501355/150‑1 (MQ=255)
                                                                                                                              ttCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTc                         <  1:1498957/150‑1 (MQ=255)
                                                                                                                              ttCTACTCCTAGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTc                         <  1:1078949/150‑1 (MQ=255)
                                                                                                                                ctactaCTGGAACGCAGGTCTGAAAATAACTGCGAGTATATCATAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCAc                       <  2:850184/150‑1 (MQ=255)
                                                                                                                                cTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCAc                       >  2:1399863/1‑150 (MQ=255)
                                                                                                                                cTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCAc                       >  1:882206/1‑150 (MQ=255)
                                                                                                                                 tACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGAGTCAGATCCGGTCTTTCCACACCGTATGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACACTTTTTCACg                      >  1:899182/1‑150 (MQ=255)
                                                                                                                                   cTCCTGGACCGCAGGTCTGAAAAGAAATGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCAGGTCTTTCCCCACCGTCTGGATATTACAGAATTCGTGTAAGTCGAAATGCGTAAGCTCACGACCAAAGCCACTCTTTTTCACGcc                    >  2:387414/1‑150 (MQ=255)
                                                                                                                                      cTGGACCGCAGGTCTGTAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCACGccacc                 <  2:1869215/150‑1 (MQ=255)
                                                                                                                                      cTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCACGccacc                 >  1:1336240/1‑150 (MQ=255)
                                                                                                                                          ctcGCAGGTCTTTTCAGACCTGCGAGTATATCAGAGCTGATTATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCAGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCACGCCACCAAAg             >  1:239840/3‑150 (MQ=255)
                                                                                                                                          ctcGCAGGTCTTTTCAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGATCAAAGCCACTCTGTTTCCCGCCACCAAAg             >  2:1231746/3‑150 (MQ=255)
                                                                                                                                          ctcGCAGGTCTTTTCAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCACGCCACCAAAg             >  1:266715/3‑150 (MQ=255)
                                                                                                                                          aCCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCACGCCACCAAAg             <  1:1551129/150‑1 (MQ=255)
                                                                                                                                          aCCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCACGCCACCAAAg             >  1:1632076/1‑150 (MQ=255)
                                                                                                                                           tcGCAGGTCTTTTCAGACCTGCGAATATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCACGCCACCAAAgg            >  1:1386788/2‑150 (MQ=255)
                                                                                                                                           ccGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCACGCCACCAAAgg            >  1:438641/1‑150 (MQ=255)
                                                                                                                                           ccGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCACGCCACCAAAgg            >  2:724509/1‑150 (MQ=255)
                                                                                                                                               aGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCACGCCACCAAAGGCCAc        <  2:642940/150‑1 (MQ=255)
                                                                                                                                                ggTCGGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCACGCCACCAAAGGCCACt       <  1:1349027/150‑1 (MQ=255)
                                                                                                                                                    tGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTAACGCCACCAAAGGCCACTcgcg   >  2:296476/1‑150 (MQ=255)
                                                                                                                                                     gAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCACGCCACCAAAGGCCACTcgcgc  >  2:1608873/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCCAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCACGCCACCAAAGGCCACTCGCGC  >  NC_000913/1613127‑1613425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: