Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,613,279 A→C 5.9% intergenic (‑28/+36) glsB ← / ← sad glutaminase 2/succinate semialdehyde dehydrogenase, NAD(P)+‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,613,2790AC 5.9% 370.1 / 10.5 119intergenic (‑28/+36)glsB/sadglutaminase 2/succinate semialdehyde dehydrogenase, NAD(P)+‑dependent
Reads supporting (aligned to +/- strand):  ref base A (46/64);  new base C (5/2);  total (52/67)
Fisher's exact test for biased strand distribution p-value = 2.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.07e-01

Only 100 of 121 total aligned reads displayed.

AATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCACGCCACCAAAGGCCACTCGCGC  >  NC_000913/1613130‑1613425
                                                                                                                                                     |                                                                                                                                                  
actCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGaaa                                                                                                                                                    <  1:159730/148‑1 (MQ=255)
 aTCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGaaaa                                                                                                                                                   >  1:480558/1‑150 (MQ=255)
   cGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGa                                                                                                                                                 >  2:1472271/1‑150 (MQ=255)
    gggAACCGTCTGCTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGAc                                                                                                                                                <  1:1823240/150‑1 (MQ=255)
     ggAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGAcc                                                                                                                                               <  1:1204059/150‑1 (MQ=255)
         ccGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCg                                                                                                                                           <  2:1599411/150‑1 (MQ=255)
         ccGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCg                                                                                                                                           <  1:1804298/150‑1 (MQ=255)
          cGTCTACTGTAGCCAGCTCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGa                                                                                                                                          <  1:644159/150‑1 (MQ=255)
          cGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTTTTCAGACCTGCgg                                                                                                                                          <  2:1502259/150‑2 (MQ=255)
           gTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATAGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAg                                                                                                                                         >  1:1545159/1‑150 (MQ=255)
            tCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCATTGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTTTTCAGACCTGCggtc                                                                                                                                        <  1:174916/150‑4 (MQ=255)
             cTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGta                                                                                                                                       <  2:412500/150‑1 (MQ=255)
              tACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGtat                                                                                                                                      >  1:454472/1‑150 (MQ=255)
               aCTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCGATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGtata                                                                                                                                     <  2:57074/150‑1 (MQ=255)
               aCTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGtata                                                                                                                                     <  2:759613/150‑1 (MQ=255)
               aCTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGtata                                                                                                                                     <  1:1108232/150‑1 (MQ=255)
               aCTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCCGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGtata                                                                                                                                     >  2:2014840/1‑150 (MQ=255)
                 tGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATc                                                                                                                                   <  1:1521601/150‑1 (MQ=255)
                     gCCAGCGCCGGATTATAATCCGCGACTTTACCCTGACGAATGAGCGGCGGCACTTGCCGCATGATGTTTTCTAAAATTGCATTATCCATGGCGCCTGCCCCGTTCTACTCCGGGCCCGCAGGGCTGAAATGACCTGCGAGTTTATCagag                                                                                                                               >  2:1309535/1‑150 (MQ=255)
                      ccAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTATGAAAAGACCTGCGAGTATATCAGAGc                                                                                                                              <  2:1533476/150‑1 (MQ=255)
                       cAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCt                                                                                                                             >  2:1894615/1‑150 (MQ=255)
                       cAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCAGCAAGATGTTTTCTAAAATTGCATTATACATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCt                                                                                                                             >  2:114015/1‑150 (MQ=255)
                        aGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTg                                                                                                                            >  1:378918/1‑150 (MQ=255)
                         gcgcCGGATTATAATCCGCGACTTTACCCTGACCATTGAGCGGCCGCACTTGCGGCAAGATGTTTTCTTATATTGCATTATCCATGGCGACGGCCACTTTCTACTCCGGGACCGCAGGTCTGAATAGACCTGCGAGTATATCAGAGCTGa                                                                                                                           >  1:46743/1‑150 (MQ=255)
                           gcCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAat                                                                                                                         <  1:1401447/150‑1 (MQ=255)
                            ccGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAata                                                                                                                        >  1:856183/1‑150 (MQ=255)
                                   attaTCCGCGACTTTACCCTGAGGAATGAGCGGCCGCACTTGCCGAAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCGTGGACCGCAGGTCTGAATAGACCTGCGAGTATATCAGAGCTGAATATGTcgcg                                                                                                                 <  1:1617159/147‑1 (MQ=255)
                                      aTCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCa                                                                                                              >  1:906564/1‑150 (MQ=255)
                                       tCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATTTCAGAGCTGAATATGTCGCGTCAg                                                                                                             >  1:382919/1‑150 (MQ=255)
                                        ccGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTCCTCCTGGACCGCCGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGa                                                                                                            >  1:1085297/1‑150 (MQ=255)
                                        ccGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGACACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGa                                                                                                            >  1:1145541/1‑150 (MQ=255)
                                        ccGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGAAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGACACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTAGCGTCAGa                                                                                                            >  1:1153092/1‑150 (MQ=255)
                                           cgACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATcc                                                                                                         <  2:754337/150‑1 (MQ=255)
                                             aCTTTACCCTGTCCAATGAGCTGACGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCgg                                                                                                       <  2:1737135/150‑1 (MQ=255)
                                              cTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGt                                                                                                      <  1:1439173/150‑1 (MQ=255)
                                                   cccTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTc                                                                                                 <  1:592523/150‑1 (MQ=255)
                                                   cccTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTc                                                                                                 <  1:1079247/150‑1 (MQ=255)
                                                   cccTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTc                                                                                                 >  2:657819/1‑150 (MQ=255)
                                                      tGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCcac                                                                                              <  2:497825/150‑1 (MQ=255)
                                                        acgaaTGAGCGGACGCACTTGCCGCAAGATGTTTTCTAAATTTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCacac                                                                                            <  1:171653/147‑1 (MQ=255)
                                                         ccAATGAGCGGCCGCACTTGCCGCAAGATGATTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGGATATGAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCGACAcc                                                                                           >  1:1013989/1‑150 (MQ=255)
                                                          cAATGAGCGGCCGCACTTGCCTCAAGATGTTTTATAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCAGCGATTATATCAGAGCTGAATATGTAGCGTCAGATCCGGACTGTCCACGCCg                                                                                          >  1:134926/1‑150 (MQ=255)
                                                            aTGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCATGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTc                                                                                        >  1:1800525/1‑150 (MQ=255)
                                                             tGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCt                                                                                       <  1:1048617/150‑1 (MQ=255)
                                                               aGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGATTATGTCGCGTCAGAGCCGGTGTTTCCACACCGTCTgg                                                                                     >  2:365788/1‑150 (MQ=255)
                                                                 cGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGCCGCGTCAGATCCGGTCTTTCCACACCGTCTGGat                                                                                   <  2:828834/150‑1 (MQ=255)
                                                                  ggCCGCTCTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGata                                                                                  <  2:280474/150‑1 (MQ=255)
                                                                   gCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGatat                                                                                 >  2:1682628/1‑150 (MQ=255)
                                                                    ccGGACTTGCCGGAAGTTGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTTCTCCTGGACCGCAGGTCTGAAAAGTCCTGCGAGTATATCAGTGCTGATTATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATAtt                                                                                <  1:1986484/150‑1 (MQ=255)
                                                                     cGCACTTGCCGCAAGATGTTTTCTAAAATTGCAATATCCATGGCGACTGCCACTTTCTACTCCTGGACCTCAGGTCTTAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTa                                                                               <  1:1319533/150‑1 (MQ=255)
                                                                      gctcTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTAc                                                                              <  1:1353457/147‑1 (MQ=255)
                                                                      gCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTAc                                                                              <  1:445124/150‑1 (MQ=255)
                                                                      gCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTAc                                                                              <  1:1678702/150‑1 (MQ=255)
                                                                        aCTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGGCTTTCCACACCGTCTGGATATTACAg                                                                            <  2:1631580/150‑1 (MQ=255)
                                                                         cTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATTTTACAGa                                                                           >  2:1282286/1‑150 (MQ=255)
                                                                            gCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAAtt                                                                        >  1:249429/1‑150 (MQ=255)
                                                                             ccGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTc                                                                       <  2:1826632/150‑1 (MQ=255)
                                                                             ccGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTc                                                                       >  1:811731/1‑150 (MQ=255)
                                                                              cgaaaGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCg                                                                      <  1:906649/147‑1 (MQ=255)
                                                                              cGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCg                                                                      >  1:1869215/1‑150 (MQ=255)
                                                                               gCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCgt                                                                     <  2:1945198/150‑1 (MQ=255)
                                                                               gCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCGACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCgt                                                                     >  1:828634/1‑150 (MQ=255)
                                                                                cAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCgtg                                                                    >  2:1829735/1‑150 (MQ=255)
                                                                                     tGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGc                                                               <  1:233567/150‑1 (MQ=255)
                                                                                         ttCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGaa                                                           >  2:1969819/1‑150 (MQ=255)
                                                                                             aaaaTTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATgg                                                       >  2:1254626/1‑150 (MQ=255)
                                                                                               aaTTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGa                                                     <  1:746958/150‑1 (MQ=255)
                                                                                                aTTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGaa                                                    <  2:1297326/150‑1 (MQ=255)
                                                                                                     aTTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTc                                               <  1:813591/150‑1 (MQ=255)
                                                                                                       tATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAATGCTCAc                                             <  2:825659/150‑1 (MQ=255)
                                                                                                        aTCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACg                                            <  1:1495216/150‑1 (MQ=255)
                                                                                                               gCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAg                                     <  1:71992/150‑1 (MQ=255)
                                                                                                                 gACTGCCACTTTCTACTCCTGGACCGCAGGTCGGAAAAGACCTGCGAGTAAATCAGAGCTGAATATGTCGCGTCAGATCCGGGCTTTCCACACCGTCAGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGcc                                   <  1:1217446/150‑1 (MQ=255)
                                                                                                                  aCTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCa                                  >  2:1470476/1‑150 (MQ=255)
                                                                                                                   cTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCAc                                 <  2:1144438/150‑1 (MQ=255)
                                                                                                                   cTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCAc                                 <  2:1178389/150‑1 (MQ=255)
                                                                                                                   cTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCAc                                 <  2:1152059/150‑1 (MQ=255)
                                                                                                                           ttCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGTGAAAGCTCACGACCAAAGCCACTCTTTTTc                         <  2:1501355/150‑1 (MQ=255)
                                                                                                                           ttCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTc                         <  1:1498957/150‑1 (MQ=255)
                                                                                                                           ttCTACTCCTAGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTc                         <  1:1078949/150‑1 (MQ=255)
                                                                                                                             ctactaCTGGAACGCAGGTCTGAAAATAACTGCGAGTATATCATAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCAc                       <  2:850184/150‑1 (MQ=255)
                                                                                                                             cTACTCCTGGACGGCATGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGGCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCAc                       <  2:794892/150‑1 (MQ=255)
                                                                                                                             cTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCAc                       >  1:882206/1‑150 (MQ=255)
                                                                                                                              tACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGAGTCAGATCCGGTCTTTCCACACCGTATGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACACTTTTTCACg                      >  1:899182/1‑150 (MQ=255)
                                                                                                                                cTCCTGGACCGCAGGTCTGAAAAGAAATGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCAGGTCTTTCCCCACCGTCTGGATATTACAGAATTCGTGTAAGTCGAAATGCGTAAGCTCACGACCAAAGCCACTCTTTTTCACGcc                    >  2:387414/1‑150 (MQ=255)
                                                                                                                                   cTGGACCGCAGGTCTGTAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCACGccacc                 <  2:1869215/150‑1 (MQ=255)
                                                                                                                                   cTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCACGccacc                 >  1:1336240/1‑150 (MQ=255)
                                                                                                                                       ctcGCAGGTCTTTTCAGACCTGCGAGTATATCAGAGCTGATTATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCAGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCACGCCACCAAAg             >  1:239840/3‑150 (MQ=255)
                                                                                                                                       ctcGCAGGTCTTTTCAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCCCACCGTCTGGATATTACAGAATTCGTGTAAGACGAAATGGGAAAGCTCACGATCAAAGCCACTCTTTTTCACGCCAGCACAg             >  2:264228/3‑150 (MQ=255)
                                                                                                                                       ctcGCAGGTCTTTTCAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGATCAAAGCCACTCTGTTTCCCGCCACCAAAg             >  2:1231746/3‑150 (MQ=255)
                                                                                                                                       ctcGCAGGTCTTTTCAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCACGCCACCAAAg             >  1:266715/3‑150 (MQ=255)
                                                                                                                                       aCCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCACGCCACCAAAg             <  1:1551129/150‑1 (MQ=255)
                                                                                                                                       aCCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCACGCCACCAAAg             >  1:1632076/1‑150 (MQ=255)
                                                                                                                                        ccGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCACGCCACCAAAgg            >  2:724509/1‑150 (MQ=255)
                                                                                                                                        ccGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCACGCCACCAAAgg            >  1:438641/1‑150 (MQ=255)
                                                                                                                                            aGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCACGCCACCAAAGGCCAc        <  2:642940/150‑1 (MQ=255)
                                                                                                                                             ggTCGGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCACGCCACCAAAGGCCACt       <  1:1349027/150‑1 (MQ=255)
                                                                                                                                                cTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCACGCCACCAAAGGCCACTcgc    >  1:1534189/1‑150 (MQ=255)
                                                                                                                                                  gAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCACGCCACCAAAGGCCACTcgcgc  <  1:1472271/150‑1 (MQ=255)
                                                                                                                                                  gAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCACGCCACCAAAGGCCACTcgcgc  >  2:1608873/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
AATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAGATGTTTTCTAAAATTGCATTATCCATGGCGACTGCCACTTTCTACTCCTGGACCGCAGGTCTGAAAAGACCTGCGAGTATATCAGAGCTGAATATGTCGCGTCAGATCCGGTCTTTCCACACCGTCTGGATATTACAGAATTCGTGTAAGCCGAAATGGGAAAGCTCACGACCAAAGCCACTCTTTTTCACGCCACCAAAGGCCACTCGCGC  >  NC_000913/1613130‑1613425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: