Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,657,895 A→T 5.2% intergenic (+25/‑174) ynfD → / → ynfE DUF1161 family periplasmic protein/putative selenate reductase, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,657,8950AT 5.2% 413.8 / 7.0 140intergenic (+25/‑174)ynfD/ynfEDUF1161 family periplasmic protein/putative selenate reductase, periplasmic
Reads supporting (aligned to +/- strand):  ref base A (65/67);  new base T (3/5);  total (68/72)
Fisher's exact test for biased strand distribution p-value = 7.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01

Only 100 of 144 total aligned reads displayed.

TTCCCAGGTCGTCGGCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAATATATAAACTTTTATA  >  NC_000913/1657747‑1658042
                                                                                                                                                    |                                                                                                                                                   
ttCCCAGGTCGTCGGCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGatat                                                                                                                                                    >  2:752170/1‑150 (MQ=255)
  cccAGGTCGTCGGCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTACCGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGCACCGCAGTAACGCATTATCGACTGAACGCCGGATATgc                                                                                                                                                  >  2:762354/1‑149 (MQ=255)
   ccAGGTCGTCGGCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGAc                                                                                                                                                 <  2:648024/150‑1 (MQ=255)
   ccAGGTCGTCGGCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGAc                                                                                                                                                 <  1:752170/150‑1 (MQ=255)
         cgtcgGCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTCGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATcc                                                                                                                                           >  1:528125/1‑150 (MQ=255)
            cgGCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATTTGTCATATCCGGc                                                                                                                                        <  1:1407966/150‑1 (MQ=255)
            cgGCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATTTGTCATATCCGGc                                                                                                                                        <  1:9042/150‑1 (MQ=255)
            cgGCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGc                                                                                                                                        <  2:808724/150‑1 (MQ=255)
            cgGCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGc                                                                                                                                        <  2:1533636/150‑1 (MQ=255)
            cgGACATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGc                                                                                                                                        <  2:1085385/150‑1 (MQ=255)
              gCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATTTGTCATATCCGGCgt                                                                                                                                      <  2:1221768/150‑2 (MQ=255)
              gCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGa                                                                                                                                      >  1:1895404/1‑150 (MQ=255)
                 aTTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGAttt                                                                                                                                   >  1:573555/1‑150 (MQ=255)
                  ttGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTg                                                                                                                                  <  2:1008858/150‑1 (MQ=255)
                  ttGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTg                                                                                                                                  <  2:1097732/150‑1 (MQ=255)
                     cgcTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAAc                                                                                                                               <  1:755009/150‑1 (MQ=255)
                     cgcTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAAc                                                                                                                               <  2:814699/150‑1 (MQ=255)
                        tAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATa                                                                                                                            <  2:984003/150‑1 (MQ=255)
                               aCGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATa                                                                                                                     <  1:1888601/150‑1 (MQ=255)
                                 gCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCATGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAAt                                                                                                                   >  1:1463524/1‑150 (MQ=255)
                                   aTAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATcc                                                                                                                 >  1:692067/1‑150 (MQ=255)
                                   aTAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATcc                                                                                                                 <  1:769946/150‑1 (MQ=255)
                                    tAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATccc                                                                                                                >  1:1880470/1‑150 (MQ=255)
                                              tataCCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTAc                                                                                                      >  1:1348083/1‑150 (MQ=255)
                                                taCCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTc                                                                                                    <  2:1697561/150‑1 (MQ=255)
                                                 aCCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCa                                                                                                   <  2:681188/150‑1 (MQ=255)
                                                  cccGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCTGCGATTTGAACATACAACATAATCCCACCTTATTACTCAt                                                                                                  >  1:1567822/1‑150 (MQ=255)
                                                  cccGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCAt                                                                                                  >  1:1904091/1‑150 (MQ=255)
                                                  cccGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCAt                                                                                                  >  2:135090/1‑150 (MQ=255)
                                                  cccGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCAt                                                                                                  >  2:1174779/1‑150 (MQ=255)
                                                   ccGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATa                                                                                                 >  1:186687/1‑150 (MQ=255)
                                                     gCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATAcc                                                                                               <  1:1557372/150‑1 (MQ=255)
                                                                 gTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGata                                                                                   <  2:174160/150‑1 (MQ=255)
                                                                   aaCGTCTCTGCTCCCGCGCAGGCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATg                                                                                 <  1:1768786/150‑1 (MQ=255)
                                                                      gTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGAt                                                                              <  1:832847/150‑1 (MQ=255)
                                                                       tctcTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGAtt                                                                             <  1:1676158/150‑1 (MQ=255)
                                                                       tctcTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGAtt                                                                             <  1:1986614/150‑1 (MQ=255)
                                                                          ctGCTCCCGCGCAGTCTAGCCAGGATGGCGCTCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATTACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATtaat                                                                          <  1:376876/150‑1 (MQ=255)
                                                                          ctGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATtaat                                                                          >  1:831661/1‑150 (MQ=255)
                                                                          ctGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATtaat                                                                          >  2:1536771/1‑150 (MQ=255)
                                                                            gCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATtaataa                                                                        >  2:778513/1‑150 (MQ=255)
                                                                            gCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATtaataa                                                                        <  2:20361/150‑1 (MQ=255)
                                                                            gCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAAAGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATtaataa                                                                        <  1:164842/150‑1 (MQ=255)
                                                                             cTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTaataat                                                                       <  2:164882/150‑1 (MQ=255)
                                                                               cccGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACCTACAACATAATCCCACCTTATTACTCAGACCCTTCTATTGATATGCATGAATAAtta                                                                     >  2:983134/1‑149 (MQ=255)
                                                                               cccGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTc                                                                     <  2:545004/150‑1 (MQ=255)
                                                                               cccGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGAGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTc                                                                     <  2:887572/150‑1 (MQ=255)
                                                                                ccGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCt                                                                    <  2:855450/150‑1 (MQ=255)
                                                                                    gcAGTCTAGCCAGGATGGAGCGCCTGCCGTACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGATCATTCAACATAATCCCACCTTATTACTCATACCCTTATATTGATAAGGATTAATAATACTTAAc                                                                >  1:1005099/1‑150 (MQ=255)
                                                                                     cAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAAcc                                                               <  2:1561368/150‑1 (MQ=255)
                                                                                      aGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAAccc                                                              <  1:1507120/150‑1 (MQ=255)
                                                                                       gTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTATATTGATATGGATTAATAATTCTTAACCCa                                                             >  1:1086509/1‑150 (MQ=255)
                                                                                         cTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCaaa                                                           >  2:1892010/1‑150 (MQ=255)
                                                                                          tAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCCCCTTATTACTCAGACCCTTCTATTGATATGGATTAATAATTCTTAACCCaaaa                                                          >  1:1381255/1‑150 (MQ=255)
                                                                                            gCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATg                                                        >  1:420005/1‑150 (MQ=255)
                                                                                             ccAGGATGGCGCGCCTGCCGCACCGCAGGACCACATTATCGACTGAACGCCGGATTTGACAAATCCGGAGATTTGAACATACAACATAATCCCACGTTATTACTCATAGCCTTCTATTGATATGGATTAATAAATCTTAACCCAAAATgg                                                       >  2:1232746/1‑150 (MQ=255)
                                                                                              cAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATggg                                                      >  2:490716/1‑150 (MQ=255)
                                                                                              cAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATggg                                                      >  2:1168589/1‑150 (MQ=255)
                                                                                               aGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGt                                                     <  2:931581/150‑1 (MQ=255)
                                                                                                    ggCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACt                                                <  2:1391731/150‑1 (MQ=255)
                                                                                                    ggCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACAAAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACt                                                <  1:339547/150‑1 (MQ=255)
                                                                                                     gcgcgcCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTc                                               >  1:901203/1‑150 (MQ=255)
                                                                                                          ccTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCctct                                          <  1:135090/150‑1 (MQ=255)
                                                                                                             gCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTAtt                                       >  2:1000307/1‑150 (MQ=255)
                                                                                                              ccGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATCGGTAGACTCCATCTAttt                                      >  1:643226/1‑149 (MQ=255)
                                                                                                                gAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATACTCACACCTTATTACTCATACCCATCTATTGAAATGCATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTCtt                                    >  1:198200/1‑150 (MQ=255)
                                                                                                                gAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATAGtt                                    >  2:394535/1‑150 (MQ=255)
                                                                                                                gAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGCGTAGACTCCCTCTATTGtt                                    >  2:1624702/1‑150 (MQ=255)
                                                                                                                  aCCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAg                                  <  2:1880470/150‑1 (MQ=255)
                                                                                                                  aCCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACACTTCTATTGATATGGATTAATAATTCTTAAACCAAAATGGGTAGACTCCCTCTATTTTTAg                                  >  1:1201840/1‑150 (MQ=255)
                                                                                                                     gCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATTGATTAATAATTCTTAACCAAAAATGGGTAGACTCCCTCTATTGTTAgcgc                               >  1:1433047/1‑150 (MQ=255)
                                                                                                                     gCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAgcgc                               >  1:1038829/1‑150 (MQ=255)
                                                                                                                      cAGTAACACATTATCGACTGAACGCCGTATTTGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTCCTCATACCCTTCTATTGATATGGATTAAAAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAgcgcg                              <  1:1036461/150‑1 (MQ=255)
                                                                                                                       agaaaCACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAgcgcgc                             <  2:1314143/147‑1 (MQ=255)
                                                                                                                       aGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAgcgcgc                             <  2:116250/150‑1 (MQ=255)
                                                                                                                       aGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAAAAAACAACATAATCCCACCTTATTACTCATACACTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAtcgcgc                             >  1:1542253/1‑150 (MQ=255)
                                                                                                                         tAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTa                           <  1:623711/150‑1 (MQ=255)
                                                                                                                            cacaTTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGCCTCGATCTATTGTTAGCGCGCTAAat                        >  2:1701821/1‑150 (MQ=255)
                                                                                                                            cacaTTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAat                        <  2:1548882/150‑1 (MQ=255)
                                                                                                                                ttATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTc                    <  2:528125/150‑1 (MQ=255)
                                                                                                                                ttATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTc                    <  2:955271/150‑1 (MQ=255)
                                                                                                                                 tATCGACTGACCGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCa                   <  1:182271/150‑1 (MQ=255)
                                                                                                                                 tATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCa                   <  2:1463524/150‑1 (MQ=255)
                                                                                                                                  aTCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCaa                  <  1:272010/150‑1 (MQ=255)
                                                                                                                                   tCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCaaa                 <  1:1624515/150‑2 (MQ=255)
                                                                                                                                    cGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAata                >  1:4419/1‑150 (MQ=255)
                                                                                                                                    cGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAata                <  1:1836913/150‑1 (MQ=255)
                                                                                                                                         gAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAATATATaa           >  2:1766240/1‑150 (MQ=255)
                                                                                                                                         aatcGCCGGATTTGTCATATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAATATATaa           >  2:1580787/4‑150 (MQ=255)
                                                                                                                                          aaCGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAATATATaaa          >  2:393156/1‑150 (MQ=255)
                                                                                                                                          aaCGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAATATATAAAc         <  2:183824/150‑1 (MQ=255)
                                                                                                                                            cGCCGGATTTGTCATATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAATATATAAACt        >  1:413608/1‑150 (MQ=255)
                                                                                                                                            cGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAATATATAAACt        >  2:431430/1‑150 (MQ=255)
                                                                                                                                            cGCCGGATATGACAAATCCGGAGATTTGAACATACAACATATTCGCACCTTATTACTCATACCCTTATATAGATATGGATTAATAATTCATAAACCAAAATGGGTAGACTCGCTCTATAGTTAGCGCGCTAAATATTCATTTTATAAACt        >  2:1284316/1‑150 (MQ=255)
                                                                                                                                               cGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCTACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAATATATAAACtttt     >  1:1099036/1‑150 (MQ=255)
                                                                                                                                               cGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAATATATAAACtttt     >  2:435217/1‑150 (MQ=255)
                                                                                                                                                ggATATGACAAATCCGGCGATTTGAACATACAACATAATCCCATCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAATATATAAACTTTta    <  2:1834288/150‑1 (MQ=255)
                                                                                                                                                ggATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAATATATAAACTTTta    <  1:113793/150‑1 (MQ=255)
                                                                                                                                                  ttatGACAATTCAGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAATATATAAACTTTtata  <  2:1895404/149‑1 (MQ=255)
                                                                                                                                                  atatGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAATATATAAACTTTtata  >  2:2009446/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTCCCAGGTCGTCGGCCATTGCGCTAATGATACGCATAAAATTCTCTATACCCGCACCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAATATATAAACTTTTATA  >  NC_000913/1657747‑1658042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: