Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,661,935 G→T 7.2% L460L (CTG→CTT)  ynfF → S‑ and N‑oxide reductase, A subunit, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,661,9350GT 7.2% 267.6 / 9.3 98L460L (CTG→CTT) ynfFS‑ and N‑oxide reductase, A subunit, periplasmic
Reads supporting (aligned to +/- strand):  ref base G (47/43);  new base T (4/4);  total (51/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01

Only 100 of 102 total aligned reads displayed.

GAAGGTAGCTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAA  >  NC_000913/1661786‑1662084
                                                                                                                                                     |                                                                                                                                                     
gAAGGTTGCTGGGATCTGGGTGTATAATTGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCTTCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTg                                                                                                                                                       <  2:1011676/150‑1 (MQ=255)
 aaGGTAGCTGGTATGTGGGGGTATAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTgg                                                                                                                                                      <  2:1642916/150‑1 (MQ=255)
 aaGGTAGCTGGGATCTGGGTGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTgg                                                                                                                                                      <  1:1364861/150‑1 (MQ=255)
     tAGCTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGt                                                                                                                                                  >  1:1449591/1‑150 (MQ=255)
       gCTGGGATCTGGGGGTAGAATGGTTCCCGCTGCTCGAGACTCCTGTGTCACCGCTGATTTCCGTCTTTACATGGACAGATGCGATCGACCATGGTACGGAAATGCCCGCGACCCGTGATGGTGGTCGTGGAAAAGAAAAACTGGATGTcc                                                                                                                                                >  1:327781/1‑150 (MQ=255)
        cTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAAATGCCATCGACCATGGTTCGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTccc                                                                                                                                               <  2:390585/150‑1 (MQ=255)
        cTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAAATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTccc                                                                                                                                               <  2:1415428/150‑1 (MQ=255)
               cTGGGGGTAGAATGGTTCCCGATGCTCGAGAATACTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAg                                                                                                                                        >  1:296095/1‑150 (MQ=255)
                tGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGt                                                                                                                                       >  2:209987/1‑150 (MQ=255)
                   gtgTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGtttt                                                                                                                                    <  2:1079544/148‑1 (MQ=255)
                   gggTAGAATGGTTCCCGATGCTCGAGATTCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGtttt                                                                                                                                    <  1:1980154/150‑1 (MQ=255)
                   gggTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGtttt                                                                                                                                    >  2:107069/1‑150 (MQ=255)
                   gggTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCGTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGAGACCCGTGATGGTGTTAGTGGATAAGATAAACTGGATGTCCCCATCAAGtttt                                                                                                                                    >  1:227419/1‑150 (MQ=255)
                     gTAGAATGGGTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTa                                                                                                                                  <  2:492361/150‑1 (MQ=255)
                        gAATGGTTCCCGATTCTCGAGAATCCTGTTAAAACGCATATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATgg                                                                                                                               <  2:1340643/150‑1 (MQ=255)
                        gAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATgg                                                                                                                               >  1:523846/1‑150 (MQ=255)
                        gAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATgg                                                                                                                               >  1:1081906/1‑150 (MQ=255)
                        gAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATgg                                                                                                                               >  1:1780143/1‑150 (MQ=255)
                         aaTGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGt                                                                                                                              <  2:850020/150‑1 (MQ=255)
                         aaTGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGt                                                                                                                              <  1:1993636/150‑1 (MQ=255)
                          aTGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTg                                                                                                                             >  2:352643/1‑150 (MQ=255)
                           ttgTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATTGTACGGAAATGTCCGCTACCCGTGATGGTTTTCGTGGAAATGAAAAACTGGATGTCCCCATCATTTTTTTATGGTGc                                                                                                                            <  2:1368486/148‑1 (MQ=255)
                           tGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTTGATGGGGACATCCAGtttttcttttcc                                                                                                                            <  1:1617800/150‑8 (MQ=255)
                           tGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGGCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTTGATGGGGACATCCAGtttttcttttcc                                                                                                                            <  1:1076177/150‑8 (MQ=255)
                               tCCCGATGCTCGAGAATCCTGATAAAACGCAGATTTCCTTCTTTACATGTACAGATGCCATCGACCATGGTACGGAAATGACCGCGACACGTGATGGTGTGCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACg                                                                                                                        <  2:296095/150‑1 (MQ=255)
                                  cGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCa                                                                                                                     >  1:665151/1‑150 (MQ=255)
                                   gATGCTCGAGAATCCTGTTAAACCGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAg                                                                                                                    <  2:756815/150‑1 (MQ=255)
                                     tGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTa                                                                                                                  >  1:876627/1‑150 (MQ=255)
                                     tGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTa                                                                                                                  >  1:586030/1‑150 (MQ=255)
                                         cGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCTCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAacac                                                                                                              >  1:54538/1‑150 (MQ=255)
                                          gagaATCCTGTTAAAACGCAGATTTCCGTCTTTCCATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAAcaca                                                                                                             <  1:1347742/150‑1 (MQ=255)
                                          gagaATCCTGTTAAAACGCAGATTTCCGTCTTTACATCGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGGTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAAcaca                                                                                                             <  2:347456/150‑1 (MQ=255)
                                             aaTCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTg                                                                                                          <  2:1912789/150‑1 (MQ=255)
                                                  tGTTAAAACGCAGAATTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGatcaa                                                                                                     <  1:1315231/150‑1 (MQ=255)
                                                    ttAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGCCCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTATTATGGTGCTACGCCAGTAACACATTGatcaatc                                                                                                   >  2:1861770/1‑150 (MQ=255)
                                                    ttAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGatcaatc                                                                                                   <  1:887539/150‑1 (MQ=255)
                                                          cGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATg                                                                                             >  1:1860230/1‑150 (MQ=255)
                                                          cGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATg                                                                                             >  1:524564/1‑150 (MQ=255)
                                                            cAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGc                                                                                           >  1:1135422/1‑150 (MQ=255)
                                                            cAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGc                                                                                           <  1:344151/150‑1 (MQ=255)
                                                              gATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGa                                                                                         >  2:1102575/1‑150 (MQ=255)
                                                               aTTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGAc                                                                                        >  1:1000581/1‑150 (MQ=255)
                                                                  accGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGAGATGGTGTTCGTGGAAAAGAAAACCTGCATGTCCCCAACAAGTATATATGGAGCTACGCCAGTCACACATTGATCCATCAACATGGCGCCatc                                                                                     >  2:289552/2‑150 (MQ=255)
                                                                    cGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACatcaa                                                                                   >  1:1468100/1‑150 (MQ=255)
                                                                      tCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACatcaatc                                                                                 <  1:1134030/150‑1 (MQ=255)
                                                                      tCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACatcaatc                                                                                 <  1:641160/150‑1 (MQ=255)
                                                                      tCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACatcaata                                                                                 >  1:938807/1‑149 (MQ=255)
                                                                      tCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCATGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACatcaatc                                                                                 >  1:1493368/1‑150 (MQ=255)
                                                                       cTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACatcaatca                                                                                <  1:2009767/150‑1 (MQ=255)
                                                                       cTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACatcaatca                                                                                >  1:1841931/1‑150 (MQ=255)
                                                                        tttACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATcac                                                                               >  1:1955594/1‑150 (MQ=255)
                                                                           aCATGGACAGATGCCATCGACCATGGTACGGAAATGACCTCGACCCTTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACAcc                                                                            <  1:1800133/150‑1 (MQ=255)
                                                                            cATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGAGGAAAAGAAAAACTGGATGTCACCATCAAGTTATTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACAccc                                                                           >  1:51980/1‑150 (MQ=255)
                                                                              tGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTATTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCCCACCCAt                                                                         >  2:1718501/1‑150 (MQ=255)
                                                                               ggACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATg                                                                        <  2:1421904/150‑1 (MQ=255)
                                                                               ggACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATg                                                                        >  2:1660471/1‑150 (MQ=255)
                                                                               ggACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATg                                                                        >  2:1528599/1‑150 (MQ=255)
                                                                                gACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGa                                                                       <  2:1465864/150‑1 (MQ=255)
                                                                                  cAGATGCCATCGACCATTGTACGGAAATGACCGCGACCCGTGATGGTGTGAGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTATTTATGGTGCTACGCCAGTATCACATTTATCACTCAACATGGCGACATCAATCACACCCATTAgg                                                                     <  2:965259/150‑1 (MQ=255)
                                                                                  cAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAgg                                                                     <  2:1188313/150‑1 (MQ=255)
                                                                                     aTGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACTCCAGTAACACATTCATCCATCACCATGGCGACATCAAACACACCCAGGCGGTGc                                                                  >  1:365696/1‑150 (MQ=255)
                                                                                      tGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCt                                                                 <  1:107069/150‑1 (MQ=255)
                                                                                              cccATGGTACTGAAATGACCGCTACCCGTGATGCGGTTCGTGGAAAAGAAAAACTGGATGTCGCCATCAAGTTTTTATGGTGCTACGCCAGTAAGACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATg                                                         <  2:564104/149‑1 (MQ=255)
                                                                                              aCCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGATGTGCTTCAGGATg                                                         <  1:438527/150‑1 (MQ=255)
                                                                                              aCCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATg                                                         >  2:1010475/1‑150 (MQ=255)
                                                                                               ccATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGa                                                        >  2:850899/1‑150 (MQ=255)
                                                                                               acATTGTACGGAAATGACCGCTACCCGTGATGGTTTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAG‑GTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGAc                                                       <  1:1234690/149‑1 (MQ=255)
                                                                                                cATGGTACGGAAATGACCGCGATCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGAc                                                       <  2:1750118/150‑1 (MQ=255)
                                                                                                       cGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCTAGt                                                >  1:1079149/1‑150 (MQ=255)
                                                                                                       cGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGt                                                >  1:1242465/1‑150 (MQ=255)
                                                                                                            aTGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCgag                                           <  2:665151/150‑1 (MQ=255)
                                                                                                             tgcccGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCgaga                                          >  2:15744/4‑150 (MQ=255)
                                                                                                             tGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCgaga                                          >  1:1756864/1‑150 (MQ=255)
                                                                                                               aCCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAgatg                                        >  2:953910/1‑150 (MQ=255)
                                                                                                                      cccGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGAttgttg                                 >  2:625317/1‑150 (MQ=255)
                                                                                                                       gcGTGATGGTGTTCGTTGAAAAGAAAACCTTGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTgg                                <  1:1393501/149‑1 (MQ=255)
                                                                                                                       ccGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTgg                                <  1:1471667/150‑1 (MQ=255)
                                                                                                                       ccGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTgg                                <  2:1955594/150‑1 (MQ=255)
                                                                                                                         gTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCa                              <  1:53130/150‑1 (MQ=255)
                                                                                                                            aTGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTg                           <  2:523846/150‑1 (MQ=255)
                                                                                                                             tGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGa                          <  1:127859/150‑1 (MQ=255)
                                                                                                                              ggTGTTCGTGGAAAAGAAAAACTTGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTTATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATTATTGTTGGCATTGAc                         <  1:1504187/150‑1 (MQ=255)
                                                                                                                              ggTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGTTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGAc                         <  1:728240/150‑1 (MQ=255)
                                                                                                                                   tCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACtt                    >  1:517410/1‑150 (MQ=255)
                                                                                                                                     gTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCGACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCa                  <  1:281952/150‑1 (MQ=255)
                                                                                                                                      tGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCAt                 <  2:1607000/150‑1 (MQ=255)
                                                                                                                                      tGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCAt                 <  2:703427/150‑1 (MQ=255)
                                                                                                                                       ggAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGAGCTTCAGGATGACAGCAAGAGCGAGATGATTGTTGGCATTGACCACTTCaag                >  2:1207233/1‑148 (MQ=255)
                                                                                                                                        gaaaagaaaaACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGa               >  1:490780/1‑150 (MQ=255)
                                                                                                                                        caccataaaaaCTTGATGGGGACATCCAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGa               >  1:1676050/7‑150 (MQ=255)
                                                                                                                                        caccataaaaaCTTGATGGGGACATCCAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGa               >  1:1715966/7‑150 (MQ=255)
                                                                                                                                        caccataaaaaCTTGATGGGGACATCCAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGa               >  1:1869319/7‑150 (MQ=255)
                                                                                                                                         accataaaaaCTTGATGGGGACATCCAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGAc              >  1:475069/6‑150 (MQ=255)
                                                                                                                                         aaaagaaaaACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGAc              >  2:1921643/1‑150 (MQ=255)
                                                                                                                                          aaagaaaaACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACg             <  2:54538/150‑1 (MQ=255)
                                                                                                                                            agaaaaACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGc           >  2:1094069/1‑150 (MQ=255)
                                                                                                                                                aaaCTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCg       <  1:1102575/150‑1 (MQ=255)
                                                                                                                                                  aCTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGc     <  2:1500322/150‑1 (MQ=255)
                                                                                                                                                  aCTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGc     >  1:726619/1‑150 (MQ=255)
                                                                                                                                                     ggATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTaa  >  2:828596/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GAAGGTAGCTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAA  >  NC_000913/1661786‑1662084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑