Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,661,942 C→G 7.1% P463A (CCC→GCC) ‡ ynfF → S‑ and N‑oxide reductase, A subunit, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,661,9420CG 7.1% 276.7 / 10.1 95P463A (CCC→GCC) ‡ynfFS‑ and N‑oxide reductase, A subunit, periplasmic
Reads supporting (aligned to +/- strand):  ref base C (45/42);  new base G (4/3);  total (50/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

Only 100 of 104 total aligned reads displayed.

GCTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGC  >  NC_000913/1661793‑1662091
                                                                                                                                                     |                                                                                                                                                     
gCTGGGATCTGGGGGTAGAATGGTTCCCGCTGCTCGAGACTCCTGTGTCACCGCTGATTTCCGTCTTTACATGGACAGATGCGATCGACCATGGTACGGAAATGCCCGCGACCCGTGATGGTGGTCGTGGAAAAGAAAAACTGGATGTcc                                                                                                                                                       >  1:327781/1‑150 (MQ=255)
 cTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAAATGCCATCGACCATGGTTCGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTccc                                                                                                                                                      <  2:390585/150‑1 (MQ=255)
 cTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAAATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTccc                                                                                                                                                      <  2:1415428/150‑1 (MQ=255)
        cTGGGGGTAGAATGGTTCCCGATGCTCGAGAATACTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAg                                                                                                                                               >  1:296095/1‑150 (MQ=255)
         tGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGt                                                                                                                                              >  2:209987/1‑150 (MQ=255)
            gtgTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGtttt                                                                                                                                           <  2:1079544/148‑1 (MQ=255)
            gggTAGAATGGTTCCCGATGCTCGAGATTCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGtttt                                                                                                                                           <  1:1980154/150‑1 (MQ=255)
            gggTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGtttt                                                                                                                                           >  2:107069/1‑150 (MQ=255)
            gggTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCGTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGAGACCCGTGATGGTGTTAGTGGATAAGATAAACTGGATGTCCCCATCAAGtttt                                                                                                                                           >  1:227419/1‑150 (MQ=255)
              gTAGAATGGGTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTa                                                                                                                                         <  2:492361/150‑1 (MQ=255)
                 gAATGGTTCCCGATTCTCGAGAATCCTGTTAAAACGCATATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATgg                                                                                                                                      <  2:1340643/150‑1 (MQ=255)
                 gAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATgg                                                                                                                                      >  1:1081906/1‑150 (MQ=255)
                 gAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATgg                                                                                                                                      >  1:523846/1‑150 (MQ=255)
                 gAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATgg                                                                                                                                      >  1:1780143/1‑150 (MQ=255)
                  aaTGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGt                                                                                                                                     <  1:1993636/150‑1 (MQ=255)
                  aaTGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGt                                                                                                                                     <  2:850020/150‑1 (MQ=255)
                   aTGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTg                                                                                                                                    >  2:352643/1‑150 (MQ=255)
                    ttgTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATTGTACGGAAATGTCCGCTACCCGTGATGGTTTTCGTGGAAATGAAAAACTGGATGTCCCCATCATTTTTTTATGGTGc                                                                                                                                   <  2:1368486/148‑1 (MQ=255)
                    tGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTTGATGGGGACATCCAGtttttcttttcc                                                                                                                                   <  1:1617800/150‑8 (MQ=255)
                    tGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGGCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTTGATGGGGACATCCAGtttttcttttcc                                                                                                                                   <  1:1076177/150‑8 (MQ=255)
                        tCCCGATGCTCGAGAATCCTGATAAAACGCAGATTTCCTTCTTTACATGTACAGATGCCATCGACCATGGTACGGAAATGACCGCGACACGTGATGGTGTGCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACg                                                                                                                               <  2:296095/150‑1 (MQ=255)
                           cGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCa                                                                                                                            >  1:665151/1‑150 (MQ=255)
                            gATGCTCGAGAATCCTGTTAAACCGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAg                                                                                                                           <  2:756815/150‑1 (MQ=255)
                              tGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTa                                                                                                                         >  1:586030/1‑150 (MQ=255)
                              tGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTa                                                                                                                         >  1:876627/1‑150 (MQ=255)
                                  cGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCTCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAacac                                                                                                                     >  1:54538/1‑150 (MQ=255)
                                   gagaATCCTGTTAAAACGCAGATTTCCGTCTTTCCATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAAcaca                                                                                                                    <  1:1347742/150‑1 (MQ=255)
                                   gagaATCCTGTTAAAACGCAGATTTCCGTCTTTACATCGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGGTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAAcaca                                                                                                                    <  2:347456/150‑1 (MQ=255)
                                      aaTCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTg                                                                                                                 <  2:1912789/150‑1 (MQ=255)
                                           tGTTAAAACGCAGAATTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGatcaa                                                                                                            <  1:1315231/150‑1 (MQ=255)
                                             ttAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGCCCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTATTATGGTGCTACGCCAGTAACACATTGatcaatc                                                                                                          >  2:1861770/1‑150 (MQ=255)
                                             ttAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGatcaatc                                                                                                          <  1:887539/150‑1 (MQ=255)
                                                   cGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATg                                                                                                    >  1:524564/1‑150 (MQ=255)
                                                     cAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGc                                                                                                  >  1:1135422/1‑150 (MQ=255)
                                                     cAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGc                                                                                                  <  1:344151/150‑1 (MQ=255)
                                                       gATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGa                                                                                                >  2:1102575/1‑150 (MQ=255)
                                                        aTTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGAc                                                                                               >  1:1000581/1‑150 (MQ=255)
                                                           accGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGAGATGGTGTTCGTGGAAAAGAAAACCTGCATGTCCCCAACAAGTATATATGGAGCTACGCCAGTCACACATTGATCCATCAACATGGCGCCatc                                                                                            >  2:289552/2‑150 (MQ=255)
                                                             cGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACatcaa                                                                                          >  1:1468100/1‑150 (MQ=255)
                                                               tCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACatcaatc                                                                                        <  1:641160/150‑1 (MQ=255)
                                                               tCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACatcaatc                                                                                        <  1:1134030/150‑1 (MQ=255)
                                                               tCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACatcaata                                                                                        >  1:938807/1‑149 (MQ=255)
                                                               tCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCATGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACatcaatc                                                                                        >  1:1493368/1‑150 (MQ=255)
                                                                cTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACatcaatca                                                                                       <  1:2009767/150‑1 (MQ=255)
                                                                cTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACatcaatca                                                                                       >  1:1841931/1‑150 (MQ=255)
                                                                 tttACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATcac                                                                                      >  1:1955594/1‑150 (MQ=255)
                                                                    aCATGGACAGATGCCATCGACCATGGTACGGAAATGACCTCGACCCTTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACAcc                                                                                   <  1:1800133/150‑1 (MQ=255)
                                                                     cATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGAGGAAAAGAAAAACTGGATGTCACCATCAAGTTATTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACAccc                                                                                  >  1:51980/1‑150 (MQ=255)
                                                                        ggACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATg                                                                               <  2:1421904/150‑1 (MQ=255)
                                                                        ggACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATg                                                                               >  2:1528599/1‑150 (MQ=255)
                                                                        ggACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATg                                                                               >  2:1660471/1‑150 (MQ=255)
                                                                         gACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGa                                                                              <  2:1465864/150‑1 (MQ=255)
                                                                           cAGATGCCATCGACCATTGTACGGAAATGACCGCGACCCGTGATGGTGTGAGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTATTTATGGTGCTACGCCAGTATCACATTTATCACTCAACATGGCGACATCAATCACACCCATTAgg                                                                            <  2:965259/150‑1 (MQ=255)
                                                                           cAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAgg                                                                            <  2:1188313/150‑1 (MQ=255)
                                                                              aTGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACTCCAGTAACACATTCATCCATCACCATGGCGACATCAAACACACCCAGGCGGTGc                                                                         >  1:365696/1‑150 (MQ=255)
                                                                               tGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCt                                                                        <  1:107069/150‑1 (MQ=255)
                                                                                       cccATGGTACTGAAATGACCGCTACCCGTGATGCGGTTCGTGGAAAAGAAAAACTGGATGTCGCCATCAAGTTTTTATGGTGCTACGCCAGTAAGACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATg                                                                <  2:564104/149‑1 (MQ=255)
                                                                                       aCCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGATGTGCTTCAGGATg                                                                <  1:438527/150‑1 (MQ=255)
                                                                                       aCCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATg                                                                >  2:1010475/1‑150 (MQ=255)
                                                                                        ccATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGa                                                               >  2:850899/1‑150 (MQ=255)
                                                                                        acATTGTACGGAAATGACCGCTACCCGTGATGGTTTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGGTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGAc                                                              <  1:1234690/149‑1 (MQ=255)
                                                                                         cATGGTACGGAAATGACCGCGATCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGAc                                                              <  2:1750118/150‑1 (MQ=255)
                                                                                                cGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCTAGt                                                       >  1:1079149/1‑150 (MQ=255)
                                                                                                cGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGt                                                       >  1:1242465/1‑150 (MQ=255)
                                                                                                     aTGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCgag                                                  <  2:665151/150‑1 (MQ=255)
                                                                                                      tgcccGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCgaga                                                 >  2:15744/4‑150 (MQ=255)
                                                                                                      tGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCgaga                                                 >  1:1756864/1‑150 (MQ=255)
                                                                                                        aCCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAgatg                                               >  2:953910/1‑150 (MQ=255)
                                                                                                               cccGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGAttgttg                                        >  2:625317/1‑150 (MQ=255)
                                                                                                                gcGTGATGGTGTTCGTTGAAAAGAAAACCTTGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTgg                                       <  1:1393501/149‑1 (MQ=255)
                                                                                                                ccGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTgg                                       <  1:1471667/150‑1 (MQ=255)
                                                                                                                ccGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTgg                                       <  2:1955594/150‑1 (MQ=255)
                                                                                                                  gTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCa                                     <  1:53130/150‑1 (MQ=255)
                                                                                                                     aTGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTg                                  <  2:523846/150‑1 (MQ=255)
                                                                                                                      tGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGa                                 <  1:127859/150‑1 (MQ=255)
                                                                                                                       ggTGTTCGTGGAAAAGAAAAACTTGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTTATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATTATTGTTGGCATTGAc                                <  1:1504187/150‑1 (MQ=255)
                                                                                                                       ggTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGTTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGAc                                <  1:728240/150‑1 (MQ=255)
                                                                                                                            tCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACtt                           >  1:517410/1‑150 (MQ=255)
                                                                                                                              gTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCa                         >  1:657517/1‑150 (MQ=255)
                                                                                                                              gTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCGACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCa                         <  1:281952/150‑1 (MQ=255)
                                                                                                                               tGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCAt                        <  2:703427/150‑1 (MQ=255)
                                                                                                                               tGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCAt                        <  2:1607000/150‑1 (MQ=255)
                                                                                                                                ggAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGAGCTTCAGGATGACAGCAAGAGCGAGATGATTGTTGGCATTGACCACTTCaag                       >  2:1207233/1‑148 (MQ=255)
                                                                                                                                 gaaaagaaaaACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGa                      >  1:490780/1‑150 (MQ=255)
                                                                                                                                 caccataaaaaCTTGATGGGGACATCCAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGa                      >  1:1676050/7‑150 (MQ=255)
                                                                                                                                 caccataaaaaCTTGATGGGGACATCCAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGa                      >  1:1715966/7‑150 (MQ=255)
                                                                                                                                 caccataaaaaCTTGATGGGGACATCCAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGa                      >  1:1869319/7‑150 (MQ=255)
                                                                                                                                  accataaaaaCTTGATGGGGACATCCAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGAc                     >  1:475069/6‑150 (MQ=255)
                                                                                                                                  aaaagaaaaACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGAc                     >  2:1921643/1‑150 (MQ=255)
                                                                                                                                   aaagaaaaACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACg                    <  2:54538/150‑1 (MQ=255)
                                                                                                                                     agaaaaACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGc                  >  2:1094069/1‑150 (MQ=255)
                                                                                                                                         aaaCTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCg              <  1:1102575/150‑1 (MQ=255)
                                                                                                                                           aCTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGc            >  1:726619/1‑150 (MQ=255)
                                                                                                                                           aCTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGc            <  2:1500322/150‑1 (MQ=255)
                                                                                                                                              ggATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTaa         >  2:828596/1‑150 (MQ=255)
                                                                                                                                                    ccccATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTGAGTATTg   >  2:173031/1‑150 (MQ=255)
                                                                                                                                                    ccccATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTg   >  2:1167394/1‑150 (MQ=255)
                                                                                                                                                    ccccATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTg   >  1:456148/1‑150 (MQ=255)
                                                                                                                                                     cccATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGc  <  2:1583012/150‑1 (MQ=255)
                                                                                                                                                     cccAACAAGTATTTATGGTGCTACTCCAGTAACAGATTGATCAATCAACATGGCGAGATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGTGATGATTGGTGGCATTGACCACTTCTTGACGGCCTCGGCTAAGTATTGc  <  2:158670/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGC  >  NC_000913/1661793‑1662091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: