Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,684,019 C→T 8.0% R380W (CGG→TGG)  rstB → sensory histidine kinase of RstAB two‑component system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,684,0190CT 8.0% 369.6 / 13.3 124R380W (CGG→TGG) rstBsensory histidine kinase of RstAB two‑component system
Reads supporting (aligned to +/- strand):  ref base C (60/54);  new base T (7/3);  total (67/57)
Fisher's exact test for biased strand distribution p-value = 3.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 125 total aligned reads displayed.

CGCCCTGCGGTACTGCCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAA  >  NC_000913/1683871‑1684168
                                                                                                                                                    |                                                                                                                                                     
cGCCCTGCGGTACTGCCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCg                                                                                                                                                      <  2:18220/150‑1 (MQ=255)
   ccTGCGGTACTGCCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGa                                                                                                                                                   <  1:757316/150‑1 (MQ=255)
        ggTACTGCCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCt                                                                                                                                              <  1:1023555/150‑1 (MQ=255)
         gTACTGCCATTCAACGGGTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCGGCTGGGATCgagg                                                                                                                                             <  1:1439411/150‑4 (MQ=255)
          tACTGCCATTCCACGGTTGACACCAGCCTTCTACTGGCGGGGAATAGAGCGACATTAATTTTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCGGCTGGGATCgaggc                                                                                                                                            <  1:1241954/150‑5 (MQ=255)
            cTGCCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGAAGGCCCAGGGATTGCCCCAGAACACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAAc                                                                                                                                          >  1:273528/1‑150 (MQ=255)
             tGCCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAAcc                                                                                                                                         >  2:838520/1‑150 (MQ=255)
             tGCCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAAcc                                                                                                                                         >  1:1451354/1‑150 (MQ=255)
             tGCCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGCATCGCTCAAcc                                                                                                                                         >  1:1613455/1‑150 (MQ=255)
              gCCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACcg                                                                                                                                        >  1:988605/1‑150 (MQ=255)
                 aTTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACcggcg                                                                                                                                     <  2:1608736/150‑1 (MQ=255)
                      aCGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAAATGTTGAGGATGAGGGCCCAGGGATTGCCCCAGAAAACCGCGAACATAGCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGc                                                                                                                                <  1:37552/150‑1 (MQ=255)
                       cGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCCGCCGGGATCGCTCAACCGGCGGCTGCg                                                                                                                               >  1:1990077/1‑150 (MQ=255)
                        ggTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACGGGCGGCTGCgg                                                                                                                              <  2:738780/150‑1 (MQ=255)
                           tGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCgggct                                                                                                                           >  2:425645/1‑150 (MQ=255)
                            gAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCgggctg                                                                                                                          <  2:525882/150‑1 (MQ=255)
                               aCCAGCCTGCTACTGTCGGGGAAAAGAGCGACATTAATTGTTGAGGAGGATGGCCCAGGGATTGCCCCAGAAACCCGCGAACATCTCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCgggctgggg                                                                                                                       <  1:1079771/150‑1 (MQ=255)
                                 cAGCCTGCTCCTGGCGGGTAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCTGGATCGCTCAACCGGCGGCTGCgggctggggct                                                                                                                     <  1:1600966/150‑1 (MQ=255)
                                   gCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGggctggggctgg                                                                                                                   <  2:489173/150‑1 (MQ=255)
                                    ccTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCCGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGc                                                                                                                  >  1:1831411/1‑150 (MQ=255)
                                    ccTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGc                                                                                                                  <  1:323672/150‑1 (MQ=255)
                                     cTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCa                                                                                                                 >  2:1733771/1‑150 (MQ=255)
                                      tGCTACGGTCGGGGAATAGAGCTACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCaa                                                                                                                <  1:1807933/150‑1 (MQ=255)
                                       gCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAAt                                                                                                               <  2:1454129/150‑1 (MQ=255)
                                         tACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTg                                                                                                             >  1:2018596/1‑150 (MQ=255)
                                         tACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTg                                                                                                             >  1:779174/1‑150 (MQ=255)
                                           cTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCCGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTc                                                                                                           >  2:1849172/1‑150 (MQ=255)
                                              tCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCAc                                                                                                        <  1:1956206/150‑1 (MQ=255)
                                              tCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCAc                                                                                                        <  1:885151/150‑1 (MQ=255)
                                              tCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCAc                                                                                                        <  1:425645/150‑1 (MQ=255)
                                              tCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCGCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCAc                                                                                                        <  2:1287789/150‑1 (MQ=255)
                                                       agagCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCa                                                                                               <  1:871547/150‑1 (MQ=255)
                                                       agagCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTATGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCa                                                                                               >  2:1765924/1‑150 (MQ=255)
                                                        gagCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGTTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCAc                                                                                              >  1:1632774/1‑150 (MQ=255)
                                                          gCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATTGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTg                                                                                            >  1:1590662/1‑150 (MQ=255)
                                                          ccGACATTAATTGGTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTg                                                                                            <  2:714951/149‑1 (MQ=255)
                                                              cATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGATCCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCaa                                                                                        <  2:225435/150‑1 (MQ=255)
                                                                ttAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATg                                                                                      >  1:1356609/1‑150 (MQ=255)
                                                                  aaTTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACATTTAGTTCGCCTCGATCCCACCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATggg                                                                                    >  2:1056307/1‑150 (MQ=255)
                                                                   aTTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGTCAATTGTCCACTCTATAGCACTGGCAATGGGc                                                                                   <  2:1613455/150‑1 (MQ=255)
                                                                   aTTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGc                                                                                   <  1:685185/150‑1 (MQ=255)
                                                                   aTTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGc                                                                                   <  2:1454142/150‑1 (MQ=255)
                                                                    ttgttgAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCg                                                                                  >  2:768046/1‑150 (MQ=255)
                                                                      gttgAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGt                                                                                >  1:1715406/1‑150 (MQ=255)
                                                                          aGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACgg                                                                            <  1:1573019/150‑1 (MQ=255)
                                                                           ggATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCACCCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGt                                                                           >  2:1442111/1‑150 (MQ=255)
                                                                              tgatgGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTaa                                                                        >  1:645121/1‑150 (MQ=255)
                                                                              tgatgGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTaa                                                                        >  2:1584313/1‑150 (MQ=255)
                                                                                 tgGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGGACCTTTTGTTCGCCTCGATCACAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCCGTCTATAGCACTGGCAATGGGCGGTACGGTTATTtg                                                                     >  2:979406/1‑150 (MQ=255)
                                                                                 tgGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATtg                                                                     >  1:555961/1‑150 (MQ=255)
                                                                                  ggCCCAGGGATTGCACCAGAAAACCGCGAACATATATTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCTGGCTGGGGCTGGAAATTGTCCACTCTATTGCACTGGCAATGGGCGGTACGGTTAATtgt                                                                    >  1:1255615/1‑150 (MQ=255)
                                                                                   gCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATtgtg                                                                   <  1:838520/150‑1 (MQ=255)
                                                                                    cccAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGATCGCCTCGCTACCAGCCGGGATCGCTCACCCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCCCTCTATAGCTCTGGCAATGGGCGGGTCGGTTAATTGTGa                                                                  >  1:108394/1‑150 (MQ=255)
                                                                                            ttGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGAGCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCg                                                          <  2:845372/150‑1 (MQ=255)
                                                                                                cccAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACt                                                      >  1:704463/1‑150 (MQ=255)
                                                                                                 ccAGAAAACCGCGAACATATCTTTGCCCCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTg                                                     >  1:803148/1‑150 (MQ=255)
                                                                                                            cgAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGTCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGTCACCAGCGAACTGGGTGTTGCCCg                                          >  2:275062/1‑150 (MQ=255)
                                                                                                            cgAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCg                                          <  2:1134225/150‑1 (MQ=255)
                                                                                                             gAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGc                                         <  2:273528/150‑1 (MQ=255)
                                                                                                                cATATCTTTGAACCTTTTGTGCGCCTCGATCCCAGCCGGGATCGCTCCACCGGCGGCTGCGGGCGGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTtc                                      <  1:1816547/150‑1 (MQ=255)
                                                                                                                  tatCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTtctc                                    >  2:1925217/1‑150 (MQ=255)
                                                                                                                    tCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGt                                  >  2:1718291/1‑150 (MQ=255)
                                                                                                                     cTTTGACCCGGTAGTGCGCCTCGCCACCCGCCCGGATCGCTCAACCGGCGGCTGCGGCCGGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGtt                                 <  1:1246948/150‑1 (MQ=255)
                                                                                                                     cTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGtt                                 >  2:1136028/1‑150 (MQ=255)
                                                                                                                     cTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGtt                                 <  2:2018596/150‑1 (MQ=255)
                                                                                                                       ttGAACCTTTTGTTCGCCTCGATCCCCGCCGGGATCGCTCAACCGGCGGCGGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTa                               <  2:751887/150‑1 (MQ=255)
                                                                                                                       ttGAACCTTTTGTTCGCCGCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTa                               <  1:1857621/150‑1 (MQ=255)
                                                                                                                         gAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGc                             >  2:517210/1‑150 (MQ=255)
                                                                                                                         gAAACTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCAATATATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAgg                             >  2:1028142/1‑149 (MQ=255)
                                                                                                                          aaCCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGGCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCt                            <  1:914152/150‑1 (MQ=255)
                                                                                                                             cTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGc                         >  1:542666/1‑150 (MQ=255)
                                                                                                                             cTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGc                         <  2:847348/150‑1 (MQ=255)
                                                                                                                              ttttGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGcc                        >  1:673035/1‑150 (MQ=255)
                                                                                                                              ttttGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGcc                        >  2:1875669/1‑150 (MQ=255)
                                                                                                                                 tGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACTCCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGtt                     >  1:32798/1‑150 (MQ=255)
                                                                                                                                 tGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGTCCGCTTCTCGTTTAGCTGGCCGtt                     >  1:1377370/1‑150 (MQ=255)
                                                                                                                                 tGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGTCCGCTTCTCGTTTAGCTGGCCGtt                     >  1:863082/1‑150 (MQ=255)
                                                                                                                                  gTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGGTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCATCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTa                    >  2:962279/1‑150 (MQ=255)
                                                                                                                                  gTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTa                    <  2:1779233/150‑1 (MQ=255)
                                                                                                                                  gTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGAAATGGGCGGTACGGTTAATTGTGACACCAGCGATCTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTa                    >  2:1450035/1‑150 (MQ=255)
                                                                                                                                   ttCGGCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTATTTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTAt                   <  1:1733771/150‑1 (MQ=255)
                                                                                                                                   ttCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTAt                   >  1:11991/1‑150 (MQ=255)
                                                                                                                                   ttCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGGTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTAt                   >  2:720655/1‑150 (MQ=255)
                                                                                                                                   ggttgagcGATCCCGGCTGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTAt                   >  2:431263/8‑150 (MQ=255)
                                                                                                                                      tgagcGATCCCGGCTGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTATTTAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGCTTAGCTGGCCGTTATGGc                >  2:1051876/5‑150 (MQ=255)
                                                                                                                                      tgagcGATCCCGGCTGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCAGGCAATTGTCCACTCTATAGCACTGGCAATCGGCGCTACGGTTAATTGTGACACAAGCGAACTGGGTGGTGACAGCTTCTCGTTTAGCTGGAAGTTATGGc                >  1:1038375/5‑150 (MQ=255)
                                                                                                                                      gCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGc                <  2:1383487/150‑1 (MQ=255)
                                                                                                                                       gagcGATCCCGGCTGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGGAATGGGCGGTACGGTTACTTGTGACACCAGCGAAGTGGGTGGTGCCCGCTTCTCGTTTAGCGGGCCGTTATGGCa               >  1:201295/4‑150 (MQ=255)
                                                                                                                                       gagcGATCCCGGCTGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCa               >  1:483831/4‑150 (MQ=255)
                                                                                                                                        cTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCAt              >  2:1856806/1‑150 (MQ=255)
                                                                                                                                         tCGATCCCAGCCCGGATCGCTCAACCGGGGGCTGCGGGCTGGTGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATa             <  2:763670/150‑1 (MQ=255)
                                                                                                                                          cGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTTTGACACCAGCGATCTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATaa            >  2:965393/1‑150 (MQ=255)
                                                                                                                                           gATCCCAGCCGGGATCGCTCAACCGGCGGTTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAAc           <  2:1632774/150‑1 (MQ=255)
                                                                                                                                           gATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGTGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAAc           >  2:747901/1‑150 (MQ=255)
                                                                                                                                           gATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAAc           >  2:753080/1‑150 (MQ=255)
                                                                                                                                           gATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAAc           >  1:1289766/1‑150 (MQ=255)
                                                                                                                                                 cgCCGGGATCGCTCAACCGGCGGCGGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCg     <  2:901472/149‑1 (MQ=255)
                                                                                                                                                 aGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCg     <  2:1831411/150‑1 (MQ=255)
                                                                                                                                                  gCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGc    >  2:591697/1‑150 (MQ=255)
                                                                                                                                                    cGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCTGTACGGTTAATTGTGACACCAGCGAACTGGGTGTTGCCCGCTTCTAGTTTATCTTGCCGTTATGGCATAACATCCCGCaa  >  2:98093/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGCCCTGCGGTACTGCCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAA  >  NC_000913/1683871‑1684168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: