Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 236 C→G 6.6% T16R (ACA→AGA) ‡ thrL → thr operon leader peptide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132360CG 6.6% 372.2 / 13.8 119T16R (ACA→AGA) ‡thrLthr operon leader peptide
Reads supporting (aligned to +/- strand):  ref base C (72/39);  new base G (5/3);  total (77/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

Only 100 of 121 total aligned reads displayed.

GCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACG  >  NC_000913/88‑383
                                                                                                                                                    |                                                                                                                                                    
gCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACcaca                                                                                                                                                     <  1:188834/150‑1 (MQ=255)
  cGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGACATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAgg                                                                                                                                                   <  1:1681077/150‑1 (MQ=255)
      aGTAAATTAAAATTTCATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAAc                                                                                                                                               >  2:629657/1‑150 (MQ=255)
        tAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACgg                                                                                                                                             <  2:1320402/150‑1 (MQ=255)
        tAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACACATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGCTAACgg                                                                                                                                             >  2:1729160/1‑150 (MQ=255)
           aTTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCTGTGGTAATGGTga                                                                                                                                          <  1:1028031/150‑2 (MQ=255)
               aaaTTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATTGCGCACAGACAGATAAAAATTACAGAGTACACAACAACCATGTAACGCATTAGCACCACCATTACCACCACCATCACCGTTACCTGTGGTAATGGTgatggt                                                                                                                                      <  2:104628/150‑6 (MQ=255)
               aaaTTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCGTTACCTGTGGTAATGGTgatggt                                                                                                                                      <  2:1421077/150‑6 (MQ=255)
                gtatttAGTGCCCTAAGTCAATAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCt                                                                                                                                     >  1:1472146/4‑150 (MQ=255)
                 aTTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTg                                                                                                                                    >  1:632373/1‑150 (MQ=255)
                    ttATTGACTTAGGTCACTAAATACTTTATCCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGAcg                                                                                                                                 <  2:1369790/150‑1 (MQ=255)
                      aTTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGAcgcg                                                                                                                               >  1:34095/1‑150 (MQ=255)
                          aCTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGCCGCGTACa                                                                                                                           >  1:228716/1‑150 (MQ=255)
                          aCTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACa                                                                                                                           <  2:905601/150‑1 (MQ=255)
                          aCTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACa                                                                                                                           <  1:928798/150‑1 (MQ=255)
                                gTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAac                                                                                                                     <  2:1173387/150‑1 (MQ=255)
                                gTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAac                                                                                                                     <  1:1108579/150‑1 (MQ=255)
                                 tCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAaca                                                                                                                    >  2:712170/1‑150 (MQ=255)
                                  cACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAacac                                                                                                                   >  2:503349/1‑150 (MQ=255)
                                  cACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAacac                                                                                                                   >  2:1437508/1‑150 (MQ=255)
                                      aaataaatTAACCAATATAGGCATAGCGCAAAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGAAATAGCACCACCAATACCACCAACATCACCATAACCAGAGGTAACGGTGCGGGCTGCCGGGTACTCGAACCACAGaa                                                                                                               >  1:976637/1‑150 (MQ=255)
                                       aaTACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGaaa                                                                                                              <  2:929758/150‑1 (MQ=255)
                                           cTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACAACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAg                                                                                                          >  1:1296911/1‑150 (MQ=255)
                                             ttAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGcc                                                                                                        >  1:1336704/1‑150 (MQ=255)
                                                aCCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGc                                                                                                     >  2:1373119/1‑150 (MQ=255)
                                                aCCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGc                                                                                                     >  1:641536/1‑150 (MQ=255)
                                                aCCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGc                                                                                                     >  1:389417/1‑150 (MQ=255)
                                                aCCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGAATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGTAAAAAGCCCGc                                                                                                     >  2:524535/1‑150 (MQ=255)
                                                       taGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACAACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGAc                                                                                              >  2:276055/1‑150 (MQ=255)
                                                        aGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACa                                                                                             >  2:1758409/1‑150 (MQ=255)
                                                         ggCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGAGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCCACTGACac                                                                                            >  1:1506743/1‑149 (MQ=255)
                                                          gCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCAGTAGCACCCCCATTACCACCACCATCACCATTACCACAGGTAACGGAGCGGGCTGCCGCGTACAGGACACACAGAAAAAAGCCCGCCccagccagg                                                                                          >  1:394141/1‑149 (MQ=255)
                                                              aGCGCACAGACTGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTggcg                                                                                      >  2:911939/1‑147 (MQ=255)
                                                               gcgcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCggg                                                                                     <  2:1209034/150‑1 (MQ=255)
                                                                cgcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTCCCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAACAGCCCGCACCTGACAGTGCGGGc                                                                                    <  1:546117/150‑1 (MQ=255)
                                                                cgcACAGACAGATAAAAATTACAGAGTACAAACCATCCATGAAACGCATTAGCACAACCATTACCACCACCATCACCATTACCACAGTTAACGGTTCTGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGc                                                                                    <  1:1933132/150‑1 (MQ=255)
                                                                 gcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACACGTAACGGTGCGGGCTGACGCGTACAGAAAACACAGAAAAAAGCCCGCCCCTGACCGTGCggtcg                                                                                   >  1:1240467/1‑147 (MQ=255)
                                                                 gcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATAAGAACGACCATTACCACCACCATCACCATTAGCACAGGTAACGGTGCGGGCTTACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCt                                                                                   >  2:680629/1‑150 (MQ=255)
                                                                    cagacagaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCtttt                                                                                >  2:1982060/1‑150 (MQ=255)
                                                                      gacagaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCtttttt                                                                              >  2:1477091/1‑150 (MQ=255)
                                                                        cagaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGTTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCtttttttt                                                                            <  2:40443/150‑1 (MQ=255)
                                                                          gaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCCCCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACTGTCAGGTGCGGGCttttttctgt                                                                          <  1:1300974/150‑5 (MQ=38)
                                                                           aTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCg                                                                         >  2:1819911/1‑150 (MQ=255)
                                                                             ataaaTTACAGAGTACACTACATCCATGAAACGCATTAGCACCACCATTACCACCTCCATCTCCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAACCACAGAAAACAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGAc                                                                       <  2:375420/148‑1 (MQ=255)
                                                                              aaaaTTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGAcc                                                                      >  1:1639548/1‑150 (MQ=255)
                                                                               aaaTTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCTGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCa                                                                     >  1:242959/1‑150 (MQ=255)
                                                                                aaTTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCaa                                                                    <  1:672966/150‑1 (MQ=255)
                                                                                aaTTACAGAGTACACAACATCCAGGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACCGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCaa                                                                    <  1:201592/150‑1 (MQ=255)
                                                                                 aTTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCCCCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCaaa                                                                   <  1:939270/150‑1 (MQ=255)
                                                                                  ttACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAg                                                                  >  2:1170421/1‑150 (MQ=255)
                                                                                    aCAGAGTACACAACATCCATGAAACGCATTAGAACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCAGACGCGTACAGGAAACACAGAAAAAAGCCCGAACCTGACAGTGCGGGCTTTTTTGTTCGACCAAAGGt                                                                >  2:1214648/1‑150 (MQ=255)
                                                                                     cAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTa                                                               <  2:534827/150‑1 (MQ=255)
                                                                                      agagTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGCCCAAAGGTaa                                                              >  2:1708209/1‑150 (MQ=255)
                                                                                      agagTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTaa                                                              >  1:811434/1‑150 (MQ=255)
                                                                                       cagaaaacaacaTCCATGAAACGCATTACCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAAc                                                             <  1:1752826/144‑1 (MQ=255)
                                                                                           acacAACATCCATGAAACGCATTCGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAgg                                                         <  1:2011585/150‑1 (MQ=255)
                                                                                           acacAACATCCATGAAACGCATTCGCACCACCATTACCACCACCATCACCATAACCACAGGTAACGGTGCGGGCGGACGCGTACAGGAAACACAGAAAAACGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAgg                                                         <  1:510222/150‑1 (MQ=255)
                                                                                           acacAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAgg                                                         <  1:1554539/150‑1 (MQ=255)
                                                                                            cacaACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGTGCTTTTTTTTTCGACCAAAGGTAACGACGt                                                        >  1:631730/1‑150 (MQ=255)
                                                                                            cacaACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGt                                                        <  1:1437508/150‑1 (MQ=255)
                                                                                            cacaACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTAGACCAAAGGTAACGAGGt                                                        >  2:859199/1‑150 (MQ=255)
                                                                                            cacaACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTAGACCAAAGGTAACGAGGt                                                        >  1:1055564/1‑150 (MQ=255)
                                                                                                 caTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTaacaa                                                   >  2:399103/1‑150 (MQ=255)
                                                                                                  aTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTaacaac                                                  >  2:642865/1‑150 (MQ=255)
                                                                                                    ccATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACACCCa                                                >  1:578252/1‑150 (MQ=255)
                                                                                                    ccATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCa                                                >  1:698304/1‑150 (MQ=255)
                                                                                                         aaaCGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGa                                           >  2:862019/1‑150 (MQ=255)
                                                                                                          aaCGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAg                                          >  1:1344337/1‑150 (MQ=255)
                                                                                                           aCGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAgt                                         <  1:232044/150‑1 (MQ=255)
                                                                                                            cGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAgtg                                        <  2:1146205/150‑1 (MQ=255)
                                                                                                                   gCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCCCCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGt                                 >  2:423148/1‑150 (MQ=255)
                                                                                                                    caccacCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACCACCATGCGAGTGTTGAAGtt                                >  2:1680777/1‑150 (MQ=255)
                                                                                                                     accaccATTACCACCACCATAACCATTACCACAGGTAACGGTGCGGGATGACGCGTACAGGCAACACATAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTc                               <  1:1823926/150‑1 (MQ=255)
                                                                                                                       caccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcgg                             >  2:1623205/1‑150 (MQ=255)
                                                                                                                       caccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcgg                             >  1:1204439/1‑150 (MQ=255)
                                                                                                                        accaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcggc                            <  2:269081/150‑1 (MQ=255)
                                                                                                                        accaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcggc                            >  2:1561131/1‑150 (MQ=255)
                                                                                                                             tACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTAc                       <  2:632373/150‑1 (MQ=255)
                                                                                                                              accaccaccATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACa                      >  1:1538310/1‑150 (MQ=255)
                                                                                                                               ccaccaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGaa                                   >  2:495257/1‑136 (MQ=255)
                                                                                                                               ccaccaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACAt                     >  1:386919/1‑150 (MQ=255)
                                                                                                                               ccaccaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAATGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACAt                     >  1:792870/1‑150 (MQ=255)
                                                                                                                                 accaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGt                         <  2:1877408/144‑1 (MQ=255)
                                                                                                                                 accaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGt                         >  1:1877408/1‑144 (MQ=255)
                                                                                                                                    accatcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGCCCAAAGGTAACGAGGTACCAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTg                >  2:982533/1‑150 (MQ=255)
                                                                                                                                    accatcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTg                <  2:1199853/150‑1 (MQ=255)
                                                                                                                                     cccgcaccgtTACCTGTGGTAATGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTgg               >  2:729459/5‑150 (MQ=255)
                                                                                                                                     ccatcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTTACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTgg               >  1:402029/1‑150 (MQ=255)
                                                                                                                                     ccatcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTgg               >  1:518034/1‑150 (MQ=255)
                                                                                                                                      ccgcaccgtTACCTGTGGTAATGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGc              >  1:1304390/4‑150 (MQ=255)
                                                                                                                                      catcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGGGTTGAAGTTCGGCGGTACATCAGTGGc              >  2:515966/1‑150 (MQ=255)
                                                                                                                                       cgcaccgtTACCTGTGGTAATGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCa             >  1:1484349/3‑150 (MQ=255)
                                                                                                                                       atcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCa             >  1:1701131/1‑150 (MQ=255)
                                                                                                                                       atcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCa             <  2:1790669/150‑1 (MQ=255)
                                                                                                                                          accatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGGAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAAt          >  2:2012623/1‑150 (MQ=255)
                                                                                                                                          accatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCCACCAAAGGTAACGAGGTAACCACCATTCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAAt          >  1:55633/1‑150 (MQ=255)
                                                                                                                                          accatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAACCCCGCACCTGACAGTGCGGGCTTTTTTTTTCCACCAAAGGTAACGAGGTAACAACCATGCGAGTGTAGAAGTTAGGCGGTACATCAGTGGCAAAt          >  2:640706/1‑150 (MQ=255)
                                                                                                                                                aCCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGaa    >  2:880980/1‑150 (MQ=255)
                                                                                                                                                 ccACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAAc   >  2:1039319/1‑150 (MQ=255)
                                                                                                                                                  cacaGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAACAACGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGTGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACg  <  2:1557100/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCCGTGAGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACG  >  NC_000913/88‑383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: