Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,693,417 G→C 5.9% L283V (CTG→GTG) ‡ uidB ← glucuronide transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,693,4170GC 5.9% 430.9 / 12.6 146L283V (CTG→GTG) ‡uidBglucuronide transporter
Reads supporting (aligned to +/- strand):  ref base G (64/73);  new base C (2/7);  total (66/80)
Fisher's exact test for biased strand distribution p-value = 1.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01

Only 100 of 148 total aligned reads displayed.

CCAATTGAAGCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGCACACACAGCGCACCGATGCACA  >  NC_000913/1693269‑1693566
                                                                                                                                                    |                                                                                                                                                     
ccAATTGAAGCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGc                                                                                                                                                      <  1:385887/150‑1 (MQ=255)
ccAATTGAAGCGAAGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGc                                                                                                                                                      <  2:1105357/150‑1 (MQ=255)
  aaTTGAAGCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCgg                                                                                                                                                    <  1:1534379/150‑1 (MQ=255)
    ttGAAGCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTg                                                                                                                                                  <  1:1887059/150‑1 (MQ=255)
     tGAAGCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGc                                                                                                                                                 >  1:927977/1‑150 (MQ=255)
      gAAGCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGcc                                                                                                                                                <  1:1593512/150‑1 (MQ=255)
       aaGCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCg                                                                                                                                               <  2:1670378/150‑1 (MQ=255)
         gCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCGCTGGTGCCggg                                                                                                                                             <  1:593502/150‑3 (MQ=255)
         gCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCGCTGGTGCCggg                                                                                                                                             <  1:1898008/150‑3 (MQ=255)
         gCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCGCTGGTGCCggg                                                                                                                                             <  2:1776694/150‑3 (MQ=255)
         gCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCGCGGGTGCCggg                                                                                                                                             <  1:1890261/150‑3 (MQ=255)
         gCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGAt                                                                                                                                             <  2:1207634/150‑1 (MQ=255)
         gCGATGGCCAACGCAACCAACGCCACCGGCAGGGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCACTCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGAt                                                                                                                                             <  2:1737687/150‑1 (MQ=255)
          cGATGGCCAAGGCAACCTACGCCACCGGCAGTGACCAGACGGAAACCCAGTTGAACAGCTCATAACCGCCGGTTCCCACCAAAGCGCCAATCAGGAAGGTATTCTTTTTAGCGAACCTCGCGACCATCCCCCGCACCAGCGGTGCCGATg                                                                                                                                            <  2:1171667/150‑1 (MQ=255)
          cGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCGCTGGTGCCgggg                                                                                                                                            <  1:1976801/150‑4 (MQ=255)
            aTGGCCAACGCAACCAACTCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCTGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTATACCGATCCTCGCGACCATCCCCGGCACCCGCGGTGCCGATGcc                                                                                                                                          <  1:318797/150‑1 (MQ=255)
            aTGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGcc                                                                                                                                          <  2:1666079/150‑1 (MQ=255)
              ggCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAACCCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCcac                                                                                                                                        >  2:860858/1‑150 (MQ=255)
              ggCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGCCCATCCCCGGCACCAGCGGTGCCGCTGCcac                                                                                                                                        >  1:1498132/1‑150 (MQ=255)
                  aaCGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGCTCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTa                                                                                                                                    >  1:154724/1‑150 (MQ=255)
                   aCGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAATGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTac                                                                                                                                   <  1:1420705/150‑1 (MQ=255)
                     gCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTacca                                                                                                                                 >  2:1868086/1‑150 (MQ=255)
                          caacGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAg                                                                                                                            <  2:1543959/150‑1 (MQ=255)
                          caacGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCACATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAg                                                                                                                            <  2:1952533/150‑1 (MQ=255)
                          caacGCCACCGGCAGTGACCAGACGCAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAg                                                                                                                            <  2:630083/150‑1 (MQ=255)
                           aacGCCACCGGAAGTGACCATACGCAAACCCAGAAGAACAGCTGATAACCGCAGGTTCCCAGAAAAGCGCAAATCAGTAAGGTATTCTTTTTACCTATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAgg                                                                                                                           <  2:1668199/150‑1 (MQ=255)
                            acGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCACCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGt                                                                                                                          <  1:1353446/150‑1 (MQ=255)
                              gCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGttt                                                                                                                        >  2:572407/1‑150 (MQ=255)
                              gCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGttt                                                                                                                        >  2:751480/1‑150 (MQ=255)
                              gCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGttt                                                                                                                        >  2:687920/1‑150 (MQ=255)
                              gCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGttt                                                                                                                        >  1:1923119/1‑150 (MQ=255)
                               ccACCGGCAGTGACCAGACGGAAACCCAGCAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCGTCAGTACCAACCAGGtttt                                                                                                                       >  2:1027831/1‑150 (MQ=255)
                               ccACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGtttt                                                                                                                       <  1:1418678/150‑1 (MQ=255)
                               ccACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGtttt                                                                                                                       <  2:1207225/150‑1 (MQ=255)
                                cACCGGCAGTGCCCAGAAGGAAACCCACCAGACCAGCCCATAACACCAGGTTCCCTCCAAAGCCCCAATCAGCACGGTATTCTTTTTACCGATCGGCGGTCCCTTCCCCCGCACCCCCGGGGCCGAAGCCACAGTACCAACCTCGTTTTg                                                                                                                      <  2:837063/150‑1 (MQ=255)
                                cACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGAAACCCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCCGCGGTGCCGATGCCACAGTACCAACCAGGTTTTg                                                                                                                      <  2:639029/150‑1 (MQ=255)
                                 aCCGGCAGTGACCAGACGGAAACCCAGAATAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGTAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGc                                                                                                                     <  1:911568/150‑1 (MQ=255)
                                  ccGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCa                                                                                                                    <  2:1268316/150‑1 (MQ=255)
                                  ccGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCAAGCAAAGCGCAAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCa                                                                                                                    <  2:1267032/150‑1 (MQ=255)
                                      cAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTTTTCTTTATACCGATCCGCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAg                                                                                                                <  1:139333/150‑1 (MQ=255)
                                        gTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTa                                                                                                              >  1:460724/1‑150 (MQ=255)
                                          gACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTAcc                                                                                                            <  2:808534/150‑1 (MQ=255)
                                             cAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGATGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGc                                                                                                         <  2:502684/150‑1 (MQ=255)
                                             cAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGc                                                                                                         >  1:670317/1‑150 (MQ=255)
                                             cAGACGGAAACCCAGAAGAACAGCAGATAACCGCACGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGCCCAACCCCGGCACCAGCGGTGCCGATGCCCCAGTACCAACCAGGTTTTGCCCCAGTCCCAGc                                                                                                         >  1:978549/1‑150 (MQ=255)
                                                 cGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAg                                                                                                     >  1:1164375/1‑150 (MQ=255)
                                                   gAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTg                                                                                                   <  2:1300128/150‑1 (MQ=255)
                                                     aaCCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCCGCACAGTGaa                                                                                                 >  1:782880/1‑150 (MQ=255)
                                                      aCCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAAc                                                                                                <  1:1787983/150‑1 (MQ=255)
                                                         cAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACAAGCACAGTGAACAGc                                                                                             >  1:567219/1‑150 (MQ=255)
                                                           taagaaCAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGccc                                                                                           <  1:692693/149‑1 (MQ=255)
                                                           gaagaaCAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTAACGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCAACAGTACCAGCACAGTGAACAGccc                                                                                           <  2:1098030/150‑1 (MQ=255)
                                                                   gcagATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCAt                                                                                   <  1:622050/150‑1 (MQ=255)
                                                                    cagATAACCGCAGGTTCCCAGCAAAGCGCCAAGCAGGAAGGTATTCTTTTTACCGATCCTCGCGCCCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCAtt                                                                                  <  2:252999/150‑1 (MQ=255)
                                                                     agATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCACGCGACCATCCCCGGCACCCGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCGCCAGTACCAGCACAGTGAACAGCCCGGTATCAgtt                                                                                 >  2:630402/1‑150 (MQ=255)
                                                                        tAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAac                                                                              <  2:724326/150‑1 (MQ=255)
                                                                        aaaCCGCAGGTTCCAAGCAAAGCGCCAATCTGTATGTTTTTCTTTTTACGGATCCTCGCCACCATCCCCGTCACCAGGGGTGCGGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAac                                                                              <  1:1760082/149‑1 (MQ=255)
                                                                         aaCCGCAGGTTCCCAGCAAAGCGCCAATCAGGAATGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAaca                                                                             <  1:863644/150‑1 (MQ=255)
                                                                          aCCGCAGGTTCCCAGCAAAGCGCCAATCAGGATGGTATTCTTTTTACCGATCCTCGCGACAATCCCAGGCACCAGCGGTGCCGATGCCACAGTACAAACCAGGTTTTGCACCAGTACCAGCACAGTGAACCGCCCGGTATCATTTAacac                                                                            >  2:962101/1‑150 (MQ=255)
                                                                          aCCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAacac                                                                            <  2:971823/150‑1 (MQ=255)
                                                                          aCCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAacaa                                                                            >  2:1355537/1‑149 (MQ=255)
                                                                           ccGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAAcaca                                                                           >  1:561592/1‑150 (MQ=255)
                                                                            cGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTGGCGACCATCGACGGGAGCAGCGGTGCCGATGCCACATTACCAACCATTTTTTGCACCATTACCAGCACAGTGAACAGCCCGGTATCATTTAACACAt                                                                          <  1:1131818/150‑1 (MQ=255)
                                                                            cGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACAt                                                                          <  1:1733793/150‑1 (MQ=255)
                                                                                 gTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGCTCCTCGCGACCATCCCCGCCACCAGCGGTGCCGCTGCCACAGTACCAACCAGGTATTGCACCAGTACCAGCACAGAGAACAGCCCGGTATCATTTAACACATAgcgc                                                                     >  2:1574867/1‑150 (MQ=255)
                                                                                  ttCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTATACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCa                                                                    <  2:896221/150‑1 (MQ=255)
                                                                                   tCCCAGCAAAGCTCCATTCAGGAAGGTATTCTTTTTACAGATCCTCGCGACCATCCCCGGCACCAGCGTTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCAc                                                                   <  2:1226401/150‑1 (MQ=255)
                                                                                   tCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGAATCATTTAACACATAGCGCAc                                                                   >  2:369532/1‑150 (MQ=255)
                                                                                        gCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGa                                                              <  2:949337/150‑1 (MQ=255)
                                                                                          aaaGAGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATGCGCGGCACCTGCGGTGCCTATGACACAGTACGAACCTGGTTTTGCTCCAGTAACAGAACAGTGAACTGCCCGGTCTGTTTTAACACATAGCGCACTTAGatc                                                            <  2:1360340/150‑3 (MQ=255)
                                                                                           aaGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGaaca                                                           >  1:1654849/1‑150 (MQ=255)
                                                                                            aGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGACCAGCCCGGTATCATTTAACACATAGCGCACGTAGaacaa                                                          >  2:504259/1‑150 (MQ=255)
                                                                                             gcgcCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTGTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGaccaac                                                         >  1:975856/1‑150 (MQ=255)
                                                                                              cgcCAATCAGGAAGGTATTCTTTTTACCGATCCACGCGACCATCCCCGGAACCAGCGGTGACGATGCCACAGTACCAACCAGGTTTTGAACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAacg                                                        <  2:691887/150‑1 (MQ=255)
                                                                                                ccAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGATCAacgac                                                      >  1:498099/1‑150 (MQ=255)
                                                                                                ccAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAacgac                                                      >  2:865943/1‑150 (MQ=255)
                                                                                                 cAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCGACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAacgacg                                                     <  1:1782772/150‑1 (MQ=255)
                                                                                                    tCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAgg                                                  <  1:992468/150‑1 (MQ=255)
                                                                                                      aGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGgcg                                                >  2:438789/1‑150 (MQ=255)
                                                                                                         aaGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGACACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTg                                             >  2:672872/1‑150 (MQ=255)
                                                                                                                 cTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCCCCAGTACCAGCACAGTGCACCGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCaaa                                     >  2:342727/1‑150 (MQ=255)
                                                                                                                 cTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCaaa                                     <  1:1993326/150‑1 (MQ=255)
                                                                                                                       aCCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGCACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGa                               >  1:1122477/1‑150 (MQ=255)
                                                                                                                       aCCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAAACAGGTTTTGCACCAGTACCAGCCCAGTGAACAGCCCGGTATCATATAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGa                               <  1:1282993/150‑1 (MQ=255)
                                                                                                                          gATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACACCGACGAGGCGCTGACCGCAAAGGTCGAAAt                            >  2:1993520/1‑150 (MQ=255)
                                                                                                                          gATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGGGAACAGCCCGGTATCATTTAACACATAGCGCACGTCGAACATAGACGAGGCGCTGACCGCAATGGTCGaagt                            >  2:342344/1‑148 (MQ=255)
                                                                                                                           aTCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATc                           <  2:1662653/150‑1 (MQ=255)
                                                                                                                             ccTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAg                         <  2:1840063/150‑1 (MQ=255)
                                                                                                                                cgcgACCATCCCCGGCACCAGAGGTGCCAATGCCACAGTACCAACCAGGTTTTGAACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGAAGAACAACGACGAAGAGCTGCCCGCAAAGGTCGAAATCAGcac                      >  1:1281595/1‑150 (MQ=255)
                                                                                                                                 gcgACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGGTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGcaca                     <  2:1515470/150‑1 (MQ=255)
                                                                                                                                     ccATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACCGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGcacacacc                 >  1:196095/1‑149 (MQ=255)
                                                                                                                                       aTCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGCACACACAgc               >  1:1522176/1‑150 (MQ=255)
                                                                                                                                          cccGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACCGCCCGGTATCATTTAACACATAGCGCACGGTGAACAACGCAGCGGCGCTGACCGCAAAGGTCGAAATCAGCAGACACAGAGCa            >  2:1257784/1‑150 (MQ=255)
                                                                                                                                          cccGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGCACACACAGCGCa            <  2:61674/150‑1 (MQ=255)
                                                                                                                                          cccGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGCACACACAGCGCa            >  2:1376187/1‑150 (MQ=255)
                                                                                                                                          atcGGCACCGCTGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGCACACACAgcgcc            >  2:1989397/3‑149 (MQ=255)
                                                                                                                                                aCCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGCACACACCGCGCACCGATg      >  1:659628/1‑150 (MQ=255)
                                                                                                                                                aCCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGCACACACAGCGCACCGagg      >  2:1515241/1‑148 (MQ=255)
                                                                                                                                                  cAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGCACACACAGCGCACCGATGca    <  2:433228/150‑1 (MQ=255)
                                                                                                                                                  cAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGCACACACAGCGCACCGATGca    <  1:718455/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CCAATTGAAGCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGCACACACAGCGCACCGATGCACA  >  NC_000913/1693269‑1693566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑