Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,707,029 A→G 7.2% V37V (GTA→GTG) ‡ rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,707,0290AG 7.2% 310.0 / 12.2 116V37V (GTA→GTG) ‡rsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base A (41/62);  new base G (4/4);  total (49/67)
Fisher's exact test for biased strand distribution p-value = 7.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.52e-02

Only 100 of 123 total aligned reads displayed.

TGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTCGGG  >  NC_000913/1706880‑1707178
                                                                                                                                                     |                                                                                                                                                     
tGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTa                                                                                                                                                       >  2:1505164/1‑150 (MQ=255)
tGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTa                                                                                                                                                       >  2:1606947/1‑150 (MQ=255)
tGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCGCTGCATTCAGAAAAAATAAAATCTGGGATATCAACGGCGGCATCCATCCACCGGAGATGAAAAACCAGTCCAACGGTACACCCCTGCGCCAGGTa                                                                                                                                                       >  1:221814/1‑150 (MQ=255)
 gAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTAc                                                                                                                                                      <  1:896073/150‑1 (MQ=255)
    cacCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTAcccc                                                                                                                                                   <  2:145055/150‑1 (MQ=255)
    cacCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTAcccc                                                                                                                                                   <  2:661452/150‑1 (MQ=255)
    cacCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTAcccc                                                                                                                                                   >  2:47051/1‑150 (MQ=255)
       cATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTgg                                                                                                                                                >  2:2011807/1‑150 (MQ=255)
       cATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCACGTACCCCTgg                                                                                                                                                >  2:668733/1‑150 (MQ=255)
        aTTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGgc                                                                                                                                               <  1:552785/150‑1 (MQ=255)
           cccGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAAAGGCTGCATCCATCCACCTGAGATGAAAACCAAGTCCAACGGTACACCCCTGCGCCAGGTAACCCTGGCGCa                                                                                                                                            >  1:1294166/1‑150 (MQ=255)
            ccGTGCGTATCCTTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAg                                                                                                                                           <  1:62791/150‑1 (MQ=255)
             cGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGTGATTTCAACGGCGGCATCCATCCACCGGAGAGGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGc                                                                                                                                          <  1:289715/150‑1 (MQ=255)
             cGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGCTTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGc                                                                                                                                          <  2:1833994/150‑1 (MQ=255)
             cGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGc                                                                                                                                          <  1:1476731/150‑1 (MQ=255)
             cGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGGGTACCTGGCGCAgg                                                                                                                                          <  1:487490/150‑2 (MQ=255)
              gTGCGTATCCTTCCCCTGGAACACCATGCTTAAGTTTTTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATACATCCACCGGAGATGAACACCCAGTCCAACGGTACACCCCTGCGCCAGGGGTACCTGGCGCAggg                                                                                                                                         <  2:1262014/150‑3 (MQ=255)
              gTGCGTATCATTCCCGTGGAACACCATGCTTACGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCg                                                                                                                                         <  1:1119824/150‑1 (MQ=255)
              gTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCg                                                                                                                                         <  2:1655005/150‑1 (MQ=255)
                  gTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGtttt                                                                                                                                     <  1:1735115/150‑1 (MQ=255)
                  gTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGtttt                                                                                                                                     >  2:659791/1‑150 (MQ=255)
                   tATCATTCCCGTGGAAAACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCGGGGATTTCAACGGCGGCATCCATCCCCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCTGGCGCAGCGTTTTg                                                                                                                                    <  2:1489088/150‑1 (MQ=255)
                    aTCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGt                                                                                                                                   >  2:1844835/1‑150 (MQ=255)
                    aTCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGt                                                                                                                                   <  1:1097554/150‑1 (MQ=255)
                    aTCATTCCCGTGGAACACCATGCTTAAGTTAATCTCTGCATTCCGAAAAAATAAAATCTGGTATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGt                                                                                                                                   <  2:1243041/150‑1 (MQ=255)
                     tCATTCCCGTGGAACAACATGCTTAAGTTATTCTCTGCATTCATAAAAAATAAAAGCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGGCAGGTACCCCTGGCGCAGCGTTTTGtt                                                                                                                                  <  1:472564/150‑1 (MQ=255)
                      cATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTa                                                                                                                                 <  2:1972501/150‑1 (MQ=255)
                       aTTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTAt                                                                                                                                >  2:432793/1‑150 (MQ=255)
                         tCCCGTGGTACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAAGGGCGGCTTCCATCCACCGGAGATGAAAACCCAGTCCAACGGAACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTATTGTTATTc                                                                                                                              <  2:588547/150‑1 (MQ=255)
                         tCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTc                                                                                                                              <  2:1034418/150‑1 (MQ=255)
                         tCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTc                                                                                                                              <  1:1754579/150‑1 (MQ=255)
                          cccGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTcc                                                                                                                             <  2:1905874/150‑1 (MQ=255)
                           ccGTGGAACACCATTCTTAAGTTTTTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCTGCATCCATCCACCGGAGATGAAATCCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCa                                                                                                                            <  1:923031/150‑1 (MQ=255)
                           ccGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCa                                                                                                                            <  1:923034/150‑1 (MQ=255)
                            cGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCAc                                                                                                                           <  1:887581/150‑1 (MQ=255)
                              tGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTg                                                                                                                         <  2:1534726/150‑1 (MQ=255)
                                gAACACCATGCTGAAGTTATTCTCTGCATTCAGAAAATATAAAATCTGGGATTTCACCGGCGTCATCACTCCCCCGGAGATGAAAGCCCAGTCCAACGTTACACCCCTGCGCACGGGACCCCTGGCGCCGCGGTTTGGTATTccccgggt                                                                                                                       >  2:865300/1‑144 (MQ=255)
                                  acacCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAAc                                                                                                                     <  1:365/150‑1 (MQ=255)
                                   cacCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACa                                                                                                                    >  1:97871/1‑150 (MQ=255)
                                    acCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGTATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAg                                                                                                                   >  1:36896/1‑150 (MQ=255)
                                    acCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTCCAACAGGCGCCGCGTGATCTTATTCCACTGCAAAAg                                                                                                                   >  1:1335785/1‑150 (MQ=255)
                                      cATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCa                                                                                                                 <  1:1415784/150‑1 (MQ=255)
                                           ttAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTg                                                                                                            >  2:1959493/1‑150 (MQ=255)
                                            tAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATGCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTgg                                                                                                           <  1:1826802/150‑1 (MQ=255)
                                            tAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTgg                                                                                                           >  2:722963/1‑150 (MQ=255)
                                              aGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATACATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGgcg                                                                                                         <  2:180542/150‑1 (MQ=255)
                                               gTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGgcgc                                                                                                        <  1:1877513/150‑1 (MQ=255)
                                               gTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGgcgc                                                                                                        <  2:795633/150‑1 (MQ=255)
                                                     ctctGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTCCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAgg                                                                                                  >  1:1345374/1‑150 (MQ=255)
                                                     ctctGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGTAgg                                                                                                  >  1:1857739/1‑150 (MQ=255)
                                                               aGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGc                                                                                        <  1:985151/150‑1 (MQ=255)
                                                                gAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCg                                                                                       >  2:436130/1‑150 (MQ=255)
                                                                 aaaaaaTAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCAAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCTTATTGGCGCTGAAGGTGTGTTGTGCGt                                                                                      >  1:1339966/1‑150 (MQ=255)
                                                                      ataaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCg                                                                                 <  2:1764545/150‑1 (MQ=255)
                                                                          aatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAACCCCAGTCCAACGGTACACACCTGCGCCAGGTACCCCAGGCGCAGCGTGTTGGTATTCAACTGAAAAAGCATATTGGCGCGGATGGTGATTTGTGCGGTAGCGTCgg                                                                             >  1:968947/1‑150 (MQ=255)
                                                                          aatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGGTCCCCTGGGGCAGCGGTTTGTTTTTACACGGAAACACGAAATTTGCGCTGAAGGGGAGTTGTGCGTTAGCGTCgg                                                                             >  1:1470914/1‑150 (MQ=255)
                                                                           atCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACACCTGCGCCAGGTACCCCTGGAGCAGCGTTTTGTTATTCCCCTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGc                                                                            >  1:1425962/1‑150 (MQ=255)
                                                                             cTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACCGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGa                                                                          >  2:773834/1‑150 (MQ=255)
                                                                               gtgATTTCAACGTCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCTTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATa                                                                        <  2:1294166/148‑1 (MQ=255)
                                                                                   tttCAACGGCGTCATGCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCTTATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGt                                                                    <  2:779533/150‑1 (MQ=255)
                                                                                   tttCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGt                                                                    <  1:1797974/150‑1 (MQ=255)
                                                                                     tCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTAt                                                                  >  2:1362413/1‑150 (MQ=255)
                                                                                      cAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTTCCCCTGGCGCAGCGGTTTGTTATTCCAATAAAACCACATATTGGCGCGGAAGGTGAGTTGTGCGTTTGCGTCGGCGATTAAGGAtt                                                                 >  2:523547/1‑150 (MQ=255)
                                                                                       aaCGGCGGCATCCATCCACCGGAGATGAAACCCCAGTCCAACGGTAGACCCGTGCGCCAGGTTCCCCTGGCGCAGCGGTTTGTTATTCCTCTGAAACAGCATTTTGGCGGTGATGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTg                                                                <  2:1492385/150‑1 (MQ=255)
                                                                                       aaCGGCGCCATCCTTCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGGGACTTGTGCGTTAGCGTCGGCGATAAAGTATTg                                                                <  2:1059250/150‑1 (MQ=255)
                                                                                            cggcATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGCAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGGGTCGGCGATAAAGTATTGCGCgg                                                           >  1:1867687/1‑150 (MQ=255)
                                                                                            cggcATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCgg                                                           <  1:1959493/150‑1 (MQ=255)
                                                                                             ggcATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGc                                                          <  1:1061313/150‑1 (MQ=255)
                                                                                              gcATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGcc                                                         <  1:1505164/150‑1 (MQ=255)
                                                                                                   catccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTTGGCGATAAAGTATTGCGCGGCCAGCCg                                                    >  2:1123620/1‑150 (MQ=255)
                                                                                                     tccaCCGGAGATGAATACCCAGTCCAACGTTACACCCATGCGCCATGTACCCCTGGCGCAGCGGTTTGTTATTCCAGTGAAACAGCATATTGGCGCGGAAGGTGAGGTGTGCGTTAGCGTCGGCTATAAAGTATTGCGCGGCCAGCCGCt                                                  <  1:152398/150‑1 (MQ=255)
                                                                                                     tccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCt                                                  <  1:395727/150‑1 (MQ=255)
                                                                                                     tccaCCGGAGATGAAAACCCAGTCCAACGGAACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCt                                                  <  2:1622925/150‑1 (MQ=255)
                                                                                                      ccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTTTTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCtt                                                 >  2:1959277/1‑150 (MQ=255)
                                                                                                           ggAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCg                                            <  1:1356218/150‑1 (MQ=255)
                                                                                                           ggAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGGGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCg                                            <  1:131856/150‑1 (MQ=255)
                                                                                                             tgaTGAAAACCCAGTCCAAGGGTACACCCCTGCGCCAGGTACCCCTGGCTAAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTAGCCGTg                                          <  2:559178/149‑1 (MQ=255)
                                                                                                             agaTGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTg                                          <  2:1734810/150‑1 (MQ=255)
                                                                                                                tGAAACCCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTTAAACCAACTATTGGCGGTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAATTATTGCGCGGCCAGCCGCTTACCAGTGGTc                                       >  1:1254688/1‑150 (MQ=255)
                                                                                                                tGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCCCTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTc                                       >  1:237546/1‑150 (MQ=255)
                                                                                                                tGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTc                                       >  1:1371863/1‑150 (MQ=255)
                                                                                                                    aaCCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCgg                                   >  2:1158369/1‑150 (MQ=255)
                                                                                                                            ccAACGGTACACCCCTGCGCCAGGTACCCCTGGCGAAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAAtgc                           <  2:1482671/150‑1 (MQ=255)
                                                                                                                             cAACGGTACACCCCTGCGCCAGGTACCCCGGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGGATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAAtgcg                          >  2:1191833/1‑149 (MQ=255)
                                                                                                                              aaCGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAAtgctg                         <  1:516030/150‑1 (MQ=255)
                                                                                                                                cGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGcc                       <  1:1133399/150‑1 (MQ=255)
                                                                                                                                    acacCCCTGCGCCAGGTACCCCGGGCGCAGCGGTTTGTTATTCCACTGCAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGtt                   <  1:1408872/150‑1 (MQ=255)
                                                                                                                                     cacTCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTc                  <  2:858457/150‑1 (MQ=255)
                                                                                                                                     cacCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTc                  <  1:1656051/150‑1 (MQ=255)
                                                                                                                                        cccTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGACACAGCATATAGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCAcg               <  1:275539/150‑1 (MQ=255)
                                                                                                                                         gcTGCGCCAGGGGTACCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGGTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAACATGCTGCCTGTTCAcgc              >  1:1282876/2‑150 (MQ=255)
                                                                                                                                         gcTGCGCCAGGGGTACCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCAcgc              >  2:593937/2‑150 (MQ=255)
                                                                                                                                         gcTGCGCCAGGGGTACCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCAcgc              >  1:1267775/2‑150 (MQ=255)
                                                                                                                                         gcTGCGCCAGGGGTACCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCAcgc              >  1:884718/2‑150 (MQ=255)
                                                                                                                                          cTGCGCCAGGTACCCCTGGCGCAGGGGTTTGTTATTCGACTGAAAGAGCATATTGGCGCTGAAGGGGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCAcgcg             <  1:450722/150‑1 (MQ=255)
                                                                                                                                           tGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACgcgc            <  1:484811/150‑1 (MQ=255)
                                                                                                                                           tGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACgcgc            <  1:1006776/150‑1 (MQ=255)
                                                                                                                                                  ggTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTc     <  1:630488/150‑1 (MQ=255)
                                                                                                                                                     aCCCCTGGCGGAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGCTGAGTTGAGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTCggg  <  1:316743/150‑1 (MQ=255)
                                                                                                                                                     aCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTCggg  <  1:668733/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTCGGG  >  NC_000913/1706880‑1707178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: