Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,707,030 C→T 6.9% P38S (CCC→TCC) ‡ rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,707,0300CT 6.9% 357.1 / 10.6 116P38S (CCC→TCC) ‡rsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base C (43/64);  new base T (4/4);  total (48/68)
Fisher's exact test for biased strand distribution p-value = 7.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.28e-01

Only 100 of 120 total aligned reads displayed.

GAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTCGGG  >  NC_000913/1706881‑1707178
                                                                                                                                                     |                                                                                                                                                    
gAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTAc                                                                                                                                                      <  1:896073/150‑1 (MQ=255)
   cacCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTAcccc                                                                                                                                                   <  2:661452/150‑1 (MQ=255)
   cacCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTAcccc                                                                                                                                                   <  2:145055/150‑1 (MQ=255)
   cacCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTAcccc                                                                                                                                                   >  2:47051/1‑150 (MQ=255)
      cATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTgg                                                                                                                                                >  2:2011807/1‑150 (MQ=255)
      cATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCACGTACCCCTgg                                                                                                                                                >  2:668733/1‑150 (MQ=255)
          cccGTGCGTATGATTCCCGTGGAACACCAAGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGGGTACCTGGCGCa                                                                                                                                            <  2:165855/150‑1 (MQ=255)
          cccGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAAAGGCTGCATCCATCCACCTGAGATGAAAACCAAGTCCAACGGTACACCCCTGCGCCAGGTAACCCTGGCGCa                                                                                                                                            >  1:1294166/1‑150 (MQ=255)
           ccGTGCGTATCCTTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAg                                                                                                                                           <  1:62791/150‑1 (MQ=255)
            cGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGTGATTTCAACGGCGGCATCCATCCACCGGAGAGGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGc                                                                                                                                          <  1:289715/150‑1 (MQ=255)
            cGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGCTTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGc                                                                                                                                          <  2:1833994/150‑1 (MQ=255)
            cGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGGGTACCTGGCGCAgg                                                                                                                                          <  2:779161/150‑2 (MQ=255)
            cGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGGGTACCTGGCGCAgg                                                                                                                                          <  1:487490/150‑2 (MQ=255)
             gTGCGTATCCTTCCCCTGGAACACCATGCTTAAGTTTTTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATACATCCACCGGAGATGAACACCCAGTCCAACGGTACACCCCTGCGCCAGGGGTACCTGGCGCAggg                                                                                                                                         <  2:1262014/150‑3 (MQ=255)
             gTGCGTATCATTCCCGTGGAACACCATGCTTACGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCg                                                                                                                                         <  1:1119824/150‑1 (MQ=255)
             gTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCg                                                                                                                                         <  2:1655005/150‑1 (MQ=255)
                 gTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGtttt                                                                                                                                     >  2:659791/1‑150 (MQ=255)
                 gTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGtttt                                                                                                                                     <  1:1735115/150‑1 (MQ=255)
                  tATCATTCCCGTGGAAAACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCGGGGATTTCAACGGCGGCATCCATCCCCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCTGGCGCAGCGTTTTg                                                                                                                                    <  2:1489088/150‑1 (MQ=255)
                   aTCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGt                                                                                                                                   >  2:1844835/1‑150 (MQ=255)
                   aTCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGt                                                                                                                                   <  1:1097554/150‑1 (MQ=255)
                   aTCATTCCCGTGGAACACCATGCTTAAGTTAATCTCTGCATTCCGAAAAAATAAAATCTGGTATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGt                                                                                                                                   <  2:1243041/150‑1 (MQ=255)
                    tCATTCCCGTGGAACAACATGCTTAAGTTATTCTCTGCATTCATAAAAAATAAAAGCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGGCAGGTACCCCTGGCGCAGCGTTTTGtt                                                                                                                                  <  1:472564/150‑1 (MQ=255)
                     cATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTa                                                                                                                                 <  2:1972501/150‑1 (MQ=255)
                     cATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCAGGCGCAGCGTTTTGTTa                                                                                                                                 >  1:526431/1‑150 (MQ=255)
                      aTTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTAt                                                                                                                                >  2:432793/1‑150 (MQ=255)
                       ttCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACTGCGGCATCCATCCACCGGAGATGAAAACCCAGTCAAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCCGCGTTTTGGTAtt                                                                                                                               >  2:1052726/1‑150 (MQ=255)
                        tCCCGTGGTACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAAGGGCGGCTTCCATCCACCGGAGATGAAAACCCAGTCCAACGGAACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTATTGTTATTc                                                                                                                              <  2:588547/150‑1 (MQ=255)
                        tCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTc                                                                                                                              <  2:1034418/150‑1 (MQ=255)
                         cccGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTcc                                                                                                                             <  2:1905874/150‑1 (MQ=255)
                          ccGTGGAACACCATTCTTAAGTTTTTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCTGCATCCATCCACCGGAGATGAAATCCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCa                                                                                                                            <  1:923031/150‑1 (MQ=255)
                          ccGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCa                                                                                                                            <  1:923034/150‑1 (MQ=255)
                            gTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACTCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACt                                                                                                                          >  1:858457/1‑150 (MQ=255)
                             tGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTg                                                                                                                         <  2:1534726/150‑1 (MQ=255)
                               gAACACCATGCTGAAGTTATTCTCTGCATTCAGAAAATATAAAATCTGGGATTTCACCGGCGTCATCACTCCCCCGGAGATGAAAGCCCAGTCCAACGTTACACCCCTGCGCACGGGACCCCTGGCGCCGCGGTTTGGTATTccccgggt                                                                                                                       >  2:865300/1‑144 (MQ=255)
                                 acacCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAAc                                                                                                                     <  1:365/150‑1 (MQ=255)
                                   acCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGTATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAg                                                                                                                   >  1:36896/1‑150 (MQ=255)
                                   acCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTCCAACAGGCGCCGCGTGATCTTATTCCACTGCAAAAg                                                                                                                   >  1:1335785/1‑150 (MQ=255)
                                     cATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCa                                                                                                                 <  1:1415784/150‑1 (MQ=255)
                                          ttAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTg                                                                                                            >  2:1959493/1‑150 (MQ=255)
                                           tAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATGCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTgg                                                                                                           <  1:1826802/150‑1 (MQ=255)
                                           tAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTgg                                                                                                           >  2:722963/1‑150 (MQ=255)
                                             aGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATACATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGgcg                                                                                                         <  2:180542/150‑1 (MQ=255)
                                              gTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGgcgc                                                                                                        <  2:795633/150‑1 (MQ=255)
                                              gTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGAGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGgcgc                                                                                                        <  1:1301364/150‑1 (MQ=255)
                                                    ctctGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTCCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAgg                                                                                                  >  1:1345374/1‑150 (MQ=255)
                                                    ctctGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGTAgg                                                                                                  >  1:1857739/1‑150 (MQ=255)
                                                     tctGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGt                                                                                                 >  2:484811/1‑150 (MQ=255)
                                                              aGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGc                                                                                        <  1:985151/150‑1 (MQ=255)
                                                                aaaaaaTAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCAAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCTTATTGGCGCTGAAGGTGTGTTGTGCGt                                                                                      >  1:1339966/1‑150 (MQ=255)
                                                                      taaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACCGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGATATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGt                                                                                >  1:1426512/1‑150 (MQ=255)
                                                                         aatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAACCCCAGTCCAACGGTACACACCTGCGCCAGGTACCCCAGGCGCAGCGTGTTGGTATTCAACTGAAAAAGCATATTGGCGCGGATGGTGATTTGTGCGGTAGCGTCgg                                                                             >  1:968947/1‑150 (MQ=255)
                                                                         aatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGGTCCCCTGGGGCAGCGGTTTGTTTTTACACGGAAACACGAAATTTGCGCTGAAGGGGAGTTGTGCGTTAGCGTCgg                                                                             >  1:1470914/1‑150 (MQ=255)
                                                                          atCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACACCTGCGCCAGGTACCCCTGGAGCAGCGTTTTGTTATTCCCCTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGc                                                                            >  1:1425962/1‑150 (MQ=255)
                                                                              gtgATTTCAACGTCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCTTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATa                                                                        <  2:1294166/148‑1 (MQ=255)
                                                                                  tttCAACGGCGTCATGCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCTTATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGt                                                                    <  2:779533/150‑1 (MQ=255)
                                                                                  tttCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGt                                                                    <  1:1797974/150‑1 (MQ=255)
                                                                                     cAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTTCCCCTGGCGCAGCGGTTTGTTATTCCAATAAAACCACATATTGGCGCGGAAGGTGAGTTGTGCGTTTGCGTCGGCGATTAAGGAtt                                                                 >  2:523547/1‑150 (MQ=255)
                                                                                     cAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTAtt                                                                 >  2:1196506/1‑150 (MQ=255)
                                                                                      aaCGGCGGCATCCATCCACCGGAGATGAAACCCCAGTCCAACGGTAGACCCGTGCGCCAGGTTCCCCTGGCGCAGCGGTTTGTTATTCCTCTGAAACAGCATTTTGGCGGTGATGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTg                                                                <  2:1492385/150‑1 (MQ=255)
                                                                                      aaCGGCGCCATCCTTCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGGGACTTGTGCGTTAGCGTCGGCGATAAAGTATTg                                                                <  2:1059250/150‑1 (MQ=255)
                                                                                           cggcATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGCAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGGGTCGGCGATAAAGTATTGCGCgg                                                           >  1:1867687/1‑150 (MQ=255)
                                                                                           cggcATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCgg                                                           <  1:1959493/150‑1 (MQ=255)
                                                                                            ggcATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGc                                                          <  1:1061313/150‑1 (MQ=255)
                                                                                             gcATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGcc                                                         <  1:1505164/150‑1 (MQ=255)
                                                                                                 ccatccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGcc                                                     <  1:1842990/150‑1 (MQ=255)
                                                                                                  catccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTTGGCGATAAAGTATTGCGCGGCCAGCCg                                                    >  2:1123620/1‑150 (MQ=255)
                                                                                                    tccaCCGGAGATGAATACCCAGTCCAACGTTACACCCATGCGCCATGTACCCCTGGCGCAGCGGTTTGTTATTCCAGTGAAACAGCATATTGGCGCGGAAGGTGAGGTGTGCGTTAGCGTCGGCTATAAAGTATTGCGCGGCCAGCCGCt                                                  <  1:152398/150‑1 (MQ=255)
                                                                                                    tccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCt                                                  <  1:395727/150‑1 (MQ=255)
                                                                                                    tccaCCGGAGATGAAAACCCAGTCCAACGGAACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCt                                                  <  2:1622925/150‑1 (MQ=255)
                                                                                                        ccGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTATAGGCGGCGAAAAAGTATTTCGCGGCCAGCCGCTTAcc                                              >  2:1871507/1‑150 (MQ=255)
                                                                                                          ggAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCg                                            <  1:1356218/150‑1 (MQ=255)
                                                                                                            tgaTGAAAACCCAGTCCAAGGGTACACCCCTGCGCCAGGTACCCCTGGCTAAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTAGCCGTg                                          <  2:559178/149‑1 (MQ=255)
                                                                                                            agaTGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTg                                          <  2:1734810/150‑1 (MQ=255)
                                                                                                               tGAAACCCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTTAAACCAACTATTGGCGGTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAATTATTGCGCGGCCAGCCGCTTACCAGTGGTc                                       >  1:1254688/1‑150 (MQ=255)
                                                                                                               tGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCCCTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTc                                       >  1:237546/1‑150 (MQ=255)
                                                                                                               tGAAAACCCAGTCCAACGGTACACCCCTGCCCCAGGTACCCCTGGCGCAGCGTTTTGTTATTACACAGAAACAGCATATTGGCGCTGAAAGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTTCGCGGCCAGCCGCTTAacccgggta                                       >  2:1481821/1‑144 (MQ=255)
                                                                                                                           ccAACGGTACACCCCTGCGCCAGGTACCCCTGGCGAAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAAtgc                           <  2:1482671/150‑1 (MQ=255)
                                                                                                                            cAACGGTACACCCCTGCGCCAGGTACCCCGGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGGATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAAtgcg                          >  2:1191833/1‑149 (MQ=255)
                                                                                                                             aaCGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAAtgctg                         >  2:1991282/1‑150 (MQ=255)
                                                                                                                             aaCGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAAtgctg                         <  1:516030/150‑1 (MQ=255)
                                                                                                                              aCGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAAtgctgc                        <  1:1370738/150‑1 (MQ=255)
                                                                                                                               cGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGcc                       <  1:1133399/150‑1 (MQ=255)
                                                                                                                                   acacCCCTGCGCCAGGTACCCCGGGCGCAGCGGTTTGTTATTCCACTGCAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGtt                   <  1:1408872/150‑1 (MQ=255)
                                                                                                                                    cacTCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTc                  <  2:858457/150‑1 (MQ=255)
                                                                                                                                    cacCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAACCAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTc                  >  1:1642118/1‑150 (MQ=255)
                                                                                                                                    cacCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTc                  <  1:1656051/150‑1 (MQ=255)
                                                                                                                                       cccTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGACACAGCATATAGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCAcg               <  1:275539/150‑1 (MQ=255)
                                                                                                                                        gcTGCGCCAGGGGTACCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGGTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAACATGCTGCCTGTTCAcgc              >  1:1282876/2‑150 (MQ=255)
                                                                                                                                        gcTGCGCCAGGGGTACCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCAcgc              >  1:1267775/2‑150 (MQ=255)
                                                                                                                                        gcTGCGCCAGGGGTACCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCAcgc              >  1:884718/2‑150 (MQ=255)
                                                                                                                                         cTGCGCCAGGTACCCCTGGCGCAGGGGTTTGTTATTCGACTGAAAGAGCATATTGGCGCTGAAGGGGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCAcgcg             <  1:450722/150‑1 (MQ=255)
                                                                                                                                          tGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACgcgc            <  1:484811/150‑1 (MQ=255)
                                                                                                                                          tGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACgcgc            <  1:1006776/150‑1 (MQ=255)
                                                                                                                                           gcgcCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGcc           <  2:659799/150‑1 (MQ=255)
                                                                                                                                                 ggTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTc     <  1:630488/150‑1 (MQ=255)
                                                                                                                                                    aCCCCTGGCGGAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGCTGAGTTGAGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTCggg  <  1:316743/150‑1 (MQ=255)
                                                                                                                                                    aCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTCggg  <  1:2011807/150‑1 (MQ=255)
                                                                                                                                                    aCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTCggg  <  1:668733/150‑1 (MQ=255)
                                                                                                                                                    aCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTCggg  <  1:950465/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTCGGG  >  NC_000913/1706881‑1707178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: