Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,747,155 G→T 8.2% A9S (GCC→TCC)  ydhS → putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,747,1550GT 8.2% 269.6 / 2.3 97A9S (GCC→TCC) ydhSputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (53/33);  new base T (2/9);  total (55/42)
Fisher's exact test for biased strand distribution p-value = 8.72e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCC  >  NC_000913/1747006‑1747304
                                                                                                                                                     |                                                                                                                                                     
cAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAAATCGAAAGAATGCGTAAAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTg                                                                                                                                                       >  1:390421/1‑150 (MQ=255)
   aaTTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCg                                                                                                                                                    >  1:1670598/1‑150 (MQ=255)
      ttAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTAAACCAAACTTATCTGATAAAACTAAATTCGAAAGAATGCGTACAGGTATGTATCTATGTAAAATATTGCTATTCTGGGTTCCGGGc                                                                                                                                                 >  2:292996/1‑150 (MQ=255)
          tCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTAc                                                                                                                                             >  2:1351294/1‑150 (MQ=255)
           cAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCCACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTCGGTGACGGGCCTACg                                                                                                                                            >  2:1748405/1‑150 (MQ=255)
           aaaGGGCGCTAACCGCCCTTTTCATCCCTGTCTGAAATATCTCAAATTCTAAAAATCGCAACCAAACGTAGCGGATAACAATAAATTCGAAATAATGCGTACAGGTAATTAACAATGAAAAAAATTGCTATTGTCTTTGCCGGGACTACg                                                                                                                                            >  1:1010564/2‑150 (MQ=255)
             aGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACggg                                                                                                                                          <  1:1411223/150‑1 (MQ=255)
              gggCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACgggg                                                                                                                                         <  2:848632/150‑1 (MQ=255)
              gggCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACgggg                                                                                                                                         <  2:1103701/150‑1 (MQ=255)
              gggCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACgggg                                                                                                                                         <  2:1644453/150‑1 (MQ=255)
                gCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGAt                                                                                                                                       <  1:43907/150‑1 (MQ=255)
                gCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGAt                                                                                                                                       <  2:275841/150‑1 (MQ=255)
                gCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGAGGGTGCCGGGCCTACGGGGAt                                                                                                                                       >  1:1214652/1‑150 (MQ=255)
                      aGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTacac                                                                                                                                 <  1:1233062/150‑1 (MQ=255)
                      aGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGAGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTacac                                                                                                                                 >  2:333444/1‑150 (MQ=255)
                       gcgcCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGATATTGTGGGTGCCGGGCCTACGGGGATCTacaca                                                                                                                                >  1:1653282/1‑149 (MQ=255)
                        cgcCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGAAAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCt                                                                                                                               <  1:828977/150‑1 (MQ=255)
                          cccTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTa                                                                                                                             >  2:415488/1‑150 (MQ=255)
                             ttttCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATtc                                                                                                                          >  2:572191/1‑150 (MQ=255)
                              tttCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATtct                                                                                                                         <  1:1351294/150‑1 (MQ=255)
                                  aTCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTTCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCt                                                                                                                     <  1:965328/150‑1 (MQ=255)
                                    cccTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTc                                                                                                                   >  1:568761/1‑150 (MQ=255)
                                     ccTGTCTGAAATTTCTCAAATGCTAAAAATCTCAACCAAACTTCTCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTACCAATGAAAAAAATTGCTATGGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCt                                                                                                                  <  1:1337784/150‑1 (MQ=255)
                                       tGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTAc                                                                                                                <  1:910951/150‑1 (MQ=255)
                                       tGTCTGAAAAATCTCAAAATATAAAAATCTCAACCAAACTTATCTGAAAACACTAAAATCGAAAGACTGCGGACAGGTAAGTAAAAATTAAAATTTTTTTTTTTTTTGTTTCGGTGACTACGGTGATCTACACCTGAGGCTCGcggcgac                                                                                                                <  2:1412811/150‑7 (MQ=255)
                                          cTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATAGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGc                                                                                                             >  1:478230/1‑150 (MQ=255)
                                           tGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTTTTTTTTTTGTTTCGGGGACTACGGTGATCTACACCTTATTCTCGCTTCTACAGca                                                                                                            <  2:885066/150‑1 (MQ=255)
                                            gAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGcaa                                                                                                           >  1:1941835/1‑150 (MQ=255)
                                             aaaTTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGcaac                                                                                                          <  1:683508/150‑1 (MQ=255)
                                             aaaTTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGcaac                                                                                                          >  2:1285380/1‑150 (MQ=255)
                                              aaTTTCTCAAAATCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGAACAGCTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGcaaca                                                                                                         <  1:735140/150‑1 (MQ=255)
                                                   ctcAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTc                                                                                                    >  2:713608/1‑150 (MQ=255)
                                                    tcAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTcc                                                                                                   >  1:1408218/1‑150 (MQ=255)
                                                     cAAATTCTAAAAATCTCAACCAATCTTATATGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAATAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACAACTTATTCTCGCTTCTACAGCAACAAACTCCa                                                                                                  <  1:173969/150‑1 (MQ=255)
                                                       aaTTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACATTATTCTCGCTTCTACAGCAACAAACTCCACt                                                                                                >  1:468479/1‑150 (MQ=255)
                                                        aTTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACtt                                                                                               >  1:1721935/1‑150 (MQ=255)
                                                           cTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACtttct                                                                                            >  2:836114/1‑150 (MQ=255)
                                                            tAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACtttcta                                                                                           >  2:179929/1‑150 (MQ=255)
                                                             aaaaaTCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACATCAACAAACTCCACtttctat                                                                                          >  2:75860/1‑150 (MQ=255)
                                                              aaaaTCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAATTAACAATGAAAAAAATTGCTTTTTTTGTTTCGGGGACTACGGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACtttctatt                                                                                         <  2:516785/150‑1 (MQ=255)
                                                              aaaaTCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACtttctatt                                                                                         <  1:1717849/150‑1 (MQ=255)
                                                              aaaaTCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCATACGGGGATCTAAACCTTATTCTCGCTTCTACAGCAACAAACTCCACtttctatt                                                                                         >  1:49074/1‑150 (MQ=255)
                                                                aaTCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACtttctatttc                                                                                       <  2:2000274/150‑1 (MQ=255)
                                                                aaTCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGATATTTTTGTTGCGGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACtttctatttc                                                                                       <  2:470612/150‑1 (MQ=255)
                                                                  tctcAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACtttctatttcta                                                                                     <  1:1470180/150‑1 (MQ=255)
                                                                       aCCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTc                                                                                >  1:475693/1‑150 (MQ=255)
                                                                        ccAAACTTATCTGATAACACTAAAATCGAAAGAATGCGTACATGTAAGTAACAATGAATATTTTTGTTTTTTTTGTTTCGGGGACTACGGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCg                                                                               <  2:1990953/150‑1 (MQ=255)
                                                                             cTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAg                                                                          >  2:196599/1‑150 (MQ=255)
                                                                              ttATCTGATAACACTAAATTCGAAAGACTGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTTTGGGTGCAGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAgg                                                                         <  1:43051/150‑1 (MQ=255)
                                                                               tctCTGATAACACTAAATTCGAAAGAATGCGAACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGc                                                                        <  1:1984851/148‑1 (MQ=255)
                                                                               tATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGc                                                                        <  1:452742/150‑1 (MQ=255)
                                                                                 tCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTg                                                                      >  1:466780/1‑150 (MQ=255)
                                                                                  cTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTTCCGAGCCTACGGGGATCTACACCTTATTATCGCTTCTACAGCAACAAACTCAACTTTCTATTTCTATATTCTAGCAGGCTgc                                                                     >  1:322743/1‑149 (MQ=255)
                                                                                    gATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCCACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACg                                                                   >  2:1629076/1‑150 (MQ=255)
                                                                                    gATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACg                                                                   >  1:755423/1‑150 (MQ=255)
                                                                                    gATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACg                                                                   >  2:1104617/1‑150 (MQ=255)
                                                                                    gATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACg                                                                   <  1:118194/150‑1 (MQ=255)
                                                                                      tAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAg                                                                 >  2:1283204/1‑150 (MQ=255)
                                                                                      tAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAg                                                                 <  1:417506/150‑1 (MQ=255)
                                                                                      tAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATATCTATCTTCGAGCAGGCTGACGAg                                                                 >  1:970456/1‑150 (MQ=255)
                                                                                        acacTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAATTTTTTTTTTTTGTTTCGGGGACTACGGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGc                                                               <  2:1330601/150‑1 (MQ=255)
                                                                                        acacTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGc                                                               >  2:1291996/1‑150 (MQ=255)
                                                                                          acTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCg                                                             >  1:1934318/1‑150 (MQ=255)
                                                                                           cTACATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAATTTTGTTTTTTTTGTTTCGGGGACTACGGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCgg                                                            <  2:970039/150‑1 (MQ=255)
                                                                                           cTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCgg                                                            <  1:578003/150‑1 (MQ=255)
                                                                                             aaaTTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGgtg                                                          >  2:206718/1‑150 (MQ=255)
                                                                                                 tCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCgg                                                      >  1:1686552/1‑150 (MQ=255)
                                                                                                  cGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGCCGAGGCCGGTGTCggg                                                     >  1:1741699/1‑150 (MQ=255)
                                                                                                  cGAAACAATGCGTACAGGAAAGTAACTATGAAAAAAATTGCTATTGTTGGTGCCGGGCCTAAGGGGATCTACCCCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCAATTTCTATCTTCGATCAGTATGACGAGGAAGGTGTCggg                                                     >  2:337169/1‑150 (MQ=255)
                                                                                                   gAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGa                                                    >  1:167676/1‑150 (MQ=255)
                                                                                                    aaaGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGAt                                                   >  1:1689079/1‑150 (MQ=255)
                                                                                                     aaGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATg                                                  <  1:572191/150‑1 (MQ=255)
                                                                                                        aaagCGTACACGTAAGTACCAATGAATAAATTTGATATTGTGGGTTCGGGGCCTACGGGGATCTACACCTTATTCTCGCTTATACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCa                                               <  2:100883/147‑1 (MQ=255)
                                                                                                        aaTGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCa                                               >  1:858499/1‑150 (MQ=255)
                                                                                                             gtaaatgtaagtaaAAATTAAAAAAATTTTTTTTTTTGTTTAGGTGACTACGGTGATCTACAACTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAg                                          <  2:1615087/144‑1 (MQ=255)
                                                                                                              tACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGt                                         >  2:1043742/1‑150 (MQ=255)
                                                                                                              tACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAACAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGt                                         >  2:1961449/1‑150 (MQ=255)
                                                                                                                  ggTAAGTAACAATTAAAAAAATTTCTATTGTGGGTTCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGtgatg                                     <  2:392356/150‑1 (MQ=255)
                                                                                                                  ggTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGtgatg                                     >  1:1951345/1‑150 (MQ=255)
                                                                                                                  ggTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGtgatg                                     >  2:873448/1‑150 (MQ=255)
                                                                                                                    taagtaaCAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAg                                   <  1:1044351/150‑1 (MQ=255)
                                                                                                                         aaCAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGaaaa                              >  2:1841580/1‑150 (MQ=255)
                                                                                                                          aCAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATTCAGTGATGAGGAAAAc                             >  2:855989/1‑150 (MQ=255)
                                                                                                                           cAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACt                            >  2:747565/1‑150 (MQ=255)
                                                                                                                              tGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGTAAAACTCaa                         >  1:1420118/1‑150 (MQ=255)
                                                                                                                               gAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCaaa                        >  2:457898/1‑150 (MQ=255)
                                                                                                                                aaaaaaaTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCaaaa                       <  1:1283204/150‑1 (MQ=255)
                                                                                                                                         gCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTg              <  2:755423/150‑1 (MQ=255)
                                                                                                                                          cTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTgg             <  1:828723/150‑1 (MQ=255)
                                                                                                                                          cTAGTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAATACTCAAAAATGATGCTgg             >  2:1028588/1‑150 (MQ=255)
                                                                                                                                           tATTGTGGGTGCCGGGCCTACGGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCAATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGc            <  2:1686367/150‑1 (MQ=255)
                                                                                                                                            aTTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCa           >  1:1834836/1‑150 (MQ=255)
                                                                                                                                            aTTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCa           >  2:716119/1‑150 (MQ=255)
                                                                                                                                               gtgGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAat        >  2:1374726/1‑150 (MQ=255)
                                                                                                                                                ggggTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCTGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAACTGATGCTGGCAAata       <  1:795008/149‑1 (MQ=255)
                                                                                                                                                 gggTGCCGGGCCTACGGGGATCTACCCCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGCGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAatat      >  1:1097133/1‑150 (MQ=255)
                                                                                                                                                 gggTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAatat      >  2:1825803/1‑150 (MQ=255)
                                                                                                                                                  ggTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATAtt     <  2:568761/150‑1 (MQ=255)
                                                                                                                                                     gCCGGGCCTACGGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGcc  <  2:478230/150‑1 (MQ=255)
                                                                                                                                                     gCCGGGCCTACGGGGATCTACCCCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGCTGCCATACAGTGATGAGGATAACTCAAAAATGATGCTGGCAAATATTGcc  >  2:309278/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTGCC  >  NC_000913/1747006‑1747304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑