Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,807,319 T→C 5.1% intergenic (+20/‑81) pfkB → / → ydiZ 6‑phosphofructokinase II/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,807,3190TC 5.1% 423.3 / 9.0 138intergenic (+20/‑81)pfkB/ydiZ6‑phosphofructokinase II/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (59/72);  new base C (4/3);  total (63/75)
Fisher's exact test for biased strand distribution p-value = 7.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.16e-01

Only 100 of 139 total aligned reads displayed.

GAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAAT  >  NC_000913/1807171‑1807467
                                                                                                                                                    |                                                                                                                                                    
gAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCAtt                                                                                                                                                     <  1:1921536/150‑1 (MQ=255)
 aaaaTGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTg                                                                                                                                                    >  2:1852107/1‑150 (MQ=255)
    aTGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTgggg                                                                                                                                                 <  1:648492/150‑1 (MQ=255)
     tGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTggggg                                                                                                                                                <  2:44349/150‑1 (MQ=255)
     tGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTggggg                                                                                                                                                >  1:1368625/1‑150 (MQ=255)
      tccTCTCTTGAAGAGATGGTTCGTTTTGTCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGTGAACACGTATGTGCTCCCATGACGATACGCAAAAAATTTACGCTTAACTTTCCCGCTAACAAAATCATTCCCCCATCATTGGGGGa                                                                                                                                               <  1:376668/149‑1 (MQ=255)
      gCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGa                                                                                                                                               <  2:2003831/150‑1 (MQ=255)
      gCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGa                                                                                                                                               <  2:214368/150‑1 (MQ=255)
         tctctTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAatc                                                                                                                                            <  2:771105/150‑1 (MQ=255)
         tctctTGAAGAGATGGTGCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGAAACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAATGCTGGGGGAatg                                                                                                                                            <  2:343523/150‑2 (MQ=255)
           tctTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAATGCTGGGGGAatgtt                                                                                                                                          <  2:1913866/150‑4 (MQ=255)
           tctTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAATGCTGGGGGAatgtt                                                                                                                                          <  1:710603/150‑4 (MQ=255)
            ctTGAAGAGATGGTTCGATTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGCTACTCAAAAAATTTACGCTTACCTATCCCGCTAACAAAAACATTCCCCCAGCATTGCGGGAatcagc                                                                                                                                         >  2:1552017/1‑148 (MQ=255)
              tGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCAc                                                                                                                                       <  1:1161368/150‑1 (MQ=255)
              tGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCAc                                                                                                                                       <  1:1859657/150‑1 (MQ=255)
              tGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCACCAGCATTGGGGGAATCATCAc                                                                                                                                       >  2:1509818/1‑150 (MQ=255)
               gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCAcc                                                                                                                                      <  1:435612/150‑1 (MQ=255)
                aaGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCa                                                                                                                                     <  1:706765/150‑1 (MQ=255)
                  gagaTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATAAGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAAc                                                                                                                                   <  2:1138020/150‑1 (MQ=255)
                   tgaTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAAcc                                                                                                                                  <  2:1859659/149‑1 (MQ=255)
                   agaTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAAcc                                                                                                                                  <  1:913229/150‑1 (MQ=255)
                   agaTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAAcc                                                                                                                                  <  2:1246264/150‑1 (MQ=255)
                   agaTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAAcc                                                                                                                                  <  2:1262736/150‑1 (MQ=255)
                     aTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTg                                                                                                                                <  2:1445033/150‑1 (MQ=255)
                                  gCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGtt                                                                                                                   <  2:1982397/150‑1 (MQ=255)
                                    ggaGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAACACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTtc                                                                                                                 <  1:76314/148‑1 (MQ=255)
                                       gCTGCGTTGAGTGCAGCCACACTCAATCAGTGAACACGTCAGTGCTCCCATGACGATACGCAAAAATTTTACGCTTACCTTTCCAGCTAAAAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTcc                                                                                                              <  2:87235/150‑1 (MQ=255)
                                        cTGCGGGGAGTGCAGCCACTCTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCg                                                                                                             <  2:727488/150‑1 (MQ=255)
                                        cTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCg                                                                                                             <  1:231587/150‑1 (MQ=255)
                                        cTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCg                                                                                                             >  1:1039139/1‑150 (MQ=255)
                                         tGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGa                                                                                                            >  1:1408847/1‑150 (MQ=255)
                                          gCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCgcc                                                                                                           >  1:1128158/1‑148 (MQ=255)
                                           cGGGGAGTGCAGCCACACGCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAACACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACt                                                                                                          <  1:100225/150‑1 (MQ=255)
                                            ggggAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTa                                                                                                         <  2:1386621/150‑1 (MQ=255)
                                            ggggAGTGCAGACACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCACCCTGTCGGCAACGCGGTTCTCCGGCTa                                                                                                         >  1:349268/1‑150 (MQ=255)
                                               gAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGc                                                                                                      <  1:1477147/150‑1 (MQ=255)
                                                aGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCt                                                                                                     >  2:1594860/1‑150 (MQ=255)
                                                aGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTACCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCt                                                                                                     >  2:1603168/1‑150 (MQ=255)
                                                 gTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTc                                                                                                    <  2:194250/150‑1 (MQ=255)
                                                    cAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCaaa                                                                                                 >  1:1514129/1‑150 (MQ=255)
                                                    cAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCaaa                                                                                                 >  1:434327/1‑150 (MQ=255)
                                                    cAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCaaa                                                                                                 >  1:832731/1‑150 (MQ=255)
                                                      gCCACACTCAATCAGGGATCACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTAGCTTTCCCGCTAACAAAAACAATCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCATAAg                                                                                               <  2:1288710/150‑1 (MQ=255)
                                                        cacaCTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTc                                                                                             <  2:426181/150‑1 (MQ=255)
                                                         acacTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCa                                                                                            <  1:984410/150‑1 (MQ=255)
                                                          cacTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCAt                                                                                           <  1:1652862/150‑1 (MQ=255)
                                                           acTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCCGCAACGCGTTTCTCCGACTATGCTCAAAAGTCAtg                                                                                          <  1:62658/150‑1 (MQ=255)
                                                            cTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCAtgt                                                                                         <  1:1684423/150‑1 (MQ=255)
                                                            cTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCAtgt                                                                                         >  1:980411/1‑150 (MQ=255)
                                                            cTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCAtgt                                                                                         <  1:1236114/150‑1 (MQ=255)
                                                            cTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCAtgt                                                                                         >  2:2247/1‑150 (MQ=255)
                                                               aaTCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCACCCTGTCGGCACCGCGTTTCTCCGACTATGCTCAAAAGTCAGGTGAt                                                                                      >  1:1190415/1‑150 (MQ=255)
                                                                     ggAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCACCGACTATGCTCAAAAGTCATGTGATAACaaa                                                                                >  1:1863328/1‑150 (MQ=255)
                                                                      gAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCTACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATTACAAAg                                                                               >  1:714255/1‑150 (MQ=255)
                                                                        acacGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAggg                                                                             <  2:1994978/150‑1 (MQ=255)
                                                                         cacGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAgggg                                                                            <  2:283177/150‑1 (MQ=255)
                                                                         cacGTCTGTGCTCCAATGACGATACGCAAAAAATTTACGCTTACATTTCCCGCTAACAAAAACATTCCCCCAGCATTTGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAgggg                                                                            <  1:85860/150‑1 (MQ=255)
                                                                           cGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTg                                                                          >  1:1595516/1‑150 (MQ=255)
                                                                           cGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTg                                                                          <  2:558831/150‑1 (MQ=255)
                                                                           cGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTg                                                                          <  2:353867/150‑1 (MQ=255)
                                                                              cTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCCGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAAc                                                                       <  2:1009272/150‑1 (MQ=255)
                                                                               tgtgCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACt                                                                      >  1:1606882/1‑150 (MQ=255)
                                                                                               tACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATc                                                      <  1:1012441/150‑1 (MQ=255)
                                                                                               tACGCAAAAAATTTACGATTACCTTTCCCGCTAACAAAAACATTCCACCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATc                                                      <  1:1144322/150‑1 (MQ=255)
                                                                                                  gCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTg                                                   >  2:1729227/1‑150 (MQ=255)
                                                                                                   cAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGt                                                  >  1:577889/1‑150 (MQ=255)
                                                                                                    aaaaaaTTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCACCGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTc                                                 >  2:133873/1‑150 (MQ=255)
                                                                                                     aaaaaTTTACGCTTACCTTTCCCGCTAACAAAACCATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGTCAACGCGTTTCTCCGACTATGCTCACAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTcc                                                <  1:1305995/150‑1 (MQ=255)
                                                                                                     aaaaaTTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTcc                                                <  1:423118/150‑1 (MQ=255)
                                                                                                         aTTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTa                                            <  2:1384759/150‑1 (MQ=255)
                                                                                                            tACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGt                                         <  1:1303982/150‑1 (MQ=255)
                                                                                                            tACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGt                                         <  2:812084/150‑1 (MQ=255)
                                                                                                                cTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAATGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATa                                     <  1:2247/150‑1 (MQ=255)
                                                                                                                   aCCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAAcc                                  >  2:1800232/1‑150 (MQ=255)
                                                                                                                   aCCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAAcc                                  <  2:1775497/150‑1 (MQ=255)
                                                                                                                   aCCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAAcc                                  >  2:1573080/1‑150 (MQ=255)
                                                                                                                      tttCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTc                               <  2:1112143/150‑1 (MQ=255)
                                                                                                                       ttCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCa                              <  2:1964958/150‑1 (MQ=255)
                                                                                                                        tCCCTCTAACAAAAACATTCCCCCAGCATTGTGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGTTAAGAAAGGGGTGAACTATTGCCAGTGGCGAGCTTGTCCGTTAGTTCATAACCGTCAt                             >  2:1767393/1‑150 (MQ=255)
                                                                                                                         cccGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCGCCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGt                           >  2:854037/1‑150 (MQ=255)
                                                                                                                          ccGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGt                           >  2:1098771/1‑150 (MQ=255)
                                                                                                                             cTAACAAAAACAATCCCCCAGCATTTGGGGAATCATCACCAACCTGTCTGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATATCAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGc                        <  2:300306/150‑1 (MQ=255)
                                                                                                                               aaCAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCAGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCAt                      <  1:1852107/150‑1 (MQ=255)
                                                                                                                                 cAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGa                    >  1:16993/1‑150 (MQ=255)
                                                                                                                                 cAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTACGTTATGTCATAACCGTCATGTTGCATGa                    >  1:790667/1‑150 (MQ=255)
                                                                                                                                 cAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGCCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGa                    >  2:935896/1‑150 (MQ=255)
                                                                                                                                 cAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTAAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGa                    >  1:1081864/1‑150 (MQ=255)
                                                                                                                                 cAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTATCTCCGACTATGCTCAAAAGTCAAGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCAGTTATGTCATAACCGTCATCTTGCATGa                    >  1:296013/1‑150 (MQ=255)
                                                                                                                                  aaaaaCATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAg                   >  1:517566/1‑150 (MQ=255)
                                                                                                                                      tgaTTCCCCCAATGCTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATa               >  1:1963282/3‑150 (MQ=255)
                                                                                                                                       gaTTCCCCCAATGCTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATAc              >  1:3681/2‑150 (MQ=255)
                                                                                                                                        aTTCCCCCAATGCTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACa             >  2:927668/1‑150 (MQ=255)
                                                                                                                                           cccccAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTg          <  1:1972596/150‑1 (MQ=255)
                                                                                                                                            ccccAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGa         >  1:1933867/1‑150 (MQ=255)
                                                                                                                                             cccAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATTGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGAc        >  2:1189352/1‑150 (MQ=255)
                                                                                                                                             cccAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCAGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATTACCGTCATGGTGCATGAGGATACAGTGAc        >  1:295580/1‑150 (MQ=255)
                                                                                                                                              ccctcATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATTCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACt       <  1:1475061/146‑1 (MQ=255)
                                                                                                                                              ccAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCTTGAGGATACATTGACt       >  1:584893/1‑150 (MQ=255)
                                                                                                                                               cAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTg      >  1:1894707/1‑150 (MQ=255)
                                                                                                                                                   aTTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAAt  <  2:355157/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAAT  >  NC_000913/1807171‑1807467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: