Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,808,680 T→A 5.2% intergenic (+24/+17) yniA → / ← yniB fructosamine kinase family protein/putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,808,6800TA 5.2% 454.3 / 8.0 151intergenic (+24/+17)yniA/yniBfructosamine kinase family protein/putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (73/69);  new base A (3/5);  total (76/75)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

Only 100 of 151 total aligned reads displayed.

CTGCCGATTTCCTTGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACG  >  NC_000913/1808532‑1808828
                                                                                                                                                    |                                                                                                                                                    
cTGCCGATTTCCTTGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGGCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTc                                                                                                                                                     <  2:1068065/150‑1 (MQ=255)
 tGCCGATTTCCTTGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTcc                                                                                                                                                    >  1:1370510/1‑150 (MQ=255)
        ttCCTTGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGTGGTCAGCATTTGGGTATTGCTCAGCAGTCATTGGCTCGATTATTAGCAGCATGATAGGGGTGGAGGATAATGGCCGCTCCGTGCGGc                                                                                                                                             <  2:1485466/150‑1 (MQ=255)
         tCCTTGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGcc                                                                                                                                            <  2:400415/150‑1 (MQ=255)
         tCCTTGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGcc                                                                                                                                            >  1:1683371/1‑150 (MQ=255)
           cTTGAACGTCAACCGGTTTACCAACTCTACACGCTTTTAAATCGTGCAAGGTTATTTTGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCACGGAGCGGccat                                                                                                                                          <  2:1799963/150‑3 (MQ=255)
           cTTGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCtt                                                                                                                                          >  2:1745072/1‑150 (MQ=255)
           cTTGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCACGGAGCGGccat                                                                                                                                          <  1:795642/150‑3 (MQ=255)
            ttGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCttt                                                                                                                                         >  2:677956/1‑150 (MQ=255)
             tGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGAGGTCAGCATTTGGTTATTGCGCAGCAGTCATTGGATAGATTATTAGCAGCAAGATATGGCTTGTGGATAATGGCCGCTCCGTGCGGCCtttt                                                                                                                                        >  2:1893571/1‑150 (MQ=255)
             tGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGGTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCgttt                                                                                                                                        >  2:1917809/1‑150 (MQ=255)
             tGAAAGTCAACCGGTTTACCAACTATACACGCTGTTAGATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCtttt                                                                                                                                        <  2:735029/150‑1 (MQ=255)
              gAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTg                                                                                                                                       <  2:1636206/150‑1 (MQ=255)
              gAACGGCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTg                                                                                                                                       <  1:1459117/150‑1 (MQ=255)
               aaCGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGa                                                                                                                                      <  1:1247903/150‑1 (MQ=255)
               aaCGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCACGGAGCGGCCattatc                                                                                                                                      <  1:505893/150‑4 (MQ=255)
                aCGTCCACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGAt                                                                                                                                     <  2:884659/150‑1 (MQ=255)
                aCGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGAt                                                                                                                                     <  1:1027336/150‑1 (MQ=255)
                 cGTCACCCGGTTTACAAACTCTACACGCTGTTAACTCGTGCAAGGTTAGTTGGCGGTCAGCATTTGGTTCTTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGGTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGAtt                                                                                                                                    <  1:1288570/150‑1 (MQ=255)
                 cGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCTGCCTTTTGAtt                                                                                                                                    >  2:1299461/1‑150 (MQ=255)
                 cGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTGTTGAtt                                                                                                                                    >  2:1808271/1‑150 (MQ=255)
                 cGTCAACCGGTTTAACAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGAtt                                                                                                                                    >  1:616084/1‑150 (MQ=255)
                  gTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATta                                                                                                                                   <  2:1898629/150‑1 (MQ=255)
                    cAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGTGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATtaaa                                                                                                                                 >  2:492070/1‑150 (MQ=255)
                    cAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATtaaa                                                                                                                                 >  2:1497981/1‑150 (MQ=255)
                     aaCCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATtaaat                                                                                                                                <  2:844350/150‑1 (MQ=255)
                     aaCCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATtaaat                                                                                                                                >  1:771834/1‑150 (MQ=255)
                         ggTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTTATTAAATAAAc                                                                                                                            >  1:30370/1‑150 (MQ=255)
                         ggTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAAc                                                                                                                            >  2:1091827/1‑150 (MQ=255)
                          gTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCATCATGATATGGGTTGAGGATAATGGCAGCTCCGTGCGGCCTTTTGATTAAATAAAcc                                                                                                                           >  1:960442/1‑150 (MQ=255)
                              aCCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAg                                                                                                                       <  2:1117284/150‑1 (MQ=255)
                               ccAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGc                                                                                                                      >  2:1962847/1‑150 (MQ=255)
                               ccAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGc                                                                                                                      >  2:1281841/1‑150 (MQ=255)
                                  aCTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCATCATGATATGGGTTGAGGATAATGGCCGCTCCTTGCGGCCTTTTGATTAAATAAACCCAAGCagt                                                                                                                   >  1:268641/1‑149 (MQ=255)
                                   ctctACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCagag                                                                                                                  <  2:71626/150‑1 (MQ=255)
                                       ccacGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATTGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGaaaa                                                                                                              >  1:1257424/2‑150 (MQ=255)
                                        cacGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAg                                                                                                             >  1:535445/1‑150 (MQ=255)
                                        cacGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAg                                                                                                             >  1:709403/1‑150 (MQ=255)
                                        cacGCTCTTAAATCGTGCAAGGTTATTTGGCGGGCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAg                                                                                                             <  2:381919/150‑1 (MQ=255)
                                         acGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGa                                                                                                            >  1:446131/1‑150 (MQ=255)
                                          cGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGaa                                                                                                           >  2:1750626/1‑150 (MQ=255)
                                          cGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGaa                                                                                                           <  1:837939/150‑1 (MQ=255)
                                            cTGTTAAATCGTGCTTGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAaca                                                                                                         <  2:1848855/150‑1 (MQ=255)
                                             tGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGAATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAacac                                                                                                        <  1:78906/150‑1 (MQ=255)
                                              gTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAAcaca                                                                                                       >  2:1848914/1‑150 (MQ=255)
                                                      gTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTgc                                                                                               >  1:645234/1‑150 (MQ=255)
                                                         cAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTgcagc                                                                                            >  1:226001/1‑150 (MQ=255)
                                                         cAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACAAAACCTgcagc                                                                                            >  2:250314/1‑150 (MQ=255)
                                                         cAAGGTTAGTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATACTGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTgcagc                                                                                            <  2:1530741/150‑1 (MQ=255)
                                                         aaaGGTTATTTGGCGGGCAGCTTTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGGTGCGTATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACTTAACCTgcagc                                                                                            <  2:874934/149‑1 (MQ=255)
                                                           aGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGAGTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCaa                                                                                          >  2:287400/1‑150 (MQ=255)
                                                             gTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATa                                                                                        <  2:1136970/150‑1 (MQ=255)
                                                              ttATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATAc                                                                                       <  1:1439502/150‑1 (MQ=255)
                                                                 tttGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACaaa                                                                                    <  2:1734787/150‑1 (MQ=255)
                                                                   tGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATa                                                                                  >  2:1002205/1‑150 (MQ=255)
                                                                   tGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATa                                                                                  <  2:100088/150‑1 (MQ=255)
                                                                       ggTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCg                                                                              >  1:256957/1‑150 (MQ=255)
                                                                         tCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGc                                                                            >  2:563071/1‑150 (MQ=255)
                                                                          cAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCa                                                                           >  2:1107145/1‑150 (MQ=255)
                                                                          cAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCa                                                                           <  1:1402328/150‑1 (MQ=255)
                                                                            gCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAgg                                                                         >  1:1120025/1‑150 (MQ=255)
                                                                            gCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAgg                                                                         <  2:1603248/150‑1 (MQ=255)
                                                                             cATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGa                                                                        >  2:1026369/1‑150 (MQ=255)
                                                                             cATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGGTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGa                                                                        <  2:1737660/150‑1 (MQ=255)
                                                                             cATTTGGTTATTGCTCAGCAGTCATTCGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAACAACACATAACATGCAGCAATACAAATAACCGCCAgca                                                                        >  1:948424/1‑148 (MQ=255)
                                                                               tttGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATTGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGata                                                                      <  1:1869925/150‑1 (MQ=255)
                                                                                 tGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGAtata                                                                    >  2:430808/1‑150 (MQ=255)
                                                                                   gTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACa                                                                  <  2:47994/150‑1 (MQ=255)
                                                                                   gTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACa                                                                  <  1:1109511/150‑1 (MQ=255)
                                                                                    ttATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAg                                                                 >  1:544131/1‑150 (MQ=255)
                                                                                     tATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGt                                                                <  1:1750626/150‑1 (MQ=255)
                                                                                      aTTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTg                                                               >  2:904028/1‑150 (MQ=255)
                                                                                      aTTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTg                                                               >  2:959761/1‑150 (MQ=255)
                                                                                       ttGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTga                                                              >  2:154772/1‑150 (MQ=255)
                                                                                       ttGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTga                                                              <  2:843997/150‑1 (MQ=255)
                                                                                        tGCTCATGAGTCATTGGCTAGATTATTATCAGCATTATATTGGTTGATGATAATGGCCGCGCCGTGCGGCCTTTTGATTAAAAAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTgag                                                             <  1:1775279/150‑1 (MQ=255)
                                                                                          cTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGaa                                                           >  1:1319510/1‑150 (MQ=255)
                                                                                            cagcagTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGaaaa                                                         >  1:1943706/1‑150 (MQ=255)
                                                                                                   cATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAg                                                  <  1:1564360/150‑1 (MQ=255)
                                                                                                     ttGGATAGATTATAAGCAGCATGATATGGGTTGAGGATACTGGCCGCTCCGTGCGGCCTTTTGGTTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGa                                                <  1:1695359/150‑1 (MQ=255)
                                                                                                       ggATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGaaa                                              >  2:568138/1‑150 (MQ=255)
                                                                                                         aTAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAAt                                            <  2:1723210/150‑1 (MQ=255)
                                                                                                            gATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGt                                         >  2:1587069/1‑150 (MQ=255)
                                                                                                            gATTATTAGCAGCATGATATGGGATGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAATAACACATAACCTGCAGAAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAAAAGt                                         >  1:128691/1‑150 (MQ=255)
                                                                                                             attattAGCAGCATGATATGGGTTGAGGATAATGGCCTCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTa                                        <  1:1046034/150‑1 (MQ=255)
                                                                                                             attattAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTa                                        <  1:217023/150‑1 (MQ=255)
                                                                                                             attattAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTa                                        >  1:807244/1‑150 (MQ=255)
                                                                                                                   agcagcATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGc                                  <  1:925121/150‑1 (MQ=255)
                                                                                                                     cagcaTGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCgg                                >  1:972548/1‑150 (MQ=255)
                                                                                                                       gcaTGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGaa                              <  2:453809/150‑1 (MQ=255)
                                                                                                                         aTGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGaaca                            <  1:1325203/150‑1 (MQ=255)
                                                                                                                           tatatGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGaacaac                          <  1:1659322/149‑1 (MQ=255)
                                                                                                                              atGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAAt                       >  1:1721428/1‑150 (MQ=255)
                                                                                                                                  gTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACgg                   >  2:876376/1‑150 (MQ=255)
                                                                                                                                     gatgATAATGTCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGAc                <  1:1430959/147‑1 (MQ=255)
                                                                                                                                      aGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACAAAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACt               >  1:1785693/1‑150 (MQ=255)
                                                                                                                                          aaaaggCCGCACGGAGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATTCTGGACTCatt           >  1:305289/5‑148 (MQ=255)
                                                                                                                                           aaTGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATACCCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAAtt          <  2:1069223/150‑1 (MQ=255)
                                                                                                                                            aTGGCCGCTCCGTACGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAAttt         >  2:676551/1‑150 (MQ=255)
                                                                                                                                                   cTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCAcg  <  1:1745072/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTGCCGATTTCCTTGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACG  >  NC_000913/1808532‑1808828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: