Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,808,684 T→A 5.4% intergenic (+28/+13) yniA → / ← yniB fructosamine kinase family protein/putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,808,6840TA 5.4% 431.9 / 10.9 148intergenic (+28/+13)yniA/yniBfructosamine kinase family protein/putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (72/68);  new base A (3/5);  total (75/73)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.14e-01

Only 100 of 151 total aligned reads displayed.

CCGATTTCCTTGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTA  >  NC_000913/1808535‑1808833
                                                                                                                                                     |                                                                                                                                                     
ccGATTTCCTTGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGt                                                                                                                                                       <  2:680188/150‑1 (MQ=255)
     ttCCTTGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGTGGTCAGCATTTGGGTATTGCTCAGCAGTCATTGGCTCGATTATTAGCAGCATGATAGGGGTGGAGGATAATGGCCGCTCCGTGCGGc                                                                                                                                                  <  2:1485466/150‑1 (MQ=255)
      tCCTTGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGcc                                                                                                                                                 >  1:1683371/1‑150 (MQ=255)
      tCCTTGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGcc                                                                                                                                                 <  2:400415/150‑1 (MQ=255)
        cTTGAACGTCAACCGGTTTACCAACTCTACACGCTTTTAAATCGTGCAAGGTTATTTTGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCACGGAGCGGccat                                                                                                                                               <  2:1799963/150‑3 (MQ=255)
        cTTGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCtt                                                                                                                                               >  2:1745072/1‑150 (MQ=255)
        cTTGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCACGGAGCGGccat                                                                                                                                               <  1:795642/150‑3 (MQ=255)
         ttGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCttt                                                                                                                                              >  2:677956/1‑150 (MQ=255)
          tGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGAGGTCAGCATTTGGTTATTGCGCAGCAGTCATTGGATAGATTATTAGCAGCAAGATATGGCTTGTGGATAATGGCCGCTCCGTGCGGCCtttt                                                                                                                                             >  2:1893571/1‑150 (MQ=255)
          tGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGGTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCgttt                                                                                                                                             >  2:1917809/1‑150 (MQ=255)
          tGAAAGTCAACCGGTTTACCAACTATACACGCTGTTAGATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCtttt                                                                                                                                             <  2:735029/150‑1 (MQ=255)
           gAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTg                                                                                                                                            <  2:1636206/150‑1 (MQ=255)
           gAACGGCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTg                                                                                                                                            <  1:1459117/150‑1 (MQ=255)
            aaCGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGa                                                                                                                                           <  1:1247903/150‑1 (MQ=255)
            aaCGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCACGGAGCGGCCattatc                                                                                                                                           <  2:880440/150‑4 (MQ=255)
            aaCGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCACGGAGCGGCCattatc                                                                                                                                           <  1:505893/150‑4 (MQ=255)
             aCGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGAt                                                                                                                                          <  1:1027336/150‑1 (MQ=255)
              cGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCTGCCTTTTGAtt                                                                                                                                         >  2:1299461/1‑150 (MQ=255)
              cGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTGTTGAtt                                                                                                                                         >  2:1808271/1‑150 (MQ=255)
              cGTCAACCGGTTTACCAACTCTACACGCAGTTAAATCGAGCAAGGTTCTTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCACGGAGCGGCCattatcct                                                                                                                                         <  1:690969/150‑6 (MQ=255)
              cGTCAACCGGTTTACCAACTATACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTTCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTTCTGCCTTTTGAta                                                                                                                                         >  1:957860/1‑149 (MQ=255)
               gTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATta                                                                                                                                        <  2:1898629/150‑1 (MQ=255)
                tCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATtaa                                                                                                                                       <  1:842576/150‑1 (MQ=255)
                 cAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGTGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATtaaa                                                                                                                                      >  2:492070/1‑150 (MQ=255)
                 cAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATtaaa                                                                                                                                      >  2:1497981/1‑150 (MQ=255)
                 cAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATtaaa                                                                                                                                      <  1:2001879/150‑1 (MQ=255)
                  aaCCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATtaaat                                                                                                                                     >  1:771834/1‑150 (MQ=255)
                  aaCCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATtaaat                                                                                                                                     <  2:844350/150‑1 (MQ=255)
                    ccGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATtaaataa                                                                                                                                   >  1:775470/1‑150 (MQ=255)
                      ggTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAAc                                                                                                                                 >  2:1091827/1‑150 (MQ=255)
                      ggTTTACCAACTCTACACGCTGTTAAATCGTGAAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGTATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGACTTTTGATtaaataaaa                                                                                                                                 >  1:23509/1‑149 (MQ=255)
                           aCCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAg                                                                                                                            <  2:1117284/150‑1 (MQ=255)
                            ccAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGc                                                                                                                           >  2:1962847/1‑150 (MQ=255)
                            ccAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGc                                                                                                                           >  2:1281841/1‑150 (MQ=255)
                               aCTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCATCATGATATGGGTTGAGGATAATGGCCGCTCCTTGCGGCCTTTTGATTAAATAAACCCAAGCagt                                                                                                                        >  1:268641/1‑149 (MQ=255)
                                atctACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATTGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCagag                                                                                                                       <  2:573266/149‑1 (MQ=255)
                                    acacGCTGATAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGaaaa                                                                                                                   <  1:509651/150‑1 (MQ=255)
                                     cacGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAg                                                                                                                  <  2:340889/150‑1 (MQ=255)
                                       cGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGaa                                                                                                                >  2:1750626/1‑150 (MQ=255)
                                       cGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGaa                                                                                                                <  1:837939/150‑1 (MQ=255)
                                         cTGTTAAATCGTGCTTGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAaca                                                                                                              <  2:1848855/150‑1 (MQ=255)
                                           gTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAAcaca                                                                                                            >  2:1848914/1‑150 (MQ=255)
                                           gTTAAATCGGGCAATGTTATTTGGCGGGCAGCATTTGGTTATTTCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATTGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAAcaca                                                                                                            <  2:1017631/150‑1 (MQ=255)
                                            ttAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTTCGGCCGTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACAt                                                                                                           >  1:983096/1‑150 (MQ=255)
                                                   gTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTgc                                                                                                    >  1:645234/1‑150 (MQ=255)
                                                   gTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTgc                                                                                                    >  2:475286/1‑150 (MQ=255)
                                                    tGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTgca                                                                                                   <  1:559372/150‑1 (MQ=255)
                                                    tGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTgca                                                                                                   >  2:238238/1‑150 (MQ=255)
                                                      cAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTgcagc                                                                                                 >  1:226001/1‑150 (MQ=255)
                                                      cAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACAAAACCTgcagc                                                                                                 >  2:250314/1‑150 (MQ=255)
                                                      cAAGGTTAGTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATACTGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTgcagc                                                                                                 <  2:1530741/150‑1 (MQ=255)
                                                          gTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATa                                                                                             <  2:1136970/150‑1 (MQ=255)
                                                           ttATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATAc                                                                                            <  1:1439502/150‑1 (MQ=255)
                                                              tttGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACaaa                                                                                         <  2:1734787/150‑1 (MQ=255)
                                                                tGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATa                                                                                       >  2:1002205/1‑150 (MQ=255)
                                                                       cAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCa                                                                                >  2:1107145/1‑150 (MQ=255)
                                                                       cAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCa                                                                                <  1:1402328/150‑1 (MQ=255)
                                                                         gCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAgg                                                                              <  2:1603248/150‑1 (MQ=255)
                                                                         gCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAgg                                                                              >  1:1120025/1‑150 (MQ=255)
                                                                          cATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGGTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGa                                                                             <  2:1737660/150‑1 (MQ=255)
                                                                          cATTTGGTTATTGCTCAGCAGTCATTCGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAACAACACATAACATGCAGCAATACAAATAACCGCCAgca                                                                             >  1:948424/1‑148 (MQ=255)
                                                                                gTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACa                                                                       <  1:1109511/150‑1 (MQ=255)
                                                                                 ttATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAg                                                                      >  1:544131/1‑150 (MQ=255)
                                                                                  tATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGt                                                                     <  1:1750626/150‑1 (MQ=255)
                                                                                   aTTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTg                                                                    >  2:904028/1‑150 (MQ=255)
                                                                                   aTTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTg                                                                    >  2:959761/1‑150 (MQ=255)
                                                                                   aTTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTg                                                                    <  1:800373/150‑1 (MQ=255)
                                                                                    ttGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTga                                                                   >  2:154772/1‑150 (MQ=255)
                                                                                     tGCTCATGAGTCATTGGCTAGATTATTATCAGCATTATATTGGTTGATGATAATGGCCGCGCCGTGCGGCCTTTTGATTAAAAAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTgag                                                                  <  1:1775279/150‑1 (MQ=255)
                                                                                       cTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGaa                                                                >  1:1319510/1‑150 (MQ=255)
                                                                                         cagcagTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGaaaa                                                              >  1:1943706/1‑150 (MQ=255)
                                                                                                cATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAg                                                       <  1:1564360/150‑1 (MQ=255)
                                                                                                  ttGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGa                                                     <  1:1299461/150‑1 (MQ=255)
                                                                                                  ttGGATAGATTATAAGCAGCATGATATGGGTTGAGGATACTGGCCGCTCCGTGCGGCCTTTTGGTTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGa                                                     <  1:1695359/150‑1 (MQ=255)
                                                                                                    ggATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGaaa                                                   >  2:568138/1‑150 (MQ=255)
                                                                                                      aTAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAAt                                                 <  2:1723210/150‑1 (MQ=255)
                                                                                                         gATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGt                                              >  2:1587069/1‑150 (MQ=255)
                                                                                                         gATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGt                                              >  2:402075/1‑150 (MQ=255)
                                                                                                         gATTATTAGCAGCATGATATGGGATGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAATAACACATAACCTGCAGAAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAAAAGt                                              >  1:128691/1‑150 (MQ=255)
                                                                                                          attattAGCAGCATGATATGGGTTGAGGATAATGGCCTCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTa                                             <  1:1046034/150‑1 (MQ=255)
                                                                                                                  cagcaTGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCgg                                     >  1:972548/1‑150 (MQ=255)
                                                                                                                    gcaTGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGaa                                   <  2:453809/150‑1 (MQ=255)
                                                                                                                     caTGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGaac                                  <  2:861874/150‑1 (MQ=255)
                                                                                                                        tatatGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGaacaac                               <  1:1659322/149‑1 (MQ=255)
                                                                                                                          tatGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAAcaacaa                             >  2:789958/1‑150 (MQ=255)
                                                                                                                           atGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAAt                            >  1:1721428/1‑150 (MQ=255)
                                                                                                                           atGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATAAAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAAt                            >  2:559180/1‑150 (MQ=255)
                                                                                                                               gTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACgg                        >  1:1362583/1‑150 (MQ=255)
                                                                                                                               gTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACgg                        >  2:876376/1‑150 (MQ=255)
                                                                                                                                  gatgATAATGTCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGAc                     <  1:1430959/147‑1 (MQ=255)
                                                                                                                                   aGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACAAAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACt                    >  1:1785693/1‑150 (MQ=255)
                                                                                                                                    ggATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTc                   >  1:43779/1‑150 (MQ=255)
                                                                                                                                       tAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGACCACATAACCTGAAGCAATACAAATACCAGGCAGGAGATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTCCGAAACAAGAATACGGTACTAAAt                >  1:288133/1‑150 (MQ=255)
                                                                                                                                       aaaaggCCGCACGGAGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATTCTGGACTCatt                >  1:305289/5‑148 (MQ=255)
                                                                                                                                        aaTGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATACCCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAAtt               <  2:1069223/150‑1 (MQ=255)
                                                                                                                                         aTGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAAttt              >  1:1283644/1‑150 (MQ=255)
                                                                                                                                                cTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCAcg       <  1:1745072/150‑1 (MQ=255)
                                                                                                                                                  ccGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAAGACATAACCTGCAGCAATACAAATAAACGGCAGGATATACAGTGAGAAAAACTGCAGGATAATACTAAGGTGCGGAACAACAATACGGGACTCAATTTGTTCAcgag     >  1:125242/1‑148 (MQ=255)
                                                                                                                                                    gTGCGTCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGtt   <  2:915419/150‑1 (MQ=255)
                                                                                                                                                     tGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTa  <  2:30370/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCGATTTCCTTGAACGTCAACCGGTTTACCAACTCTACACGCTGTTAAATCGTGCAAGGTTATTTGGCGGTCAGCATTTGGTTATTGCTCAGCAGTCATTGGATAGATTATTAGCAGCATGATATGGGTTGAGGATAATGGCCGCTCCGTGCGGCCTTTTGATTAAATAAACCCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTA  >  NC_000913/1808535‑1808833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: