Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,810,069 G→A 7.2% intergenic (+21/‑142) yniC → / → ydjM 2‑deoxyglucose‑6‑P phosphatase/inner membrane protein regulated by LexA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,810,0690GA 7.2% 508.0 / 17.4 165intergenic (+21/‑142)yniC/ydjM2‑deoxyglucose‑6‑P phosphatase/inner membrane protein regulated by LexA
Reads supporting (aligned to +/- strand):  ref base G (71/82);  new base A (5/7);  total (76/89)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-01

Only 100 of 166 total aligned reads displayed.

TCTAAAGCAGCCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTGAGTTTTATCATGACGG  >  NC_000913/1809920‑1810217
                                                                                                                                                     |                                                                                                                                                    
tctAAAGCAGCCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGCCCTTCTCGGTTAATGACCAGGGGCAGTGATCTCg                                                                                                                                                      >  1:1208845/1‑150 (MQ=255)
 ctAAAGCAGCCCGCATGCGTTCCTTCGTCGTTCCTGCGCCAGAAGCTCAAATTGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGc                                                                                                                                                     <  2:72522/150‑1 (MQ=255)
 ctAAAGCAGCCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGc                                                                                                                                                     <  1:1565231/150‑1 (MQ=255)
  tAAAGCAGCCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCt                                                                                                                                                    <  1:861410/150‑1 (MQ=255)
    aaGCAGCCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGc                                                                                                                                                  >  1:990966/1‑150 (MQ=255)
     agcagcCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGCGAGATCACTGcc                                                                                                                                                 <  1:1326850/150‑1 (MQ=255)
       cagcCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGcccc                                                                                                                                               <  2:1349222/150‑1 (MQ=255)
       cagcCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGcccc                                                                                                                                               <  2:1231100/150‑1 (MQ=255)
       cagcCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGCGAGATCACTGcccc                                                                                                                                               <  1:1089041/150‑1 (MQ=255)
       cagcCCGCAGGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGCGAGATCACTGcccc                                                                                                                                               <  1:269774/150‑1 (MQ=255)
         gcCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTg                                                                                                                                             <  1:581817/150‑1 (MQ=255)
         gcCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTg                                                                                                                                             >  2:1279904/1‑150 (MQ=255)
         gcCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGCGAGATCACTGCCCCTg                                                                                                                                             <  1:1029596/150‑1 (MQ=255)
          cccGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGCGAGATCACTGCCCCTgg                                                                                                                                            <  2:791270/150‑1 (MQ=255)
           ccGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGCACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGt                                                                                                                                           <  2:723280/150‑1 (MQ=255)
            cGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGtt                                                                                                                                          >  1:910866/1‑150 (MQ=255)
            cGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGtt                                                                                                                                          >  2:1747009/1‑150 (MQ=255)
               aTGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCtt                                                                                                                                       <  2:1208467/150‑1 (MQ=255)
                tGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCttt                                                                                                                                      <  2:397541/150‑1 (MQ=255)
                 gCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTa                                                                                                                                     <  1:774292/150‑1 (MQ=255)
                 gCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTa                                                                                                                                     <  1:1443052/150‑1 (MQ=255)
                    ttCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCt                                                                                                                                  <  1:1245335/150‑1 (MQ=255)
                        aTCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGaaa                                                                                                                              <  2:30465/150‑1 (MQ=255)
                         tcgtcgTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAAt                                                                                                                             <  2:91883/150‑1 (MQ=255)
                              gtTACTGCGCCAGATGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAAGTTTCATCGCTGACAGAACTCACAGCAAACGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCAt                                                                                                                        <  1:1518330/150‑1 (MQ=255)
                               ttCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCAtt                                                                                                                       >  1:1363322/1‑150 (MQ=255)
                                   tGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAAc                                                                                                                   >  1:1403465/1‑150 (MQ=255)
                                   tGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAAc                                                                                                                   <  2:741675/150‑1 (MQ=255)
                                     cgcCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTg                                                                                                                 >  1:504489/1‑150 (MQ=255)
                                       ccAGAAGCTCAAAATGATCCACGTTTTGTATTAGCAGACTTCAAACTTTCATCGCTGACAGAACTAACCTCAAAAGACCGTCTAGTTTAATGACAAGGGGCATTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGAc                                                                                                               <  1:1639316/150‑1 (MQ=255)
                                        cAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACg                                                                                                              <  2:1760779/150‑1 (MQ=255)
                                               gcAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAAt                                                                                                       >  1:1494736/1‑150 (MQ=255)
                                               gcAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAAt                                                                                                       >  1:2018403/1‑150 (MQ=255)
                                               gcAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACATTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAAt                                                                                                       <  1:1847571/150‑1 (MQ=255)
                                                cAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGATCTCACCTCAAAAGACCTTCTCGTTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATc                                                                                                      <  2:333755/150‑1 (MQ=255)
                                                   aaTGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTc                                                                                                   <  2:403659/150‑1 (MQ=255)
                                                    aTGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCa                                                                                                  <  1:1588411/150‑1 (MQ=255)
                                                      gATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAAt                                                                                                >  1:45883/1‑150 (MQ=255)
                                                        tCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAAttt                                                                                              >  1:976429/1‑150 (MQ=255)
                                                           aCGTTTTGTATTAGCAGACTTCAAACTTTCATCGCTGACAGAACTGACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAag                                                                                           <  1:659570/150‑1 (MQ=255)
                                                           aCGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAag                                                                                           <  1:753158/150‑1 (MQ=255)
                                                            gtttttGTATTAGCAGACGTGAAACATTCTTCGCTGACAGTACTCACCGCAATTGACCTTCTTGGTTAATGTCCAGGGGCAGTGATTTCGCTGCACCTGGTTCTTTATCTGAAATTGCTTTCAACTGACGGATTAATTGTCTATTTAaga                                                                                          <  1:284808/148‑1 (MQ=255)
                                                            cGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAaga                                                                                          <  1:1589167/150‑1 (MQ=255)
                                                             gTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAagag                                                                                         <  2:1208845/150‑1 (MQ=255)
                                                               tttGTATTATCAGACGTCATCCTTTCCTCGGTGTCAGAAGTCACCAGAAAAGACCATCTCGGGTAATGACCAGGCGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCCTTCAAGTGACGGATTAATCTTCTATTTAAGAGaa                                                                                       <  2:1156357/150‑1 (MQ=255)
                                                                 tGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAag                                                                                     <  2:860234/150‑1 (MQ=255)
                                                                    aTTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGt                                                                                  >  2:1109762/1‑150 (MQ=255)
                                                                    aTTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGt                                                                                  >  1:1893905/1‑150 (MQ=255)
                                                                     ttAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGtt                                                                                 <  2:227039/150‑1 (MQ=255)
                                                                     ttAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACAAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGtt                                                                                 <  1:169143/150‑1 (MQ=255)
                                                                       aGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTac                                                                               >  2:1296771/1‑150 (MQ=255)
                                                                             cGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGAAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTc                                                                         <  2:201960/150‑1 (MQ=255)
                                                                                cAAACTTTCATCGCTGACAGAACTCACCGCAAAAGCCCTTCTCGGATAATGACCAGGGGCAGTGATCTAGCAGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGAAGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTcacc                                                                      >  1:1544077/1‑150 (MQ=255)
                                                                                cAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGTCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTcacc                                                                      <  1:1087406/150‑1 (MQ=255)
                                                                                cAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGCCGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTcacc                                                                      >  1:1709956/1‑150 (MQ=255)
                                                                                   aCTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACt                                                                   <  1:1176914/150‑1 (MQ=255)
                                                                                         aTCTCTGCCAGACCACACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTg                                                             <  1:1870848/150‑1 (MQ=255)
                                                                                          acGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTGATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGc                                                            <  2:1328059/149‑1 (MQ=255)
                                                                                             cTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACt                                                         >  1:436831/1‑150 (MQ=255)
                                                                                             cTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACt                                                         <  1:595454/150‑1 (MQ=255)
                                                                                                    aaCTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATaaa                                                  <  1:261721/150‑1 (MQ=255)
                                                                                                     aCTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATaaaa                                                 <  1:50134/150‑1 (MQ=255)
                                                                                                      cTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATaaaca                                                >  2:1890827/1‑148 (MQ=255)
                                                                                                      cTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAACAGTTACACCGTCACCACTTCCTTGCACTGTATaaaaa                                                >  2:929336/1‑150 (MQ=255)
                                                                                                        cACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATc                                              >  1:1245604/1‑150 (MQ=255)
                                                                                                        cACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATc                                              >  2:444574/1‑150 (MQ=255)
                                                                                                         aCCGCCAAAGACCTTCTCGGTTAATGACCAGGTGCAGAGATCTCGCGGCCCCTGGTTCTTTATCTGAAATTGCACTCAACTGACGGATTAATCGTCAATTTAAGAGAACGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATcc                                             <  1:85352/150‑1 (MQ=255)
                                                                                                          ccGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCt                                            >  1:877159/1‑150 (MQ=255)
                                                                                                          ccGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCt                                            >  1:2001539/1‑150 (MQ=255)
                                                                                                          ccGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCt                                            >  1:738474/1‑150 (MQ=255)
                                                                                                          ccGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCt                                            <  1:1074469/150‑1 (MQ=255)
                                                                                                           cGCAAAAGACCTTCTCGGTTAATGAGCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCta                                           <  2:993095/150‑1 (MQ=255)
                                                                                                           cGCAAAACACCTTCTCGGTTAATGACCATGGGCAGTGCTCTCGCTTCCCCTTGTTCTTTATCTGAAATTGCATTCACCTGACGGATTAATCGTCAATTTAAGAGAAAGAGATACCCCGGCACCACTTCCGTGCACTGTATAAAAATCCta                                           <  2:302338/150‑1 (MQ=255)
                                                                                                            gCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCtat                                          <  1:1216878/150‑1 (MQ=255)
                                                                                                             cAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCtata                                         >  2:1680847/1‑150 (MQ=255)
                                                                                                              aaaaGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATAc                                        <  1:1557875/150‑1 (MQ=255)
                                                                                                              aaaaGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATAc                                        <  2:1085730/150‑1 (MQ=255)
                                                                                                                  gACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTa                                    >  1:1916253/1‑150 (MQ=255)
                                                                                                                           ggTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCAGTGTATAAAAATCCTATACTGTACGTATCGAc                           >  1:547673/1‑150 (MQ=255)
                                                                                                                                   aCCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGt                   >  2:1386850/1‑150 (MQ=255)
                                                                                                                                    ccAGGGTCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTg                  <  2:1499001/150‑1 (MQ=255)
                                                                                                                                    ccAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCCCCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTg                  >  2:1593037/1‑150 (MQ=255)
                                                                                                                                    ccAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTg                  >  2:101853/1‑150 (MQ=255)
                                                                                                                                    ccAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTg                  <  2:520294/150‑1 (MQ=255)
                                                                                                                                    ccAGGGGCAGCGAGATCACTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTg                  >  1:431605/1‑150 (MQ=255)
                                                                                                                                     cAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTGa                 >  1:1628572/1‑150 (MQ=255)
                                                                                                                                     cAGGGGCAGCGAGATCACTGCCCCTGGTTCTTTATCTGAAATTGCATTCTACTGACGGATTAATCGGCAATTTAAGAGAAAGAGTAACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTgg                 >  1:127349/1‑149 (MQ=255)
                                                                                                                                      aGGGGCAGCGAGATCACTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGTATAAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACAACTTCCGTGCACAGTATAAAAATCCTATACTGTACGTATCGACAGGTTTGTCAg                >  2:77577/1‑150 (MQ=255)
                                                                                                                                       ggggCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTGAGt               >  2:591504/1‑150 (MQ=255)
                                                                                                                                        gggCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTGAGtt              >  2:1461850/1‑150 (MQ=255)
                                                                                                                                         ggCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTGAGttt             <  1:1757056/150‑1 (MQ=255)
                                                                                                                                          gCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTGAGtttt            <  1:1747009/150‑1 (MQ=255)
                                                                                                                                            aGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTGAGTTTTAt          <  2:1472824/150‑1 (MQ=255)
                                                                                                                                             gTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTGAGTTTTATc         <  2:144335/150‑1 (MQ=255)
                                                                                                                                             gTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCAATCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTGAGTTTTATc         <  2:1461057/150‑1 (MQ=255)
                                                                                                                                              tGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTGAGTTTTATCa        >  1:849243/1‑150 (MQ=255)
                                                                                                                                               gATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTGAGTTGTATcct       >  1:305008/1‑148 (MQ=255)
                                                                                                                                               agtctcGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCATCTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACAGTAACCACGTCCGTGCACTGTATAAATATCCTAAACTGTACGTATCGACAGTTTAGTGAGTTTTATCAt       >  2:1833541/3‑150 (MQ=255)
                                                                                                                                                   tcGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATAGTCAATTTAAGAGAAAGAGTTACCCCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTGAGTTTTATCATGAcg   >  1:1123383/1‑150 (MQ=255)
                                                                                                                                                    cGCTGCCCCTGGTTCTGTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCAATTCAGTGCACTGTATAAAAATCCTATACTGTCCGTTTCGACAGGTTAGTGAGGTTTATCTTGAcgg  >  2:300961/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TCTAAAGCAGCCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTGAGTTTTATCATGACGG  >  NC_000913/1809920‑1810217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: