Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,825,936 T→A 6.3% intergenic (‑311/+19) spy ← / ← astE periplasmic ATP‑independent protein refolding chaperone, stress‑induced/succinylglutamate desuccinylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,825,9360TA 6.3% 312.2 / 7.0 105intergenic (‑311/+19)spy/astEperiplasmic ATP‑independent protein refolding chaperone, stress‑induced/succinylglutamate desuccinylase
Reads supporting (aligned to +/- strand):  ref base T (54/44);  new base A (3/4);  total (57/48)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

ATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGTTCCTT  >  NC_000913/1825788‑1826085
                                                                                                                                                    |                                                                                                                                                     
aTTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGATGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCACCTTAATACCCGCAGAATg                                                                                                                                                      >  2:338309/1‑150 (MQ=255)
aTTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATg                                                                                                                                                      >  2:656710/1‑150 (MQ=255)
aTTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATg                                                                                                                                                      <  1:1680526/150‑1 (MQ=255)
 tttGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGa                                                                                                                                                     >  2:94451/1‑150 (MQ=255)
     aTGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTc                                                                                                                                                 <  2:991480/150‑1 (MQ=255)
         agagTGTTGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGACCTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCg                                                                                                                                             <  2:1093612/150‑1 (MQ=255)
         agagTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCg                                                                                                                                             <  1:49212/150‑1 (MQ=255)
          gagTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAAATCATTCTGCgg                                                                                                                                            <  1:1501600/150‑1 (MQ=255)
          gagTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAAATCATTCTGCgg                                                                                                                                            <  1:563722/150‑1 (MQ=255)
          gagTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAAATCATTCTGCgg                                                                                                                                            <  1:556008/150‑1 (MQ=255)
              gatgaGATAATATTTCTTCACGATGATTTTGCAGAGATTATGAAGAACAATACCGGATGACTGGTGATAACTAAAGCAAATAACCAGGATTAATCTGTAGTAATTTATAAGAAAGCAACTAAATACACGCAGAAATCATTCTGCGGGTat                                                                                                                                        <  2:320602/150‑2 (MQ=255)
                tgaGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGt                                                                                                                                      <  2:438099/150‑1 (MQ=255)
                 gagagaATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTa                                                                                                                                     <  2:616760/150‑1 (MQ=255)
                  agagaATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTAt                                                                                                                                    >  1:1875874/1‑150 (MQ=255)
                      aaTATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGc                                                                                                                                <  1:842383/150‑1 (MQ=255)
                       atatTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCt                                                                                                                               <  1:1052728/150‑1 (MQ=255)
                          ttACAACACGATGATTTTGCAGAGATTATGTAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTAt                                                                                                                            >  1:1247062/1‑150 (MQ=255)
                            acaacaCGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTAttt                                                                                                                          >  2:1554634/1‑150 (MQ=255)
                            acaacaCGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTAttt                                                                                                                          <  1:510237/150‑1 (MQ=255)
                             caacaCGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAATCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTAtttt                                                                                                                         >  1:1083237/1‑150 (MQ=255)
                                cacGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTc                                                                                                                      <  1:1685338/150‑1 (MQ=255)
                                 acGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCg                                                                                                                     <  2:256935/150‑1 (MQ=255)
                                  cGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGa                                                                                                                    >  1:1793091/1‑150 (MQ=255)
                                   gatgatTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAg                                                                                                                   <  2:760739/150‑1 (MQ=255)
                                    atgatTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGc                                                                                                                  <  2:1388658/150‑1 (MQ=255)
                                      gatTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCAt                                                                                                                <  1:1376682/150‑1 (MQ=255)
                                        ttttGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTa                                                                                                              >  1:1224791/1‑150 (MQ=255)
                                           tGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGATTATTTTTTCTAGCATTAAta                                                                                                           >  1:1760345/1‑149 (MQ=255)
                                               gagaTTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCcgc                                                                                                       >  1:2614/1‑150 (MQ=255)
                                               gagaTTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGAAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCcgc                                                                                                       >  2:723333/1‑150 (MQ=255)
                                                ataTTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCcgcg                                                                                                      <  2:158978/148‑1 (MQ=255)
                                                agaTTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCcgcg                                                                                                      >  1:485842/1‑150 (MQ=255)
                                                       gaagaaCTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGTGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATc                                                                                               >  2:1646208/1‑150 (MQ=255)
                                                       gaagaaCTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACACGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTCTTTCGAGCATTAATCCCGCGCGTAATc                                                                                               >  2:1658535/1‑150 (MQ=255)
                                                        aagaaCTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATcc                                                                                              <  2:1773249/150‑1 (MQ=255)
                                                           aaCTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCaa                                                                                           <  2:1289921/150‑1 (MQ=255)
                                                            aCTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAAc                                                                                          >  1:678907/1‑150 (MQ=255)
                                                            aCTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAAc                                                                                          <  2:453568/150‑1 (MQ=255)
                                                             atataCCGTATGACTGGTGATAAATAAATCAAATAACCAGTATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACg                                                                                         <  2:588544/149‑1 (MQ=255)
                                                              tataCCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCTAGCATTAATCCCGCGCGTAATCCCAACGc                                                                                        >  1:1202445/1‑150 (MQ=255)
                                                              tataCCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGc                                                                                        >  1:1956646/1‑150 (MQ=255)
                                                              tataCCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGc                                                                                        <  2:788219/150‑1 (MQ=255)
                                                               ataCCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCt                                                                                       >  2:753893/1‑150 (MQ=255)
                                                               ataCCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCt                                                                                       >  2:336938/1‑150 (MQ=255)
                                                               ataCCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCt                                                                                       <  2:516464/150‑1 (MQ=255)
                                                               ataCCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCt                                                                                       >  2:1119359/1‑150 (MQ=255)
                                                                taactgATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGAGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTa                                                                                      <  2:958013/145‑1 (MQ=255)
                                                                  ccGGATGACTTGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCTGTTAATTATTAGCTAATTTTTTAGAGCATTAATCACTCGCGTAATCCCAACTCTAcc                                                                                    >  1:987756/1‑150 (MQ=255)
                                                                      aTGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACg                                                                                <  2:283079/150‑1 (MQ=255)
                                                                       tGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACgg                                                                               <  2:1868819/150‑1 (MQ=255)
                                                                       tGACTGGAGATAAATAAAGCAAATAACCAGGATTAAGCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGACTGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTACTCCCAACGCTACCAACgg                                                                               <  2:1384903/150‑1 (MQ=255)
                                                                         aCTGGTGATAAATAAAGCTAATAACCAGGATTAATCTGTATTAATTTTTAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTATCTTATTTTTTCGACCATTAATCCAGCGCGTAAACCCAACGCTACCAACGGAt                                                                             >  1:233126/1‑150 (MQ=255)
                                                                            ggTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAg                                                                          <  1:1767912/150‑1 (MQ=255)
                                                                            ggTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAg                                                                          >  1:1697948/1‑150 (MQ=255)
                                                                            ggTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGAGTGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAAAGGATTAg                                                                          >  1:40767/1‑150 (MQ=255)
                                                                             gTGATAAATAAAGCATATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGAGTAATCCCAACGCTACCAACGGATTAgg                                                                         <  2:1818123/150‑1 (MQ=255)
                                                                              tGATAAATAAAGCAACTAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAggg                                                                        <  2:925357/150‑1 (MQ=255)
                                                                               gATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGa                                                                       <  2:204277/150‑1 (MQ=255)
                                                                                 taaataaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAAt                                                                     <  1:1479204/150‑1 (MQ=255)
                                                                                 taaataaaGCAAATAACCAGGATTAATCTGAATTAATTTATAAGAAAGCAACTTAATAACCGCAGAATGATTACTGCGGGTAAGTATTAGATTATTTTTTACAGAATTAATCACTCGCGTAATCACAAAGCTACAAACGGATTAGGGAAt                                                                     >  1:1434012/1‑150 (MQ=255)
                                                                                    ataaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAac                                                                  <  1:1874843/150‑1 (MQ=255)
                                                                                      aaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACAAACGGATTAGGGAATAacaa                                                                >  2:329487/1‑149 (MQ=255)
                                                                                         gCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGATTATATTTTCGAGCATTAATCCCGCTCGTAATCCAAACTCTACAAACGTATTAGGGAATAACACATa                                                             >  1:109413/1‑150 (MQ=255)
                                                                                          cAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGAGTGTAATTATTAGCTTATTTTATAGAGCATTAATCCCGCTCGTAATCCCAACGCTACAAACGGATTAGGGAATAACACATAc                                                            >  1:185818/1‑150 (MQ=255)
                                                                                           aaaTAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATAct                                                           <  1:25871/150‑1 (MQ=255)
                                                                                               aaCCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTAc                                                       >  1:275248/1‑150 (MQ=255)
                                                                                               aaCCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATATCTGCGGGTAAGTATTAGCTTATTTTATCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTAc                                                       <  2:1154806/150‑1 (MQ=255)
                                                                                                aCCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATACCGCGCGTAATCCCAACGCTACCAACTGATAAGGGAAAAACACATACTCTAcc                                                      >  2:197685/1‑149 (MQ=255)
                                                                                                  cAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTAcatc                                                    <  2:817703/150‑1 (MQ=255)
                                                                                                       ttAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATggg                                               <  1:168496/150‑1 (MQ=255)
                                                                                                         aaTCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGtt                                             >  2:1832620/1‑150 (MQ=255)
                                                                                                          aTCTGTATTAATTTATTAGAATGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGCTTa                                            >  1:306152/1‑150 (MQ=255)
                                                                                                           tCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTAc                                           <  1:989119/150‑1 (MQ=255)
                                                                                                              gTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGt                                        >  2:1369451/1‑150 (MQ=255)
                                                                                                               tATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATCGGTTACGGTa                                       >  2:1117541/1‑150 (MQ=255)
                                                                                                                 ttAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCATCGGATTAGGGATTAACACATACTCTACATCATGGGTTACGGTaaa                                     >  2:1791615/1‑150 (MQ=255)
                                                                                                                    aTTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGATTAACACATACTCTACATCATGGGTTACGGTAAAACg                                  >  2:1087183/1‑150 (MQ=255)
                                                                                                                      ttATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGtt                                >  2:1060270/1‑150 (MQ=255)
                                                                                                                       tataAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTc                               >  1:1374599/1‑150 (MQ=255)
                                                                                                                        ataAGAAAGCAACTTAATTCCCGCAGAAAGATTTGTGCGGGTAAGTATTAGCTTAATTTTTCTAGAATTAATCAAGCGAGTAATCCAAACGATACCTACGGATTAGGGAATATCACATTCTCTAGATCATGAGATACGGTATAACGTTcc                              >  1:313870/1‑150 (MQ=255)
                                                                                                                        ataAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTcc                              <  1:1269972/150‑1 (MQ=255)
                                                                                                                         taAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTCCCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCct                             >  1:1230484/1‑150 (MQ=255)
                                                                                                                         taAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCct                             >  2:1890281/1‑150 (MQ=255)
                                                                                                                         taAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCct                             >  2:1584102/1‑150 (MQ=255)
                                                                                                                          aaGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCctc                            >  2:756980/1‑150 (MQ=255)
                                                                                                                             aaaGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATATTTTCGAGCATTAATACCGCGCGTAATCCCAACGCTTCCAACGGATTAGGGAATAACACAAACTCTACATCATGGTTTACGGTAAAACGTTCCTCTcc                         >  2:1950742/1‑150 (MQ=255)
                                                                                                                               aGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATCACCCATACTCTACTTCAGGGGTTACGGTAAAACGTTCCTCTCCgg                       >  2:137886/1‑149 (MQ=255)
                                                                                                                               aGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGt                       <  1:1978178/150‑1 (MQ=255)
                                                                                                                                   aCTTAATACCCGCCCAATGATTTCTGCGTGTAAGTATTAGCTTATTTTTTCGAGCATAAATACAGCGCTTAATCCCAACGCTACCAACGGATTAGGGAATAACAAATACTCTACATAATGGGTTACGGTAAAACGTTCCTCTCCGTCCTg                   <  2:83092/150‑1 (MQ=255)
                                                                                                                                   aCTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTg                   >  2:1680712/1‑150 (MQ=255)
                                                                                                                                   aCTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTg                   >  1:1934614/1‑150 (MQ=255)
                                                                                                                                       aaTACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGcc               >  2:671758/1‑150 (MQ=255)
                                                                                                                                        aTACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCa              <  1:1565924/150‑1 (MQ=255)
                                                                                                                                           cccGCAGAAATCATTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCa           >  1:582409/1‑150 (MQ=255)
                                                                                                                                           cccGCAGAAATCATTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCa           >  1:1306917/1‑150 (MQ=255)
                                                                                                                                              gCAGTATGTTTTCTTCGTGTAAGTATTAGCTTATTTTTTCGATCATTAATCCCGCGCGTTATCGCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATg        <  2:925908/150‑1 (MQ=255)
                                                                                                                                                aGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTAGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGTAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGtt      <  2:1355519/150‑1 (MQ=255)
                                                                                                                                                 gAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGCATTAGGGAATACCACATACTCGACTTCAGGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGATc     >  2:956897/1‑150 (MQ=255)
                                                                                                                                                    tGATTTGTGGGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGTTCCtt  <  2:185818/150‑1 (MQ=255)
                                                                                                                                                    tGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGTTCCtt  <  1:1601859/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGTTCCTT  >  NC_000913/1825788‑1826085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: