Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,825,938 A→C 5.8% intergenic (‑313/+17) spy ← / ← astE periplasmic ATP‑independent protein refolding chaperone, stress‑induced/succinylglutamate desuccinylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,825,9380AC 5.8% 317.4 / 8.4 104intergenic (‑313/+17)spy/astEperiplasmic ATP‑independent protein refolding chaperone, stress‑induced/succinylglutamate desuccinylase
Reads supporting (aligned to +/- strand):  ref base A (52/45);  new base C (2/4);  total (54/50)
Fisher's exact test for biased strand distribution p-value = 4.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.72e-01

Only 100 of 107 total aligned reads displayed.

TTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGTTCCTTTC  >  NC_000913/1825789‑1826087
                                                                                                                                                     |                                                                                                                                                     
tttGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGa                                                                                                                                                       >  2:94451/1‑150 (MQ=255)
    aTGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTc                                                                                                                                                   <  2:991480/150‑1 (MQ=255)
        agagTGTTGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGACCTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCg                                                                                                                                               <  2:1093612/150‑1 (MQ=255)
        agagTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCg                                                                                                                                               <  1:49212/150‑1 (MQ=255)
         gagTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAAATCATTCTGCgg                                                                                                                                              <  1:556008/150‑1 (MQ=255)
         gagTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAAATCATTCTGCgg                                                                                                                                              <  1:1501600/150‑1 (MQ=255)
         gagTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAAATCATTCTGCgg                                                                                                                                              <  1:563722/150‑1 (MQ=255)
             gatgaGATAATATTTCTTCACGATGATTTTGCAGAGATTATGAAGAACAATACCGGATGACTGGTGATAACTAAAGCAAATAACCAGGATTAATCTGTAGTAATTTATAAGAAAGCAACTAAATACACGCAGAAATCATTCTGCGGGTat                                                                                                                                          <  2:320602/150‑2 (MQ=255)
               tgaGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGt                                                                                                                                        <  2:438099/150‑1 (MQ=255)
                gagagaATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTa                                                                                                                                       <  2:616760/150‑1 (MQ=255)
                 agagaATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTAt                                                                                                                                      >  1:1875874/1‑150 (MQ=255)
                     aaTATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGc                                                                                                                                  <  1:842383/150‑1 (MQ=255)
                      atatTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCt                                                                                                                                 <  1:1052728/150‑1 (MQ=255)
                         ttACAACACGATGATTTTGCAGAGATTATGTAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTAt                                                                                                                              >  1:1247062/1‑150 (MQ=255)
                           acaacaCGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTAttt                                                                                                                            <  1:510237/150‑1 (MQ=255)
                           acaacaCGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTAttt                                                                                                                            >  2:1554634/1‑150 (MQ=255)
                            caacaCGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAATCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTAtttt                                                                                                                           >  1:1083237/1‑150 (MQ=255)
                               cacGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTc                                                                                                                        <  1:1685338/150‑1 (MQ=255)
                                acGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCg                                                                                                                       <  1:758383/150‑1 (MQ=255)
                                acGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCg                                                                                                                       <  2:256935/150‑1 (MQ=255)
                                 cGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGa                                                                                                                      >  1:1793091/1‑150 (MQ=255)
                                  gatgatTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAg                                                                                                                     <  2:760739/150‑1 (MQ=255)
                                   atgatTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGc                                                                                                                    <  2:1388658/150‑1 (MQ=255)
                                     gatTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCAt                                                                                                                  <  1:1376682/150‑1 (MQ=255)
                                       ttttGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTa                                                                                                                >  1:1224791/1‑150 (MQ=255)
                                          tGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGATTATTTTTTCTAGCATTAAta                                                                                                             >  1:1760345/1‑149 (MQ=255)
                                              gagaTTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCcgc                                                                                                         >  1:2614/1‑150 (MQ=255)
                                              gagaTTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGAAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCcgc                                                                                                         >  2:723333/1‑150 (MQ=255)
                                               ataTTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCcgcg                                                                                                        <  2:158978/148‑1 (MQ=255)
                                               agaTTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCcgcg                                                                                                        >  1:485842/1‑150 (MQ=255)
                                                      gaagaaCTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGTGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATc                                                                                                 >  2:1646208/1‑150 (MQ=255)
                                                      gaagaaCTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACACGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTCTTTCGAGCATTAATCCCGCGCGTAATc                                                                                                 >  2:1658535/1‑150 (MQ=255)
                                                       aagaaCTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATcc                                                                                                <  2:1773249/150‑1 (MQ=255)
                                                          aaCTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCaa                                                                                             <  2:1289921/150‑1 (MQ=255)
                                                           aCTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAAc                                                                                            <  2:453568/150‑1 (MQ=255)
                                                            atataCCGTATGACTGGTGATAAATAAATCAAATAACCAGTATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACg                                                                                           <  2:588544/149‑1 (MQ=255)
                                                             tataCCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCTAGCATTAATCCCGCGCGTAATCCCAACGc                                                                                          >  1:1202445/1‑150 (MQ=255)
                                                             tataCCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGc                                                                                          >  1:1956646/1‑150 (MQ=255)
                                                             tataCCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGc                                                                                          <  2:788219/150‑1 (MQ=255)
                                                              ataCCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCt                                                                                         >  2:1119359/1‑150 (MQ=255)
                                                              ataCCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCt                                                                                         >  2:336938/1‑150 (MQ=255)
                                                              ataCCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCt                                                                                         <  2:516464/150‑1 (MQ=255)
                                                              ataCCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCt                                                                                         >  2:753893/1‑150 (MQ=255)
                                                               taactgATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGAGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTa                                                                                        <  2:958013/145‑1 (MQ=255)
                                                                 ccGGATGACTTGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCTGTTAATTATTAGCTAATTTTTTAGAGCATTAATCACTCGCGTAATCCCAACTCTAcc                                                                                      >  1:987756/1‑150 (MQ=255)
                                                                     aTGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACg                                                                                  <  2:283079/150‑1 (MQ=255)
                                                                      tGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACgg                                                                                 <  2:1868819/150‑1 (MQ=255)
                                                                           ggTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAg                                                                            >  1:1697948/1‑150 (MQ=255)
                                                                           ggTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAg                                                                            <  1:1767912/150‑1 (MQ=255)
                                                                           ggTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGAGTGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAAAGGATTAg                                                                            >  1:40767/1‑150 (MQ=255)
                                                                            gTGATAAATAAAGCATATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGAGTAATCCCAACGCTACCAACGGATTAgg                                                                           <  2:1818123/150‑1 (MQ=255)
                                                                             tGATAAATAAAGCAACTAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAggg                                                                          <  2:925357/150‑1 (MQ=255)
                                                                              gATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGa                                                                         <  2:204277/150‑1 (MQ=255)
                                                                                taaataaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAAt                                                                       <  1:1479204/150‑1 (MQ=255)
                                                                                taaataaaGCAAATAACCAGGATTAATCTGAATTAATTTATAAGAAAGCAACTTAATAACCGCAGAATGATTACTGCGGGTAAGTATTAGATTATTTTTTACAGAATTAATCACTCGCGTAATCACAAAGCTACAAACGGATTAGGGAAt                                                                       >  1:1434012/1‑150 (MQ=255)
                                                                                  aataaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCACCGGATTAGGGAATaa                                                                     >  2:1419396/1‑150 (MQ=255)
                                                                                   ataaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAac                                                                    <  1:1874843/150‑1 (MQ=255)
                                                                                     aaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACAAACGGATTAGGGAATAacaa                                                                  >  2:329487/1‑149 (MQ=255)
                                                                                       aGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACAt                                                                >  2:1957777/1‑150 (MQ=255)
                                                                                        gCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGATTATATTTTCGAGCATTAATCCCGCTCGTAATCCAAACTCTACAAACGTATTAGGGAATAACACATa                                                               >  1:109413/1‑150 (MQ=255)
                                                                                         cAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGAGTGTAATTATTAGCTTATTTTATAGAGCATTAATCCCGCTCGTAATCCCAACGCTACAAACGGATTAGGGAATAACACATAc                                                              >  1:185818/1‑150 (MQ=255)
                                                                                          aaaTAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATAct                                                             <  1:25871/150‑1 (MQ=255)
                                                                                              aaCCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTAc                                                         >  1:275248/1‑150 (MQ=255)
                                                                                              aaCCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATATCTGCGGGTAAGTATTAGCTTATTTTATCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTAc                                                         <  2:1154806/150‑1 (MQ=255)
                                                                                               aCCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATACCGCGCGTAATCCCAACGCTACCAACTGATAAGGGAAAAACACATACTCTAcc                                                        >  2:197685/1‑149 (MQ=255)
                                                                                                 cAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTAcatc                                                      <  2:817703/150‑1 (MQ=255)
                                                                                                      ttAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATggg                                                 <  1:168496/150‑1 (MQ=255)
                                                                                                        aaTCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGtt                                               >  2:1832620/1‑150 (MQ=255)
                                                                                                         aTCTGTATTAATTTATTAGAATGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGCTTa                                              >  1:306152/1‑150 (MQ=255)
                                                                                                          tCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTAc                                             <  1:989119/150‑1 (MQ=255)
                                                                                                             gTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGt                                          >  2:1369451/1‑150 (MQ=255)
                                                                                                              tATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATCGGTTACGGTa                                         >  2:1117541/1‑150 (MQ=255)
                                                                                                                ttAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCATCGGATTAGGGATTAACACATACTCTACATCATGGGTTACGGTaaa                                       >  2:1791615/1‑150 (MQ=255)
                                                                                                                   aTTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGATTAACACATACTCTACATCATGGGTTACGGTAAAACg                                    >  2:1087183/1‑150 (MQ=255)
                                                                                                                     ttATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGtt                                  >  2:1060270/1‑150 (MQ=255)
                                                                                                                      tataAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTc                                 >  1:1374599/1‑150 (MQ=255)
                                                                                                                      tataAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTc                                 >  1:1991143/1‑150 (MQ=255)
                                                                                                                       ataAGAAAGCAACTTAATTCCCGCAGAAAGATTTGTGCGGGTAAGTATTAGCTTAATTTTTCTAGAATTAATCAAGCGAGTAATCCAAACGATACCTACGGATTAGGGAATATCACATTCTCTAGATCATGAGATACGGTATAACGTTcc                                >  1:313870/1‑150 (MQ=255)
                                                                                                                       ataAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTcc                                <  1:1269972/150‑1 (MQ=255)
                                                                                                                        taAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTCCCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCct                               >  1:1230484/1‑150 (MQ=255)
                                                                                                                        taAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCct                               >  2:1584102/1‑150 (MQ=255)
                                                                                                                         aaGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCctc                              >  2:756980/1‑150 (MQ=255)
                                                                                                                            aaaGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATATTTTCGAGCATTAATACCGCGCGTAATCCCAACGCTTCCAACGGATTAGGGAATAACACAAACTCTACATCATGGTTTACGGTAAAACGTTCCTCTcc                           >  2:1950742/1‑150 (MQ=255)
                                                                                                                              aGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATCACCCATACTCTACTTCAGGGGTTACGGTAAAACGTTCCTCTCCgg                         >  2:137886/1‑149 (MQ=255)
                                                                                                                              aGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGt                         <  1:1978178/150‑1 (MQ=255)
                                                                                                                                  aCTTAATACCCGCCCAATGATTTCTGCGTGTAAGTATTAGCTTATTTTTTCGAGCATAAATACAGCGCTTAATCCCAACGCTACCAACGGATTAGGGAATAACAAATACTCTACATAATGGGTTACGGTAAAACGTTCCTCTCCGTCCTg                     <  2:83092/150‑1 (MQ=255)
                                                                                                                                  aCTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTg                     >  1:1934614/1‑150 (MQ=255)
                                                                                                                                  aCTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTg                     >  2:1680712/1‑150 (MQ=255)
                                                                                                                                      aaTACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGcc                 >  2:671758/1‑150 (MQ=255)
                                                                                                                                       aTACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCa                <  1:1565924/150‑1 (MQ=255)
                                                                                                                                          cccGCAGAAATCATTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCa             >  1:1306917/1‑150 (MQ=255)
                                                                                                                                          cccGCAGAAATCATTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCa             >  1:582409/1‑150 (MQ=255)
                                                                                                                                             gCAGTATGTTTTCTTCGTGTAAGTATTAGCTTATTTTTTCGATCATTAATCCCGCGCGTTATCGCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATg          <  2:925908/150‑1 (MQ=255)
                                                                                                                                               aGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTAGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGTAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGtt        <  2:1355519/150‑1 (MQ=255)
                                                                                                                                                gAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGCATTAGGGAATACCACATACTCGACTTCAGGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGATc       >  2:956897/1‑150 (MQ=255)
                                                                                                                                                   tGATTTGTGGGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGTTCCtt    <  2:185818/150‑1 (MQ=255)
                                                                                                                                                   tGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGTTCCtt    <  1:1601859/150‑1 (MQ=255)
                                                                                                                                                    gATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGTTCCttt   <  1:336938/150‑1 (MQ=255)
                                                                                                                                                     aTTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGTTCCTTtc  <  1:94451/150‑1 (MQ=255)
                                                                                                                                                     aTTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGTTCCTTtc  >  1:558490/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGTTCCTTTC  >  NC_000913/1825789‑1826087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: