Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,825,939 T→A 5.7% intergenic (‑314/+16) spy ← / ← astE periplasmic ATP‑independent protein refolding chaperone, stress‑induced/succinylglutamate desuccinylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,825,9390TA 5.7% 308.1 / 7.1 106intergenic (‑314/+16)spy/astEperiplasmic ATP‑independent protein refolding chaperone, stress‑induced/succinylglutamate desuccinylase
Reads supporting (aligned to +/- strand):  ref base T (53/47);  new base A (2/4);  total (55/51)
Fisher's exact test for biased strand distribution p-value = 4.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.61e-01

Only 100 of 107 total aligned reads displayed.

ATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGTTCCTTTCT  >  NC_000913/1825793‑1826088
                                                                                                                                                  |                                                                                                                                                     
aTGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTc                                                                                                                                                    <  2:991480/150‑1 (MQ=255)
    agagTGTTGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGACCTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCg                                                                                                                                                <  2:1093612/150‑1 (MQ=255)
    agagTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCg                                                                                                                                                <  1:49212/150‑1 (MQ=255)
     gagTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAAATCATTCTGCgg                                                                                                                                               <  1:563722/150‑1 (MQ=255)
     gagTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAAATCATTCTGCgg                                                                                                                                               <  1:556008/150‑1 (MQ=255)
     gagTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAAATCATTCTGCgg                                                                                                                                               <  1:1501600/150‑1 (MQ=255)
         gatgaGATAATATTTCTTCACGATGATTTTGCAGAGATTATGAAGAACAATACCGGATGACTGGTGATAACTAAAGCAAATAACCAGGATTAATCTGTAGTAATTTATAAGAAAGCAACTAAATACACGCAGAAATCATTCTGCGGGTat                                                                                                                                           <  2:320602/150‑2 (MQ=255)
           tgaGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGt                                                                                                                                         <  2:438099/150‑1 (MQ=255)
             agagaATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTAt                                                                                                                                       >  1:1875874/1‑150 (MQ=255)
                 aaTATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGc                                                                                                                                   <  1:842383/150‑1 (MQ=255)
                  atatTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCt                                                                                                                                  <  1:1052728/150‑1 (MQ=255)
                     ttACAACACGATGATTTTGCAGAGATTATGTAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTAt                                                                                                                               >  1:1247062/1‑150 (MQ=255)
                       acaacaCGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTAttt                                                                                                                             <  1:510237/150‑1 (MQ=255)
                       acaacaCGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTAttt                                                                                                                             >  2:1554634/1‑150 (MQ=255)
                        caacaCGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAATCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTAtttt                                                                                                                            >  1:1083237/1‑150 (MQ=255)
                           cacGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTc                                                                                                                         <  1:1685338/150‑1 (MQ=255)
                            acGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCg                                                                                                                        <  2:256935/150‑1 (MQ=255)
                            acGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCg                                                                                                                        <  1:758383/150‑1 (MQ=255)
                             cGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGa                                                                                                                       >  1:1793091/1‑150 (MQ=255)
                              gatgatTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAg                                                                                                                      <  2:760739/150‑1 (MQ=255)
                               atgatTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGc                                                                                                                     <  2:1388658/150‑1 (MQ=255)
                                 gatTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCAt                                                                                                                   <  1:1376682/150‑1 (MQ=255)
                                   ttttGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTa                                                                                                                 >  1:1224791/1‑150 (MQ=255)
                                      tGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGATTATTTTTTCTAGCATTAAta                                                                                                              >  1:1760345/1‑149 (MQ=255)
                                          gagaTTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCcgc                                                                                                          >  1:2614/1‑150 (MQ=255)
                                          gagaTTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGAAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCcgc                                                                                                          >  2:723333/1‑150 (MQ=255)
                                           ataTTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCcgcg                                                                                                         <  2:158978/148‑1 (MQ=255)
                                           agaTTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCcgcg                                                                                                         >  1:485842/1‑150 (MQ=255)
                                                  gaagaaCTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGTGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATc                                                                                                  >  2:1646208/1‑150 (MQ=255)
                                                  gaagaaCTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACACGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTCTTTCGAGCATTAATCCCGCGCGTAATc                                                                                                  >  2:1658535/1‑150 (MQ=255)
                                                   aagaaCTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATcc                                                                                                 <  2:1773249/150‑1 (MQ=255)
                                                      aaCTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCaa                                                                                              <  2:1289921/150‑1 (MQ=255)
                                                       aCTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAAc                                                                                             <  2:453568/150‑1 (MQ=255)
                                                        atataCCGTATGACTGGTGATAAATAAATCAAATAACCAGTATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACg                                                                                            <  2:588544/149‑1 (MQ=255)
                                                         tataCCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCTAGCATTAATCCCGCGCGTAATCCCAACGc                                                                                           >  1:1202445/1‑150 (MQ=255)
                                                         tataCCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGc                                                                                           >  1:1956646/1‑150 (MQ=255)
                                                         tataCCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGc                                                                                           <  2:788219/150‑1 (MQ=255)
                                                          ataCCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCt                                                                                          >  2:1119359/1‑150 (MQ=255)
                                                          ataCCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCt                                                                                          >  2:336938/1‑150 (MQ=255)
                                                          ataCCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCt                                                                                          <  2:516464/150‑1 (MQ=255)
                                                          ataCCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCt                                                                                          >  2:753893/1‑150 (MQ=255)
                                                           taactgATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGAGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTa                                                                                         <  2:958013/145‑1 (MQ=255)
                                                             ccGGATGACTTGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCTGTTAATTATTAGCTAATTTTTTAGAGCATTAATCACTCGCGTAATCCCAACTCTAcc                                                                                       >  1:987756/1‑150 (MQ=255)
                                                                 aTGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACg                                                                                   <  2:283079/150‑1 (MQ=255)
                                                                  tGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACgg                                                                                  <  2:1868819/150‑1 (MQ=255)
                                                                       ggTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAg                                                                             >  1:1697948/1‑150 (MQ=255)
                                                                       ggTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAg                                                                             <  1:1767912/150‑1 (MQ=255)
                                                                       ggTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGAGTGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAAAGGATTAg                                                                             >  1:40767/1‑150 (MQ=255)
                                                                        gTGATAAATAAAGCATATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGAGTAATCCCAACGCTACCAACGGATTAgg                                                                            <  2:1818123/150‑1 (MQ=255)
                                                                         tGATAAATAAAGCAACTAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAggg                                                                           <  2:925357/150‑1 (MQ=255)
                                                                          gATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGa                                                                          <  2:204277/150‑1 (MQ=255)
                                                                            taaataaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAAt                                                                        <  1:1479204/150‑1 (MQ=255)
                                                                            taaataaaGCAAATAACCAGGATTAATCTGAATTAATTTATAAGAAAGCAACTTAATAACCGCAGAATGATTACTGCGGGTAAGTATTAGATTATTTTTTACAGAATTAATCACTCGCGTAATCACAAAGCTACAAACGGATTAGGGAAt                                                                        >  1:1434012/1‑150 (MQ=255)
                                                                              aataaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCACCGGATTAGGGAATaa                                                                      >  2:1419396/1‑150 (MQ=255)
                                                                               ataaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAac                                                                     <  1:1874843/150‑1 (MQ=255)
                                                                                 aaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACAAACGGATTAGGGAATAacaa                                                                   >  2:329487/1‑149 (MQ=255)
                                                                                   aGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACAt                                                                 >  2:1957777/1‑150 (MQ=255)
                                                                                    gCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGATTATATTTTCGAGCATTAATCCCGCTCGTAATCCAAACTCTACAAACGTATTAGGGAATAACACATa                                                                >  1:109413/1‑150 (MQ=255)
                                                                                     cAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGAGTGTAATTATTAGCTTATTTTATAGAGCATTAATCCCGCTCGTAATCCCAACGCTACAAACGGATTAGGGAATAACACATAc                                                               >  1:185818/1‑150 (MQ=255)
                                                                                      aaaTAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATAct                                                              <  1:25871/150‑1 (MQ=255)
                                                                                          aaCCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTAc                                                          >  1:275248/1‑150 (MQ=255)
                                                                                          aaCCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATATCTGCGGGTAAGTATTAGCTTATTTTATCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTAc                                                          <  2:1154806/150‑1 (MQ=255)
                                                                                           aCCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATACCGCGCGTAATCCCAACGCTACCAACTGATAAGGGAAAAACACATACTCTAcc                                                         >  2:197685/1‑149 (MQ=255)
                                                                                             cAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTAcatc                                                       <  2:817703/150‑1 (MQ=255)
                                                                                                  ttAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATggg                                                  <  1:168496/150‑1 (MQ=255)
                                                                                                    aaTCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGtt                                                >  2:1832620/1‑150 (MQ=255)
                                                                                                     aTCTGTATTAATTTATTAGAATGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGCTTa                                               >  1:306152/1‑150 (MQ=255)
                                                                                                      tCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTAc                                              <  1:989119/150‑1 (MQ=255)
                                                                                                         gTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGt                                           >  2:1369451/1‑150 (MQ=255)
                                                                                                          tATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATCGGTTACGGTa                                          >  2:1117541/1‑150 (MQ=255)
                                                                                                            ttAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCATCGGATTAGGGATTAACACATACTCTACATCATGGGTTACGGTaaa                                        >  2:1791615/1‑150 (MQ=255)
                                                                                                               aTTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGATTAACACATACTCTACATCATGGGTTACGGTAAAACg                                     >  2:1087183/1‑150 (MQ=255)
                                                                                                                 ttATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGtt                                   >  2:1060270/1‑150 (MQ=255)
                                                                                                                  tataAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTc                                  >  1:1991143/1‑150 (MQ=255)
                                                                                                                  tataAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTc                                  >  1:1374599/1‑150 (MQ=255)
                                                                                                                   ataAGAAAGCAACTTAATTCCCGCAGAAAGATTTGTGCGGGTAAGTATTAGCTTAATTTTTCTAGAATTAATCAAGCGAGTAATCCAAACGATACCTACGGATTAGGGAATATCACATTCTCTAGATCATGAGATACGGTATAACGTTcc                                 >  1:313870/1‑150 (MQ=255)
                                                                                                                   ataAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTcc                                 <  1:1269972/150‑1 (MQ=255)
                                                                                                                    taAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTCCCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCct                                >  1:1230484/1‑150 (MQ=255)
                                                                                                                    taAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCct                                >  2:1584102/1‑150 (MQ=255)
                                                                                                                     aaGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCctc                               >  2:756980/1‑150 (MQ=255)
                                                                                                                        aaaGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATATTTTCGAGCATTAATACCGCGCGTAATCCCAACGCTTCCAACGGATTAGGGAATAACACAAACTCTACATCATGGTTTACGGTAAAACGTTCCTCTcc                            >  2:1950742/1‑150 (MQ=255)
                                                                                                                          aGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATCACCCATACTCTACTTCAGGGGTTACGGTAAAACGTTCCTCTCCgg                          >  2:137886/1‑149 (MQ=255)
                                                                                                                          aGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGt                          <  1:1978178/150‑1 (MQ=255)
                                                                                                                              aCTTAATACCCGCCCAATGATTTCTGCGTGTAAGTATTAGCTTATTTTTTCGAGCATAAATACAGCGCTTAATCCCAACGCTACCAACGGATTAGGGAATAACAAATACTCTACATAATGGGTTACGGTAAAACGTTCCTCTCCGTCCTg                      <  2:83092/150‑1 (MQ=255)
                                                                                                                              aCTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTg                      >  1:1934614/1‑150 (MQ=255)
                                                                                                                              aCTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTg                      >  2:1680712/1‑150 (MQ=255)
                                                                                                                                  aaTACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGcc                  >  2:671758/1‑150 (MQ=255)
                                                                                                                                   aTACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCa                 <  1:1565924/150‑1 (MQ=255)
                                                                                                                                      cccGCAGAAATCATTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCa              >  1:582409/1‑150 (MQ=255)
                                                                                                                                      cccGCAGAAATCATTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCa              >  1:1306917/1‑150 (MQ=255)
                                                                                                                                         gCAGTATGTTTTCTTCGTGTAAGTATTAGCTTATTTTTTCGATCATTAATCCCGCGCGTTATCGCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATg           <  2:925908/150‑1 (MQ=255)
                                                                                                                                           aGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTAGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGTAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGtt         <  2:1355519/150‑1 (MQ=255)
                                                                                                                                            gAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGCATTAGGGAATACCACATACTCGACTTCAGGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGATc        >  2:956897/1‑150 (MQ=255)
                                                                                                                                             aaTGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGTTcc       <  2:663367/150‑1 (MQ=255)
                                                                                                                                               tGATTTGTGGGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGTTCCtt     <  2:185818/150‑1 (MQ=255)
                                                                                                                                               tGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGTTCCtt     <  1:1601859/150‑1 (MQ=255)
                                                                                                                                                gATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGTTCCttt    <  1:336938/150‑1 (MQ=255)
                                                                                                                                                 aTTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGTTCCTTtc   <  1:94451/150‑1 (MQ=255)
                                                                                                                                                 aTTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGTTCCTTtc   >  1:558490/1‑150 (MQ=255)
                                                                                                                                                  tttCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGGTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGTTCCTTtct  >  2:494409/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
ATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGTTCCTTTCT  >  NC_000913/1825793‑1826088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: