Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,829,328 C→G 6.2% G136A (GGC→GCC)  astD ← succinylglutamic semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,829,3280CG 6.2% 481.6 / 12.7 150G136A (GGC→GCC) astDsuccinylglutamic semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (70/70);  new base G (3/7);  total (73/77)
Fisher's exact test for biased strand distribution p-value = 3.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 151 total aligned reads displayed.

GCCATAAACGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCG  >  NC_000913/1829182‑1829475
                                                                                                                                                  |                                                                                                                                                   
gcCATAAACGCATTACCGCTTCGCCACTCCACGGGGTCAGTTCGCTGGGTTTAAAGATAATGGATTTACCTGACAGCAATGCCGGCACGCTATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCACACCCCGCCCGCACGccct                                                                                                                                                  >  1:1249106/1‑148 (MQ=255)
    tAAACGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCgg                                                                                                                                              >  2:1316434/1‑150 (MQ=255)
    tAAACGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCgg                                                                                                                                              >  1:189583/1‑150 (MQ=255)
     aaaCGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCggg                                                                                                                                             <  2:1291322/150‑1 (MQ=255)
        cGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCATTGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCCGCACGGCGTGCTGGCgg                                                                                                                                          <  2:63185/150‑2 (MQ=255)
          ccttACCGCTTCGCCACTCCACGCTGTCAGTTCGCTTGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGACCGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCCGCACGGCGTGCTGGCGGTg                                                                                                                                        <  1:281273/148‑1 (MQ=255)
           aTTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACTCCGTGCGGGCGATGt                                                                                                                                       >  1:261487/1‑150 (MQ=255)
            ttACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTc                                                                                                                                      <  2:1889635/150‑1 (MQ=255)
             tACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCg                                                                                                                                     <  1:861555/150‑1 (MQ=255)
              aCCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGCACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGc                                                                                                                                    >  2:119736/1‑150 (MQ=255)
               ccGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCTGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGACCGGCCGCTGTCGCa                                                                                                                                   >  2:1671393/1‑150 (MQ=255)
                cGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACATCGCCCGCACGGCGTGCTGGCGgtgtttggg                                                                                                                                  <  1:1153274/150‑6 (MQ=255)
                cGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAg                                                                                                                                  >  2:147068/1‑150 (MQ=255)
                cGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATCGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACATCGCCCGCACGGCGTGCTGGCGgtgtttggg                                                                                                                                  <  1:966466/150‑6 (MQ=255)
                cGCTACGCCACTCCCCGGTGTCAGTTCGCTGGGTTTAAAGCTAATGGTGTTACCTGCCAGCAATGCCGGAACGATCTGTCCGTGCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAg                                                                                                                                  <  2:338852/150‑1 (MQ=255)
                 gCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAgg                                                                                                                                 <  1:1315810/150‑1 (MQ=255)
                  cTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACATCGCCCGCACGGCGTGCTGGCGgtgtttgggcc                                                                                                                                <  2:1735221/150‑8 (MQ=39)
                     cGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTcg                                                                                                                             >  2:1061258/1‑150 (MQ=255)
                      gCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTcgc                                                                                                                            <  2:1979803/150‑1 (MQ=255)
                      gCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTcgc                                                                                                                            >  2:1041843/1‑150 (MQ=255)
                      gCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTcgc                                                                                                                            <  2:841451/150‑1 (MQ=255)
                         aCTCCACGGTGTCAGTTCGCTGGGGTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTcgccgc                                                                                                                         <  2:1505188/150‑1 (MQ=255)
                          cTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTcgccgcg                                                                                                                        <  1:1741773/150‑1 (MQ=255)
                          cTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTcgccgcg                                                                                                                        >  2:1263772/1‑150 (MQ=255)
                            ccACGGTGTCAGTTCGCTGGGTTTAAAGATAACGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTcgccgcgcc                                                                                                                      >  2:1567212/1‑150 (MQ=255)
                                  tgtCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCgg                                                                                                                <  1:488936/150‑1 (MQ=255)
                                   gtCAGATCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGc                                                                                                               <  1:692335/150‑1 (MQ=255)
                                    tCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCa                                                                                                              >  2:72334/1‑150 (MQ=255)
                                     cAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCAt                                                                                                             >  2:1131696/1‑150 (MQ=255)
                                       gTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCAttt                                                                                                           >  1:5163/1‑150 (MQ=255)
                                        ttCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTc                                                                                                          >  2:1070856/1‑150 (MQ=255)
                                        ttCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTc                                                                                                          <  2:67252/150‑1 (MQ=255)
                                          cGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCAc                                                                                                        <  2:515310/150‑1 (MQ=255)
                                          cGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCAc                                                                                                        <  2:1010572/150‑1 (MQ=255)
                                          cGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCAc                                                                                                        >  1:1125731/1‑150 (MQ=255)
                                             tgtgTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGGTCGGCAAATGACCAGGGAAATTATACGCCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTAc                                                                                                     <  1:1255252/147‑1 (MQ=255)
                                               ggTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACgc                                                                                                   <  1:1243410/150‑1 (MQ=255)
                                                gTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACgct                                                                                                  >  2:1578653/1‑150 (MQ=255)
                                                gTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACgct                                                                                                  >  2:653184/1‑150 (MQ=255)
                                                   tAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACgctgct                                                                                               >  2:310697/1‑150 (MQ=255)
                                                      aGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATAAGGCCCAAACACCGCCAGCACGCCGTTCTGGCTATGTCGCAGTCTCGCCGCGCCGTCCGGCATTTCACTACGATTCTCGc                                                                                            >  2:1758407/1‑150 (MQ=255)
                                                       caTAATGGTGCTACCTGCCAGCAATGCCGGCACGAGATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCTAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGcc                                                                                           <  2:324744/149‑1 (MQ=255)
                                                         tAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCgg                                                                                         >  2:467673/1‑150 (MQ=255)
                                                         tAATGGTGCTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCATCCCGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTCCGCTGCTCGCCgg                                                                                         <  1:87190/150‑1 (MQ=255)
                                                          aaTGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGt                                                                                        <  1:613988/150‑1 (MQ=255)
                                                           aTGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTa                                                                                       <  1:215728/150‑1 (MQ=255)
                                                                  tACCTGCCAGCAATGCCGGACCGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGt                                                                                >  2:1460674/1‑150 (MQ=255)
                                                                  tACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGt                                                                                >  2:1466496/1‑150 (MQ=255)
                                                                   aCCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTg                                                                               <  2:634786/150‑1 (MQ=255)
                                                                    ccTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGa                                                                              >  2:425370/1‑150 (MQ=255)
                                                                     cTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGAt                                                                             <  1:1191052/150‑1 (MQ=255)
                                                                      tGCCAGCAATGCTGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGCGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGACGGTACGAACGTGATa                                                                            >  2:1803768/1‑150 (MQ=255)
                                                                        ccAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACg                                                                          <  2:1375443/150‑1 (MQ=255)
                                                                        ccAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACg                                                                          >  2:769756/1‑150 (MQ=255)
                                                                           gCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCt                                                                       >  2:1674140/1‑150 (MQ=255)
                                                                            cAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCtt                                                                      <  1:714192/150‑1 (MQ=255)
                                                                                 gcGGAACGATATTTCCGTTCGGCATATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGAGGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTg                                                                 <  2:1849973/149‑1 (MQ=255)
                                                                                 ccGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGGCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTg                                                                 <  2:844187/150‑1 (MQ=255)
                                                                                 ccGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTg                                                                 >  2:189602/1‑150 (MQ=255)
                                                                                 ccGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTg                                                                 >  1:1457948/1‑150 (MQ=255)
                                                                                  cGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGa                                                                >  2:1434154/1‑150 (MQ=255)
                                                                                  cGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGa                                                                >  2:362657/1‑150 (MQ=255)
                                                                                  cGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGACGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGa                                                                >  2:1377672/1‑150 (MQ=255)
                                                                                   ggAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGat                                                               >  1:496719/1‑150 (MQ=255)
                                                                                          tatGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGa                                                        >  1:511408/1‑150 (MQ=255)
                                                                                            tGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGAtt                                                      >  2:1145142/1‑150 (MQ=255)
                                                                                                 gTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATTTCGCGATTTTAtt                                                 <  2:150026/150‑1 (MQ=255)
                                                                                                 gTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTAtt                                                 <  1:88826/150‑1 (MQ=255)
                                                                                                  ttCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTg                                                >  2:960706/1‑150 (MQ=255)
                                                                                                   tCGGCAAATGACCAGGGAAATTATACTGCCCAAACACCGCCCGCACGCCGTGCGGGCGATGTCGCCGGCTCGCCGCGCCGTCCGGCAGTTCCATACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGAGATCGCGAGTTTATTgt                                               >  1:1010443/1‑149 (MQ=255)
                                                                                                          aTGACCAGGGAAATTTTACGGCCCAAACACGGCCAGCACGCCGTGAGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCTCTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGc                                        <  2:172623/150‑1 (MQ=255)
                                                                                                           tGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGcc                                       <  1:1379391/150‑1 (MQ=255)
                                                                                                            gACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCg                                      <  1:1809441/150‑1 (MQ=255)
                                                                                                            gACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCg                                      <  1:911524/150‑1 (MQ=255)
                                                                                                            gACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCg                                      <  1:1208890/150‑1 (MQ=255)
                                                                                                                aGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCAc                                  >  2:53215/1‑150 (MQ=255)
                                                                                                                  ggAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACtt                                >  1:1696664/1‑150 (MQ=255)
                                                                                                                  ggAAATTATACGGCCCAAACACCGCCAGCACACCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGAATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGAGATTATATTGATCATCTCCGTCACtt                                >  2:2012990/1‑150 (MQ=255)
                                                                                                                    aaaTTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCCCTACGCTGCTCGCCGGTACGCACGGGATACGCCGTAATTGATATCGCGATATTATTGCTCATCGCCGTCACTTCg                              >  1:1416830/1‑150 (MQ=255)
                                                                                                                     aaTTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGCTACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCgg                             >  1:1972637/1‑150 (MQ=255)
                                                                                                                     aaTTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCgg                             <  1:723294/150‑1 (MQ=255)
                                                                                                                     aaTTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCgg                             <  1:824761/150‑1 (MQ=255)
                                                                                                                      aTTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGt                            <  1:2002476/150‑1 (MQ=255)
                                                                                                                          taCGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGcc                        >  2:1716257/1‑150 (MQ=255)
                                                                                                                           aCGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCg                       <  1:1674140/150‑1 (MQ=255)
                                                                                                                              gCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCTGCATTTCACTACGCTGCTCGCCGGTACGCACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCtt                    >  2:145714/1‑150 (MQ=255)
                                                                                                                                ccAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCGCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTcc                  <  2:1388624/150‑1 (MQ=255)
                                                                                                                                      atcGCCCGCACGGCGTGCTGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAgcgc            >  1:1864296/3‑150 (MQ=255)
                                                                                                                                      atcGCCCGCACGGCGTGCTGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAgcgc            >  1:1640897/3‑150 (MQ=255)
                                                                                                                                      atcGCCCGCACGGCGTGCTGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAgcgc            >  1:1321260/3‑150 (MQ=255)
                                                                                                                                        cGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCTGTTGCCGCTTCCCAGCGCgg          <  1:1803768/150‑1 (MQ=255)
                                                                                                                                        cGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCgg          >  2:486568/1‑150 (MQ=255)
                                                                                                                                         gCCAGCACGCCGTGCGGGCGATGTCTCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGc         <  2:261487/150‑1 (MQ=255)
                                                                                                                                         gCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTAAGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCTCTTCGGTTGCCTCTTCCCCGCGCGGc         >  1:1016987/1‑150 (MQ=255)
                                                                                                                                          ccAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCt        <  1:1978966/150‑1 (MQ=255)
                                                                                                                                          ccAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCt        >  1:128461/1‑150 (MQ=255)
                                                                                                                                          ccAGCACGCCGTGCGGGCGATGTCGCAGGCGCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGACTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCt        <  2:1253622/150‑1 (MQ=255)
                                                                                                                                             gCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTAc     >  1:49447/1‑150 (MQ=255)
                                                                                                                                               aCGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTAccc   <  1:1070856/150‑1 (MQ=255)
                                                                                                                                                cGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCg  >  1:379103/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GCCATAAACGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCG  >  NC_000913/1829182‑1829475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: