Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,851,204 A→C 5.6% T115T (ACA→ACC)  ansA → cytoplasmic L‑asparaginase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,851,2040AC 5.6% 421.0 / 9.7 134T115T (ACA→ACC) ansAcytoplasmic L‑asparaginase 1
Reads supporting (aligned to +/- strand):  ref base A (78/48);  new base C (4/4);  total (82/52)
Fisher's exact test for biased strand distribution p-value = 7.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

GAAGACTGGCAGCATATTGCTGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCC  >  NC_000913/1851055‑1851351
                                                                                                                                                     |                                                                                                                                                   
gAAGACTGGCAGCATATTGCTGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACa                                                                                                                                                     >  2:1451652/1‑150 (MQ=255)
 aaGACTGGCAGCATATTGCTGAAGATATTAAAGCGCACTATGACGACTATGAGGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAg                                                                                                                                                    <  1:100349/150‑1 (MQ=255)
    aCTGGCAGCATATTGCTGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGt                                                                                                                                                 >  2:1846813/1‑150 (MQ=255)
     cTGGCAGCATATTGCTGAAGATATTAAAGCGCACTATGACGACTATTATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTc                                                                                                                                                <  2:439533/150‑1 (MQ=255)
     cTGGCAGCATATTGCTGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACTGCACCAACACGATGGCGTATACCGCCTCGGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGAAACGGTc                                                                                                                                                >  2:324408/1‑150 (MQ=255)
     cTGGCAGCATATTGCTGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTc                                                                                                                                                >  1:39216/1‑150 (MQ=255)
          agcaTATTGCTGAAGATAGTTAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACCCTGTCACaat                                                                                                                                           <  2:563091/150‑2 (MQ=255)
           gcaTATTGCTGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAAt                                                                                                                                          >  1:838268/1‑150 (MQ=255)
            caTATTGCTGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACCCTGTCACaatga                                                                                                                                         <  1:1929431/150‑4 (MQ=255)
              tatTGCTGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACCCTGTCACaatgacc                                                                                                                                       <  1:555432/150‑6 (MQ=255)
              tatTGCTGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGGCATTGTGACAGGGTCACAAATccc                                                                                                                                       <  2:1027937/150‑1 (MQ=255)
              tatTGCTGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGATAGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATcac                                                                                                                                       >  1:1847529/1‑148 (MQ=255)
              aatTGCTGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATATCGGTAAACAGGTCATTCTGACAGGGTCACAAATccc                                                                                                                                       >  1:1480152/2‑150 (MQ=255)
               atTGCTGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACCCTGTCACaatgaccg                                                                                                                                      <  2:1660340/150‑7 (MQ=255)
                 tGCTGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCgct                                                                                                                                    >  1:1381406/1‑150 (MQ=255)
                    tGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCgctggc                                                                                                                                 >  2:826516/1‑150 (MQ=255)
                    tGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCgctggc                                                                                                                                 >  1:1989579/1‑150 (MQ=255)
                    tGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACAGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCACgctggc                                                                                                                                 >  1:691168/1‑150 (MQ=255)
                     gAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGATCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCgctggct                                                                                                                                >  2:1338920/1‑150 (MQ=255)
                          tatTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGtt                                                                                                                           >  1:1004451/1‑150 (MQ=255)
                            ttAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGATGAGTTAc                                                                                                                         >  2:1472406/1‑150 (MQ=255)
                            ttAAAGCGAACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGAAGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCTGTAAACCGGTCATAGTGACAGGGTCACAAATCCCGCTGGCTTAGTTaa                                                                                                                         >  1:750657/1‑149 (MQ=255)
                              aaaGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGTCGTATACCGCCTCTGCGCTGTCGTTCATTCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGc                                                                                                                       <  2:1631294/150‑1 (MQ=255)
                              aaaGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGATAATCTCGGTAAACCTGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTActc                                                                                                                       >  2:47962/1‑148 (MQ=255)
                                  cgcCCTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTg                                                                                                                   <  2:235308/150‑1 (MQ=255)
                                   gcACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGa                                                                                                                  <  2:1568150/150‑1 (MQ=255)
                                    cACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGTTGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTGTGAc                                                                                                                 <  1:1544501/150‑1 (MQ=255)
                                         tGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACa                                                                                                            >  1:1675036/1‑150 (MQ=255)
                                            cgacTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAAt                                                                                                         >  1:22139/1‑150 (MQ=255)
                                            cgacTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACAGCCTCTGCGCTGTCGTTGATGCTAGAGAATCTCGGTAACCCCGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGCCGGACAAAt                                                                                                         >  1:87452/1‑150 (MQ=255)
                                             gacTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGGCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAAtt                                                                                                        <  2:83111/150‑1 (MQ=255)
                                                  tgatgGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATct                                                                                                   >  1:488498/1‑150 (MQ=255)
                                                    atgGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATctgc                                                                                                 <  2:1029933/150‑1 (MQ=255)
                                                          ttGTCATTCTGCACGTCACCGACACGATGGGGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGTATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCACTATGGCGGAGATACGCTCTGACGGACAAATTAATCTGCTGAATg                                                                                           <  2:107239/150‑1 (MQ=255)
                                                           tGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGc                                                                                          >  1:1111253/1‑150 (MQ=255)
                                                           tGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGc                                                                                          >  1:776599/1‑150 (MQ=255)
                                                                  cTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTAc                                                                                   >  2:987235/1‑150 (MQ=255)
                                                                   tGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACg                                                                                  >  2:760761/1‑150 (MQ=255)
                                                                   tGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACg                                                                                  >  1:574221/1‑150 (MQ=255)
                                                                   tGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCAGGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGCCGGACAAATTAATCTGCTGAATGCGTGGTACg                                                                                  >  1:136299/1‑150 (MQ=255)
                                                                    gCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCCCAAATCCCGCTGGCTGAGTTACGCTCTGACGGCCAAATTAATCTGCTGAATGCGTTGTACGt                                                                                 >  1:1223179/1‑150 (MQ=255)
                                                                     cACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGtt                                                                                >  2:1305142/1‑150 (MQ=255)
                                                                         gCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTgcgg                                                                            <  2:1291132/150‑1 (MQ=255)
                                                                          cACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTgcggc                                                                           >  2:257034/1‑150 (MQ=255)
                                                                          cACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTgcggc                                                                           >  1:1341563/1‑150 (MQ=255)
                                                                           aCCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGGCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTgcggcg                                                                          <  1:115103/150‑1 (MQ=255)
                                                                             cGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGAGTTGTACGTTGCGGCGaa                                                                        >  1:552853/1‑150 (MQ=255)
                                                                             cGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGATCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGaa                                                                        >  2:1348135/1‑150 (MQ=255)
                                                                              gACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAAt                                                                       <  1:1451652/150‑1 (MQ=255)
                                                                              gACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAAt                                                                       <  2:816169/150‑1 (MQ=255)
                                                                                cacGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTa                                                                     >  2:637261/1‑150 (MQ=255)
                                                                                cacGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTCCGGCGAATTa                                                                     >  2:352861/1‑150 (MQ=255)
                                                                                   gATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCGGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATcc                                                                  <  1:747607/150‑1 (MQ=255)
                                                                                     tGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGa                                                                <  1:575323/150‑1 (MQ=255)
                                                                                       gCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATc                                                              <  2:1989579/150‑1 (MQ=255)
                                                                                       gCGTATACCGCCTCTGCGCTGTCGTTCATGCTAGAGAAACTCGGTAAACCTGTCATTGTGACAGGGTCACAAAACCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAAACTTCTGAATGCGTTGTACGTTGCGGCGATTTATCCGATc                                                              >  1:362202/1‑150 (MQ=255)
                                                                                       gCGTATACCGCCGCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATc                                                              <  1:868189/150‑1 (MQ=255)
                                                                                        cGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCa                                                             <  1:773431/150‑1 (MQ=255)
                                                                                          tataCCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAAc                                                           <  1:1662244/150‑1 (MQ=255)
                                                                                           ataCCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACg                                                          <  2:576693/150‑1 (MQ=255)
                                                                                            taCCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGGCACAAATCCCGCTGGCTGAGTTGCGGTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGAGAATTTTCCGATGAAGGa                                                         >  2:119642/1‑150 (MQ=255)
                                                                                              ccGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAg                                                       >  2:703784/1‑150 (MQ=255)
                                                                                              ccGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAg                                                       >  1:618139/1‑150 (MQ=255)
                                                                                              ccGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTAACGCTCTGCCGGACAAATTAATCTGCTGACTGCGTGGAACGTTGCGGCGAATTCTCCGCTCAACGAAg                                                       >  1:382578/1‑150 (MQ=255)
                                                                                                  ctctGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAAc                                                   >  1:1042262/1‑150 (MQ=255)
                                                                                                            tCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCAAAAATCCCGCTGGCTGAGTTACTCTCTGACGGAAAAATTAATCTGCTGAATTCGTTGTACGTTGCGGCGAATTTTCCGATCAACGAAGTAACGCTCTTTTTc                                         <  1:1197677/150‑1 (MQ=255)
                                                                                                             cGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCa                                        >  2:1360469/1‑150 (MQ=255)
                                                                                                              gTTCATGCTCGCGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCaa                                       <  2:1387847/150‑1 (MQ=255)
                                                                                                              gTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCaa                                       >  1:419118/1‑150 (MQ=255)
                                                                                                                 cATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATaa                                    <  1:1967829/150‑1 (MQ=255)
                                                                                                                  aTGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAAc                                   <  1:469584/150‑1 (MQ=255)
                                                                                                                  aTGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGGCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAAc                                   <  2:1478685/150‑1 (MQ=255)
                                                                                                                   tGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAAcc                                  >  1:643097/1‑150 (MQ=255)
                                                                                                                    gCTCGAGAATCTCGGTAAACCGGTCATTGTGCCAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCg                                 >  1:950637/1‑150 (MQ=255)
                                                                                                                    gCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCg                                 >  1:1685705/1‑150 (MQ=255)
                                                                                                                     cTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGa                                >  1:1849913/1‑150 (MQ=255)
                                                                                                                     cTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGa                                >  2:1352362/1‑150 (MQ=255)
                                                                                                                     cTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGa                                >  1:867770/1‑150 (MQ=255)
                                                                                                                      tCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGAt                               >  1:798926/1‑150 (MQ=255)
                                                                                                                      tCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGAt                               >  1:220385/1‑150 (MQ=255)
                                                                                                                       cGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGAtt                              >  2:1902684/1‑150 (MQ=255)
                                                                                                                       cGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATATGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGAtt                              >  1:591199/1‑150 (MQ=255)
                                                                                                                        gagaATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTg                             >  1:660838/1‑150 (MQ=255)
                                                                                                                               tcGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATcgcg                      <  2:1123572/150‑1 (MQ=255)
                                                                                                                                 ggTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGc                    <  1:944762/150‑1 (MQ=255)
                                                                                                                                 ggTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGc                    <  2:506161/150‑1 (MQ=255)
                                                                                                                                    aaaCCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCacc                 >  1:499016/1‑148 (MQ=255)
                                                                                                                                      aCCGGTCATTGTGTCAGGGTCACAAATCCCGCTGGCTGACTTACGCTCTGAGGGACAAATTAATCTGCTGTATGCGTTGTACGTTGCGGCGAATTATCCGATCAACTAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCg               <  2:1480152/150‑1 (MQ=255)
                                                                                                                                      aCCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCg               <  2:454642/150‑1 (MQ=255)
                                                                                                                                         gggatttGTGACCCTGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCAc            >  1:682324/6‑150 (MQ=255)
                                                                                                                                         gggatttGTGACCCTGTCACAAATCCCGCTGGCTGAGTTACGCTCAGACGGACAAAGTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGAACAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCAc            >  2:1747284/6‑150 (MQ=255)
                                                                                                                                          ggatttGTGACCCTGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATACCCGATTGTATCGCGGCAACCGCACt           >  2:290073/5‑150 (MQ=255)
                                                                                                                                            cATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTAc         >  1:487473/1‑150 (MQ=255)
                                                                                                                                             aTTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTAcc        >  1:1140271/1‑150 (MQ=255)
                                                                                                                                                gtgACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCaaa     >  2:1621855/1‑150 (MQ=255)
                                                                                                                                                gtgACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGAGAATTATACGATCAACGAAGTAACGCTCTTTTTCAATAAACGATTGTATCGCGGCAACCGCACTACCaaa     >  1:1111477/1‑150 (MQ=255)
                                                                                                                                                gtgACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGAGGCGAATTAACCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCaaa     >  2:376175/1‑150 (MQ=255)
                                                                                                                                                gtgACAGCGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGATTTATCCGATCAACGAAGTACCGCTCTTTTTCAAAAACCGAGTTTATCGCGGCAACCGCCCTACCaac     >  2:968741/1‑149 (MQ=255)
                                                                                                                                                 tgACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAg    >  1:720816/1‑150 (MQ=255)
                                                                                                                                                  caCAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGc   <  2:1008749/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GAAGACTGGCAGCATATTGCTGAAGATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCC  >  NC_000913/1851055‑1851351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑