Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,854,021 A→G 5.5% intergenic (‑42/+75) ydjE ← / ← ydjF putative MFS sugar transporter, membrane protein/putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,854,0210AG 5.5% 332.4 / 7.2 109intergenic (‑42/+75)ydjE/ydjFputative MFS sugar transporter, membrane protein/putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (51/52);  new base G (2/4);  total (53/56)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.40e-01

Only 100 of 110 total aligned reads displayed.

AAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGTTA  >  NC_000913/1853872‑1854166
                                                                                                                                                     |                                                                                                                                                 
aaaCCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGTCaaa                                                                                                                                                   >  1:297580/1‑150 (MQ=255)
  aCCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAAtt                                                                                                                                                 >  1:1490372/1‑150 (MQ=255)
      tGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGTGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACt                                                                                                                                             >  2:498395/1‑150 (MQ=255)
        ttAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTg                                                                                                                                           <  1:1617526/150‑1 (MQ=255)
         taataaCAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCAATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGAGGCAAATTCACTTGc                                                                                                                                          >  1:1906203/1‑150 (MQ=255)
         taataaCAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCAAAAGGCAAGCGGTCCAGTCTTGCGACAATTTGATCATAATGTTCCATTAGGTGCCTCGGATTTGATAAAAAGGAAGCAATTGCTGCAAATTCACTTGc                                                                                                                                          >  1:1353764/1‑150 (MQ=255)
            taaCAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGc                                                                                                                                       >  2:809541/1‑150 (MQ=255)
             aaCAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCa                                                                                                                                      >  2:1897855/1‑150 (MQ=255)
              aCAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCAc                                                                                                                                              <  1:917442/141‑1 (MQ=255)
              aCAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCAc                                                                                                                                              >  2:917442/1‑141 (MQ=255)
               cacaCTAAAGCTTATAATACCAAATATACGATAAAGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAGTGAATTTGCCGCaat                                                                                                                                    <  1:521156/147‑3 (MQ=255)
               cAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCACAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAGTGAATTTGCCGCaat                                                                                                                                    <  1:528687/150‑3 (MQ=255)
                aGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAGTGAATTTGCCGCaatt                                                                                                                                   <  2:647539/150‑4 (MQ=255)
                aGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAGTGAATTTGCCGCaatt                                                                                                                                   <  2:1917048/150‑4 (MQ=255)
                aGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCt                                                                                                                                   >  2:1589188/1‑150 (MQ=255)
                  aCTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCttt                                                                                                                                 >  1:128999/1‑150 (MQ=255)
                   cTACAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGAGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGAAGCAATTGCGGCAAATTAACTTGCCGCATCATTa                                                                                                                                >  1:1255442/1‑150 (MQ=255)
                   cTAAAGCTTATAATACCAAATATACGATAATGAAACCGGTCCAAAGGCAAGCGGTCCAGACATCAGCCAATTTGATCATATAGTTCCATTAGGTGCCTCGCATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTa                                                                                                                                >  1:1255441/1‑150 (MQ=255)
                   cTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTa                                                                                                                                <  2:1844552/150‑1 (MQ=255)
                   atAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTa                                                                                                                                <  2:1006377/149‑1 (MQ=255)
                     ataGCTTATAATACCATATATACGATTATGAATCCGGGGCAATGTCTAGCGGTCCAGTCTTGTGCGAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAATGGTAGCAATTGCGGCATATTCACTTGCCGCATCTTTatc                                                                                                                              <  2:1631565/148‑1 (MQ=255)
                     aaaGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTatc                                                                                                                              <  2:1101555/150‑1 (MQ=255)
                      aaGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGAAAGCGGTCCATTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTatca                                                                                                                             >  2:572089/1‑150 (MQ=255)
                           tataATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCAtt                                                                                                                        >  2:569391/1‑150 (MQ=255)
                            ataataCCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCAttt                                                                                                                       >  2:1842791/1‑150 (MQ=255)
                              aataCCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTa                                                                                                                     <  1:1181272/150‑1 (MQ=255)
                              aataCCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTa                                                                                                                     >  1:459175/1‑150 (MQ=255)
                                 aCCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTAttt                                                                                                                  <  2:1866691/150‑1 (MQ=255)
                                 aCCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTAttt                                                                                                                  <  1:1526978/150‑1 (MQ=255)
                                  ccAAATATACGATAATGAAACCGGTCCAAATGCAAGCGGTCCAGTCTTGCGCCAATTTGATCTTATTGTTCCATTATGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTc                                                                                                                 <  2:643600/150‑1 (MQ=255)
                                  ccAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTc                                                                                                                 >  1:1027582/1‑150 (MQ=255)
                                   cAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCa                                                                                                                >  1:926653/1‑150 (MQ=255)
                                   cAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCa                                                                                                                >  2:1883342/1‑150 (MQ=255)
                                    aaaTATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCaa                                                                                                               >  1:808449/1‑150 (MQ=255)
                                     aaTATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAg                                                                                                              >  2:2003074/1‑150 (MQ=255)
                                      atatatGATAATGAAACCTGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATAAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCCAgg                                                                                                             <  1:159042/150‑1 (MQ=255)
                                       tataCGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGgc                                                                                                            <  2:1586155/150‑1 (MQ=255)
                                       tataCGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGgc                                                                                                            >  1:1804683/1‑150 (MQ=255)
                                         taCGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCACCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGgcgc                                                                                                          >  1:1457629/1‑150 (MQ=255)
                                          aCGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGGTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCa                                                                                                         <  1:379233/150‑1 (MQ=255)
                                           cGATAATGCAACCGGGCCAAAGGCAAGCGGTCCAGTCTTTCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAg                                                                                                        <  2:1941380/150‑1 (MQ=255)
                                           cGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGGCTCGGATTTGCTTAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCCTCTTGATCATCATTTTATTTCAAGGCGCAg                                                                                                        >  2:1269403/1‑150 (MQ=255)
                                            gATTATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAgg                                                                                                       <  2:128999/150‑1 (MQ=255)
                                              tAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGaa                                                                                                     <  1:168486/150‑1 (MQ=255)
                                               aaTGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAAc                                                                                                    <  2:1377556/150‑1 (MQ=255)
                                                   aaaCAGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAATGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAaat                                                                                                <  1:254064/150‑1 (MQ=255)
                                                        gggCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAatat                                                                                           <  2:411985/150‑1 (MQ=255)
                                                         ggCCAAATGCAAGCGGTCCAGTCTTGCTCCAATTTGATCATATTGTTCCATTATGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATAtt                                                                                          <  2:368409/150‑1 (MQ=255)
                                                          gCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTg                                                                                         <  1:1316752/150‑1 (MQ=255)
                                                             aaaGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACa                                                                                      >  2:73997/1‑150 (MQ=255)
                                                                  cAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACgtg                                                                                 >  2:744062/1‑150 (MQ=255)
                                                                    aGCGGTCCAGTCTTGCGCCAATTTGATCTTATTGTTCCATTAGGTGCCTCGGATTTGCTAAATAGGTAGCAATTGCGTCAAATTCACTTTCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGtgtg                                                                               <  2:1118814/150‑1 (MQ=255)
                                                                    aGCGGTCCAGTCTTGCGCCAATTTGATCATATTGATCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGtgtg                                                                               <  1:1840272/150‑1 (MQ=255)
                                                                       ggTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCAGAATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAAAGTGTGCtt                                                                            >  2:359542/1‑150 (MQ=255)
                                                                         tCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTaa                                                                          >  1:1766829/1‑150 (MQ=255)
                                                                         tCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTaa                                                                          >  1:526472/1‑150 (MQ=255)
                                                                           cAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAAt                                                                        >  1:1981054/1‑150 (MQ=255)
                                                                              tCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTAc                                                                     <  1:1641059/150‑1 (MQ=255)
                                                                              tCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTAc                                                                     <  2:1031257/150‑1 (MQ=255)
                                                                               attGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTAcc                                                                    <  2:807115/149‑1 (MQ=255)
                                                                                 tGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACcac                                                                  <  2:546718/150‑1 (MQ=255)
                                                                                   cgcCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCacac                                                                >  1:1986858/1‑150 (MQ=255)
                                                                                   cgcCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCacac                                                                >  2:319277/1‑150 (MQ=255)
                                                                                      cAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAg                                                             <  1:498395/150‑1 (MQ=255)
                                                                                      cAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAg                                                             <  1:1121604/150‑1 (MQ=255)
                                                                                        aTTTGATCATATTGTTCCATTTGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCt                                                           >  1:1431643/1‑150 (MQ=255)
                                                                                         tttGATCATATTTTTCCATTAGGTGCCTAGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTg                                                          <  2:292170/150‑1 (MQ=255)
                                                                                              tCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGatat                                                     <  1:1061712/150‑1 (MQ=255)
                                                                                                     gTTCCATTAGGTGCGTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACAACACCAGCTGGATATTATTGTc                                              >  1:1394708/1‑150 (MQ=255)
                                                                                                     gTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTATATTTCAAGGCGCAGGAACAAATAATATTGACACCGTGTGCTTAAATTACCACACCAGCGGGATATTATTGtg                                              >  2:143402/1‑149 (MQ=255)
                                                                                                         cATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTg                                          <  2:1838750/150‑1 (MQ=255)
                                                                                                          aTTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCTGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGc                                         <  1:809541/150‑1 (MQ=255)
                                                                                                            tAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCaa                                       >  1:2007046/1‑150 (MQ=255)
                                                                                                             aGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCAAACCAGCTGGATATTATTGTCTTTGCaaa                                      >  2:1003881/1‑150 (MQ=255)
                                                                                                               gTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAAt                                    <  2:794545/150‑1 (MQ=255)
                                                                                                                ttccTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATg                                   <  1:1450484/148‑1 (MQ=255)
                                                                                                                tGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATg                                   <  1:1601154/150‑1 (MQ=255)
                                                                                                                      ggATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATcc                             <  1:1764668/150‑1 (MQ=255)
                                                                                                                      ggATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATcc                             <  1:1883342/150‑1 (MQ=255)
                                                                                                                        aTTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCAc                           >  1:945898/1‑150 (MQ=255)
                                                                                                                             cTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGc                      <  2:297580/150‑1 (MQ=255)
                                                                                                                             cTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGc                      >  2:848427/1‑150 (MQ=255)
                                                                                                                              tAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCa                     >  1:580811/1‑150 (MQ=255)
                                                                                                                                aaaaGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATTTTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCAcc                   <  1:1808488/150‑1 (MQ=255)
                                                                                                                                aaaaGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTAACATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCAcc                   >  1:397206/1‑150 (MQ=255)
                                                                                                                                   aGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCgg                <  1:1589188/150‑1 (MQ=255)
                                                                                                                                    ggTAGCAATTGCGGCAAATTCACTTGCCGGATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGt               >  1:1228178/1‑150 (MQ=255)
                                                                                                                                    ggTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGt               <  1:2003074/150‑1 (MQ=255)
                                                                                                                                      tAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGa             <  2:808449/150‑1 (MQ=255)
                                                                                                                                       aGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGAt            >  1:737870/1‑150 (MQ=255)
                                                                                                                                         cAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGAttt          <  2:1457629/150‑1 (MQ=255)
                                                                                                                                          aaTTGCGGCAAATTCACTTGCCGCATCTTTATCATAATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTa         >  2:1679939/1‑150 (MQ=255)
                                                                                                                                             tGCGGCAAGTGAATTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCa      >  1:987758/1‑150 (MQ=255)
                                                                                                                                             tGCGGCAAGTGAATTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCa      >  2:149365/1‑150 (MQ=255)
                                                                                                                                             tGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCa      <  1:992046/150‑1 (MQ=255)
                                                                                                                                              gCGGCAAATTCAATTGCCGCATATTTATAATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACAAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTTTCAg     <  1:1214060/150‑1 (MQ=255)
                                                                                                                                               cGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCTGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGt    >  2:1501994/1‑150 (MQ=255)
                                                                                                                                                ggCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGtt   >  1:1248829/1‑150 (MQ=255)
                                                                                                                                                ggCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGtt   >  1:1889410/1‑150 (MQ=255)
                                                                                                                                                 gCAAATTCACTTGCCGCATCTTTATCATAATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTTTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGtta  >  1:1899209/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
AAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGTTA  >  NC_000913/1853872‑1854166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: