Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,854,023 T→G 5.6% intergenic (‑44/+73) ydjE ← / ← ydjF putative MFS sugar transporter, membrane protein/putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,854,0230TG 5.6% 327.6 / 7.1 107intergenic (‑44/+73)ydjE/ydjFputative MFS sugar transporter, membrane protein/putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (49/52);  new base G (2/4);  total (51/56)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.29e-01

Only 100 of 111 total aligned reads displayed.

ACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGTTATTATGT  >  NC_000913/1853874‑1854172
                                                                                                                                                     |                                                                                                                                                     
aCCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAAtt                                                                                                                                                       >  1:1490372/1‑150 (MQ=255)
 cccTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAAtta                                                                                                                                                      >  1:823277/1‑149 (MQ=255)
   cTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCAc                                                                                                                                                    >  2:1181272/1‑150 (MQ=255)
   cTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCAc                                                                                                                                                    >  1:385291/1‑150 (MQ=255)
    tGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGTGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACt                                                                                                                                                   >  2:498395/1‑150 (MQ=255)
      ttAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTg                                                                                                                                                 <  1:1617526/150‑1 (MQ=255)
       taataaCAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCAATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGAGGCAAATTCACTTGc                                                                                                                                                >  1:1906203/1‑150 (MQ=255)
       taataaCAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCAAAAGGCAAGCGGTCCAGTCTTGCGACAATTTGATCATAATGTTCCATTAGGTGCCTCGGATTTGATAAAAAGGAAGCAATTGCTGCAAATTCACTTGc                                                                                                                                                >  1:1353764/1‑150 (MQ=255)
          taaCAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGc                                                                                                                                             >  2:809541/1‑150 (MQ=255)
           aaCAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCa                                                                                                                                            >  2:1897855/1‑150 (MQ=255)
            aCAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCAc                                                                                                                                                    <  1:917442/141‑1 (MQ=255)
            aCAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCAc                                                                                                                                                    >  2:917442/1‑141 (MQ=255)
             cacaCTAAAGCTTATAATACCAAATATACGATAAAGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAGTGAATTTGCCGCaat                                                                                                                                          <  1:521156/147‑3 (MQ=255)
             cAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCACAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAGTGAATTTGCCGCaat                                                                                                                                          <  1:528687/150‑3 (MQ=255)
              aGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAGTGAATTTGCCGCaatt                                                                                                                                         <  2:1917048/150‑4 (MQ=255)
              aGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAGTGAATTTGCCGCaatt                                                                                                                                         <  2:647539/150‑4 (MQ=255)
              aGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCt                                                                                                                                         >  2:1589188/1‑150 (MQ=255)
                aCTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCttt                                                                                                                                       >  1:128999/1‑150 (MQ=255)
                aCTAAAGCTATTAATAACAAATATCCGATAATGAAACCGGGCCAAAGTCAAGCGGTCCAGGCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCttt                                                                                                                                       <  2:290397/150‑1 (MQ=255)
                 cTACAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGAGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGAAGCAATTGCGGCAAATTAACTTGCCGCATCATTa                                                                                                                                      >  1:1255442/1‑150 (MQ=255)
                 cTAAAGCTTATAATACCAAATATACGATAATGAAACCGGTCCAAAGGCAAGCGGTCCAGACATCAGCCAATTTGATCATATAGTTCCATTAGGTGCCTCGCATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTa                                                                                                                                      >  1:1255441/1‑150 (MQ=255)
                 atAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTa                                                                                                                                      <  2:1006377/149‑1 (MQ=255)
                   ataGCTTATAATACCATATATACGATTATGAATCCGGGGCAATGTCTAGCGGTCCAGTCTTGTGCGAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAATGGTAGCAATTGCGGCATATTCACTTGCCGCATCTTTatc                                                                                                                                    <  2:1631565/148‑1 (MQ=255)
                   aaaGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTatc                                                                                                                                    <  2:1101555/150‑1 (MQ=255)
                    aaGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGAAAGCGGTCCATTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTatca                                                                                                                                   >  2:572089/1‑150 (MQ=255)
                         tataATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCAtt                                                                                                                              >  2:569391/1‑150 (MQ=255)
                          ataataCCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCAttt                                                                                                                             >  2:1842791/1‑150 (MQ=255)
                            aataCCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTa                                                                                                                           <  1:1181272/150‑1 (MQ=255)
                            aataCCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTa                                                                                                                           >  1:459175/1‑150 (MQ=255)
                               aCCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTAttt                                                                                                                        <  2:1866691/150‑1 (MQ=255)
                               aCCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTAttt                                                                                                                        <  1:1526978/150‑1 (MQ=255)
                                ccAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTc                                                                                                                       >  1:1027582/1‑150 (MQ=255)
                                 cAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCa                                                                                                                      >  2:1883342/1‑150 (MQ=255)
                                 cAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCa                                                                                                                      >  1:926653/1‑150 (MQ=255)
                                  aaaTATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCaa                                                                                                                     >  1:808449/1‑150 (MQ=255)
                                   aaTATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAg                                                                                                                    >  2:2003074/1‑150 (MQ=255)
                                     tataCGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGgc                                                                                                                  <  2:1586155/150‑1 (MQ=255)
                                     tataCGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGgc                                                                                                                  >  1:1804683/1‑150 (MQ=255)
                                       taCGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCACCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGgcgc                                                                                                                >  1:1457629/1‑150 (MQ=255)
                                        aCGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCa                                                                                                               <  2:623117/150‑1 (MQ=255)
                                        aCGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGGTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCa                                                                                                               <  1:379233/150‑1 (MQ=255)
                                         cGATAATGCAACCGGGCCAAAGGCAAGCGGTCCAGTCTTTCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAg                                                                                                              <  2:1941380/150‑1 (MQ=255)
                                         cGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGGCTCGGATTTGCTTAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCCTCTTGATCATCATTTTATTTCAAGGCGCAg                                                                                                              >  2:1269403/1‑150 (MQ=255)
                                          gATTATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAgg                                                                                                             <  2:128999/150‑1 (MQ=255)
                                            tAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGaa                                                                                                           <  1:168486/150‑1 (MQ=255)
                                             aaTGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAAc                                                                                                          <  2:1377556/150‑1 (MQ=255)
                                                      gggCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAatat                                                                                                 <  2:411985/150‑1 (MQ=255)
                                                       ggCCAAATGCAAGCGGTCCAGTCTTGCTCCAATTTGATCATATTGTTCCATTATGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATAtt                                                                                                <  2:368409/150‑1 (MQ=255)
                                                        gCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTg                                                                                               <  1:1316752/150‑1 (MQ=255)
                                                           aaaGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACa                                                                                            >  2:73997/1‑150 (MQ=255)
                                                                cAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACgtg                                                                                       >  2:744062/1‑150 (MQ=255)
                                                                 aaGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTATATTTCAAGGCGCAGGAACAAATAATATTGACAACgtgt                                                                                      <  1:1887598/150‑1 (MQ=255)
                                                                  aGCGGTCCAGTCTTGCGCCAATTTGATCTTATTGTTCCATTAGGTGCCTCGGATTTGCTAAATAGGTAGCAATTGCGTCAAATTCACTTTCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGtgtg                                                                                     <  2:1118814/150‑1 (MQ=255)
                                                                     ggTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCAGAATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAAAGTGTGCtt                                                                                  >  2:359542/1‑150 (MQ=255)
                                                                       tCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTaa                                                                                >  1:1766829/1‑150 (MQ=255)
                                                                       tCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTaa                                                                                >  1:526472/1‑150 (MQ=255)
                                                                         cAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAAt                                                                              >  1:1981054/1‑150 (MQ=255)
                                                                            tCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTAc                                                                           <  2:1031257/150‑1 (MQ=255)
                                                                            tCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTAc                                                                           <  1:1641059/150‑1 (MQ=255)
                                                                             attGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTAcc                                                                          <  2:807115/149‑1 (MQ=255)
                                                                               tGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACcac                                                                        <  2:546718/150‑1 (MQ=255)
                                                                                 cgcCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCacac                                                                      >  1:1986858/1‑150 (MQ=255)
                                                                                    cAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAg                                                                   <  1:498395/150‑1 (MQ=255)
                                                                                    cAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAg                                                                   <  1:1121604/150‑1 (MQ=255)
                                                                                      aTTTGATCATATTGTTCCATTTGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCt                                                                 >  1:1431643/1‑150 (MQ=255)
                                                                                       tttGATCATATTTTTCCATTAGGTGCCTAGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTg                                                                <  2:292170/150‑1 (MQ=255)
                                                                                       tttGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTg                                                                <  1:1598355/150‑1 (MQ=255)
                                                                                         tGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGa                                                              <  2:1667667/150‑1 (MQ=255)
                                                                                            tCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGatat                                                           <  1:1061712/150‑1 (MQ=255)
                                                                                                   gTTCCATTAGGTGCGTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACAACACCAGCTGGATATTATTGTc                                                    >  1:1394708/1‑150 (MQ=255)
                                                                                                   gTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTATATTTCAAGGCGCAGGAACAAATAATATTGACACCGTGTGCTTAAATTACCACACCAGCGGGATATTATTGtg                                                    >  2:143402/1‑149 (MQ=255)
                                                                                                       cATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTg                                                <  2:1838750/150‑1 (MQ=255)
                                                                                                        aTTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCTGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGc                                               <  1:809541/150‑1 (MQ=255)
                                                                                                          tAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCaa                                             >  1:2007046/1‑150 (MQ=255)
                                                                                                             gTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAAt                                          <  2:794545/150‑1 (MQ=255)
                                                                                                              ttccTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATg                                         <  1:1450484/148‑1 (MQ=255)
                                                                                                              tGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATg                                         <  1:1601154/150‑1 (MQ=255)
                                                                                                                    ggATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATcc                                   <  1:1883342/150‑1 (MQ=255)
                                                                                                                    ggATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATcc                                   <  1:1764668/150‑1 (MQ=255)
                                                                                                                      aTTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCAc                                 >  1:945898/1‑150 (MQ=255)
                                                                                                                           cTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGc                            >  2:848427/1‑150 (MQ=255)
                                                                                                                           cTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGc                            <  2:297580/150‑1 (MQ=255)
                                                                                                                            tAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCa                           >  1:580811/1‑150 (MQ=255)
                                                                                                                              aaaaGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATTTTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCAcc                         <  1:1808488/150‑1 (MQ=255)
                                                                                                                              aaaaGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTAACATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCAcc                         >  1:397206/1‑150 (MQ=255)
                                                                                                                                 aGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCgg                      <  1:1589188/150‑1 (MQ=255)
                                                                                                                                  ggTAGCAATTGCGGCAAATTCACTTGCCGGATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGt                     >  1:1228178/1‑150 (MQ=255)
                                                                                                                                  ggTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGt                     <  1:2003074/150‑1 (MQ=255)
                                                                                                                                    tAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGa                   <  2:808449/150‑1 (MQ=255)
                                                                                                                                        aaTTGCGGCAAATTCACTTGCCGCATCTTTATCATAATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTa               >  2:1679939/1‑150 (MQ=255)
                                                                                                                                           tGCGGCAAGTGAATTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCa            >  2:149365/1‑150 (MQ=255)
                                                                                                                                           tGCGGCAAGTGAATTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCa            >  1:987758/1‑150 (MQ=255)
                                                                                                                                           tGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCa            <  1:992046/150‑1 (MQ=255)
                                                                                                                                            gCGGCAAATTCAATTGCCGCATATTTATAATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACAAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTTTCAg           <  1:1214060/150‑1 (MQ=255)
                                                                                                                                             cGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCTGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGt          >  2:1501994/1‑150 (MQ=255)
                                                                                                                                              ggCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGtt         >  1:1889410/1‑150 (MQ=255)
                                                                                                                                              ggCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGtt         >  1:1248829/1‑150 (MQ=255)
                                                                                                                                               gCAAATTCACTTGCCGCATCTTTATCATAATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTTTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGtta        >  1:1899209/1‑150 (MQ=255)
                                                                                                                                                    ttCACTTGCCGCATCTGTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCCCCCGGTGATTTATCAGTTATTATg   >  2:356830/1‑150 (MQ=255)
                                                                                                                                                     tCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGTTATTATGt  <  1:744062/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGTTATTATGT  >  NC_000913/1853874‑1854172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: