Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,856,334 C→A 5.6% E199* (GAG→TAG)  ydjH ← putative kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,856,3340CA 5.6% 310.9 / 5.9 110E199* (GAG→TAG) ydjHputative kinase
Reads supporting (aligned to +/- strand):  ref base C (47/55);  new base A (2/5);  total (49/61)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 113 total aligned reads displayed.

TGCGACCGCCGGCACCTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTAC  >  NC_000913/1856185‑1856480
                                                                                                                                                     |                                                                                                                                                  
tGCGACCGCTGGCAATTTCTTGTTCATGTCACCAAGCATGATAAAGCAGCCGTCTTTAGCGGTTTTAATCACCACCGTTTTTTCGGCGTACGCAATTAAGCAGTCAGCAATTTCATCCTGTGTCTCTTTCCCTGTGAGTAATTTTGCCTc                                                                                                                                                    <  1:764218/150‑1 (MQ=255)
tGCGACCGCCGGCACCTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTc                                                                                                                                                    >  1:607878/1‑150 (MQ=255)
tGCGACCGCCGGCACCTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTc                                                                                                                                                    <  1:1545278/150‑1 (MQ=255)
 gCGACAGCCGGCACCTTCATCGTCATGTCACCACGCTTGATAAAGGAGCCGTCTTTACCCGTTTTAATCTCCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCg                                                                                                                                                   <  2:1412770/150‑1 (MQ=255)
  cGACCGCCGGCACCTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCgg                                                                                                                                                  <  1:1095244/150‑1 (MQ=255)
    aCCGCCGGCACCTTCATCGTCATGTCACCACGCATGATAAAGCAGCCGTCTTTACCCGTTTTAATCACAACCGTTTTTACGCCGCACGCAAGAAAGCCGGCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCCGCa                                                                                                                                                >  2:959608/1‑150 (MQ=255)
     ccgccgGCACCTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCaa                                                                                                                                               >  2:1999127/1‑150 (MQ=255)
       gccgGCACCTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCaaaa                                                                                                                                             >  2:1545578/1‑150 (MQ=255)
         cgGCACCTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCATTCCCGGTGAGTAATTTTGCCTCGGCAAAAtt                                                                                                                                           <  1:1204905/150‑1 (MQ=255)
           gCACCTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTac                                                                                                                                         <  1:1761894/150‑2 (MQ=255)
           gCACCTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTac                                                                                                                                         <  1:1623198/150‑2 (MQ=255)
            cACCTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTact                                                                                                                                        <  2:536034/150‑3 (MQ=255)
             aCCTTCATCGGCATGGCACCACGCTTGATAACGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCGCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTactc                                                                                                                                       <  1:1242342/150‑4 (MQ=255)
              ccTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCTCCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGaa                                                                                                                                      <  1:1942211/150‑1 (MQ=255)
                ttCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAAc                                                                                                                                    <  2:667242/150‑1 (MQ=255)
                ttCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAAc                                                                                                                                    <  1:1276538/150‑1 (MQ=255)
                ttCATCGTCATGTCACCACGCGTGATAAAGCAGCCGTCTTTACCCGTTTTCATCACCCCCGGTTTTACGCCGCACTCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGGGCGTAATTTTGCCTCGGCAAAATTAGGAAAc                                                                                                                                    <  2:1647736/150‑1 (MQ=255)
                 tCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACa                                                                                                                                   >  1:1523669/1‑150 (MQ=255)
                 tCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACa                                                                                                                                   >  1:735649/1‑150 (MQ=255)
                 tCATCGTCATGTCACCACGCTTGATAAAGCAGCAGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACa                                                                                                                                   <  2:142587/150‑1 (MQ=255)
                      gTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATaa                                                                                                                              >  1:1983745/1‑150 (MQ=255)
                         aTGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCTTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCg                                                                                                                           <  2:65067/150‑1 (MQ=255)
                         aTGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCg                                                                                                                           <  2:1845282/150‑1 (MQ=255)
                          tGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCTGTCAGCAATTTCATCCAGTGGCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGATACAGATAATCGa                                                                                                                          >  1:966251/1‑150 (MQ=255)
                          tGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGa                                                                                                                          >  1:997862/1‑150 (MQ=255)
                             caccacGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACAt                                                                                                                       <  2:1383694/150‑1 (MQ=255)
                              accacGCTTGATAAAGCAGCCGTCTTTAACCGTTTTAATCAACACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATa                                                                                                                      <  2:1893920/150‑1 (MQ=255)
                               ccacGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCCCCGTTTTTACGCCGCCCGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTACCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATaa                                                                                                                     >  1:1776665/1‑150 (MQ=255)
                                cacGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCCTCCAGTGTCTCTTTCCCGGTGAGTAATTATGCCTAGGCAAAATTAGGAAACAGATAATCGACATAAc                                                                                                                    >  1:1681735/1‑150 (MQ=255)
                                cacGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCAACGTTTTTACGCCTCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAAc                                                                                                                    <  1:1371569/150‑1 (MQ=255)
                                 acGCTTGATAAAGCAGCCGTCTTTACACGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACt                                                                                                                   >  1:1906883/1‑150 (MQ=255)
                                  cGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACta                                                                                                                  >  2:409360/1‑149 (MQ=255)
                                  cGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACta                                                                                                                  >  1:413998/1‑149 (MQ=255)
                                      tGATAAAGCAGCCGTCTTTAACCGTTTTAATCACCACCTTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATg                                                                                                              <  1:971846/150‑1 (MQ=255)
                                        aTAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCt                                                                                                            >  1:231373/1‑150 (MQ=255)
                                            agcagcCGTCTTTACCCGTTTTAATCACCCCCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGc                                                                                                        <  2:127453/150‑1 (MQ=255)
                                            agcagcCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGc                                                                                                        >  1:647622/1‑150 (MQ=255)
                                                gcCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAat                                                                                                    <  2:1438915/150‑1 (MQ=255)
                                                gcCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAat                                                                                                    <  1:1810646/150‑1 (MQ=255)
                                                 ccGTCTTTACCCGTTTTAATCACGACCGTTTTTACGCCTCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAACTAGGAAACAGATAATCGACATAACTCAATGCTGCGCAAata                                                                                                   <  1:1875046/150‑1 (MQ=255)
                                                 ccGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAata                                                                                                   <  1:1756855/150‑1 (MQ=255)
                                                      tttACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATatcatc                                                                                              <  1:697752/150‑1 (MQ=255)
                                                       ttACCAGTTTTAATCACCAACGTTTTTACGCCGCACGCTAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTGGCAAATATCATcc                                                                                             <  2:124641/150‑1 (MQ=255)
                                                        tACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCa                                                                                            >  2:267091/1‑150 (MQ=255)
                                                        tACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTACATCCAGTGTCTCTTTCCCGGTGACTAATTTTGCCTCTGCAAAATTAGGAAACAGATAAGCGACATACCTCAATGCTTCGCAAATATCATACa                                                                                            >  1:1230045/1‑150 (MQ=255)
                                                        tACCCGTTTTAATCACAACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATACa                                                                                            >  2:1361817/1‑150 (MQ=255)
                                                         aCCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGCGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAg                                                                                           >  1:915616/1‑150 (MQ=255)
                                                          cccGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCACTTTCATCCAGTGTCTCTTTCCCGGTGAGTAAATTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAATTATCATCCAGc                                                                                          >  1:1907463/1‑150 (MQ=255)
                                                          cccGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGc                                                                                          >  1:1092796/1‑150 (MQ=255)
                                                            cGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGt                                                                                        >  1:64833/1‑150 (MQ=255)
                                                               tttAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttc                                                                                     <  2:621303/150‑1 (MQ=255)
                                                                 tAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttcat                                                                                   >  2:1137489/1‑150 (MQ=255)
                                                                 tAATCACCACCGTTTTTACGCCGCACGAAAGAAAGAAGTCAGCAATTTCATCAAGTGTCTCTTTCCCGGTGAGTAAGTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttcat                                                                                   <  2:1507519/150‑1 (MQ=255)
                                                                        caccGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACcg                                                                            >  2:1198125/1‑150 (MQ=255)
                                                                         accGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACcgc                                                                           >  1:572719/1‑150 (MQ=255)
                                                                           cGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCgg                                                                         <  1:1108747/150‑1 (MQ=255)
                                                                           cGTTTTTACGCCGAACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGAAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCgg                                                                         <  2:275325/150‑1 (MQ=255)
                                                                              ttttACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGttt                                                                      <  1:1522647/150‑1 (MQ=255)
                                                                               tttACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTg                                                                     <  2:868010/150‑1 (MQ=255)
                                                                                  aCGCCGCACGCAAGAATGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGACTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATc                                                                  <  2:1255345/150‑1 (MQ=255)
                                                                                  aCGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATc                                                                  <  2:1664185/150‑1 (MQ=255)
                                                                                   cgccgcACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGCCATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCa                                                                 >  2:1722632/1‑150 (MQ=255)
                                                                                     ccgcACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCata                                                               >  1:254996/1‑150 (MQ=255)
                                                                                     ccgcACGCAAGAAAGCAGTCAGCAATTTCATCAAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCata                                                               <  1:1308457/150‑1 (MQ=255)
                                                                                      cgcacgcaAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCatat                                                              >  2:1141307/1‑150 (MQ=255)
                                                                                      cgcacgcaAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCatat                                                              <  2:1906883/150‑1 (MQ=255)
                                                                                       gcacgcaAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATc                                                             <  2:929171/150‑1 (MQ=255)
                                                                                            caAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAATATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCTAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGcac                                                        >  1:296585/1‑150 (MQ=255)
                                                                                            caAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATTCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGGTTGATCATATCGGcac                                                        >  1:1341553/1‑150 (MQ=255)
                                                                                            caAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGcac                                                        >  1:358161/1‑150 (MQ=255)
                                                                                              aGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAg                                                      >  1:1526011/1‑150 (MQ=255)
                                                                                                aaaGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCACTGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGGTTGATCATATCGGCACAGAt                                                    <  1:1698350/150‑1 (MQ=255)
                                                                                                  agaaGTCAGAAATTTAATCCTGTTTTTCTTTCCCGGTTTTTAATTTTGCCTCGCAAAAATTAGTAAACATATAATCTACATAACTCAATGCTTCGAAAATATCATCCAGGGTTTCATTCACCCGCGGTTTGATCATATCGGCACAGATaa                                                  <  2:1271287/147‑1 (MQ=255)
                                                                                                      gTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAatcat                                              >  1:673495/1‑150 (MQ=255)
                                                                                                         aGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTg                                           <  2:1159749/150‑1 (MQ=255)
                                                                                                         aGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTg                                           >  2:2001965/1‑150 (MQ=255)
                                                                                                         aGCAAATTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTg                                           <  1:300044/150‑1 (MQ=255)
                                                                                                             aTTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCgg                                       <  2:1551303/150‑1 (MQ=255)
                                                                                                                tCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCt                                    <  2:254996/150‑1 (MQ=255)
                                                                                                                 cATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCtt                                   <  2:930527/150‑1 (MQ=255)
                                                                                                                  aTCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCttt                                  >  1:1467727/1‑150 (MQ=255)
                                                                                                                   tCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTa                                 >  1:1151990/1‑150 (MQ=255)
                                                                                                                   accAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTa                                 <  1:228645/149‑1 (MQ=255)
                                                                                                                      aGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCt                              <  2:222516/150‑1 (MQ=255)
                                                                                                                      aGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGAAAATCATCTGCCGGGCTTTAGCt                              <  2:817958/150‑1 (MQ=255)
                                                                                                                          tctcTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCg                          <  2:1991870/150‑1 (MQ=255)
                                                                                                                          tctcTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCg                          >  1:1885833/1‑150 (MQ=255)
                                                                                                                           ctctTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCCTCCAGCGTTTCCTTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGt                         >  2:997179/1‑150 (MQ=255)
                                                                                                                             ctTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTaa                       >  1:1914191/1‑150 (MQ=255)
                                                                                                                                tCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTaaaaa                    <  2:1912878/150‑1 (MQ=255)
                                                                                                                                    ggTGAGTAATTTTGCCTCGGCAAAATTAGGTAACTGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTc                <  1:949374/150‑1 (MQ=255)
                                                                                                                                    ggTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCAAATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTc                <  1:228140/150‑1 (MQ=255)
                                                                                                                                    ggTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTGCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTc                <  2:861197/150‑1 (MQ=255)
                                                                                                                                        aGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGtt            >  1:794784/1‑150 (MQ=255)
                                                                                                                                         gTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTa           >  1:927325/1‑150 (MQ=255)
                                                                                                                                           aaTTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAgc         <  2:358161/150‑1 (MQ=255)
                                                                                                                                           aaTTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAgc         <  2:296585/150‑1 (MQ=255)
                                                                                                                                             ttttGCCTGGGCAAAATTAGGAAACAGATAATCGACATTACTCAATGCTTCGCATATATCATCCAGGGTTTCATTGAACCGGGGGTTGTTCATATCGGCACTGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAATTTTCTGTTAgcgc       <  1:1741767/150‑1 (MQ=255)
                                                                                                                                                 gCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTa   >  2:640542/1‑150 (MQ=255)
                                                                                                                                                  ccTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTAc  >  2:675086/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TGCGACCGCCGGCACCTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTAC  >  NC_000913/1856185‑1856480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑