Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,864,763 A→T 5.0% intergenic (+29/+19) yeaD → / ← yeaE D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,864,7630AT 5.0% 355.6 / 2.9 122intergenic (+29/+19)yeaD/yeaED‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base A (57/57);  new base T (2/6);  total (59/63)
Fisher's exact test for biased strand distribution p-value = 2.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

CCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGC  >  NC_000913/1864615‑1864910
                                                                                                                                                    |                                                                                                                                                   
 cGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCTAAGAGAAACCTGGACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATg                                                                                                                                                   <  1:64147/150‑1 (MQ=255)
 cGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATg                                                                                                                                                   <  2:1345710/150‑1 (MQ=255)
 cGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATg                                                                                                                                                   <  1:718725/150‑1 (MQ=255)
        tgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCgg                                                                                                                                            >  1:155837/1‑150 (MQ=255)
         ggatACAAAATATTTTTTTGTGTAGATAAGTCTTTCGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATGTGGCGCAATCCATTCGCGTTGCGAAACGTTATTTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGc                                                                                                                                           <  1:1226816/147‑1 (MQ=255)
         ggCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGATACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGGAATGAATCCGGc                                                                                                                                           >  1:1807685/1‑150 (MQ=255)
         ggCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATACGGc                                                                                                                                           >  1:590592/1‑150 (MQ=255)
         gccTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAATAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGGTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGc                                                                                                                                           <  2:303181/148‑1 (MQ=255)
           cTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTGCAGAACCGCAAAAAGTGACCAAAGAGAAACATGCACATCTGGCGCAATCCATTCGCGTTGCGAAACATTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcac                                                                                                                                         >  1:1379356/1‑150 (MQ=255)
            tACAAAACATATGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGcaca                                                                                                                                        <  1:1651369/150‑1 (MQ=255)
             aCAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcacac                                                                                                                                       <  1:1210032/150‑1 (MQ=255)
              cAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcacaca                                                                                                                                      >  2:843555/1‑150 (MQ=255)
              cAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcacaca                                                                                                                                      >  2:598775/1‑150 (MQ=255)
              cAAAACATTTGTGTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGGTGCGAAACGTAAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcacaca                                                                                                                                      <  1:1891666/150‑1 (MQ=255)
               aaaaCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGCCCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcacacca                                                                                                                                     >  2:1398988/1‑148 (MQ=255)
                aaaCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACacaac                                                                                                                                    >  2:1330153/1‑150 (MQ=255)
                    aTTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAACCCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATca                                                                                                                                >  2:2000717/1‑150 (MQ=255)
                     tttgtttgTGTATTAACGGCTTACGCTTCAGAAACGCAAAATGTGACCAAAGTGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAAGGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATcac                                                                                                                               <  2:108342/150‑1 (MQ=255)
                       tgtttttGTATAAACGGCTTAGTCTTCAGAAAGGTAAAAAGTCACCAAATAGAAACATGCACATCTGGGGCAATCAATTCGCTTTGGTAAACGTTAATTTACGTTAATGTTGTGGGCGGGGTGCATTGCATCCGGCACACAACATCacac                                                                                                                             <  2:1731219/150‑1 (MQ=255)
                       tgtttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATACGGCACACAACATCacac                                                                                                                             >  1:617727/1‑150 (MQ=255)
                        gtttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAAAATAacaca                                                                                                                            >  2:830048/1‑149 (MQ=255)
                              ttAGACACGGATTACGCTTCACAAACGAAAAAAGGCACCAAAGACAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCTAAACGTTAATTTACTTAAATGTTGTGTGCCGGGTGCTATGCATCCGGCACACAACATCACACCATATcc                                                                                                                      <  2:1963856/149‑1 (MQ=255)
                              gtAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAATCCTGCACATCTGGCGCAATCCATTCGCGTAGCGAAACGGTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCCCACCatttgc                                                                                                                      >  1:335344/1‑146 (MQ=255)
                              gtAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATcc                                                                                                                      >  2:716538/1‑150 (MQ=255)
                              gtAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGACGGGTGCAAATCATACGGCACACAACATCACCCCaaatac                                                                                                                      >  2:1496388/1‑148 (MQ=255)
                                 gAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAgc                                                                                                                   >  2:1152228/1‑150 (MQ=255)
                                   aaCGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAgcgc                                                                                                                 >  2:1403498/1‑150 (MQ=255)
                                   aaCGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAgcgc                                                                                                                 <  1:878209/150‑1 (MQ=255)
                                      ggCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAAAGTTAATTTACGTAAATGTTGTGTGCCGGGTGCAATGCAGCCCGCACCCAACAGCCCCCCAAATCCCGCGCAgg                                                                                                              >  2:1022754/1‑149 (MQ=255)
                                        cTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGttt                                                                                                            >  2:654547/1‑150 (MQ=255)
                                        cTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCAGGCGCAATCCATTCGCGTTGCGAAACGTTAATATACGTTAATCTTGTGTGCCCGGTGCAATGCATACGGCACCCAACATACCACCATATCCAGCGCAGtta                                                                                                            >  2:1925279/1‑149 (MQ=255)
                                         ttACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGtttt                                                                                                           <  1:994929/150‑1 (MQ=255)
                                           aCGCTTCAGAAACGCAATAAGTGACCAAAGAGAAACCTGCAGATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAAGGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTc                                                                                                         <  1:242373/150‑1 (MQ=255)
                                            cGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGAGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTcc                                                                                                        <  2:1316544/150‑1 (MQ=255)
                                            cGCTTAATAAACTCAAAAAGTCACCAACGAGAACCCTGCACATCGGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTGGTGGGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTcc                                                                                                        <  1:1828809/150‑1 (MQ=255)
                                               ttCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCttt                                                                                                     <  1:1374841/150‑1 (MQ=255)
                                                tCAGAAACGCAAAAAGTGAGCAAAGAGAAACCTGCACATCTGGCTCAATCCATTCGCGTTTCGAAACGTTAATTTACGTTAATGTTGTGTGCCTGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCtttt                                                                                                    <  1:1176745/150‑1 (MQ=255)
                                                tCAGAAACCAAAAACGTGACCAACTAGAAACCTGCACATCTGGCGCAATCATTTCGCGTTGCGAAACGTTAATTTACGTTAATGTGGTGGGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCtttt                                                                                                    <  2:1273483/150‑1 (MQ=255)
                                                 cAGAAACGCAAAAAGTGACCAACGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTg                                                                                                   <  2:315570/150‑1 (MQ=255)
                                                 cAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCTCAGTTTTTCCTTTTg                                                                                                   <  1:818053/150‑1 (MQ=255)
                                                 cAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTg                                                                                                   >  1:84077/1‑150 (MQ=255)
                                                 cAGAAACCCAAAAAGTGACCAAAGAGAAACCTCCACATCTGGCGCAATCCATTCGCGTGGCTAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTg                                                                                                   <  1:649185/150‑1 (MQ=255)
                                                   gAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAACCGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCCCACAACCTCCCCCCATATCCAGCGCAGGTTTTCCTGTTGGt                                                                                                 >  2:1282880/1‑150 (MQ=255)
                                                             aaGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTTCCGGGTGCAATGCATCCGGCACACAACATCACCCCATATCCAGAGCAGTTTTTCCTTTTGGTGCCGGATATg                                                                                       >  1:1387864/1‑150 (MQ=255)
                                                             aaGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATg                                                                                       <  1:108417/150‑1 (MQ=255)
                                                                tGACCAAAGAGAAACCTGCACATCGGGCGCAATCCATTCGCGTGGCGAAACGTTAATTTACGTTATTGTGGGGTGCCGGGGGCAAGGCAGCGGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCt                                                                                    <  1:927172/150‑1 (MQ=255)
                                                                  aCCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTa                                                                                  >  2:239592/1‑150 (MQ=255)
                                                                   ccAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCt                                                                                    >  1:579394/1‑147 (MQ=255)
                                                                   ccAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCt                                                                                    <  2:579394/147‑1 (MQ=255)
                                                                   ccAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATACGGCACCCAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTaa                                                                                 >  2:1833483/1‑149 (MQ=255)
                                                                    cAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATACAGAGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATc                                                                                >  2:1856024/1‑150 (MQ=255)
                                                                     aaaGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACCCCATATCCAGCGAAGTTTTTCCTTTTGGTGCCGGATAAGCCTTATcc                                                                               >  2:226510/1‑150 (MQ=255)
                                                                       agagAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTGGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGATTTTCCTTTTGGTGCCGGATATGCCTTATCCAg                                                                             <  1:301116/150‑1 (MQ=255)
                                                                          gaAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCAt                                                                          <  2:587936/150‑1 (MQ=255)
                                                                          gaAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATACAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCAt                                                                          >  1:1714764/1‑150 (MQ=255)
                                                                            aaCCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAg                                                                        <  1:1407332/150‑1 (MQ=255)
                                                                             aCCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGc                                                                       <  1:843555/150‑1 (MQ=255)
                                                                             aCCTGCACATCTGGCGAAATCAATTGGCGTTGCTAAACTTTAATTTACGTTAATGTGGTGTGCCGGTTGAAATGCATCCGGAACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGc                                                                       <  2:672674/150‑1 (MQ=255)
                                                                                tGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGTCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCTGGATAAGGCATATCCGGCaccaaaag                                                                    <  2:241114/150‑8 (MQ=255)
                                                                                tGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTaa                                                                    <  1:168172/150‑1 (MQ=255)
                                                                                 gCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGGCGGGTGCAATTCATACGGCACCCAACAACAAACAATATCCAGCGCAGTTTTTACTTTTTGTGCCGGATATTCCGTATCCCGCAAAGCAAAt                                                                   >  2:1667705/1‑150 (MQ=255)
                                                                                  cacaTCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGGCGGGTGCAATGCATCCGGCACACAACATACCACCATTTCCAGCGCGGTTTTTCCGTTGGGGGCCGGCTATGCGGTATCCAACATAGATAAtt                                                                  >  2:1847899/1‑150 (MQ=255)
                                                                                      tCTGGCGCAATCCATTCGCGTTGCGAAACTTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGc                                                              <  1:179168/150‑1 (MQ=255)
                                                                                        tGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATTTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTg                                                            <  2:252274/150‑1 (MQ=255)
                                                                                          gcgcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaag                                                         <  2:1049388/150‑1 (MQ=255)
                                                                                          gcgcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGACACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaa                                                          <  2:475287/150‑1 (MQ=255)
                                                                                           cgcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaag                                                         <  1:790651/150‑1 (MQ=255)
                                                                                            gcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGTTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaaga                                                        <  1:95691/150‑1 (MQ=255)
                                                                                            gcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTGGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaaga                                                        <  1:674125/150‑1 (MQ=255)
                                                                                               aTCCATTCGCTTTGCTAAACGTTAATTTACGTAAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAg                                                     <  1:1606596/150‑1 (MQ=255)
                                                                                                 ccATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGtt                                                   >  1:1173364/1‑150 (MQ=255)
                                                                                                   aTTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCg                                                 <  2:84077/150‑1 (MQ=255)
                                                                                                     tCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGAc                                               <  2:155837/150‑1 (MQ=255)
                                                                                                      cgcgTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGAATCCGGAACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGAcc                                              <  1:977068/150‑1 (MQ=255)
                                                                                                       gcgTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCt                                             <  2:75898/150‑1 (MQ=255)
                                                                                                         gTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACAATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCa                                           >  1:1191031/1‑150 (MQ=255)
                                                                                                            gCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGc                                        <  2:1060810/150‑1 (MQ=255)
                                                                                                            gCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATAACACCATATACAGCGCAGTTTTTACTTTTGGTGCCGGATATGCCTTATACAGCATAGCTAATTACGCTGAAGAAAGTTAGACCTCAAGc                                        >  1:401800/1‑150 (MQ=255)
                                                                                                              gAAACGTTAATTTACGTTAATGTTGTGTTCCGGGTGCAAAGCATCCGGCACACAACATCCCACCATATACAGCCGAGTTTTTCCTTTTGGTGCCGGATTTTCCGTATCCCGCATAGCTTATTCCCCTGAAGAAAGTTCGACCTCAACcac                                      >  2:646742/1‑150 (MQ=255)
                                                                                                                    ttAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGc                                <  2:945686/150‑1 (MQ=255)
                                                                                                                     tAATTTACGATATTGTTTTGTGCCGGGTGCATTGCATCCGGCCAACTACATCACACCATATCCATCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCa                               <  1:1508404/150‑1 (MQ=255)
                                                                                                                         ttACGTTAATGTTGTGTGCCGGGGGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCAtttt                           <  2:574499/150‑1 (MQ=255)
                                                                                                                           aCGTTATTGTTGTGGGCGGGGGCCAAGGAATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgt                         <  1:1496388/150‑1 (MQ=255)
                                                                                                                            cGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATACAGCGCAGGTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgtt                        >  1:1320551/1‑150 (MQ=255)
                                                                                                                             gTTATTGTTGTGTGCGGGGTGAATTGAATCGGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgttg                       <  2:1600884/150‑1 (MQ=255)
                                                                                                                             gTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgttg                       <  2:1898353/150‑1 (MQ=255)
                                                                                                                                   gttgtGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCCACCTCAATCACAGCCGCATTTTGTTGGACATg                 >  2:1431612/1‑150 (MQ=255)
                                                                                                                                     tgtgtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATggg               >  1:1165118/1‑150 (MQ=255)
                                                                                                                                     tgtgtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGGTCGACCTCAAGCACAGCCGCATTTTGTTGGACATggg               >  1:1557223/1‑150 (MQ=255)
                                                                                                                                     tgtgtgCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGTTATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGATATggg               >  2:1969958/1‑150 (MQ=255)
                                                                                                                                       tgtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGCAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCa             >  1:1139510/1‑150 (MQ=255)
                                                                                                                                       tgtgCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGCCCTCAAGCACAGCCGCATTTTGTTGGACAGGGGCa             >  1:1947981/1‑150 (MQ=255)
                                                                                                                                        gtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCaa            >  1:1052581/1‑150 (MQ=255)
                                                                                                                                          gCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATc          >  2:4555/1‑150 (MQ=255)
                                                                                                                                          gCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGAGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCGTAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATc          >  2:1558942/1‑150 (MQ=255)
                                                                                                                                            cGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGt        >  2:1091202/1‑150 (MQ=255)
                                                                                                                                            cGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGt        >  1:711450/1‑150 (MQ=255)
                                                                                                                                                 gCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCg   >  1:1507047/1‑150 (MQ=255)
                                                                                                                                                  cAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCgg  >  2:1649738/1‑149 (MQ=255)
                                                                                                                                                  cAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGc  >  2:504501/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGC  >  NC_000913/1864615‑1864910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: