Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,888,030 A→T 5.3% intergenic (‑39/+31) rnd ← / ← fadD ribonuclease D/acyl‑CoA synthetase (long‑chain‑fatty‑acid‑‑CoA ligase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,888,0300AT 5.3% 409.1 / 4.0 135intergenic (‑39/+31)rnd/fadDribonuclease D/acyl‑CoA synthetase (long‑chain‑fatty‑acid‑‑CoA ligase)
Reads supporting (aligned to +/- strand):  ref base A (69/58);  new base T (4/4);  total (73/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 137 total aligned reads displayed.

ATAAGTGCGCGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTATC  >  NC_000913/1887881‑1888179
                                                                                                                                                      |                                                                                                                                                     
ataaGTGCGCGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGa                                                                                                                                                       <  2:508683/150‑1 (MQ=255)
 taaGTGCGCGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGAt                                                                                                                                                      >  2:39479/1‑150 (MQ=255)
    gTGCGCGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTaa                                                                                                                                                   >  2:623291/1‑150 (MQ=255)
     ggcgcGTACGAACAAATTCAGTATCCAGGGCTCTCGCCGGAAAGGCACGGACGGCTGCACACAAAGAAGCCAGCGCATCGTCCGGGGGAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAAc                                                                                                                                                  <  1:1215531/149‑1 (MQ=255)
      gcgcGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAAcc                                                                                                                                                 >  2:1349277/1‑150 (MQ=255)
         cgTACGAACAAATTCAGTATCCACGGCTATCGCCGGAAACGCACGGACGGCTTCACACAAAGAACCCAGCGCATCGTCCGTGGTAAACATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGCCAAAAAAAACGCCGGATTAACCGGc                                                                                                                                              <  2:748072/150‑1 (MQ=255)
          gTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAGACGCCGGATTAACCGGCg                                                                                                                                             >  2:112352/1‑150 (MQ=255)
             cGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGTTAATCCGGCGttt                                                                                                                                          <  1:506092/150‑3 (MQ=255)
             cGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCt                                                                                                                                          >  2:1602656/1‑150 (MQ=255)
                 cAAATTCAGTATTCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTATgacg                                                                                                                                      >  2:1792612/1‑150 (MQ=255)
                  aaaTTCAGTATCCACGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGTTAATCCGGCGtttttttt                                                                                                                                     <  1:688607/150‑8 (MQ=255)
                     ttCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACgactg                                                                                                                                  >  2:1122339/1‑150 (MQ=255)
                       cAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACgactgac                                                                                                                                >  2:1579360/1‑150 (MQ=255)
                        cgTATCCCGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACgactgact                                                                                                                               <  2:445446/149‑1 (MQ=255)
                        aGTATCCAGGTCTATCGACGGAAAGGCACGGACGCCTTCACACAAAGAAGCCATCGCTGCGTCGGTGGTAATCATTTGGTACTTCAAATTTTTTTCTCTTTAGTGGGCGACAAAAAAAACGCCGGATAACCCGGCGTCTGACgactgact                                                                                                                               <  2:525930/150‑1 (MQ=255)
                        aGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTATTTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGAGTAACCGGCGTCTGACgactgact                                                                                                                               >  1:370814/1‑150 (MQ=255)
                        aGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACgactgact                                                                                                                               >  1:1758645/1‑150 (MQ=255)
                        aGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACgactgact                                                                                                                               >  1:2015921/1‑150 (MQ=255)
                            tCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAAc                                                                                                                           <  2:618664/150‑1 (MQ=255)
                            tCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAAc                                                                                                                           >  1:1098869/1‑150 (MQ=255)
                            accAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAAc                                                                                                                           <  1:678600/149‑1 (MQ=255)
                             ccAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACg                                                                                                                          >  1:1430722/1‑150 (MQ=255)
                              cAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGACGCCCGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGc                                                                                                                         >  2:1848579/1‑150 (MQ=255)
                              cAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGc                                                                                                                         <  2:827988/150‑1 (MQ=255)
                               agtgCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCt                                                                                                                        <  1:1029317/147‑1 (MQ=255)
                                gggCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTc                                                                                                                       <  1:224959/150‑1 (MQ=255)
                                       ctccGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCttt                                                                                                                <  2:416975/148‑1 (MQ=255)
                                       cGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCttt                                                                                                                >  2:265163/1‑150 (MQ=255)
                                            gAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTTACGACTGCCTTAACGCTCAGGCTTTATTGt                                                                                                           >  2:194414/1‑150 (MQ=255)
                                              aaGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGGCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTcc                                                                                                         <  2:902152/150‑1 (MQ=255)
                                                 gCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACt                                                                                                      >  1:1743196/1‑150 (MQ=255)
                                                   acggacggCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACttt                                                                                                    >  1:266915/1‑150 (MQ=255)
                                                    cggatggCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAACCGCCGGATTAACCGGCGTCTGACGCCTGACTTAACGCTCAGGCGTTATTGTCCACTTTGc                                                                                                  >  2:376047/1‑150 (MQ=255)
                                                    cggacggCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTg                                                                                                   >  1:1595312/1‑150 (MQ=255)
                                                      gacggCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGcc                                                                                                 >  1:1830126/1‑150 (MQ=255)
                                                        cggCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCcgc                                                                                               <  2:336512/150‑1 (MQ=255)
                                                          gCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCcgcgc                                                                                             <  2:1006844/150‑1 (MQ=255)
                                                          gCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCcgcgc                                                                                             >  1:1944649/1‑150 (MQ=255)
                                                             tCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACAGGCGTCTGACGACTGACTTAACGCGCAGGCTTTATTGTCCACTTTGACGCGCGCt                                                                                          >  2:1912492/1‑150 (MQ=255)
                                                              cacacaAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCGCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCtt                                                                                         >  2:1851712/1‑150 (MQ=255)
                                                                cacaAAGAAGCCAGCGCATCGTCTGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTc                                                                                        >  2:511769/1‑150 (MQ=255)
                                                                cacaAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTATTTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGCAATAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCg                                                                                       >  2:1236219/1‑150 (MQ=255)
                                                                cacaAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGCTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCg                                                                                       >  2:638933/1‑150 (MQ=255)
                                                                     agaagCCAGCGCATCGTCCGTGGTAATCATTTGGAAATTCAATTTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACg                                                                                  <  2:1895421/150‑1 (MQ=255)
                                                                      gaagCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGt                                                                                 >  1:646899/1‑150 (MQ=255)
                                                                            aGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATtct                                                                           >  2:1872572/1‑150 (MQ=255)
                                                                               gcATCGTGCGTGGTAATTATTTGGTTATTCAAATTGTTTTCTCTTTAGTGGGGGTCAATTAAAACGCCGTTTTAACCGTCGTCTGAGGACTGACTTAACGCTCAGGCTTTATTGTCCAGTTTGCCGCGCGCTTCGTCACGTAATtctctt                                                                        <  2:714920/150‑3 (MQ=255)
                                                                               gcATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCtcgt                                                                        <  2:1047133/150‑1 (MQ=255)
                                                                                cATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCtcgtc                                                                       <  1:1147163/150‑1 (MQ=255)
                                                                                 aTCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCtcgtcg                                                                      <  2:1133381/150‑1 (MQ=255)
                                                                                  tCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGc                                                                     <  2:902676/150‑1 (MQ=255)
                                                                                       cGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAAt                                                                <  1:1887281/150‑1 (MQ=255)
                                                                                          ggTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGCCGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGTGCGCTTCGTCACGTAATTCTCGTCGCAAAAtttt                                                             >  1:1100942/1‑150 (MQ=255)
                                                                                           gTAATCTTTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTAAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAAttttt                                                            <  1:1023810/150‑1 (MQ=255)
                                                                                           gTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGTGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAAttttt                                                            <  1:1664862/150‑1 (MQ=255)
                                                                                               tcttttGGTAATTCAATTTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTTTTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGa                                                        <  2:670396/147‑1 (MQ=255)
                                                                                                 aTTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACg                                                      >  1:423190/1‑150 (MQ=255)
                                                                                                 aTTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACAGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCAGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACg                                                      >  1:122233/1‑150 (MQ=255)
                                                                                                      gTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGa                                                 <  2:1979286/150‑1 (MQ=255)
                                                                                                          ttCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTc                                             <  1:1967550/150‑1 (MQ=255)
                                                                                                           tCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCg                                            <  2:1693387/150‑1 (MQ=255)
                                                                                                            ctaaTTGTTTTCTCTTTAGTGGGCGTCAAAATAAACGCCGGATTAACCGGCGTCTGACGAATGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGACACCTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCgg                                           <  1:1199591/148‑1 (MQ=255)
                                                                                                            cAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGCCTTAACGCTCAGGCGTTATTGTCCACTTTGCCGCGCGCGTCGTCACGTAATTCTCGTCGCAAAAGTTTTCCGACGTTAGATTTCgg                                           >  2:1471107/1‑150 (MQ=255)
                                                                                                            cAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCgg                                           <  1:1954237/150‑1 (MQ=255)
                                                                                                            cAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGCCTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGt                                          >  2:848945/1‑150 (MQ=255)
                                                                                                            aaaaTTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCgg                                           <  2:1838255/149‑1 (MQ=255)
                                                                                                             aaaTTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGAATGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGAAGTTAGATTTCGGt                                          >  1:1002810/1‑150 (MQ=255)
                                                                                                                ttGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCTCTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCTGTAAc                                       <  2:1735239/150‑1 (MQ=255)
                                                                                                                ttGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAAc                                       <  1:265163/150‑1 (MQ=255)
                                                                                                                  gTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACtc                                     >  2:1032026/1‑150 (MQ=255)
                                                                                                                   ttttCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTACCGCTCAGGCTTTATTGGCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACtca                                    <  2:1884835/150‑1 (MQ=255)
                                                                                                                     ttCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACtcata                                  >  2:874471/1‑149 (MQ=255)
                                                                                                                      tctcTTTAGTGGGCGTCAAAAATAACGCGGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACtcatca                                 <  2:642282/150‑1 (MQ=255)
                                                                                                                      tctcTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACtcatca                                 <  1:1349277/150‑1 (MQ=255)
                                                                                                                       ctctTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCAc                                <  1:1665356/150‑1 (MQ=255)
                                                                                                                       ctctTTAGTGGGCGGCAAAAAAAACGCCGGATTAAGCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCAc                                <  1:864247/150‑1 (MQ=255)
                                                                                                                        tctTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCGTAACg                               >  2:1329485/1‑150 (MQ=255)
                                                                                                                          tttAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGaa                             <  1:1579360/150‑1 (MQ=255)
                                                                                                                            tAGTGGTCGTGAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAAc                           <  1:1701494/150‑1 (MQ=255)
                                                                                                                              gTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCGTCGTCACATAATTCTCGTCGCGAAATTTTTCCGACGTTAGAGTTCGGGAACTCATCCCGAAACTc                         >  2:538172/1‑150 (MQ=255)
                                                                                                                               tGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTcc                        >  1:1223426/1‑150 (MQ=255)
                                                                                                                                gggCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGGAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTcca                       >  1:1373047/1‑150 (MQ=255)
                                                                                                                                  gCGTCAAAAAATACGCCGGAGTAACCGTCGTCTGACGTCTGACTTAACGCTCAGGCTTTATTTTGCACTTTGCCGCGCGCTTCGTCACGTAAGTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAAGTCATCACGATACTccacc                     <  1:314495/150‑1 (MQ=255)
                                                                                                                                   cGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTccacca                    <  2:214219/150‑1 (MQ=255)
                                                                                                                                      cAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCt                 >  2:1689750/1‑150 (MQ=255)
                                                                                                                                        aaaaaaaaCGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCtt                >  1:68948/1‑150 (MQ=255)
                                                                                                                                          gtcagaCGCCGGTTAATCCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCg              >  2:1658824/6‑150 (MQ=255)
                                                                                                                                            aaaaCGCCGGATTAACCGGCGTCTTACGTCTGACTTAACGCTCAGTCTTTATTGTCCACTTTGGCGCGCGCATATTCACGTAATTCTCGTCGCAAAATTTTTCCGAGGTTAGATTTCGGTAACTCATCACGAAACTCCAGCAGCTTCGGt            <  2:160674/150‑1 (MQ=255)
                                                                                                                                            aaaaCGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGt            >  2:1793794/1‑150 (MQ=255)
                                                                                                                                             aaaCGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTa           >  1:886645/1‑150 (MQ=255)
                                                                                                                                              gaCGCCGGTTAATCCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTAc          >  1:1518179/2‑150 (MQ=255)
                                                                                                                                                cGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACtt        >  1:1571776/1‑150 (MQ=255)
                                                                                                                                                 gCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACttt       <  1:1354534/150‑1 (MQ=255)
                                                                                                                                                  ccGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCCCGAAACTCCACCAGCTTCGGTACTTTg      >  1:1184412/1‑150 (MQ=255)
                                                                                                                                                  ccGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTg      <  2:370814/150‑1 (MQ=255)
                                                                                                                                                  ccGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGCCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTg      <  2:1743196/150‑1 (MQ=255)
                                                                                                                                                   cGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCCCGAAACTCCACCAGCTTCGGTACTTTGt     >  1:205977/1‑150 (MQ=255)
                                                                                                                                                   cGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGt     >  1:1871391/1‑150 (MQ=255)
                                                                                                                                                    ggATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTa    >  1:521005/1‑150 (MQ=255)
                                                                                                                                                    ggATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTa    <  2:1430722/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
ATAAGTGCGCGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTATC  >  NC_000913/1887881‑1888179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: