Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,888,032 T→A 5.4% intergenic (‑41/+29) rnd ← / ← fadD ribonuclease D/acyl‑CoA synthetase (long‑chain‑fatty‑acid‑‑CoA ligase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,888,0320TA 5.4% 399.5 / 6.7 135intergenic (‑41/+29)rnd/fadDribonuclease D/acyl‑CoA synthetase (long‑chain‑fatty‑acid‑‑CoA ligase)
Reads supporting (aligned to +/- strand):  ref base T (68/59);  new base A (4/4);  total (72/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 136 total aligned reads displayed.

GTGCGCGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTATCC  >  NC_000913/1887885‑1888180
                                                                                                                                                    |                                                                                                                                                    
gTGCGCGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTaa                                                                                                                                                    >  2:1838532/1‑150 (MQ=255)
 ggcgcGTACGAACAAATTCAGTATCCAGGGCTCTCGCCGGAAAGGCACGGACGGCTGCACACAAAGAAGCCAGCGCATCGTCCGGGGGAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAAc                                                                                                                                                   <  1:1215531/149‑1 (MQ=255)
  gcgcGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAAcc                                                                                                                                                  >  2:1349277/1‑150 (MQ=255)
     cgTACGAACAAATTCAGTATCCACGGCTATCGCCGGAAACGCACGGACGGCTTCACACAAAGAACCCAGCGCATCGTCCGTGGTAAACATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGCCAAAAAAAACGCCGGATTAACCGGc                                                                                                                                               <  2:748072/150‑1 (MQ=255)
      gTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAGACGCCGGATTAACCGGCg                                                                                                                                              >  2:112352/1‑150 (MQ=255)
        aCGAACAAATTCAGTATCAAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGTTAATCCGGCGtt                                                                                                                                            <  2:352880/150‑2 (MQ=255)
         cGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCt                                                                                                                                           >  2:1602656/1‑150 (MQ=255)
         cGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATACAAATTGTGTTCTCTTTAGTGGGCGTCAAAAAAACCGCCGGATTAACCGGCGTCt                                                                                                                                           <  1:316700/150‑1 (MQ=255)
              aaaTTCAGTATCCACGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGTTAATCCGGCGtttttttt                                                                                                                                      <  1:688607/150‑8 (MQ=255)
                 ttCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACgactg                                                                                                                                   >  2:1122339/1‑150 (MQ=255)
                   cAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACgactgac                                                                                                                                 <  2:2007962/150‑1 (MQ=255)
                    aGTATCCAGGTCTATCGACGGAAAGGCACGGACGCCTTCACACAAAGAAGCCATCGCTGCGTCGGTGGTAATCATTTGGTACTTCAAATTTTTTTCTCTTTAGTGGGCGACAAAAAAAACGCCGGATAACCCGGCGTCTGACgactgact                                                                                                                                <  2:525930/150‑1 (MQ=255)
                    aGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACgactgact                                                                                                                                >  1:1758645/1‑150 (MQ=255)
                        tCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAAc                                                                                                                            <  2:618664/150‑1 (MQ=255)
                        tCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAAc                                                                                                                            >  1:1098869/1‑150 (MQ=255)
                        accAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAAc                                                                                                                            <  1:678600/149‑1 (MQ=255)
                         ccAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACg                                                                                                                           >  1:1430722/1‑150 (MQ=255)
                          cAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGc                                                                                                                          <  2:827988/150‑1 (MQ=255)
                          cAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGAATTAACGc                                                                                                                          >  2:394823/1‑150 (MQ=255)
                           agtgCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCt                                                                                                                         <  1:1029317/147‑1 (MQ=255)
                            gggCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTc                                                                                                                        <  1:224959/150‑1 (MQ=255)
                                   ctccGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCttt                                                                                                                 <  2:416975/148‑1 (MQ=255)
                                   cGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCttt                                                                                                                 >  2:265163/1‑150 (MQ=255)
                                        gAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTTACGACTGCCTTAACGCTCAGGCTTTATTGt                                                                                                            >  2:194414/1‑150 (MQ=255)
                                          aaGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGGCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTcc                                                                                                          <  2:902152/150‑1 (MQ=255)
                                           aGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGCCTGACTTAACGCTCAGGCTTTATTGTACa                                                                                                         >  2:1896391/1‑150 (MQ=255)
                                             gCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACt                                                                                                       <  2:629265/150‑1 (MQ=255)
                                               acggacggCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACttt                                                                                                     >  1:266915/1‑150 (MQ=255)
                                                cggatggCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAACCGCCGGATTAACCGGCGTCTGACGCCTGACTTAACGCTCAGGCGTTATTGTCCACTTTGc                                                                                                   >  2:376047/1‑150 (MQ=255)
                                                cggacggCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTg                                                                                                    >  1:1595312/1‑150 (MQ=255)
                                                  gacggCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGcc                                                                                                  >  1:1830126/1‑150 (MQ=255)
                                                    cggCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCcgc                                                                                                <  2:336512/150‑1 (MQ=255)
                                                    cggCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCcgc                                                                                                <  2:1751516/150‑1 (MQ=255)
                                                      gCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCcgcgc                                                                                              >  1:1944649/1‑150 (MQ=255)
                                                          cacacaAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCGCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCtt                                                                                          >  2:1851712/1‑150 (MQ=255)
                                                           acacaAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTc                                                                                         >  1:1979286/1‑150 (MQ=255)
                                                            cacaAAGAAGCCAGCGCATCGTCTGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTc                                                                                         >  2:511769/1‑150 (MQ=255)
                                                            cacaAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTATTTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGCAATAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCg                                                                                        >  2:1236219/1‑150 (MQ=255)
                                                            cacaAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGCTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCg                                                                                        >  2:638933/1‑150 (MQ=255)
                                                              caAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTc                                                                                      >  1:230644/1‑150 (MQ=255)
                                                                 agaagCCAGCGCATCGTCCGTGGTAATCATTTGGAAATTCAATTTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACg                                                                                   <  2:1895421/150‑1 (MQ=255)
                                                                  gaagCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGt                                                                                  >  1:646899/1‑150 (MQ=255)
                                                                        aGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATtct                                                                            >  2:1872572/1‑150 (MQ=255)
                                                                           gcATCGTGCGTGGTAATTATTTGGTTATTCAAATTGTTTTCTCTTTAGTGGGGGTCAATTAAAACGCCGTTTTAACCGTCGTCTGAGGACTGACTTAACGCTCAGGCTTTATTGTCCAGTTTGCCGCGCGCTTCGTCACGTAATtctctt                                                                         <  2:714920/150‑3 (MQ=255)
                                                                           gcATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCtcgt                                                                         <  2:1047133/150‑1 (MQ=255)
                                                                            cATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCtcgtc                                                                        <  1:1147163/150‑1 (MQ=255)
                                                                             aTCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCtcgtcg                                                                       <  2:1133381/150‑1 (MQ=255)
                                                                              tCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGc                                                                      <  2:902676/150‑1 (MQ=255)
                                                                                   cGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAAt                                                                 <  1:1887281/150‑1 (MQ=255)
                                                                                      ggTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGCCGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGTGCGCTTCGTCACGTAATTCTCGTCGCAAAAtttt                                                              >  1:1100942/1‑150 (MQ=255)
                                                                                       gTAATCTTTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTAAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAAttttt                                                             <  1:1023810/150‑1 (MQ=255)
                                                                                           tcttttGGTAATTCAATTTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTTTTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGa                                                         <  2:670396/147‑1 (MQ=255)
                                                                                            cATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGAc                                                        >  1:1228504/1‑150 (MQ=255)
                                                                                             aTTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACg                                                       >  1:423190/1‑150 (MQ=255)
                                                                                             aTTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACAGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCAGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACg                                                       >  1:122233/1‑150 (MQ=255)
                                                                                                  gTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGa                                                  <  2:1979286/150‑1 (MQ=255)
                                                                                                   tAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGGAAAAATTTTGCGACgagaatt                                                 <  2:1477984/150‑7 (MQ=255)
                                                                                                      ttCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTc                                              <  1:1967550/150‑1 (MQ=255)
                                                                                                       tCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCg                                             <  2:1693387/150‑1 (MQ=255)
                                                                                                        ctaaTTGTTTTCTCTTTAGTGGGCGTCAAAATAAACGCCGGATTAACCGGCGTCTGACGAATGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGACACCTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCgg                                            <  1:1199591/148‑1 (MQ=255)
                                                                                                        cAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGCCTTAACGCTCAGGCGTTATTGTCCACTTTGCCGCGCGCGTCGTCACGTAATTCTCGTCGCAAAAGTTTTCCGACGTTAGATTTCgg                                            >  2:1471107/1‑150 (MQ=255)
                                                                                                        cAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCgg                                            <  1:1602656/150‑1 (MQ=255)
                                                                                                        cAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCgg                                            <  1:1954237/150‑1 (MQ=255)
                                                                                                        cAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGCCTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGt                                           >  2:848945/1‑150 (MQ=255)
                                                                                                        aaaaTTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCgg                                            <  2:1838255/149‑1 (MQ=255)
                                                                                                         aaaTTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGAATGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGAAGTTAGATTTCGGt                                           >  1:1002810/1‑150 (MQ=255)
                                                                                                            ttGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCTCTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCTGTAAc                                        <  2:1735239/150‑1 (MQ=255)
                                                                                                              gTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACtc                                      >  2:1032026/1‑150 (MQ=255)
                                                                                                                 ttCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACtcata                                   >  2:874471/1‑149 (MQ=255)
                                                                                                                  tctcTTTAGTGGGCGTCAAAAATAACGCGGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACtcatca                                  <  2:642282/150‑1 (MQ=255)
                                                                                                                   ctctTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCAc                                 <  1:1665356/150‑1 (MQ=255)
                                                                                                                   ctctTTAGTGGGCGGCAAAAAAAACGCCGGATTAAGCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCAc                                 <  1:864247/150‑1 (MQ=255)
                                                                                                                    tctTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCGTAACg                                >  2:1329485/1‑150 (MQ=255)
                                                                                                                      tttAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGaa                              <  1:1579360/150‑1 (MQ=255)
                                                                                                                        tAGTGGTCGTGAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAAc                            <  1:1701494/150‑1 (MQ=255)
                                                                                                                          gTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCGTCGTCACATAATTCTCGTCGCGAAATTTTTCCGACGTTAGAGTTCGGGAACTCATCCCGAAACTc                          >  2:538172/1‑150 (MQ=255)
                                                                                                                           tGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTcc                         >  1:1223426/1‑150 (MQ=255)
                                                                                                                            gggCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGGAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTcca                        >  1:1373047/1‑150 (MQ=255)
                                                                                                                              gCGTCAAAAAATACGCCGGAGTAACCGTCGTCTGACGTCTGACTTAACGCTCAGGCTTTATTTTGCACTTTGCCGCGCGCTTCGTCACGTAAGTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAAGTCATCACGATACTccacc                      <  1:314495/150‑1 (MQ=255)
                                                                                                                                 tCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGc                   >  1:470875/1‑150 (MQ=255)
                                                                                                                                  cAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCt                  >  2:1689750/1‑150 (MQ=255)
                                                                                                                                    aaaaaaaaCGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCtt                 >  1:68948/1‑150 (MQ=255)
                                                                                                                                     cgtcagaCGCCGGTTAATCCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTc                >  1:1723489/7‑150 (MQ=255)
                                                                                                                                        aaaaCGCCGGATTAACCGGCGTCTTACGTCTGACTTAACGCTCAGTCTTTATTGTCCACTTTGGCGCGCGCATATTCACGTAATTCTCGTCGCAAAATTTTTCCGAGGTTAGATTTCGGTAACTCATCACGAAACTCCAGCAGCTTCGGt             <  2:160674/150‑1 (MQ=255)
                                                                                                                                        aaaaCGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGt             >  2:1793794/1‑150 (MQ=255)
                                                                                                                                         aaaCGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTa            >  1:886645/1‑150 (MQ=255)
                                                                                                                                          gaCGCCGGTTAATCCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTAc           >  2:47404/2‑150 (MQ=255)
                                                                                                                                          gaCGCCGGTTAATCCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTAc           >  1:1518179/2‑150 (MQ=255)
                                                                                                                                            cGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACtt         >  1:1571776/1‑150 (MQ=255)
                                                                                                                                            cGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACtt         >  2:188190/1‑150 (MQ=255)
                                                                                                                                             gCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACttt        <  1:1354534/150‑1 (MQ=255)
                                                                                                                                             gCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACttt        <  1:989966/150‑1 (MQ=255)
                                                                                                                                              ccGGATTAACCGGCGTCTGCCGCCTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGAATAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACGTTg       >  2:1637246/1‑150 (MQ=255)
                                                                                                                                              ccGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCCCGAAACTCCACCAGCTTCGGTACTTTg       >  1:1184412/1‑150 (MQ=255)
                                                                                                                                              ccGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGCCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTg       <  2:1743196/150‑1 (MQ=255)
                                                                                                                                               cGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCCCGAAACTCCACCAGCTTCGGTACTTTGt      >  1:205977/1‑150 (MQ=255)
                                                                                                                                               cGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGt      >  1:1871391/1‑150 (MQ=255)
                                                                                                                                                ggATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTa     >  1:521005/1‑150 (MQ=255)
                                                                                                                                                ggATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTa     <  2:1430722/150‑1 (MQ=255)
                                                                                                                                                   ttAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTATcc  <  1:638933/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GTGCGCGTACGAACAAATTCAGTATCCAGGGCTATCGCCGGAAAGGCACGGACGGCTTCACACAAAGAAGCCAGCGCATCGTCCGTGGTAATCATTTGGTAATTCAAATTGTTTTCTCTTTAGTGGGCGTCAAAAAAAACGCCGGATTAACCGGCGTCTGACGACTGACTTAACGCTCAGGCTTTATTGTCCACTTTGCCGCGCGCTTCGTCACGTAATTCTCGTCGCAAAATTTTTCCGACGTTAGATTTCGGTAACTCATCACGAAACTCCACCAGCTTCGGTACTTTGTATCC  >  NC_000913/1887885‑1888180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: