Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,889,917 C→G 5.8% intergenic (‑171/+34) fadD ← / ← yeaY acyl‑CoA synthetase (long‑chain‑fatty‑acid‑‑CoA ligase)/Slp family lipoprotein, RpoE‑regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,889,9170CG 5.8% 420.4 / 13.6 138intergenic (‑171/+34)fadD/yeaYacyl‑CoA synthetase (long‑chain‑fatty‑acid‑‑CoA ligase)/Slp family lipoprotein, RpoE‑regulated
Reads supporting (aligned to +/- strand):  ref base C (64/65);  new base G (2/6);  total (67/71)
Fisher's exact test for biased strand distribution p-value = 2.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.54e-01

Only 100 of 139 total aligned reads displayed.

GTTCGTCGTCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAG  >  NC_000913/1889768‑1890063
                                                                                                                                                     |                                                                                                                                                  
gtTCGTCGTCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGTAGAAACAGCGGAcc                                                                                                                                                    <  1:1898388/150‑1 (MQ=255)
    gtcgtcATCGCAACCCCTAATGATATACATGCCGTTAACATAATCTTAACTCCTCATACCACCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTCCCGTAAGACTGCAGAAACAGCGGACCAGcc                                                                                                                                                <  2:777670/150‑1 (MQ=255)
      cgtcATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGc                                                                                                                                              >  2:211515/1‑150 (MQ=255)
       gtcATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCt                                                                                                                                             >  2:464696/1‑150 (MQ=255)
       gtcATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCt                                                                                                                                             <  2:1445800/150‑1 (MQ=255)
       gtcATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCt                                                                                                                                             >  2:1091844/1‑150 (MQ=255)
        tcATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTg                                                                                                                                            >  2:25895/1‑150 (MQ=255)
            cGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCATGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttc                                                                                                                                        <  2:745630/150‑1 (MQ=255)
             gCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttct                                                                                                                                       <  1:1956778/150‑1 (MQ=255)
              gaaCCCCAAATGATATACATGCCGTTAACATAATTTTAACTCATCATAACAGCTTGATAATTACCCAACGAAAAGGTTGCTAAGCGCGTCACTATTTATTTTTATCTTTAGCGTAAGAATGCAGAAACAGTGGCTGGTCCGCTGtttctg                                                                                                                                      <  2:125311/149‑2 (MQ=255)
              cAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGCTGGTCCGCTGtttctg                                                                                                                                      <  2:855227/150‑2 (MQ=255)
              cAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGCTGGTCCGCTGtttctg                                                                                                                                      <  1:708726/150‑2 (MQ=255)
              cAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttctt                                                                                                                                      >  2:1266359/1‑150 (MQ=255)
              cAACCCCAAATGAAATACATGCCGATAACAAAATATTAACTCATCATACCCGCTTGATAATTACCCAACGAACAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTATCCCGTAAGAATGCAGAAACAGCGGCTGGTCCGCTGtttctg                                                                                                                                      <  2:912358/150‑2 (MQ=255)
               aaCCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttcttt                                                                                                                                     <  2:1408167/150‑1 (MQ=255)
                 ccccAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGCTGGTCCGCTGtttctgcat                                                                                                                                   <  2:551987/150‑5 (MQ=255)
                 ccccAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttcttttt                                                                                                                                   >  2:1679777/1‑150 (MQ=255)
                  cccAAATGATTTACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttctttttc                                                                                                                                  >  2:1144416/1‑150 (MQ=255)
                  cccAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttctttttg                                                                                                                                  >  1:473845/1‑149 (MQ=255)
                  cccAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttctttttc                                                                                                                                  >  2:1136178/1‑150 (MQ=255)
                   ccAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttctttttct                                                                                                                                 <  1:915779/150‑1 (MQ=255)
                    cAAATGATATACATGCCGTTAACAAAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttctttttctt                                                                                                                                <  1:139082/150‑1 (MQ=255)
                      aaTGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTa                                                                                                                              <  1:1518349/150‑1 (MQ=255)
                         gATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTaaaa                                                                                                                           <  2:1594274/150‑1 (MQ=255)
                            ataCATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTATTTAAAAACa                                                                                                                        >  1:1956509/1‑150 (MQ=255)
                               cATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttc                                                                                                                                        >  2:1050642/1‑131 (MQ=255)
                               cATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGtttc                                                                                                                                        <  1:1050642/131‑1 (MQ=255)
                                    cGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAatta                                                                                                                <  2:250083/150‑1 (MQ=255)
                                     gTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAattat                                                                                                               <  1:486402/150‑1 (MQ=255)
                                       tAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTc                                                                                                             <  2:552764/150‑1 (MQ=255)
                                         aCATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAg                                                                                                           <  1:1362863/150‑1 (MQ=255)
                                         aCATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAg                                                                                                           <  1:1200494/150‑1 (MQ=255)
                                          cATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAAAAAGCAATTATTCAGt                                                                                                          >  1:188153/1‑150 (MQ=255)
                                              atatTAACTCATCCTACCAGCGTGCTAATTACCCACCGAATAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTGCTTTAAAAACAAGCAATTATTCAGTTACa                                                                                                      <  2:1885804/150‑1 (MQ=255)
                                              atatTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTACTTTAAAAACAAGCAATTATTCAGTTACa                                                                                                      <  2:1878036/150‑1 (MQ=255)
                                                atTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAAc                                                                                                    >  1:1341249/1‑150 (MQ=255)
                                                atTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTAACaaa                                                                                                    >  1:1541653/1‑149 (MQ=255)
                                                    aCTCATCATACCAGCTTGATAATTACCCACCGAAAAGGTTCCGAAGCGCGTCACTATTTATTTTTATCTTTACAGTAAGAATGCAGAAACAGCGGACCAGCCTCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGtt                                                                                                <  2:362139/150‑1 (MQ=255)
                                                     cTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGttt                                                                                               <  2:1184974/150‑1 (MQ=255)
                                                      tcatcaTACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGtttt                                                                                              >  1:550474/1‑149 (MQ=255)
                                                       catcatACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGAGGACCAGCCGCTGGTTCTTATTATTTTAAAAAAAGCAATTATTCAGTTACCACTGTTTGt                                                                                             >  1:928363/1‑150 (MQ=255)
                                                        atcatACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTa                                                                                            <  1:1837209/150‑1 (MQ=255)
                                                          catACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTAct                                                                                          >  1:1328545/1‑150 (MQ=255)
                                                               cAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTcgc                                                                                     >  2:1536581/1‑150 (MQ=255)
                                                                aGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTcgcg                                                                                    <  2:179442/150‑1 (MQ=255)
                                                                 gCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGCCCAGCCGCTGTTTCTTTTTCTTTAAACAAAAGCAATTATTCAGTATCAACTGTTAGTACTCTCTCgg                                                                                   >  1:313441/1‑150 (MQ=255)
                                                                    tGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGc                                                                                >  1:433187/1‑150 (MQ=255)
                                                                      aTAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCg                                                                              <  1:140805/150‑1 (MQ=255)
                                                                       tAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCgg                                                                             >  1:1874640/1‑150 (MQ=255)
                                                                        aaTTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGAGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCggg                                                                            <  1:1653094/150‑1 (MQ=255)
                                                                        aaTTACCCAACGAAAAGGTTGCGAAGCGCGGCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGGTGTTTCTTTTTCTTTACAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCggg                                                                            <  2:230696/150‑1 (MQ=255)
                                                                         aTTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGa                                                                           >  2:1597209/1‑150 (MQ=255)
                                                                         aTTACCCAACGAAAAGGATGCGTAGCGCGTCACTATTTATTTTTATCTTTACCGCAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGa                                                                           <  2:128964/150‑1 (MQ=255)
                                                                          ttACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGAt                                                                          <  1:1550954/150‑1 (MQ=255)
                                                                           tACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGAtt                                                                         <  1:309036/150‑1 (MQ=255)
                                                                               cAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGCCCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATAc                                                                     >  2:1207905/1‑150 (MQ=255)
                                                                               cAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATAc                                                                     <  1:1266359/150‑1 (MQ=255)
                                                                                 aCGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCa                                                                   <  1:1666397/150‑1 (MQ=255)
                                                                                   gAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGc                                                                 >  1:497099/1‑150 (MQ=255)
                                                                                    aaaaGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGcc                                                                >  1:1602845/1‑150 (MQ=255)
                                                                                    aaaaGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGcc                                                                <  2:190986/150‑1 (MQ=255)
                                                                                    aaaaGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACACTCGCGGGGCCGGGATTATACCAAcc                                                                >  1:588166/1‑150 (MQ=255)
                                                                                    aaaaGGTTGCGAAGCGCGTCACTATTTATTTATATCTTTACCGTAAGAATGCAGAAACCGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGcc                                                                <  2:1939926/150‑1 (MQ=255)
                                                                                       aGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCa                                                             >  2:700981/1‑150 (MQ=255)
                                                                                         gTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATc                                                           <  1:1163275/150‑1 (MQ=255)
                                                                                           tGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCTGGTATAATCCCGGccccgcgag                                                         <  2:1856374/150‑6 (MQ=255)
                                                                                             cGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAg                                                                  <  2:810606/139‑1 (MQ=255)
                                                                                             cGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAg                                                                  >  1:810606/1‑139 (MQ=255)
                                                                                              gAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATccgccg                                                      >  2:740927/1‑150 (MQ=255)
                                                                                              gAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATccgccg                                                      >  2:713886/1‑150 (MQ=255)
                                                                                                  cgcgTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGc                                                  <  1:976055/150‑1 (MQ=255)
                                                                                                   gcgTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGcc                                                 >  2:1375498/1‑150 (MQ=255)
                                                                                                   gcgTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGcc                                                 <  2:1955558/150‑1 (MQ=255)
                                                                                                         cTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAggg                                           >  2:640157/1‑150 (MQ=255)
                                                                                                         cTATTTATTATTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAggg                                           <  1:144176/150‑1 (MQ=255)
                                                                                                          tatttattTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGgc                                          <  2:368484/150‑1 (MQ=255)
                                                                                                               atttTTATCTTTACCGTAAGAATGCAGAAACAGCGGCCCAGCCGCTGTTTATTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGTGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGgccagcc                                     >  2:190766/1‑150 (MQ=255)
                                                                                                               atttTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGgccagcc                                     <  1:529643/150‑1 (MQ=255)
                                                                                                                  tttATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACg                                  <  1:1812021/150‑1 (MQ=255)
                                                                                                                    tATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGAc                                <  1:1648880/150‑1 (MQ=255)
                                                                                                                       cTTTACCGTAAGAATGCAGAAACAGCGGACAAGCCGCTGTTTCTTTTTCTTTAATAAAAAGCAATTATACAGTTACAACTGATTGTACTCTCGCGTGGCCGGGATTATACCAGCCCAATCCTCCGTAGCCATAGGGCCAGCAACGAACGc                             >  1:290769/1‑150 (MQ=255)
                                                                                                                       atttCCCTTAATAATGCATAAACCGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGc                             <  2:1663508/149‑1 (MQ=255)
                                                                                                                        tttACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGcc                            >  1:665570/1‑150 (MQ=255)
                                                                                                                           aCCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATa                         <  1:211515/150‑1 (MQ=255)
                                                                                                                            ccGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATaa                        <  1:740927/150‑1 (MQ=255)
                                                                                                                                 aGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCCCGCCCGCCATAAAACCa                   >  1:1725478/1‑150 (MQ=255)
                                                                                                                                  gAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCAt                  <  1:1144416/150‑1 (MQ=255)
                                                                                                                                   aaTGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATg                 <  1:1679777/150‑1 (MQ=255)
                                                                                                                                    aTGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATgg                <  1:1091844/150‑1 (MQ=255)
                                                                                                                                     aaaaGAAACAGCGGCTGGTCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGCCCGCCATAAAACCATGGa               >  2:1035710/4‑150 (MQ=255)
                                                                                                                                        aGAAACAGCGGCTGGTCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGACAACCACGACCGCCATAATACAATGGATCa            >  1:62202/1‑150 (MQ=255)
                                                                                                                                         taaaCAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCTCCATAAAACCATGGATCaa           <  1:230517/149‑1 (MQ=255)
                                                                                                                                         gAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCaa           >  2:1947648/1‑150 (MQ=255)
                                                                                                                                          aaaCAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATACAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAAc                     >  1:1896839/1‑139 (MQ=255)
                                                                                                                                           aaCAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTAACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCCCCGTCGCCATAGGGCCCGCCACGACCGCCATAAAACCATGGCTCAATc         >  1:1735174/1‑150 (MQ=255)
                                                                                                                                            aCAGCGGGCCCGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGCCCGCCATAAAACCATGGATCAATCg        >  2:205171/1‑150 (MQ=255)
                                                                                                                                              aGCGGACCAGCCGCTGTTTCTTTTTCTTTTAAAACAAGCAATTATTCAGTTAAAACTGTTTGTACTCTCGCGGGGCCGGGATTATAACAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGc      >  1:508991/1‑150 (MQ=255)
                                                                                                                                              aGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGc      >  2:9207/1‑150 (MQ=255)
                                                                                                                                                cGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTg    <  1:1204581/150‑1 (MQ=255)
                                                                                                                                                 ggACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGTCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGa   >  2:1532750/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GTTCGTCGTCATCGCAACCCCAAATGATATACATGCCGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAG  >  NC_000913/1889768‑1890063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: